• Mycobiology
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• 제50권6호
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• pp.399-407
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• 2022

Endophytic fungi occupy inner plant tissues, which results in various interactions between the fungus and host. Studies on endophytic fungi have been conducted in Korea for over 30 years. This paper summarizes the published results of those studies. The endophytic fungi of approximately 132 plant species in Korea have been studied since the 1990s, resulting in over 118 publications. The host plants featured in these studies comprised 3 species of mosses, 34 species of woody plants, and 95 species of herbaceous plants. At the family level, the most studied plants were members of the Poaceae family, covering 18 species. Regionally, these studies were conducted throughout Korea, but over half of the studies were conducted in Gyeongsangbuk-do, Gangwon-do, and Chungcheongnam-do. Relatively few studies have been conducted in a metropolis such as Seoul. We confirmed 5 phyla, 16 classes, 49 orders, 135 families, 305 genera, and 855 taxa of endophytic fungi, excluding Incertae sedis, whose relationship with others are unknown. Most of the endophytic fungi belonged to Ascomycota (93.2%), and a few belonged to Basidiomycota (3.6%). Since the diversity of endophytic fungi differs depending on the host plant, plant tissue, and distribution region, future studies should be conducted on multiple host plants and in various regions. Future studies on endophytic fungi are expected to broaden, including genomics and taxonomic and ecological studies of secondary metabolites.

• ALGAE
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• 제19권1호
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• pp.31-37
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• 2004

Farmed kelp gametophytes were previously observed to be living endophytically in filamentous red algae. The interactions of two farmed kelp species and six red algae were examined in laboratory culture. Undaria pinnatifida (Harvey) Suringar and Laminaria religiosa Miyabe demonstrated the differing abilities of zoospores to become endophytic in four host red algae and neither kelp became endophytic in two non-filamentous red algae. There was a strong seasonal component regarding infectiousness that is associated with the changes in fron erosion in U. pinnatifida from April to June. At the same time, L. religiosa showed no significant changes in frond erosion, and there were no apparent changes in infection levels in the two species they were able infect. This study indicated clear differences between two keip species with regard to their symbiotic relationship to red algae in terms of host specificity and preference of kelp gametophytes.

• 한국균학회지
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• 제46권3호
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• pp.205-211
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• 2018

Fungal endophytes are defined as fungi inhabiting plant tissues, causing no apparent disease. As the agricultural and ecological importance of endophytes has increased, many studies have been performed on various aspects, ranging from basic identification, taxonomy, and evolution to secondary metabolites with potential for human use. In the Korean Peninsula, conifers are the most commonly found evergreen trees, with approximately 30 to 60 reported taxa. Studies on the effect of conifer-associated endophytes on the host plants are required for the preservation and conservation of coniferous forests which decline by climate change and deforestation. This review summarizes the diversity of endophytic fungi in coniferous trees of Korea and their relationship with host plants.

• 한국균학회지
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• 제46권4호
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• pp.405-414
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• 2018

제주도에 서식하는 다양한 식물의 잎에서 내생균을 분리하였다. 분리된 균주들은 형태적 특성 및 internal transcribed spacer, large subunit rDNA 영역 및 beta-tubulin rDNA 유전자의 염기서열의 계통 분석을 통해 종을 동정하였다. 그 결과 6종의 국내 미기록 내생균을 확인하였고, 확인된 종은 각각 Diaporthe goulteri, Diaporthe vaccini, Rhizosphaera pini, Valsa friesii, Xylaria primorskensis, Zalerion arboricola 이다. 확인된 6종의 미기록 내생균 균주의 형태적 특성 및 염기서열 계통분석의 결과에 대해 기술하였다.

