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Membrane Biofouling of Seawater Reverse Osmosis Initiated by Sporogenic Bacillus Strain

  • Lee, Jin-Wook;Ren, Xianghao;Yu, Hye-Weon;Kim, Sung-Jo;Kim, In-S.
    • Environmental Engineering Research
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    • v.15 no.3
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    • pp.141-147
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    • 2010
  • The objective of this study was to assess the biofouling characteristics of the Bacillus biofilm formed on reverse osmosis (RO) membranes. For the study, a sporogenic Bacillus sp. was isolated from the seawater intake to a RO process, with two distinct sets of experiments performed to grow the Bacillus biofilm on the RO membrane using a lab-scale crossflow membrane test unit. Two operational feds were used, 9 L sterile-filtered seawater and 109 Bacillus cells, with flow rates of 1 L/min, and a constant 800 psi-pressure and pH 7.6. From the results, the membrane with more fouling, in which the observed permeate flux decreased to 33% of its initial value, showed about 10 and 100 times greater extracellular polymeric substances and spoOA genes expressions, respectively, than the those of the less fouled membrane (flux declined to 20% of its initial value). Interestingly; however, the number of culturable Bacillus sp. in the more fouled membrane was about 10 times less than that of the less fouled membrane. This indicated that while the number of Bacillus had less relevance with respect to the extent of biofouling, the activation of the genes of interest, which is initiative of biofilm development, had a more positive effect on biofouling than the mass of an individual Bacillus bacterium.

Evaluation the Impact of Installing a Isolation Valve on Condensate System of Nuclear Power Plan (원자력발전소 복수기 수실 차단밸브 설치 영향 평가)

  • Lee, Sun-Ki
    • Journal of Industrial Convergence
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    • v.18 no.4
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    • pp.15-21
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    • 2020
  • Because there are no isolation valves in condensate system of nuclear power plants, circulating water pump was shutdown for the condenser repair. When circulating water pump was shutdown, power plant output decreased about 45%. These output decreasing can minimize by establishing isolation valves. In this paper, evaluated effect to flow conditions change of condensate system, structural integrity of system, condenser pressure of in case of establish isolation valves to condensate system. Results of the evaluation, the flow rate due to the installation of the isolation valve decreased 0.3% when the valve was fully opened and 4.5% when fully closed. In addition, it was found that the vacuum degree of the condenser decreased with decreasing flow rate, but the integrity of the system was maintained.

Morphogenetic Alterations of Alternaria alternata Exposed to Dicarboximide Fungicide, Iprodione

  • Kim, Eunji;Lee, Hye Min;Kim, Young Ho
    • The Plant Pathology Journal
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    • v.33 no.1
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    • pp.95-100
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    • 2017
  • Fungicide-resistant Alternaria alternata impede the practical control of the Alternaria diseases in crop fields. This study aimed to investigate cytological fungicide resistance mechanisms of A. alternata against dicarboximide fungicide iprodione. A. alternata isolated from cactus brown spot was cultured on potato-dextrose agar (PDA) with or without iprodione, and the fungal cultures with different growth characteristics from no, initial and full growth were observed by light and electron microscopy. Mycelia began to grow from one day after incubation (DAI) and continued to be in full growth (control-growth, Con-G) on PDA without fungicide, while on PDA with iprodione, no fungal growth (iprodione-no growth, Ipr-N) occurred for the first 3 DAI, but once the initial growth (iprodione-initial growth, Ipr-I) began at 4-5 DAI, the colonies grew and expanded continuously to be in full growth (iprodione-growth, Ipr-G), suggesting Ipr-I may be a turning moment of the morphogenetic changes resisting fungicidal toxicity. Con-G formed multicellular conidia with cell walls and septa and intact dense cytoplasm. In Ipr-N, fungal sporulation was inhibited by forming mostly undeveloped unicellular conidia with degraded and necrotic cytoplasm. However, in Ipr-I, conspicuous cellular changes occurred during sporulation by forming multicellular conidia with double layered (thickened) cell walls and accumulation of proliferated lipid bodies in the conidial cytoplasm, which may inhibit the penetration of the fungicide into conidial cells, reducing fungicide-associated toxicity, and may be utilized as energy and nutritional sources, respectively, for the further fungal growth to form mature colonies as in Ipr-G that formed multicellular conidia with cell walls and intact cytoplasm with lipid bodies as in Con-G.

