• Tunnel and Underground Space
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• v.24 no.1
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• pp.81-88
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• 2014

In the course of performing the stability analysis of rock structures, there are times when the strength of the Hoek-Brown rock mass needs to be understood in terms of the internal friction angle and cohesion. In this case, the original Hoek-Brown criteion, giving the relationship between ${\sigma}_1$ and ${\sigma}_3$ at failure, have to be transformed to the corresponding Mohr envelope. A new approach to derive the Mohr envelope of the Hoek-Brown criterion is suggested in this study. The new method is based on the Londe's transformation making the stress components dimensionless. The correctness of the derivation leading to the new ${\tau}-{\sigma}$ relationship is confirmed by comparing the calculation results with the Bray's solution through a verification example.

• Biomolecules & Therapeutics
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• v.20 no.2
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• pp.171-176
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• 2012

HaCaT cells are the immortalized human keratinocytes and have been extensively used to study the epidermal homeostasis and its pathophysiology. T helper cells play a role in various chronic dermatological conditions and they can affect skin barrier homeostasis. To evaluate whether HaCaT cells can be used as a model cell system to study abnormal skin barrier development in various dermatologic diseases, we analyzed the gene expression profile of epidermal differentiation markers of HaCaT cells in response to major T helper (Th) cell cytokines, such as $IFN{\gamma}$, IL-4, IL-17A and IL-22. The gene transcriptional profile of cornified envelope-associated proteins, such as filaggrin, loricrin, involucrin and keratin 10 (KRT10), in HaCaT cells was generally different from that in normal human keratinocytes (NHKs). This suggests that HaCaT cells have a limitation as a model system to study the pathophysiological mechanism associated with the Th cell cytokine-dependent changes in cornified envelope-associated proteins which are essential for normal skin barrier development. In contrast, the gene transcription profile change of human ${\beta}2$-defensin (HBD2) in response to $IFN{\gamma}$, IL-4 or IL-17A in HaCaT cells was consistent with the expression pattern of NHKs. $IFN{\gamma}$ also up-regulated transglutaminase 2 (TGM2) gene transcription in both HaCaT cells and NHKs. As an alternative cell culture system for NHKs, HaCaT cells can be used to study molecular mechanisms associated with abnormal HBD2 and TGM2 expression in response to $IFN{\gamma}$, IL-4 or IL-17A.

• Journal of the Korea Society for Simulation
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• v.17 no.4
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• pp.89-97
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• 2008

In wireless personal area networks (WPANs), the successful design of channel time allocation algorithm is a key factor in guaranteeing the various quality of service (QoS) requirements for the stringent real-time constraints of multimedia services. In this paper we propose a channel time allocation algorithm for achieving a high quality transmission and high channel error tolerance of MPEG stream in the IEEE 802.15.3 high-rate wireless PANs. Our algorithm exploits the characteristics of MPEG stream. When a new MPEG stream arrives, a DEV models it by the traffic envelope and delivers the traffic envelope to the piconet coordinator (PNC) along with the channel time request. The PNC performs channel time allocation according to the envelope. Performance of the proposed scheme is investigated by simulation and analysis. Our results show that compared to conventional scheme, the proposed scheme is very effective and provides a good performance under typical channel error conditions.

• Animal cells and systems
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• v.2 no.3
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• pp.341-349
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• 1998

