• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2018.05a
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• pp.588-591
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• 2018

Baculovirus was originally isolated from the alfalfa looper and contains a 134-kbp genome with 154 open reading frames (ORF). The major capsid protein VP39 together with some minor proteins forms the nucleocapsid ($21nm{\times}260nm$) that encloses the DNA with p6.9 protein. They are double-stranded, circular, supercoiled DNA molecules in a rod-shaped capsid. Wild-type baculoviruses exhibit both lytic and occluded life cycles that develop independently throughout the three phases of virus replication. Recombinant baculoviruses can transfer their vectors and express their recombinant proteins in a wide range of mammalian cell types. Especially, inclusion of a dominant selectable marker in these baculoviral vectors can express diverse recombinant genes in many cells. Baculoviral vectors were reconstructed with cytomegalovirus (CMV) promoter,uroplakin II promoter, polyhedron promoter, vesicular stomatitis virus G (VSVG), enhanced green fluorescent protein (EGFP), protein transduction domain (PTD) gene and so on. These reconstructed vectors were infected into various cell and cell lines. We performed transfection and expression of these recombinant vectors comparison with other control vectors. From this study, we knew that transfection and expression of these recombinant vectors have higher efficacy than any control vector. This work was supported by a grant from Mid-Career Researcher Program(NRF-2016R1A2B4016552) through the National Research Foundation of Korea(NRF) funded by the Ministry of Science, ICT & Future Planning(MSIP).

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2018.05a
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• pp.584-587
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• 2018

Many viruses including mouse hepatitis virus, corona, measles, and sidbis viruses are known as causative virus of inducing demyelination which means destruction of myelination in nervous system of mice. The purpose of this study is to investigate processing of myelination by co-culture of Schwann cells and neuronal cells and demyelination induced by infection of sindbis virusin rat. Schwann cells and neuronal cells from dorsal root ganglion (DRG) in embryos (E16) of rat were cultured in vitro respectively. The purified neuronal cells with anti-mitotic agents and purified Schwann cells were co-cultured. After that, infection of sindbis virus into this myelinated co-culture system was performed. Myelination and demyelination process were observed using antibody of myelin basic protein meaning presence of myelination.We identified myelination and demyelination processing using antibody of peripheral myelin protein 22 (PMP 22) meaning presence of myelinated neuron. This study was supported by the Basic Research Program through the National Research Foundation (NRF) funded by the Ministry of Science, ICT & Future Planning (NRF-2015R1C1A1A01053484 and 2017R1A2B3005753).

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.3
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• pp.673-680
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• 2017

sump pump is a system that draws in water that is stored in a dam or reservoir. They are used to pump large amounts of water for cooling systems in large power plants, such as thermal and nuclear plants. However, if the flow and sump pump ratio are small, the flow rate increases around the inlet port. This causes a turbulent vortex or swirl flows. The turbulent flow reduces the performance and can cause failure. Various methods have been devised to solve the problem, but a correct solution has not been found for low water level. The most efficient solution is to install an anti-vortex device (AVD) or increase the length of the sump inlet, which makes the flow uniform. This paper presents a computational fluid dynamics (CFD) analysis of the flow characteristics in a sump pump for different sump inlet lengths and AVD types. Modeling was performed in three stages based on the pump intake, sump, and pump. For accurate analysis, the grid was made denser in the intake part, and the grid for the sump pump and AVD were also dense. 1.2-1.5 million grid elements were generated using ANSYS ICEM-CFD 14.5 with a mixture of tetra and prism elements. The analysis was done using the SST turbulence model of ANSYS CFX14.5, a commercial CFD program. The conditions were as follows: H.W.L 6.0 m, L.W.L 3.5, Qmax 4.000 kg/s, Qavg 3.500 kg/s Qmin 2.500 kg/s. The results of analysis by the vertex angle and velocity distribution are as follows. A sump pump with an Ext E-type AVD was accepted at a high water level. However, further studies are needed for a low water level using the Ext E-type AVD as a base.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.3
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• pp.31-40
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• 2017

