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In Vitro Effect of 808-nm Diode Laser on Proliferation and Glycosaminoglycan Synthesis of Rabbit Articular Chondrocytes (토끼 관절 연골세포의 증식과 글리코스아미노글리칸 합성에 대한 808-nm 다이오드 레이저의 효능 평가)

  • Minar, Maruf;Hwang, Ya-won;Choi, Seok-hwa;Kim, Gonhyung
    • Journal of Veterinary Clinics
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    • v.32 no.4
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    • pp.295-300
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    • 2015
  • The aim of the study was to assess the in vitro effect of 808-nm InGaAs diode laser on rabbit articular chondrocyte proliferation and sulphated glycosaminoglycan (sGAG) synthesis in alginate bead. Previous studies revealed either positive or negative stimulatory effects of laser on different types of cells. A 808-nm InGaAs diode laser at 1.0W power output was used to irradiate the rabbit chondrocytes in alginate beads with energy densities of $31J/cm^2$ (G 1) and $62J/cm^2$ (G 2) corresponding to the experimental groups for 10 seconds and 20 seconds, respectively at 24, 48, 72 and 96 hours after seeding. Control group was left untreated. MTT assay was performed at 1 week and 2 weeks after the $1^{st}$ laser irradiation in alginate beads. sGAG synthesis in alginate beads at 1 week and 2 weeks were determined by DMMB assay. Histological evaluation for cellular distribution and sGAG deposition around the cells were performed by alcian blue stain. MTT assay revealed no positive stimulatory effect in cell proliferation in alginate bead. DMMB assay results showed significantly increased sGAG production in G 2 chondrocytes at 2 weeks. Image analysis of alcian blue stained slides also showed significantly higher percentage of positive alcian blue stain in G 2 chondrocytes. This result suggests that 808-nm InGaAs diode laser with 1.0 W power output although cannot stimulate cell proliferation it can increase the cell secretion activity and sGAG deposition in alginate beads.

Depositional Characteristics and Seasonal Change of Surface Sediment and Sedimentary Strucutre on the Doowoovi Tidal Flat, Southwestern Coast of Korea (한국 서남해안 두우리 조간대에서 표층 퇴적물 및 퇴적구조의 특성과 계절변화)

  • Baek Young Suk;Chun Seungsoo
    • The Korean Journal of Petroleum Geology
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    • v.10 no.1_2 s.11
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    • pp.10-17
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    • 2004
  • The Doowoo-ri tidal flat in the southwestern Korean coast is a typical open-coast tidal flat which has no barriers in the offshore such as barrier island and sand bars. The difference of induced wave energy with seasons is affected directly on the distribution of surface sediment and the formation of sedimentary structures because the sedimentation by wind wave is relatively much important element in this open-coast tidal flat. This open-coast tidal flat can be classified into tidal beach, intertidal flat and lower mudflat according to the pattern of geomorphology and sediment type. The intertidal flat can be again divided into 3 types: sand flat, mixed flat and mud flat based on the primary sedimentary structure and sand/mud ratio. Doowoori tidal flat shows a seasonal change in the surface sedimentary facies based on sediment composition and primary sedimentary structure. The change is closely related to the direction and magnitude of monsoon wind and also to storm frequency. In winter and spring, when northwesterly wind is most dominant and strong and also storms are common, sand-flat facies is largely distributed on the intertidal flat, whereas mud-flat facies is most dominant during summer when weak southeasterly wind is common. In the fall season, mixed-flat facies is dominant on the flat. The Doowoori intertidal flat is covered by mud sediment which is ca. 20 cm in thickness in summer season. In winter season, surface sediment is changed from mud to sand because the summer mud is mostly eroded by strong wave action. Can-core peels in the intertidal flat show that parallel laminated mud or sand/mud and climbing ripple cross-laminated sandy silt are dominant on the upper intertidal flat $(0-1.3 {\cal}km)$ during summer season. On the other hand, on lower intertidal flat $(1.7-2.3 {\cal}km)$, dominant sedimentary facies is homogeneous mud. In winter, it is changed into parallel laminated and ripple cross-laminated sand facies.

