• Title/Summary/Keyword: Engineering process

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Electrical Biosensor Of Kinase Assay on the MWCNT Nanoelectrode

  • Lee, Jae-Shin;Lee, Seok-Jae;Park, Jong-Pil;Park, Tae-Jung;Kim, Do-Hyun;Lee, Sang-Yup;Jung, Dae-Hwan;Jung, Hee-Tae;Kim, Jin-Hee;Kwon, Seong-Ku
    • 한국정보디스플레이학회:학술대회논문집
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    • 2004.08a
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    • pp.1193-1196
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    • 2004
  • We have demonstrated the use of MWCNT as a nanoscale probe to monitor the activity of enzyme kinase. To immobilize the substrate peptide using carbodiimide chemistry, plasma or strong acid treatments were used to induce carboxyl groups on the sidewall of MWCNTs. After the susbtrate peptide immobilization, increase of conductance from MWCNT devices was observed. When peptide modified MWCNTs react with enzyme kinase, conductance decreases by several orders of magnitude, and this conductance change can be explained by the phosphorylation reaction of enzyme kinase. When the sample was incubated with phosphatase to dephosphorylate the substrate peptide, nearly complete recovery of the conductance signal has been observed. 4 for 6 devices appeared the same trends. So, we can confirm that we have monitored the kinase activity on the MWCNT surface by electrical detection.

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Process Design of Conical Roll-Shaping for Fabrication of Variable Curvature Spiral Blade (가변곡률을 가진 나선형 블레이드 제작을 위한 원추형 롤 성형 공정설계)

  • Yang, Sungmoon;Shim, Dosik;Ji, Hoseong;Baek, Joonho;Kim, Bongsik;Ahn, Seokyoung;Park, Sanghu
    • Journal of the Korean Society for Precision Engineering
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    • v.33 no.11
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    • pp.911-918
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    • 2016
  • A conical roll-shaping process was proposed for fabrication of a metallic spiral blade applied to a small-scale wind turbine system. A spiral blade has continuously different curvatures, with a range of 100 to 350 mm radius. To fabricate this complex shape, we developed a conical roll-shaping process having two main conical rollers for feeding a blank sheet, and two cylindrical side rollers for control of local bending. For clear understanding of the process parameters, numerical analyses were conducted using a commercial code, Pam-Stamp. This study optimized the effects of process parameters, such as gap and angle between the main rollers and side rollers, and also the movement of side rollers. In order to increase the forming efficiency, a central rotation point was also calculated by the analytical approach. This developed rolling process can thus be utilized in a sheet metal forming process for obtaining spirally curved sheet metal shapes.

Development of Expected Loss Capability Index Using Reflected Normal Loss Function (역정규 손실함수를 이용한 기대손실 능력지수의 개발)

  • Chun, Dong-Jin;Chung, Young-Bae
    • Journal of Korean Society of Industrial and Systems Engineering
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    • v.40 no.1
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    • pp.41-49
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    • 2017
  • Process quality control, which prevents problems and risks that may occur in products and processes, has been recognized as an important issue, and SPC techniques have been used for this purpose. Process Capability Index (PCI) is useful Statistical Process Control (SPC) tool that is measure of process diagnostic and assessment tools widely use in industrial field. It has advantage of easy to calculate and easy to use in the field. $C_p$ and $C_{pk}$ are traditional PCIs. These traditional $C_p$ and $C_{pk}$ were used only as a measure of process capability, taking into account the quality variance or the bias of the process mean. These are not given information about the characteristic value does not match the target value of the process and this has the disadvantage that it is difficult to assess the economic losses that may arise in the enterprise. Studies of this process capability index by many scholars actively for supplement of its disadvantage. These studies to evaluate the capability of situation of various field has presented a new process capability index. $C_{pm}$ is considers both the process variation and the process deviation from target value. And $C_{pm}{^+}$ is considers economic loss for the process deviation from target value. In this paper we developed an improved Expected Loss Capability Index using Reflected Normal Loss Function of Spring. This has the advantage that it is easy to realistically reflect the loss when the specification is asymmetric around the target value. And check the correlation between existing traditional process capability index ($C_{pk}$) and new one. Finally, we propose the criteria for classification about developed process capability index.

Production of Lyopilized Culture of Lactobacillus acidophilus with Preserving Cell Viability

  • Kang, Moo-Heon;Vibhor Saraswat;Lee, Jeewon;Park, Young-Hoon
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.4 no.1
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    • pp.36-40
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    • 1999
  • Optimal lyophilization process was developed for manufacturing the dried product of Lactobacillus acidophilus with high cell viability. Three major factors, freezing rate, specific surface area of samples, and stabilizer type and their synergy were shown to play a crucial role in the development of an effective lyophilization process. Finally we found an optimal combination among three process parameters mentioned above; an exceptionally high cell survival percentage of 90% was achieved using the 8.28 cm-1 specific surface area of samples, slow freezing rate, and a stabilizer composition of 4% skim milk +1% glycerol +0.1% calcium chloride.

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Implementation of the joint capability integration and development environment using CASE tool (전산지원 시스템 엔지니어링 도구를 이용한 합동능력 통합 및 개발 환경 구축 사례)

  • Kim, Jin Ill;Park, Jong Seon
    • Journal of the Korean Society of Systems Engineering
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    • v.9 no.2
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    • pp.69-82
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    • 2013
  • US DoD operated JCIDS(Joint Capability Integration and Development System) for top down requirement generation. Although the JCIDS can be a good practice for the countries which are trying to shift from bottom up to top down requirement generation, it contains many processes related with review and approval. In this study we structured a joint capability integration and development process from the JCIDS eliminating the organization dependent review or approval process so that it can be applied to any organization with some modification. Furthermore we implemented the process in the computer aided systems engineering tool, Cradle, for convenient use of the process. The result of this study can provide a basic process for top down capability development, and an efficient why of doing each element of the process using CASE tool.

Identification of the process in closed-loop control system

  • Oura, Kunihiko;Akizuki, Kageo;Hanazaki, Izumi
    • 제어로봇시스템학회:학술대회논문집
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    • 1994.10a
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    • pp.140-145
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    • 1994
  • In this paper, we consider a problem to estimate process parameters using input-output data collected from the process operating in closed-loop control system. When orders and delay-time of the process are known correctly, under some conditions of identifying experiments, it is reported that accurate identification results can be obtained by applying prediction error method. To get accurate estimates, it is necessary to know orders and delay-time of the process. It is difficult to determine them in closed-loop identification, because ill-condition for identification are easily caused by selection of unsuitable order or delay time. Furthermore, the procedures to select orders and delay-time in open-loop identification aren't always available in closed-loop identification. The purpose of this paper is to determine a delay-time under suitable assumption that order of the process are known as the first step.

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