• KSBB Journal
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• v.23 no.3
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• pp.231-238
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• 2008

The second family member of tissue inhibitors of matrix metalloproteinases, TIMP-2, is a 21kDa protein which inhibits matrix metalloproteinases 2 (MMP-2). Expression of mammalian proteins in E. coli often forms inclusion bodies that are made up of mis-folded or insoluble protein aggregates. The requirement for the formation of 6 disulfide bonds in the process of the TIMP-2 folding is likely to be incompatible with the reducing environment of E. coli. However, this incompatibility can be often overcome by introducing a mutagenesis that could lead to enhancement of the protein solubility. In this reason, we have attempted to express the soluble TIMP-2 in E. coli by applying a modified staggered extension process (StEP), one of the in vitro PCR-based recombinant mutagenesis methods, and error-prone PCR. C-terminally located CAT fusion protein with respect to mutated TIMP-2 proteins enables us to differentiate the soluble TIMP-2 from the insoluble in E. coli by virtue of chloramphenicol resistance. According to this scheme, E. coli harboring properly-folded CAT fused to TIMP-2 protein was selected, and some of the resulting colonies exhibited an enhanced, soluble expression of TIMP-2 compared to the wild type, implying (i) the StEP technique is successfully employed to enhance the proper folding thereby increasing the solubility of TIMP-2, and (ii) the CAT dependent screening may be a simple and effective method to differentiate the soluble protein expression in E. coli.

• Asia-Pacific Journal of Business Venturing and Entrepreneurship
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• v.13 no.2
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• pp.63-77
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• 2018

In the rapidly to change global market in recent years, as the era of merging and integrating industries and the evolution of technology have come to an era in which everything can not be solved as a single company, it is evolving into competition for the enterprise network rather than the competition for the enterprise unit. In a competitive business environment, it is necessary to provide not only for the efforts as an individual companies but also the mutual development efforts to enhance output through the innovation activities based on the interrelationship with the business partners. In spite of the recent efforts and research through core competencies and innovation activities, some of business activities were unable to achieve enough progress in business performance and this study mainly focused to improve business performance for those companies. This study targeted CEOs and Directors who participates in "manufacturing performance innovation partnership project" carried by The foundation of Large, SMEs, Agriculture, Fisheries cooperation Korea and studied the influences of innovation activities to the core competencies and business performance. Detailed variables in this study were extracted from the previous research and used for verification. The study is designed to determine the influence of individual innovation activities to the core competencies and business performance. Innovation activities as a parameter, the relationship between core competencies and business performance was examined. In the examination of the innovation activities as a meditated effect, those activities carried by SMEs (Collaboration in Technology, Manufacturing, and Management innovations with Large Scale Business) through partnership in manufacturing innovation is significantly related business performance. Therefore, the result reveals that the individual SMEs are having own limitation in the achievement of significant progress in business performance with their own capabilities, and using the innovation activities act as catalyst through the collaboration with large scale businesses would result significant progress in business performance. Mutual effort in collaborative innovation activities between large scale businesses and SMEs is one of the most critical issues in recent years in Korea and the main focus of this study is to provide analysis which demonstrates where the SMEs are required to focus in their innovation activities.

• Korean Journal of Environmental Agriculture
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• v.32 no.3
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• pp.193-200
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• 2013

