• IMMUNE NETWORK
• /
• 제14권5호
• /
• pp.255-259
• /
• 2014

Physical activity could be considered one of the factors that affect the immune system status and function. To find the relation between exercise and cytokines, we examined the possible effects of an 8-week endurance training program on the serum levels of cytokines, including tumour necrosis factor-alpha (TNF-${\alpha}$) and interferon-gamma (IFN-${\gamma}$) in sedentary men. A total of 30 healthy young male volunteers were randomly divided into an endurance training group and a control group. The training group followed a specific exercise protocol (running on a treadmill for 15~30 min at 50~70% maximal heart rate) for 8 weeks and the control group did not participate in any exercise program. Venous blood samples were collected from both the groups 24 h before and 24 h and 48 h after the exercise. Repeated ANOVA was used for statistical purposes. The serum levels of TNF-${\alpha}$ and IFN-${\gamma}$ were determined by ELISA. Significant (p<0.05) and non-significant (p>0.05) decreases were observed in the serum levels of IFN-${\gamma}$ and TNF-${\alpha}$, respectively, after the 8-week endurance training program. Our findings indicated that an 8-week endurance exercise may affect the serum levels of some inflammatory cytokines, suggesting the beneficial role of this training protocol in elderly population and people with certain conditions (inflammation of the vertebrae or other inflammatory diseases).

• Molecules and Cells
• /
• 제27권5호
• /
• pp.557-562
• /
• 2009

We examined the effects of the NADPH oxidase p22phox C242T polymorphism on endurance exercise performance and oxidative DNA damage in response to acute and chronic exercises. One hundred three subjects were recruited, among which 26 healthy subjects (CC: 12, TC: 12, and TT: 2) were studied during rest, exercise at 85% $VO_2max$, and recovery before and after 8 weeks of treadmill running. Lymphocyte DNA damage increased significantly in response to exercise (p < 0.05). There were no significant differences in plasma MDA, SOD concentrations and lymphocyte DNA damage between CC genotype and T allele group, but significant endurance training differences were observed. Endurance training increased exercise time to exhaustion in both the CC genotype and T allele groups (p < 0.05) but no significant difference was found between groups. The results of the current study with young, healthy, Korean men are interpreted to mean that 1) the majority had the CC genotype of the NADPH oxidase p22phox C242T polymorphism (82.5%: CC, 15.5%: TC, 1.9%: TT), 2) acute exercise increased lymphocyte DNA damage, 3) endurance training significantly increased exercise time to exhaustion, and alleviated lymphocyte DNA damage, and 4) The NADPH oxidase p22phox C242T polymorphism, however, did not alter lymphocyte DNA damage or exercise performance at rest, immediately after exercise, or during recovery.

• Journal of Nutrition and Health
• /
• 제37권10호
• /
• pp.881-887
• /
• 2004

Elevated plasma homocysteine is an independent risk factor for the development of cardiovascular disease. Exercise is generally believed to reduce the plasma homocysteine levels and therefore, being beneficial for cardiovascular disease (CVD). However, there is a possibility that athletes undergoing strenuous training and competition which increase oxidative stress may suffer from increased plasma homocysteine levels. The purpose of this study was to investigate the influence of endurance training on the plasma concentrations of B vitamins and homocysteine in 23 male adolescent field hockey players. Data collection and blood sampling was performed during the training period and non-training period. Following the training period, significant changes in energy and vitamin B6 intakes were observed in these subjects. Plasma vitamin B2, pyridoxal phosphate (PLP) and homocysteine levels were significantly higher during the training period than non-training period, whereas no difference was observed in plasma folate and vitamin B12 levels. Positive correlation was observed between plasma folate and folic acid intakes. When energy, B vitamin intakes were adjusted there was a significant negative correlation between plasma homocysteine levels and plasma riboflavin, folate and vitamin B12 levels. In conclusion, it is suggested that athletes with oxidative stress by strenuous exercise may need B vitamins since riboflavin, folic acid and vitamin Bl2 were shown to be negatively correlated with plasma homocysteine in athletes during the training period.

• The Journal of Korean Physical Therapy
• /
• 제14권4호
• /
• pp.100-118
• /
• 2002

This study was to investigate the effect of long-term high intensity endurance training on the activation of antioxidation enzyme activity, lipid peroxidation and lipoprotein metabolism. 15 subjects were divided into, endurance exercise + antioxidation Vitamin supplement(n=5), endurance exercise(n=5), and the control(n=5) groups. The endurance exercise groups(endurance exercise + antioxidation Vitamin supplement and endurance exercise) had 12 week of endurance exercise program. The antioxidation Vitamin supplement group was taken a Vitamin C tablet with 1000mg/day and Vitamin E tablet with 671.14mg/day right after lunch. The results obtained from this study were as follows; 1. Looking at the changes of SOD, Endurance exercise+antioxidation Vitamin supplement group and endurance exercise groups showed the significantly greater decrease in the activation of SOD after 12 weeks of all-out exercise. 2. Looking at the changes of CAT, Endurance exercise+antioxidation Vitamin supplement group revealed subjects tended to increase CAT after all-out exercise although statistically non-significant. Endurance exercise+antioxidation Vitamin supplement group showed the significantly greater increase in the activation of CAT after 12 weeks treatment for all-out exercise. 3. Looking at the changes of GPX, Endurance exercise+antioxidation Vitamin supplement group revealed subjects tended to increase GPX for the rest and after all-out exercise although statistically non-significant. Endurance exercise+antioxidation Vitamin supplement group showed the significantly greater increase in the activation of GPX after 12 weeks treatment for all-out exercise. 4. The MDA change showed the significant decrease after 6 weeks, after 12 weeks for the all-out exercise of Endurance exercise + antioxidation Vitamin supplement group. 5. There was non-significant change in lipoprotein metabolism for the rest and after all-out exercise.