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The Effect of Increase in Length and Volume of Source in Radioactive Iodine Thyroid Uptake Rate (갑상선 섭취율 측정에서 선원의 길이와 부피 증가에 따른 영향)

  • Hwang, Dong Hun;Oh, Shin Hyun;Kim, Jung Yul;Kang, Chun Koo;Kim, Jae Sam
    • The Korean Journal of Nuclear Medicine Technology
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    • v.21 no.1
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    • pp.70-75
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    • 2017
  • Purpose Radioactive iodine thyroid uptake (RAIU) rate is an examination which determines and seeks about general functions of thyroid gland. The size of thyroid gland is normally different between each person, also patients having thyroid diseases have had a variety of size of thyroid gland compared with others. The purpose of this study will investigate about the counting rate which is effected by the geometric factors through the length and volume changes of the source in RAIU rate. Materials and Methods I-131 185 kBq ($5{\mu}Ci$) were placed in a cylindrical phantom of 0.5 cm, 1 cm, 1.5 cm, and 3 cm in diameter, respectively, and saline was added to gradually increase the length by 1 cm in the horizontal and vertical directions to give a change in volume. The source was measured 20 times for 20 seconds from a distance of 25 cm to $364.4keV{\pm}20%$ energy ROI with Captus 3000 thyroid uptake system (Capintec, NJ, USA). Results When the source was located in the transverse direction of the detector, the consequence of one-way ANOVA is that even though the length of source is increased each diameter, there is mostly no significant difference. When the source was located in the longitudinal direction and the counting rate of length 1 cm at all diameter is set to 100%, the average is 92.57% for length 2 cm, 86.1% for 3 cm, 80.69% for 4 cm, 74.82% for 5 cm, and 69.68% at 6 cm. Conclusion According to this study, it is expected that the gap of RAIU rate has been depended on the thickness of thyroid gland as well as the diameter of the beaker. We know that the change of the volume with the increase of the length of the source had less effect on the change of the counting rate. Thus, in order to reduce the error in the measurement of the counting rate with the thyroid uptake rate equipment, an accurate counting rate can be relatively measured if the counting rate which is measured is corrected by thickness or the distance between the thyroid and the thyroid uptake rate equipment is changed.

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Influences of Seleniferous Whole Crop Barley Supplement on Growth Performance and Blood Characteristics in Growing Pigs (셀레늄함유 청보리 급여가 육성돈의 생산성 및 혈액성상에 미치는 영향)

  • Lee, Sung-Hoon;Jo, Ik-Hwan;Kim, Guk-Won;HwangBo, Soon;Han, Ouk-Kyu;Song, Tae-Hwa;Park, Tae-Il;Choi, In-Bae
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.32 no.1
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    • pp.39-48
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    • 2012
  • This study was conducted to investigate effects of different levels of seleniferous whole crop barley (WCB) supplementation on performance, and blood characteristics as physiological responses in growing pigs. A total of 20 cross-bred pigs ((Landrace ${\times}$ Yorkshire) ${\times}$ Duroc) were divided into 4 treatments of 5 pigs each and experimental period lasted for 6 weeks. They were fed diets containing 0.1 (non-seleniferous WCB as controls), 0.2, 0.4, and 0.6 mg/kg levels of selenium (Se) by supplementing seleniferous WCB, and non-seleniferous or seleniferous WCB was formulated to 5% level in total ration. The diets were isonitrogenous (18% crude protein) and isocaloric (3,500 kcal/kg digestible energy) across treatments. Increasing levels of seleniferous WCB supplements did not affect feed intake and BW gain, and blood total protein concentration was (p<0.05) significantly higher for 0.2 mg/kg Se treatments than for controls. On d 14, blood albumin concentration was higher (p<0.05) for seleniferous WCB supplemented groups than for control group. Contrarily, blood glucose concentration was tended to be higher for controls than for seleniferous WCB groups. Blood total lipid concentration was significantly (p<0.05) lowered with increasing levels of seleniferous WCB. Serum glutamic-oxaloacetic transaminase and glutamic-pyruvic transaminase did not have any difference among treatments. It was tended that blood total cholesterol and triglyceride were lowered with increasing levels of seleniferous WCB. Blood Se concentration was significantly (p<0.05) increased with increasing levels of seleniferous WCB. The results indicate that Se present in seleniferous WCB had favorable effects on blood characteristics and blood Se increased by supplementing seleniferous WCB implies not only a good intestinal absorption of Se present in WCB but also the possibility of Se transfer into tissues.