BACKGROUND: Plant factory system of an artificial light type using Light-Emitting Diodes (LEDs), fluorescent light, or metal halide lamp instead of sun light is an ultimated method for plant production without any pesticides regardless of seasonal changes. The plant factory is also completely isolated from outside environmental conditions such as a light, temperature, or humidity compared to conventional greenhouse. Light-environment control such as a quality or quantity in the plant factory system is essential for improving the growth and development of plant species. However, there was little report that the effects of various light qualities provided by LEDs on Ledebouriella seseloides growth under the plant factory system. METHODS AND RESULTS: Ledebouriella seseloides seedlings transplanted at urethane sponge were grown in the plant factory system of a horizontal type with LED artificial lights for 90 days. Yamazaki solution for hydroponic culture of the seedlings was regularly irrigated by the deep flow technique (DFT) system on the culture gutters. Electrical Conductivity (EC) and pH of the solution was recorded at 1.4 ds/m and 5.8 in average, respectively during the experimental period. Number of unfolded leaves, leaf length, shoot fresh and dry weight of the seedlings were three times measured in every 30 days after beginning of the experiment. Blue LEDs, red LEDs, and fluorescent lights inside the plant factory were used as light sources. Conventional fluorescent lamps were considered as a control. In all the treatment, light intensity was maintained at $100{\mu}mol/m^2/s$ on the culture bed. Fresh weight of the seedlings was 3.7 times greater in the treatment with the mixture radiation of fluorescent light and blue+red LEDs (1:3 in energy ratio; Treatment FLBR13) than in fluorescent light treatment (Treatment FL). In FLBR13 treatment, dry weight per seedling was two times greater than in FL or BR11 treatment of blue+red LEDs (1:3 in energy ratio; Treatment BR11) during the culture period. Increasing in number of unfolded leaves was also significantly affected by the FLBR13 treatment comparing with BR11 treatment. CONCLUSION(S): Hydroponic culture of Ledebouriella seseloides seedlings was successfully achieved in the plant factory system with mixture lights of blue, red LEDs and fluorescent lights. Shoot growth of the seedlings was significantly promoted by the FLBR13 with the mixture radiation of fluorescent light, blue, and red LEDs under 1:3 mixture ratio of blue and red LEDs during the experimental period compared to conventional light conditions.

• Journal of Animal Science and Technology
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• v.46 no.3
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• pp.459-468
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• 2004

An experiment was conducted to establish comparison of ventulation efficiency in an enclosed and conventional growing-finishing pig house. The main results of the experiment are as follows : In the established temperature was sustained at the level of summer 24.8${\sim}$29.1$^{\circ}C$, winter 17.9${\sim}$23.1$^{\circ}C$ during the experimental period of enclosed growing-finishing pig house, and conventional growing-finishing pig house was at the lovel of summer 24.7${\sim}$32.3$^{\circ}C$, winter 14.5${\sim}$18.2$^{\circ}C$ during the experimental period respectively. As for the results of dertimental gas(ammonia) concentration ratio analysis, while the conventional pig house sustained of summer 9.3${\sim}$16.9 mg/$\ell$ level, enclosed growing-finishing pig house sustained of summer 7.9${\sim}$16.1 mg/$\ell$, and the latter one is lower than that of the conventional growing-finishing pig house. Air flow rate on the floor level which is the low part of pen and the active area of pigs in the enclosed growing and finishing pig house during winter was measured at 0 to 0.87 m/s at the 0.01 to 2.73 m/s at the maximum ventilation efficiency. As for breeding pigs in summer, the pigs from the conventional pig house weighed 100.2kg, on the other hand, the pigs from enclosed growing-finishing pig house weighed 107.3 kg ; the differnce between the two kinds was about 7 kg. This was because the most adequate environment, which was not influenced by the exterior atmosphere, was offered to the pigs from enclosed growing-finishing pig house, and all of this could reduce pigs stress effectively.

• Journal of Broadcast Engineering
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• v.22 no.6
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• pp.829-849
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• 2017

Due to the large-scale TV display, the convergence of broadcasting and broadband, and the advancement of signal compression and transmission technology, terrestrial digital broadcasting has evolved into UHD broadcasting capable of providing simultaneous broadcasting of fixed UHD and mobile HD. The Korean standard for terrestrial UHDTV broadcasting is based on ATSC 3.0, the broadcasting standard of North America. The terrestrial UHDTV broadcasting standard chose that as a new AV codec standard, HEVC video codec which can compress with higher efficiency compared to AVC, and MPEG-H 3D audio codec for realistic audio. Also, DASH and MMT are adopted as transmission format instead of MPEG-2 TS to support broadband as well as broadcasting network, and in order to provide 4K UHD/mobile HD service simultaneously ROUTE multiplexing technology is applied. In this paper, we propose an audio/video encoder, which is required to provide HDR/WCG supported high quality video service, 10.2 channel/4 object supporting stereo sound service, fixed UHD and mobile HD simultaneous broadcasting service based on ATSC3.0, also we implemented the ATSC 3.0 LDM system for ROUTE/DASH packager, multiplexing system and physical layer transmission/reception, and verified the service ability by applying it to real time broadcast environment.