Complete Genome and Calcium Carbonate Precipitation of Alkaliphilic Bacillus sp. AK13 for Self-Healing Concrete

  • Jung, Yoonhee;Kim, Wonjae;Kim, Wook;Park, Woojun
    • Journal of Microbiology and Biotechnology
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    • v.30 no.3
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    • pp.404-416
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    • 2020
  • Bacteria that are resistant to high temperatures and alkaline environments are essential for the biological repair of damaged concrete. Alkaliphilic and halotolerant Bacillus sp. AK13 was isolated from the rhizosphere of Miscanthus sacchariflorus. Unlike other tested Bacillus species, the AK13 strain grows at pH 13 and withstands 11% (w/v) NaCl. Growth of the AK13 strain at elevated pH without urea promoted calcium carbonate (CaCO3) formation. Irregular vaterite-like CaCO3 minerals that were tightly attached to cells were observed using field-emission scanning electron microscopy. Energy-dispersive X-ray spectrometry, confocal laser scanning microscopy, and X-ray diffraction analyses confirmed the presence of CaCO3 around the cell. Isotope ration mass spectrometry analysis confirmed that the majority of CO32- ions in the CaCO3 were produced by cellular respiration rather than being derived from atmospheric carbon dioxide. The minerals produced from calcium acetate-added growth medium formed smaller crystals than those formed in calcium lactate-added medium. Strain AK13 appears to heal cracks on mortar specimens when applied as a pelletized spore powder. Alkaliphilic Bacillus sp. AK13 is a promising candidate for self-healing agents in concrete.

A Study on System for Policy Promotion of Korean Nuclear Power - Risk Governance with Additional Construction of Nuclear Power Plants - (한국 원자력 분야 정책추진체계 연구 - 고리원전추가건설사례로 본 위험거버넌스 구축 측면에서 -)

  • Lee, Sang-Yun;Yoon, Hong-Joo
    • The Journal of the Korea institute of electronic communication sciences
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    • v.10 no.1
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    • pp.81-94
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    • 2015
  • The purpose of this study is to find ways to build a desirable risk governance to respond to these uncertainties like such as increased risk due to the rapid development of modern science and technology in S. Korea. This study is about model of risk communication with scientific technology for additional construction of nuclear power plants. This study analyzed risk communication with scientific technology through new explanatory models between approval opinions of scientific professional group and opposition opinions of the public with Kori nuclear power plant of S. Korea. And then This study investigated related system for policy promotion in the field of nuclear energy of the current S. Korea. Consequently, Governance to strengthen the negotiations on nuclear technology at the time of the interaction in risk communication have been identified and the future of policy direction of the Korean Standard Nuclear Power Plant was detected.

MOLTEN CORIUM DISPERSION DURING HYPOTHETICAL HIGH-PRESSURE ACCIDENTS IN A NUCLEAR POWER PLANT (원자로 노심 용융물의 고압분출 및 비산 현상에 대한 수치해석적 연구)