Nutritive Effects of Feeding Rice Diet Mixed with Barley and/or Millet on the Growth Rate and Biological Availability of Some Nutrients by Albino rats (보리와 속(粟)의 혼식(混食)이 성장기(成長期) 백서(白鼠)에 미치는 영양효과(營養效果))

  • Ha, C.J.;Hyun, K.S.;Han, I.K.
    • Journal of Nutrition and Health
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    • v.9 no.3
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    • pp.1-7
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    • 1976
  • This study was carried out to observe the nutritive effects of feeding rice diet and rice diet mixed with barley or millet, or both on the growth rate and biological availability of some nutrients by albino rats. The experimental diets were prepared on the basis of isocaloric and isonitrogenous containing 357 kcal of energy and 12g of protein per 100g of diet. The experimental animals weighing about 66g of both sexes were fed on 7 kinds of diets such as control diet, rice (100%) diet, rice (70%)+barley (30%) diet, rice (70%)+millet (30%) diet, rice (70%)+barley (20%)+millet (10%) diet, rice (70%)+barley (15%)+millet (15%) diet, and rice (70%)+barley (10%)+millet (20%) diet for 7 weeks. The results obtained are summarized as follows; 1. The gain in body weight was higher for barley and/or millet mixed with rice diet groups than rice diet group with no statistical difference. 2. Although there was no difference in the amount of food consumed by experimental groups, the food efficiency ratio was sightly higher for the miked diet groups than rice atone diet group. 3. The protein efficiency ratio was also higher for barley and millet miked with rice diet groups than rice diet group, although statistical significance was not found. 4. Apparent digestibility of protein of rice diet group was significantly (P<0.01) higher than any other diet group. Although there was no remarkable difference between mixed diet group was found, the apparent digestibility of protein tended to increase when rats were fed on the barley and millet mixed with rice diet. Apparent biological value (p<0.05) and net protein utilization (p<0.01) were also significantly higher for the groups fed mixed diet with barley and/or millet than rice diet group, and those for millet alone mixed diet were slightly lower. 5. The content of total nitrogen in the liver and of protein in serum were not significantly different among experimental groups. It may be concluded from the above results that an adequate supplementation of rice with other cereals and mixing ratio of other cereals to rice were important for the efficient utilization of protein in total diet.

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A Study on the Tian-Ren relations theory of Wangfu (왕부(王符)의 천인관계론(天人關係論) 연구)

  • Cho, Won il
    • The Journal of Korean Philosophical History
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    • no.57
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    • pp.127-150
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    • 2018
  • Although Wangfu's Tian-Ren relations theory could not be free from the influence of the religious understanding of the Yinyangwuxing theory, which was prevalent in the times of two Hans, due to the limitations of the times, it refers to human status and capabilities here and there of the "Qianfulun" and traces of attempts to enhance humans' ability to preside and attempts to dilute the powers of Tian(heaven) and God can be found. In addition, the fact that the theory shifted the focus of the administration and politics of the state from Tian(heaven) to the people can be also said to be highly worthwhile. Of course, when seen from philosophical, political, social, and economic perspectives, Wangfu's primary concern can be said to have been the stabilization of people's livelihood. The influence and contribution of Wangfu's ?Qianfulun? as such on the aspects of political and philosophical thoughts in the society at the time can be said to be comparable to those of Wangchong. Although there are the limitations of the situations of the times faced by Wangfu that made him unable to surpass the standardized paradigm of the Tian-Ren response theory, when seen from other perspectives, the fact that he severely criticized the confused social conditions at that time unlike other intellectuals in the confused situations at the end of the East Han Dynasty period can be said to be worth affirmation. The fact that Wangfu, who tried to work on the basis, the stabilization of the people's livelihood, concentrated most of his energy on overcoming the situation of national internal and external troubles such as breaking down the bad customs of operating the national administration by religious notions such as natural calamity or superstition instead of trying to solve the problems of the times from the academic and ideological aspects as with Wangchong can be also said to be valuable in itself. In addition, Wangfu naturally became to take the lead in breaking down deeply superstitious notions such as the Yinyangwuxing theory and the Chanwei theory while he was conducting studies on how practical problems could be overcome facing the situations in the turbulent period.