• 운동영양학회지
• /
• 제13권2호
• /
• pp.161-167
• /
• 2009

The purpose of this study was to investigate whether two different exercises, acute exhaustive exercise and long-term endurance exercise training could affect to the expression of Mn-SOD, HSP70, and PPAR-γ protein in myocardium. The Wistar-Kyoto rats(n=24, 4 weeks) were used and randomly divided into 3 groups; endurance exercise training group (EET, n=8), acute exhaustive exercise group (AEE, n=8) or control group (CON, n=8). EET performed treadmill exercise for 12 weeks (5 days/week, 30~60 min/day). AEE exercised treadmill running (speed increased gradually to 14-26 m/min, 60 min ±10min) until exhausted when EET finished the program. Then, all the rats were sacrificed 48 hours rest at least after the last session of their own exercise program. Hearts were isolated and then the expression of Mn-SOD, HSP70, and PPAR-γ were analyzed by western blotting. One-way repeated ANOVA was used and p value under 0.05 was considered as statistical significance. The results were followed as; the expression of Mn-SOD of AEE was decreased compared with CON. However, the expression of Mn-SOD of EET was increased compared with CON. There was significant difference between AEE and EET in the expression of Mn-SOD. The expressions of HSP70 and PPAR-γ in the both AEE and EET were significantly increased compared with CON. In conclusion, acute exhaustive exercise might induce oxidative stress wheres endurance exercise training could ameliorate the oxidative conditions by increase of Mn-SOD, HSP70, and PPAR-γ. Therefore, we suggested that endurance exercise training could enhance the complementary antioxidant system and improve to prevent apoptosis. Further, a long-term moderat aerobic exercise program might play a important role in mitochondrial biogenesis in the heart.

• 생명과학회지
• /
• 제22권5호
• /
• pp.665-670
• /
• 2012

이 연구는 자연발생고혈압쥐(SHR)의 혈압, 골격근에서의 혈관생성 및 혈관내피세포성장인자 발현(VEGF)에 미치는 지구성운동의 효과를 조사하였다. 5주령 SHR와 WKY는 무작위로 정상혈압군(WKY, Wistar-Kyoto rat, n=9), 고혈압통제군(SHR-C, SHR Control, n=9) 및 고혈압운동군(SHR-E, SHR Exercise, n=9)으로 각각 분류하였고, 지구성운동은 트레드밀을 이용하였다(12-20 m/min, 0% grade, 60 min/day, 5 days/wk, 16 wk). 수축기혈압은 지구성운동에 의해 효과적으로 감소되었다(SHR-E vs. SHR-C, $p$ <0.05). 골격근의 모세혈관밀도와 VEGF 단백발현은 고혈압통제군(SHR-C)이 정상혈압군(WKY)보다 모두 감소되었으나, 지구성운동(SHR-E)이 고혈압통제군(SHR-C)에 비해 모두 증가되었다. 이러한 결과들은 지구력운동 트레이닝이 SHR 골격근의 VEGF 단백발현의 증가를 통해 모세혈관밀도를 향상시키고, 이러한 모세혈관밀도의 향상이 SHR의 혈압상승을 억제할 수 있다는 것을 의미한다.

• Journal of Nutrition and Health
• /
• 제35권10호
• /
• pp.1031-1037
• /
• 2002

The purpose of present study was to evaluate the effect of endurance exercise training on skeletal muscle free amino acid concentrations, and differences in free amino acid concentration between soleus muscle which consists of mostly slow twitch oxidative fiber and extensor digitorum longus muscle which consists of fast twitch oxidative glycolytic fiber. Sixteen male SD rats (4 weeks old) were randomly devided into two groups, and fed a purified AIN-93M diet with or without aerobic exercise training according to the protocol (running on the treadmill at 25 m/min for 60 min, 5 days a week) for 6 weeks. Exercise-training for 6 weeks significanly reduced the commulative body weight gain (p＜0.05) and food efficiency ratio (p＜0.01) of rats. The result showing mitochondrial citrate synthase activity of soleus muscle was significantly higher in exercise-trained rats compared to the value for control animals (p＜0.01) indicates aerobic exercise-training was successfully accomplished in the trained group. No difference was found in the muscle aminogram pattern between soleus muscle and extensor digitorum longus muscle of control animals. However, free amino acid concentrations of soleus muscle were from 1.2 to 3.9 times of those found in extensor digitorum longus muscle of control rats, depending on an individual amino acid. Intermediate level of endurance exercise training for 6 weeks did not influence concentrations of most of free amino acid in soleus muscle of rats collected at an overnight fasted and rested state. In contrast, isolucine and leucine concentrations in extensor digitorum longus muscle of exercise-trained rats were significantly lower than those for control animals. These results indicate that aerobic energy metabolism had not been efficiently conducted, and thereby the utilization of BCAA for energy substrate was enhanced in fast twitch oxidative glycolytic fibers of extensor digitorum longus muscle of rats followed exercise-training protocol for 6 weeks.