To analyze the effects of surfactants on the biosynthesis of galactolipid and the composition of fatty acids, the chloroplast envelope and thylakoid membrane were cultivated in medium treated with anionic surfactants, such as linear alkylbenzene sulfonate (0.002%, LAS), a-olefin sulfonate (O.01%, AOS), and sodium lauryl ether sulfate (0.08%, SLES), respectively. During the cultivation, the chloroplast envelope and thylakoid membrane were isolated from the cells collected at the early and middle phase of the culture and the contents of their fatty acid composition were compared with the control. When treated with surfactants, the contents of total lipid MDGD methylesters, and DGDG methylesters decreased significantly when compared with the control. It was also confirmed that more unsaturated fatty acids were involved in the biosynthesis of galactolipid. The fatty acids utilized in the biosynthesis of MGDG were in the chloroplast envelope and in the control, and linoleic acid in LAS, linolenic acid and oleic acid in AOS, and linolenic acid and oleic acid in SLES. The fatty acids in the biosynthesis of DGDG were linolenic acid and oleic acid in the control linolenic acid and stearic acid in LAS, oleic acid and linolenic acid in AOS, oleic acid and linolenic acid in SLES. In the thylakoid membrane, the major fatty acids in the biosynthesis of MGDG were linolenic acid and oleic acid in the control, oleic acid and linolenic acid in LAS, linolenic acid and linoleic acid in AOS, linolenic acid and palmitoleic acid in SLES. The fatty acids in the biosynthesis of DGDG were linolenic acid and oleic acid in the control, oleic acid and linolenic acid in LAS, linolenic acid and linoleic acid in AOS, palmitoleic acid and oleic acid in SLES.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2004.03a
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• pp.848-853
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• 2004

Regarding to the project SUAV (Smart Unmanned Aerial Vehicle) in KARI (Korea Aerospace Research Institute), several engine configurations has been evaluated. However it's not an easy task to collect all the necessary data of each engine for the analysis. Usually, some kind of modeling technique is required in order to determine the unknown data. In the present paper a qualitative method for reverse engineering is proposed, in order to identify some design patterns and relationships between parameters. The method can be used to estimate several parameters that usually are not provided by the manufacturer. The method consists of modeling an existing engine and through a simulation, compare its transient behavior with its operating envelope. In the simulation several parameters such as thermodynamics, performance, safety and mechanics concerning to the definition of operation-envelope, have been discussed qualitatively. With the model, all engine parameters can be estimated with acceptable accuracy, making possible the study of dependencies among different parameters such as power-turbine total inertia, TIT, take-off time and part load, in order to check if the engine transient performance is within the design criteria. For more realistic approach and more detailed design requirements, it will be necessary to enhance the compressor map first, and more realistic estimated values must be taken into account for intake-loss, bleed-air and auxiliary power extraction. The relative importance of these “unknown” parameters must be evaluated using sensitivity analysis in the future evaluation. Moreover, fluid dynamics, thermal analysis and stress analysis necessary for the resulting life assessment of en engine, will not be addressed here but in a future paper. With the methodology presented in the paper was possible to infer the relationships between operation-envelope and engine parameters.

• ALGAE
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• v.33 no.4
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• pp.305-318
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• 2018

In Trachelomonas hispida, the adult envelope that surrounds the monad is oval in shape and covered with spines. Development of the lorica is an interesting, but poorly known phenomenon. We observed in detail the formation of spineless envelopes of T. hispida using light microscopy and scanning electron microscopy. The results showed that young cells formed delicate and net-like envelopes. The structure changed with age, and mature specimens had solid, thick loricae with small pores. As the loricae aged, they changed their shape, and rope-like fibres from the external layer started to unwind, progressing from the apical pore, exposing the underlying net-like structure. X-ray spectrometry showed that Fe was the major mineral component in young and mature loricae, whereas old loricae did not contain Fe salts, although they did contain a high number of Mn compounds. We also noticed a different pattern of mineralization process in the envelopes. Apart from the even distribution of mineral deposition over the entire lorica, we observed that it started from the apical pore and ended at the posterior end. There was considerable morphological variation in envelope shape and ornamentation, which had collars and folds around the apical pore and process at the posterior end. This suggested that many varieties of T. hispida should be taxonomically reappraised. We also discuss a hypothetical role for the spines on lorica surfaces in aquatic ecosystems.