Detection of Forest Fire Damage from Sentinel-1 SAR Data through the Synergistic Use of Principal Component Analysis and K-means Clustering (Sentinel-1 SAR 영상을 이용한 주성분분석 및 K-means Clustering 기반 산불 탐지)

  • Lee, Jaese;Kim, Woohyeok;Im, Jungho;Kwon, Chunguen;Kim, Sungyong
    • Korean Journal of Remote Sensing
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    • v.37 no.5_3
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    • pp.1373-1387
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    • 2021
  • Forest fire poses a significant threat to the environment and society, affecting carbon cycle and surface energy balance, and resulting in socioeconomic losses. Widely used multi-spectral satellite image-based approaches for burned area detection have a problem in that they do not work under cloudy conditions. Therefore, in this study, Sentinel-1 Synthetic Aperture Radar (SAR) data from Europe Space Agency, which can be collected in all weather conditions, were used to identify forest fire damaged area based on a series of processes including Principal Component Analysis (PCA) and K-means clustering. Four forest fire cases, which occurred in Gangneung·Donghae and Goseong·Sokcho in Gangwon-do of South Korea and two areas in North Korea on April 4, 2019, were examined. The estimated burned areas were evaluated using fire reference data provided by the National Institute of Forest Science (NIFOS) for two forest fire cases in South Korea, and differenced normalized burn ratio (dNBR) for all four cases. The average accuracy using the NIFOS reference data was 86% for the Gangneung·Donghae and Goseong·Sokcho fires. Evaluation using dNBR showed an average accuracy of 84% for all four forest fire cases. It was also confirmed that the stronger the burned intensity, the higher detection the accuracy, and vice versa. Given the advantage of SAR remote sensing, the proposed statistical processing and K-means clustering-based approach can be used to quickly identify forest fire damaged area across the Korean Peninsula, where a cloud cover rate is high and small-scale forest fires frequently occur.

Evaluation of Oil Spill Detection Models by Oil Spill Distribution Characteristics and CNN Architectures Using Sentinel-1 SAR data (Sentienl-1 SAR 영상을 활용한 유류 분포특성과 CNN 구조에 따른 유류오염 탐지모델 성능 평가)

  • Park, Soyeon;Ahn, Myoung-Hwan;Li, Chenglei;Kim, Junwoo;Jeon, Hyungyun;Kim, Duk-jin
    • Korean Journal of Remote Sensing
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    • v.37 no.5_3
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    • pp.1475-1490
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    • 2021
  • Detecting oil spill area using statistical characteristics of SAR images has limitations in that classification algorithm is complicated and is greatly affected by outliers. To overcome these limitations, studies using neural networks to classify oil spills are recently investigated. However, the studies to evaluate whether the performance of model shows a consistent detection performance for various oil spill cases were insufficient. Therefore, in this study, two CNNs (Convolutional Neural Networks) with basic structures(Simple CNN and U-net) were used to discover whether there is a difference in detection performance according to the structure of CNN and distribution characteristics of oil spill. As a result, through the method proposed in this study, the Simple CNN with contracting path only detected oil spill with an F1 score of 86.24% and U-net, which has both contracting and expansive path showed an F1 score of 91.44%. Both models successfully detected oil spills, but detection performance of the U-net was higher than Simple CNN. Additionally, in order to compare the accuracy of models according to various oil spill cases, the cases were classified into four different categories according to the spatial distribution characteristics of the oil spill (presence of land near the oil spill area) and the clarity of border between oil and seawater. The Simple CNN had F1 score values of 85.71%, 87.43%, 86.50%, and 85.86% for each category, showing the maximum difference of 1.71%. In the case of U-net, the values for each category were 89.77%, 92.27%, 92.59%, and 92.66%, with the maximum difference of 2.90%. Such results indicate that neither model showed significant differences in detection performance by the characteristics of oil spill distribution. However, the difference in detection tendency was caused by the difference in the model structure and the oil spill distribution characteristics. In all four oil spill categories, the Simple CNN showed a tendency to overestimate the oil spill area and the U-net showed a tendency to underestimate it. These tendencies were emphasized when the border between oil and seawater was unclear.