This paper deals with the high precision of GPS measurement reduction and its implementation on near real-time and kinematic mode for those applications requiring centimeter-level precision of the estimated coordinates, even if target stations are a few hundred kilometers away from their references. We designed the system architecture, data streaming and processing scheme. Intensive investigation was performed to determine the characteristics of the GPS medium-range functional model, IGS infrastructure and some exemplary systems. The designed system consisted of streaming and processing units; the former automatically collects GPS data through Ntrip and IGS ultra-rapid products by FTP connection, whereas the latter handles the reduction of GPS observables on static and kinematic mode to a time series of the target stations' 3D coordinates. The data streaming unit was realized by a DOS batch file, perl script and BKG's BNC program, whereas the processing unit was implemented by definition of a process control file of BPE. To assess the functionality and precision of the positional solutions, an experiment was carried out against a network comprising seven GPS stations with baselines ranging from a few hundred up to a thousand kilometers. The results confirmed that the function of the whole system properly operated as designed, with a precision better than ${\pm}1cm$ in each of the positional component with 95% confidence level.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.12
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• pp.692-700
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• 2016

The study proposed a strategy for supporting North Korea's SOC construction in the future by reviewing domestic and foreign cases. According to the literatureanalysis, we developed a candidate list of technology for SOC of North Korea in terms of Construction Management / economy sectors. The category is North Korea SOC construction support, and the sub-category is North Korea SOC construction support program. The implementation procedures included the following six steps: Preparation, Support for Establishing Construction Industry Base, Support for Establishing Business Management Base, Support for HR Management Base, Business Model Establishment/Expansion, and Monitoring of Management Result. Lastly, we developed twenty-five candidates of detailed technology. In the future, it is necessary to decide a methodology for project implementation by each scenario after selecting a study area, and to develop a more specified scenario.

• The KIPS Transactions:PartA
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• v.18A no.6
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• pp.271-280
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• 2011

Time-Triggered Architecture (TTA), one of real-time software design paradigms which executes tasks in timely manner, has long been advocated as being better suited in fore-sighting system behavior than event-triggered architecture (ETA). To gain this valuable feature of TTA, however, precise task designing process is mandatory. Alternatively, ETA tries to execute tasks whenever paired events are occurred. It provides intuitive and flexible basement to add/remove tasks and, moreover, better response time performance. However ETA is difficult to analyze because system behavior might be different depending on the order of interrupts detected by the system. Many previous researches recommended TTA when developing safety-critical real-time systems, but cost problem of task designing process and insufficient consensus for applying rigorous software engineering practice are still challenging in practice. This paper describes software refactoring process which applying TTA approach into ETA based embedded software in artificial heart system. We implemented dedicated interrupt monitoring program to capture existing tasks' real-time characteristics. Based on the captured information, proper task designing process is done. Real-time analysis using RMA (Rate-Monotonic Analysis) verified that new design guarantees timeliness of the system. Empirical experiments revealed that revised design is as efficient, when measured in terms of system's external output, as the old design and enhances predictability of the system behavior as well.

• Tunnel and Underground Space
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• v.14 no.5
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• pp.327-338
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• 2004

The purpose of this research is to establish a concept of the factor of safety of tunnels which is a quantitative estimate of the stability of tunnels. Based on this concept, a numerical technique which calculates the factor of safety of tunnels was developed. To obtain the safety factor of a tunnel, the strength reduction technique in which a series of analyses are repeated with reduced ground strength until the tunnel collapses were employed. With this technique, the failure plane, as well as the factor of safety, can be obtained without prescribing the trial failure plane. Analyses were conducted with FLA $C^{2D}$(ver3.3), a geotechnical analysis program which is based on the finite difference method. From the result, the location of plastic zones, the maximum convergence and the maximum stress generated in the support system were also analyzed. The result shows that factors of safety are higher for the 1st and 2nd rock classes, and lower for the lower rock classes. Furthermore, factor of safety is higher when $K_{0}$ =0.5 compared to at in case of $K_{0}$ =2.0. Through this research, it is found that the factor of safety defined in this research can be used as a good quantitative index representing the stability of tunnels. Also, close examination of the results can help adjustment of the quantity and location of additional supports.s.