• Korean Journal of Soil Science and Fertilizer
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• v.38 no.3
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• pp.150-156
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• 2005

The increasing emission of greenhouse gases may change agricultural environment. The agronomic productivity will depend upon change of temperature, precipitation, solar radiation and fertilization. This study was conducted to investigate greenhouse gas emission with irrigation water depth in paddy field. Area of each experiment plot is $70m^2$, Three treatments with three replications were carried out in this experiment, which was laid out as randomized complete block design. The treatments of irrigation water were maximum field water capacity and 4 and 8 cm depth. The application rate of fresh rice straw was $8,000kg\;ha^{-1}$ in combination with chemical fertilizers ($110kg\;N\;ha^{-1}$, $45kg\;P_2O_5\;ha^{-1}$ and $57kg\;K_2O\;ha^{-1}$). The $CH_4$ emission was highest at 32 days after rice transplanting with rice straw treatment. The $CH_4$ emission in the plot of maximum field water capacity was lower compared with 4 and 8 cm of irrigation depth. $CH_4$ and $N_2O$ emission under different water depth in the paddy field were 30 and $1.52kg\;ha^{-1}$ at 8 cm depth, 281 and $1.71kg\;ha^{-1}$ at 4 cm depth, and 219 and $2.01kg\;ha^{-1}$ at water saturated condition. The total emission of greenhouse gases equivalent to $CO_2$ emission with rice straw application were $6,939kg\;CO_2\;ha^{-1}$ at 8 cm depth plot, $6,431kg\;CO_2\;ha^{-1}$ at 4 cm depth plot and $5,222kg\;CO_2\;ha^{-1}$ at water saturated condition. The GWPs without rice straw application were $4,449kg\;CO_2\;ha^{-1}$ at 8 cm depth plot, $3,702kg\;CO_2\;ha^{-1}$ at 4 cm depth plot and $4,579kg\;CO_2\;ha^{-1}$ at water saturated condition.

• Journal of Sericultural and Entomological Science
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• v.51 no.1
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• pp.1-8
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• 2013

This study was conducted for investigation of habitat and development of indoor-rearing conditions of peacock butterfly, Inachis io (Linnaeus). Three different region, Mt. Kwangduk-san, Mt. Hae-san and Mt. Taebaek-san in Kangwon-do, was selected for investigation of domestic distribution and ecological environment of peacock butterfly. In result, there are many butterflies in the only two region, Mt. Kwangduk-san and Mt. Hae-san. On 16th April, 4 overwintered butterflies were observed in Mt. Hae-san. At the end of June and early in July, 51 individual next generation's butterflies were observed in Mt. Kwangduk-san and Mt. Hae-san. For development of indoor-rearing conditions, collected each 15 male and female peacock butterflies was reared in a room condition. Female butterflies laid eggs in the egg cluster on the underside of host-plant's leaves, Urtica angustifolia Fisch., in the ovipositioning room. We took 11 egg cluster with average $404.6{\pm}23.6$ eggs. Hatchablity of eggs was 92.3%, and the eggs hatched within $5.1{\pm}0.9$ days from the day of oviposition under high temperature, long day condition($25^{\circ}C$, 14L:10D). The larval period was $18.3{\pm}1.0$ days under high temperature, long day condition($25^{\circ}C$, 14L:10D), showing 81.0% pupal ratio. The head width of each developmental larval stage were $0.37{\pm}0.01mm$(1st instar), $0.66{\pm}0.02mm$(2nd instar), $1.07{\pm}0.05mm$(3rd instar), $1.81{\pm}0.07$(4th instar), $2.76{\pm}0.08$(5th instar). The pupal period was $8.4{\pm}0.6$ days, and the emergence rate was 87.5%.

• Clean Technology
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• v.17 no.1
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• pp.48-55
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• 2011