Experimental Study on the Performance Improvement of Velcro Reinforcement through Internal Filling (내부충진을 통한 벨크로 보강재의 성능향상에 대한 실험적 연구)

  • Jeong, Yeong-Seok;Kwon, Minho;Kim, Jin-Sup;Nam, Gwang-Sik
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.41 no.4
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    • pp.347-355
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    • 2021
  • During the earthquake, for multi-story structure, if the first floor is soft, the deformation will concentrate on that floor causing a serious damage to the column members which might leads to the collapse of the whole structure like Piloti structure during the Pohang earthquake in Korea. According to the 2016 National Disaster Management Research Institute's "Investigation of Seismic Reinforcement and Cost Analysis of Domestic Non-seismic Buildings", the rate of seismic resistance of private reinforced concrete buildings was 38.3 %. Among them, it was reported that the seismic-resistance ratio of the two to five-story structures was less than 50 %. Accordingly, the government is trying to improve the seismic rate through support projects, but the conventional seismic reinforcement methods are still expensive, and emergency construction is difficult. Therefore, in this study, the field applicability was evaluated by improving the reinforcement method using Velcro, which was developed through the research project of the Ministry of Land, Transport and Maritime Affairs in 2014. In order to improve the performance of the Velcro reinforcement method, introducing the initial tension of Velcro using high foaming rigid urethane filling between the Velcro and concrete of the columns was applied. Additionally, an experiment was conducted to evaluate the ductility of Velcro specimen from the concrete confinement effect. As a result, the ductility of the Velcro specimen was improved compare to Normal specimen. However, the energy dissipation capacity of VELCRO2 is better than VELCRO1, yet the maximum ductility of those two specimens did not show a significant difference. Therefore, the improvement of the internal filler material is still needed to have a better maximum ductility.

A study on the change effect of emission regulation mode on vehicle emission gas (배기가스 규제 모드 변화가 차량 배기가스에 미치는 영향 연구)

  • Lee, Min-Ho;Kim, Ki-Ho;Lee, Joung-Min
    • Journal of the Korean Applied Science and Technology
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    • v.35 no.4
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    • pp.1108-1119
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    • 2018
  • As the interest on the air pollution is gradually rising at home and abroad, automotive and fuel researchers have been studied on the exhaust and greenhouse gas emission reduction from vehicles through a lot of approaches, which consist of new engine design, innovative after-treatment systems, using clean (eco-friendly alternative) fuels and fuel quality improvement. This research has brought forward two main issues : exhaust emissions (regulated and non-regulated emissions, PM particle matter) and greenhouse gases of vehicle. Exhaust emissions and greenhouse gases of automotive had many problem such as the cause of ambient pollution, health effects. In order to reduce these emissions, many countries are regulating new exhaust gas test modes. Worldwide harmonized light-duty vehicle test procedure (WLTP) for emission certification has been developed in WP.29 forum in UNECE since 2007. This test procedure was applied to domestic light duty diesel vehicles at the same time as Europe. The air pollutant emissions from light-duty vehicles are regulated by the weight per distance, which the driving cycles can affect the results. Exhaust emissions of vehicle varies substantially based on climate conditions, and driving habits. Extreme outside temperatures tend to increasing the emissions, because more fuel must be used to heat or cool the cabin. Also, high driving speeds increases the emissions because of the energy required to overcome increased drag. Compared with gradual vehicle acceleration, rapid vehicle acceleration increases the emissions. Additional devices (air-conditioner and heater) and road inclines also increases the emissions. In this study, three light-duty vehicles were tested with WLTP, NEDC, and FTP-75, which are used to regulate the emissions of light-duty vehicles, and how much emissions can be affected by different driving cycles. The emissions gas have not shown statistically meaningful difference. The maximum emission gas have been found in low speed phase of WLTP which is mainly caused by cooled engine conditions. The amount of emission gas in cooled engine condition is much different as test vehicles. It means different technical solution requires in this aspect to cope with WLTP driving cycle.