• International Journal of Highway Engineering
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• v.6 no.4 s.22
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• pp.75-89
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• 2004

Long term performance of concrete pavement significantly depends on the given construction and environmental condition. It means that random cracks and extreme crack width due to inappropriate quality control at the early age might lead to decreasing the pavement service life. The temperature and moisture during the construction, cement and aggregate types, curing condition are major components to affect the quality of the concrete pavement at the early age. First of all, the high temperature differential, that is made by increasing air temperature and the heat of cement hydration, is known as the major contributor to severe cracks. In this study, tent covering was used for controlling temperature of the concrete slab. The field measurement data indicates that the effect of the tent covering is very significant to decrease possibilities of random crack occurrence and curling stress and enhance the long-term concrete strength. HIPERPAV(High PERformance PAVing software), a program predicting the strength and stress of an earty-age concrete pavement (72 hour after placement), is used for simulating the effects of tent covering. The HIPERPAVE results showed that the section with the tent covering has higher reliability than the section without the tent covering by 22.5%. In details, reliability is increased 72.5% (without the tent covering) to 95% (with the tent covering).

• International Journal of Highway Engineering
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• v.13 no.4
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• pp.103-115
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• 2011

Concrete slab curls and warps due to the uneven distribution of temperature and moisture and as the result, internal stress develops within the slab. Therefore, environmental loads must be considered in addition to the traffic loads to predict the lifespan of the concrete pavement more accurately. The strength of the concrete slab is gradually decreases to a certain level at which fatigue cracking is generated by the repetitive traffic and environmental loadings. In this study, a new fatigue regression model was developed based on the results from previously performed studies. To verify the model, another laboratory flexural fatigue test program which was not used in the model development, was conducted and compared with the predictions of other existing models. Each fatigue model was applied to analysis logic of cumulative fatigue damage of concrete pavement developed in the study. The sensitivity of cumulative fatigue damage calculated by each model was analyzed for the design factors such as slab thickness, joint spacing, complex modulus of subgrade reaction and the load transfer at joints. As the result, the model developed in this study could reflect environmental loading more reasonably by improving other existing models which consider R, minimum/maximum stress ratio.

• Nuclear Engineering and Technology
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• v.13 no.4
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• pp.264-276
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• 1981

A simulation procedure which can represent time-dependent nuclear characteristics of TRIGA Mark-III reactor is developed. CITATION, a multi-group diffusion-depletion program, has been utilized as calculational tool. The group structure employed in this study consists of 7 groups: -3-fast and 4-thermal-which is conventionally utilized in TRIGA type reactor analysis. Three-dimensional nuclear characteristics are synthesized by combining results from two-dimensional plane calculation and two-dimensional cylinder calculation, since direct three-dimensional approach is not yet possible. An effort ia made to develope a method which can extract effective zone and group dependent bucklings by neutron diffusion theory rather than conventional zone and/or group independent Ducklings by neutron transport theory, since neutron leakage is quite high for small core such as research reactors. It is turned out that the method developed in this study gives satisfactory results. The calculation is performed under assumptions that all control rods are fully withdrawn, that no samples are inserted in the irradiation holes and that the core is located in the center of the reactor pool. Burnup-dependent variation of core excess reactivity, time dependent change of Xe-135 poisoning and reactivity worth of rotary specimen rack are calculated and compared with operation records. Neutron flux and power distribution as well as neutron spectrum in each irradiation .facility are presented.