• Journal of the Architectural Institute of Korea Structure & Construction
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• v.35 no.6
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• pp.153-164
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• 2019

The presence of thermal bridges in a building envelope cause additional heat loss which increases the heating energy. Given that a higher building insulation performance is required in these cases, the heat loss via thermal bridges is a high proportion of the total heat energy consumption of a building. For the dry exterior insulation system that uses mullions and transoms to fix insulation and exterior materials such as stone and metal sheet, the occurrence of thermal bridges at mullions and transoms is one of the main reasons for heat loss. In this study, a dry exterior insulation system using the truss insulation frame (TIF) was proposed as an alternative to metal mullions. To evaluate the building envelope performance, structural, air-leakage, water-leakage, fire-resistance, thermal, and condensation risk tests were conducted. In addition, the annual energy consumption associated with heating and cooling was calculated, including the linear thermal transmittance of the thermal bridges. As a result, the dry exterior insulation system using TIF achieved the allowable value for all tests. It was also determined that the annual heating load of a building was reduced by 36.7 % when the TIF dry exterior insulation system was used, relative to the dry exterior insulation system using steel pipes without additional insulations.

• The Journal of the Acoustical Society of Korea
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• v.28 no.6
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• pp.526-533
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• 2009

This paper proposes a formant synthesis method of Haegeum sounds using cepstral envelope for spectral modeling. Spectral modeling synthesis (SMS) is a technique that models time-varying spectra as a combination of sinusoids (the "deterministic" part), and a time-varying filtered noise component (the "stochastic" part). SMS is appropriate for synthesizing sounds of string and wind instruments whose harmonics are evenly distributed over whole frequency band. Formants extracted from cepstral envelope are parameterized for synthesis of sinusoids. A resonator by Impulse Invariant Transform (IIT) is applied to synthesize sinusoids and the results are bandpass filtered to adjust magnitude. The noise is calculated by first generating the sinusoids with formant synthesis, subtracting them from the original sound, and then removing some harmonics remained. Linear interpolation is used to model noise. The synthesized sounds are made by summing sinusoids, which are shown to be similar to the original Haegeum sounds.

• Journal of the Korea institute for structural maintenance and inspection
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• v.10 no.2
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• pp.59-67
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• 2006

The aim of this study is to evaluate the design table for envelope curve analysis of base isolated buildings, which represent the period of base isolated buildings and the lateral displacement of base isolation devices. For the construction of design table, $V_E$ spectrum, which represents the energy, is developed instead of acceleration of seismic hazard. Based on the seismic coefficient of UBC 97, boundary period $T_G$ and maximum velocity response $V_0$ are proposed considering Korea seismic hazard. Using $T_G$ and $V_0$, finally, $V_E$ spectrum is developed for the four types of soil conditions. Base on the $V_E$ spectrum, design table for envelope curve analysis is also developed for soil types.

• Clinical and Experimental Vaccine Research
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• v.11 no.3
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• pp.249-263
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• 2022

Purpose: Lassa fever is a zoonotic acute viral hemorrhagic disease caused by Lassa virus (LASV). There is currently no licensed vaccine for the prevention of the disease. This study is aimed at discovering immunodominant epitopes from the envelope glycoprotein of the Lassa mammarenavirus and designing of a multi-epitope vaccine candidate (VC). Materials and Methods: The amino acid sequences of the envelope glycoprotein of 26 strains of LASV from five countries were selected. After evaluation for antigenicity, immunogenicity, allergenicity, and toxicity, immunodominant CD8, CD4, and linear B lymphocytes were also selected. The selected epitopes were modelled and a molecular docking with the appropriate major histocompatibility complex (MHC) proteins was performed. Using an adjuvant and linkers, a multi-epitope VC was designed. The VC was evaluated for its physicochemical and immunological properties and structurally refined, validated, and mutated (disulphide engineering). The complex formed by the VC and the toll-like receptor-4 receptor was subjected to molecular dynamic simulation (MDS) followed by in silico cloning in a plasmid vector. Results: A VC with 203 sequences, 22.13 kDa weight, isoelectric point of 9.85 (basic), instability index value of 27.62, aliphatic index of 68.87, and GRAVY value of -0.455 (hydrophilic) emerged. The VC is predicted to be non-allergenic with antigenicity, MHC I immunogenicity, and solubility upon overexpression values of 0.81, 2.04, and 0.86 respectively. The VC also has an estimated half-life greater than 10 hours in Escherichia coli and showed stability in all the parameters of MDS. Conclusion: The VC shows good promise in the prevention of Lassa fever but further tests are required to validate its safety and efficacy.