  • Kim, Jong-Tae;Kim, Sang-Baik;Kim, Hee-Dong;Jeong, Jae-Sik
    • 한국전산유체공학회:학술대회논문집
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    • 2009.11a
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    • pp.121-128
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    • 2009
  • During a hypothetical high-pressure accident in a nuclear power plant (NPP), molten corium can be ejected through a breach of a reactor pressure vessel (RPV) and dispersed by a following jet of a high-pressure steam in the RPV. The dispersed corium is fragmented into smaller droplets in a reactor cavity of the NPP by the steam jet and released into other compartments of the NPP by a overpressure in the cavity. The fragments of the corium transfer thermal energy to the ambient air in the containment or interact chemically with steam and generate hydrogen which may be burnt in the containment. The thermal loads from the ejected molten corium on the containment which is called direct containment heating (DCH) can threaten the integrity of the containment. DCH in a NPP containment is related to many physical phenomena such as multi-phase hydrodynamics, thermodynamics and chemical process. In the evaluation of the DCH load, the melt dispersion rates depending on the RPV pressure are the most important parameter. Mostly, DCH was evaluated by using lumped-analysis codes with some correlations obtained from experiments for the dispersion rates. In this study, MC3D code was used to evaluate the dispersion rates in the APR1400 NPP during the high-pressure accidents. MC3D is a two-phase analysis code based on Eulerian four-fields for melt jet, melt droplets, gas and water. The dispersion rates of the corium melt depending on the RPV pressure were obtained from the MC3D analyses and the values specific to the APR1400 cavity geometry were compared to a currently available correlation.

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The Effect of Agricultural Wastes on Rice Plant Growth (답토양(畓土壤)의 유기물(有機物) 시용효과(施用效果))

  • Lee, Sang-Kyu;Park, Jun-Kyu
    • Applied Biological Chemistry
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    • v.27
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    • pp.56-67
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    • 1984
  • As in many other country, the use of organic matter in Korea has long history. Farmers understand the value of organic matter as the source of plant nutrient and soil improving agent in general. Since 50 years ago, the sources of organic matter in paddy soils were compost, rice and barly straw, green manure, animal waste, fish and beancake, etc.. Application of green manures such as vetch and chinese milk vetch showed no significant effect on the yield of brown rice in paddy soil. On the other hand, the effects of compost and rice straw showed more significant on the yield of brown rice in paddy soil. Application of rice straw in rice cultivation is commonly made at different times between harvest, early spring and several weeks before transplanting. Considering the suitable paddy soil for application of rice straw under well to moderately well drained soil, the yield was pronounced more than poorly drained soil. Based on laboratory and field experimants, application of rice straw promoted the decrease of oxidation-reduction potential in well to moderately well drained soil. This results to be enhanced the release of some mineral nutrients,. such as potassium, calcium, silicon, and increase of availability of soil phosphorus. In the field experiments, results obtained from nitrogen fraction on the immobilization-mineralization of the tracer nitrogen applied in paddy soil,the amount and index of organic nitrogen incoporated in soil was more pronounced in rice straw application than control. Rice straw and its transformation products incoporated in the soil, provided the inflow of energy necessary to maintain heterotrophic microbes activities. Rice straw and its transformation products, especially soluble carbohydrate, enhanced the population of free-living heterotrophic $N_2$ - fixing microbes. Moreover, rice straw and its transformation products in paddy soil, enhanced the activities of soil enzymes such as dehydrogenase and urease.

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Biodiesel: Oil-crops and Biotechnology (바이오디젤 원료 작물 품종 개량과 생명공학기술 응용)

  • Roh, Kyung-Hee;Park, Jong-Sug
    • Applied Biological Chemistry
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    • v.50 no.3
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    • pp.137-146
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    • 2007
  • The substitution of fossil fuels with biofuels has been proposed by the European Union (EU) as part of a strategy to mitigate greenhouse gas emissions from road transport, increase security of energy supply and support the development of rural communities. Vegetable oils and their derivatives (especially methyl esters), commonly referred to as 'biodiesel', are prominent candidates as alternative diesel fuels. They have advanced from being purely experimental fuels to the initial stages of commercialization. They are technically competitive with or offer technical advantages compared to conventional diesel fuel. However, several problems remain including economics, combustions, some emissions, lube oil contamination, and low-temperature properties. Therefore, quality control of fuel-related properties of biodiesel is needed to obtain consistent engine performance by fuel users. The quality of the fuel is affected by the oil composition. Rapeseed oil has been targeted for fuel use because it produces an oil with a close-to-optimum set of fuel characteristics. In this paper we have reviewed past and current efforts, both by traditional seed-breeding methods and by genetic engineering, to modify rapeseed oil quality and yield.