The application of fibrous activated carbon (FAC)-titanium dioxide ($TiO_2$) hybrid system has not been reported yet for the control of malodorous dimethyl sulfide (DMS) at residential environmental levels. Accordingly, the current study was designed not only to characterize this hybrid system using x-ray diffraction method, particulate surface measurement and Fourier transform Infrared (FTIR) method, but also to evaluate its adsorptional photocatalytic activity (APA) for the DMS removal. The physical/surface characteristics of FAC-$TiO_2$ which was prepared in this study suggested that the hybrid material might have certain APA for DMS. The Brunauer-Emmett-Teller (BET) specific area, total pore volume, micropore volume and mesopore volume decreased all as the $TiO_2$ amounts coated on FAC increased, whereas the reverse was true for average pore diameter. $TiO_2$ coated onto FAC did not influence the adsorptional activity of FAC for the DMS input concentration of 0.5 ppm. The APA test of the hybrid material presented that the initial removal efficiencies of DMS were 93, 78, 71 and 57% for the flow rates of 0.5, 1.0, l.5 and 2.0 L/min, respectively, and they decreased somewhat 2 h after the experiment started and kept almost constant for the rest experimental period. Under this pseudo-equilibrium condition, the DMS removal efficiencies were 78, 58, 53 and 36% for the four flow rates, respectively. Meanwhile, there were no significant byproducts observed on the surfaces of the hybrid material. Consequently, this study suggests that, under the experimental conditions used in the present study, the hybrid material can be applied for DMS at residential environment levels without being interfered by any byproducts.

• Korean Journal of Food Science and Technology
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• v.43 no.2
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• pp.243-248
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• 2011

The prevalence and distribution of hazardous microorganisms were investigated from the major perilla cultivation area at Milyang, Gyeongnam province, Korea. Aerobic plate count (APC) and coliform count of perilla leaves were 4.82 log CFU/g and 3.85 log CFU/g, respectively. E. coli, S. aureus and B. cereus were detected in 3.0% (4/114), 7.9% (9/114) and 46.5% (53/114) of examined perilla leaves. However, E. coli O157:H7, Salmonella spp, and L. monocytogenes were not detected. The distribution of hazardous microorganisms in perilla leaf cultivation environment were compared and the concentration of APC and coliform counts were more than 3.0 log CFU/(mL, g, $100cm^2$, hand) from most of the samples. S. aureus were detected from irrigation water, packing table, packing vinyl, hand, and clothes. Also, B. cereus was frequently detected from the examined samples. Especially, packing table and collection container were contaminated with maximum 5.5 log $CFU/100cm^2$ of B. cereus. Good Agricultural Practice (GAP) system should be introduced to farms to enhance the safety of perilla leaves.

• International Journal of Ocean System Engineering
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• v.2 no.3
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• pp.185-194
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• 2012

A Macroscopic combination of two or more distinct materials is commonly referred to as a "Composite Material", having been designed mechanically and chemically superior in function and characteristic than its individual constituent materials. Composite materials are used not only for aerospace and military, but also heavily used in boat/ship building and general composite industries which we are seeing increasingly more. Regardless of the various applications for composite materials, the industry is still limited and requires better fabrication technology and methodology in order to expand and grow. An example of this is that the majority of fabrication facilities nearby still use an antiquated wet lay-up process where fabrication still requires manual hand labor in a 3D environment impeding productivity of composite product design advancement. As an expert in the advanced composites field, I have developed fabrication skills with the use of machinery based on my past composite experience. In autumn 2011, the Korea government confirmed to fund my project. It is the development of a composite sanding machine. I began development of this semi-robotic prototype beginning in 2009. It has possibilities of replacing or augmenting the exhaustive and difficult jobs performed by human hands, such as sanding, grinding, blasting, and polishing in most often, very awkward conditions, and is also will boost productivity, improve surface quality, cut abrasive costs, eliminate vibration injuries, and protect workers from exposure to dust and airborne contamination. Ease of control and operation of the equipment in or outside of the sanding room is a key benefit to end-users. It will prove to be much more economical than normal robotics and minimize errors that commonly occur in factories. The key components and their technologies are a 360 degree rotational shoulder and a wrist that is controlled under PLC controller and joystick manual mode. Development on both of the key modules is complete and are now operational. The Korean government fund boosted my development and I expect to complete full scale development no later than 3rd quarter 2012. Even with the advantages of composite materials, there is still the need to repair or to maintain composite products with a higher level of technology. I have learned many composite repair skills on composite airframe since many composite fabrication skills including repair, requires training for non aerospace applications. The wind energy market is now requiring much larger blades in order to generate more electrical energy for wind farms. One single blade is commonly 50 meters or longer now. When a wind blade becomes damaged from external forces, on-site repair is required on the columns even under strong wind and freezing temperature conditions. In order to correctly obtain polymerization, the repair must be performed on the damaged area within a very limited time. The use of pre-impregnated glass fabric and heating silicone pad and a hot bonder acting precise heating control are surely required.