• 생명과학회지
• /
• 제18권1호
• /
• pp.30-37
• /
• 2008

본 연구에서는 남자 고등부 태권도 선수들을 대상으로 하여 등장성운동과 등척성 등장성운동을 혼합하여 복합 훈련을 실시한 차이를 알아보기 위하여 등속성 근 기능 중 최대우력, 동측근력비율, 양측근력비율, 근지구력을 분석한 결과를 다음과 같은 결론으로 나타내었다. 첫째, 최대우력은 좌우측 신굴근 모두에서 등장성 운동집단에 비하여 복합 훈련집단이 유의하게 증가한 것으로 나타났다. 둘째, 동측근력비율, 근지구력은 두 집단 간에 유의한 차이는 나타나지 않았지만, 운동전후에 차이에 있어서는 두 집단 모두 유의하게 증가하였다. 셋째, 양측근력비율은 두 집단 간에 유의한 차이는 나타나지 않았지만, 운동전후에 차이에 있어서는 두 집단 모두 유의하게 감소하였다. 이러한 결과로서 태권도 선수들의 경기력 증가를 위한 주요 체력요소인 평형능력, 근 지구력의 향상을 위한 방법으로 등장성 운동과 복합훈련 중 어떠한 방법으로 실시하여도 근 기능을 향상 시킬 수 있는 운동법으로 생각된다. 그러나 등척성 운동과 등장성 운동을 복합적으로 실시하였을 경우 최대우력이 등장성운동을 실시한 집단에 비하여 유의하게 증가하는 현상으로 보면 태권도 발차기의 파워를 보다 증가시키기 위해서는 등장성 운동과 등척성을 복합적으로 실시하는 운동방법이 보다 효과적인 방법으로 생각된다.

• The Journal of Korean Physical Therapy
• /
• 제15권4호
• /
• pp.384-397
• /
• 2003

The purpose of this study was to investigate the effect of Treadmill Training on WISCI level, walking velocity, walking endurance, motor score and gait cycle of spinal cord injury patient with incomplete. Four subjects with spinal cord injury participated in this study. They took walking excercise 5 times per week for 8 weeks. One time excercise spent 30minutes. The theraputic effect was evaluated by WISCI level, walk 10 meters test, walk for 12 minutes test, motor score and gait cycle. Four subjects were examined before, after 8 week, walking training. Collected data were statistically analyzed by SPSS PC for Wilcoxon signed rank test. The results of this study are as follows; 1) In WISCI level, walking velocity, walking endurance and motor score, post - treatment score were higher compared to pre-treatment score with statistical significance(p<0.05). 2) In Rt SLS, DLSII and Lt SLS, post-treatment percentage were higher compared to pre-treatment percentage with statistical significance(p<0.05). but DLSI were not statistical significance(p>0.05). The findings suggest that spinal cord injury patients with incomplete can improve their WISCI level, walking velocity, walking endurance, motor score and gait cycle through Treadmill gait training.

• 대한물리치료과학회지
• /
• 제10권2호
• /
• pp.7-16
• /
• 2003

The purpose of this study was to investigate the effect of Treadmill Training with FES(TTF) on walking velocity, gait endurance, and energy expenditure index(EEI) of hemiplegia patients with foot drop. Two subjects with hemiplegia participated in this study. They took walking excercise 5 times per week for 8 weeks. One time excercise spent 30minutes. The theraputic effect was evaluated by how many seconds they needed to walk 10 meters, how far they could walk for 12 minutes, and how much they spent energy in walking for 12 minutes. Two cases were examined before, after 4 week, and after 8 week, walking training. The results of this study are as follows; 1) Walking velocity : Case 1 increased from 0.52m/sec before walking training to 0.83m/sec after 8 weeks. Case 2 increased from 0.58m/sec to 0.92m/sec. 2) Gait endurance : Case 1 increased from 383.23m to 625.53m. Case 2 increased from 410.19m to 693.47m. 3) EEI : For comfortable walking condition, Case 1 decreased from 0.98beats/min to 0.71beats/min, and Case 2 decreased from 0.93beats/min to 0.68beats/min. For maximum walking condition, Case 1 decreased from 0.93beats/min to 0.67beats/min, and Case 2 decreased from 0.91beats/min to 0.61beats/min. The findings suggest that hemiplegia patients can improve their walking velocity, gait endurance and energy expenditure index through TTF.