Effect of LED Light Quality on Growth and Flowering of Kalanchoe (LED 광질이 칼랑코에의 생육과 개화에 미치는 영향)

  • Kim, So-Hee;Heo, You;Hwang, Ryeong-Hwan;Park, Young-Hoon;Choi, Young-Whan;Suh, Jeong-Min;Kang, Jum-Soon
    • Journal of Environmental Science International
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    • v.23 no.9
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    • pp.1573-1581
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    • 2014
  • This study was conducted to examine the effect of LED light quality and supplemental LED light on growth and flowering for potted flowering plant of Kalanchoe. 1. Plant height of Kalanchoe was enhanced under Red, regardless of treatment time. 2. Root length and stem diameter of Kalanchoe were enhanced by Red+Blue. 3. The number of internodes was not influenced by LED light quality. Length of flower stalk of Kalanchoe increased under Red+Blue, but treatment time did not result in statistically significant differences. 4. Leaf area was not influenced by LED light quality in Kalanchoe. 5. The number of flower buds and open flowers was decreased by LED light treatment, but days to flowering was reduced by Red+Blue for 4 hr after sunset in Kalanchoe. 6. Chlorophyll and anthocyanin content was not significantly affected by LED light treatment, but anthocyanin content tended to increase by Blue 4 hr after sunset. 7. Fresh and dry weight did not increased by LED light treatment in Kalanchoe.

Comparison of Nutrient Intakes, Bone Metabolism and Risk Factors of Chronic Diseases between Postmenopausal Lacto-Ovo-Vegetarian and Omnivores in Kyunggi-Do (일부 폐경기여성의 채식군과 일반식군의 영양섭취상태, 골대사 및 만성 퇴행성 질환의 위험인자에 관한 비교연구)

  • 최선혜;승정자;김미현;이숙연;송숙자
    • Korean Journal of Community Nutrition
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    • v.4 no.3
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    • pp.412-420
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    • 1999
  • The amount and type of dietary protein that effects affect bone mineral loss after menopause and different dietary patterns may influence anthropometric measurements, biochemical values, and bone metabolism. Objective : Our objective was to investigate bone metabolism and chronic disease factors in two different dietary patterns. Design: The sample consisted of 40 lacto-ove-vegetarians(LOV) and 49 omnivores aged from 47 to 85 years in good condition without any medical problems. Results : The average energy intake of LOV was 1414.0kcal and omnivores, 1403.2kcal. The difference between means was not statistically significant. The differences of means of body weight and BMI between the two groups were not significant but the systolic blood pressure of LOV group was significantly lower than omniovores(p<0.05). The values of serum cholesterol(p<0.05) and glucose(p<0.05) of LOV were signifcantly lower than that of omnivores. The serum albumin level(p<0.01) of LOV was lower than it'scounterpart but in a normal range. The intake of crude fiber(p<0.05) of LOV group was significantly higher. The urinary pH of LOV was 7.4 and that of omnivores was 6.3(p<0.001). Plant calcium(p>0.05) and plant iron(p<0.001) had positive correlations with the uninary pH but animal protein(p>0.05) and animal iron(p>0.05) had negative correlations with the urinary pH. The level of urinary biochemical marker of bone resorption, deoxypyridinoline(DPD) of LOV was significantly lower than the other group(p>0.05). The T-scores of bone mineral density for the two sites in the two groups were not significantly different. Conclusions : The DPD level and urinary pH value of LOV were more desirable for the bone metabolism that the omnivores. The diet of LOV had lower risk factors of chronic diseases than the other group.

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