• 식물병연구
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• 제10권4호
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• pp.285-289
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• 2004

진한 적색과 이상 발효 증상을 나타내는 피수박은 막대한 경제적 손실을 주고 있다. 본 연구는 피수박의 발생 원인을 밝히기 위하여 이상 발효와 내생 세균과의 연관성을 검토하였다. 수박의 내생 세균 밀도는 정상 수박의 경우 $2.2{\sim}37.0{\times}10^3$ cfu/g fw에 불과하였으나 피수박의 경우는 $1.26{\sim}1.75{\times}10^6$ cfu/g fw로 정상 수박에 비해 밀도가 매우 높았다. 피수박에서 분리한 56균주에서 17균주가 피수박을 유발하였다. 피수박과 관련이 있는 세균은 대부분이 Gram 음성으로 호기성인 Pseudomonas spp.와 혐기성 세균이었다. 따라서 피수박은 Gram 음성인 내생 세균의 증식에 의한 이상 발효가 원인이라고 추정된다.

• Mycobiology
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• 제44권3호
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• pp.180-186
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• 2016

Trichoderma species are able to persist on living sapwood and leaves of cacao (Theobroma cacao) in an endophytic relationship. In this research, we evaluated the ability of Trichodema asperellum introduced at the incision site in the bark for side grafting with the concentration of 4 g/10 mL, 4 g/100 mL, and 4 g/1,000 mL (suspended in water) in suppressing vascular streak dieback (VSD) incidence and promoting growth of side grafts in the field. The incidence of VSD in two local clones of cacao, MCC1 and M04, without application of T. asperellum was 71.2% and 70.1% at 21 wk after grafting, respectively. However, when the two clones were treated with a concentration of 4 g/10 mL T. asperellum, the incidence was 20.6% and 21.7%, respectively, compared to 29.1% and 20.9% at 4 g/100 mL and 18.2% and 15.6% at 4 g/1,000 mL. By comparing to the control, the treatment with the same concentrations of T. asperellum listed above, the total number of stomata in MCC1 decreased by 41.9%, 30.2%, and 14.0% and in M04 by 30.5%, 21.9%, and -2.5% (exception), respectively. Otherwise, the total area of stomata opening increased by 91.4%, 99.7%, and 28.6% in MCC1 and by 203.8%, 253.5%, and 35.9% in M04, respectively. Furthermore, the number of buds and branches treated with a mixture concentration on the the two clones increased by 90.7% and 21.7%, respectively. These data showed that the application of T. asperellum to cacao scions while grafting can decrease VSD incidence in side grafts and increase growth of grafts in addition to decreasing total number of stomata, increasing total area of opened stomata, and increasing number of buds and branches.

• 한국균학회지
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• 제43권1호
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• pp.13-19
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• 2015

낙동강 지류의 하천에 자생하는 5종류의 수생식물인 마름, 물억새, 미나리, 부들, 및 줄을 채집하였다. 수생식물의 뿌리에서 분리된 34균주의 내생균류는 ITS영역 염기 서열을 분석하였다. 분리된 모든 내생균류들에 대하여 다양성 지수와 계통분석을 수행하였다. 내생균류는 17속으로서, Acremonium속, Alternaria속, Aspergillus속, Cladosporium속, Emericellopsis속, Fusarium속, Galactomyces속, Leptosphaeria속, Microsphaeropsis속, Penicillium속, Peyronellaea속, Phoma속, Pseudeurotium속, Rhizomucor속, Talaromyces속, Trematosphaeria속, Zalerion속 등이 분리되었다. 5종류의 수생식물에서 Alternaria, Talaromyces속의 균류들이 비교적 많이 분포하고 있었다. 본 연구는 낙동강 지류의 하천 습지에 자생하는 수생식물 군락에서 공생하는 내생균류의 다양성에 대한 연구이다.

• ALGAE
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• 제24권4호
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• pp.249-256
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• 2009

The occurrence of Laminarionema elsbetiae (Ectocarpaceae, Phaeophyceae), as epi-endophyte of Rhodymenia pseudopalmata (Rhodymeniales, Rhodophyta), described from Santa Isabel, Rawson, Argentina. L. elsbetiae grows in the host tissues forming epi-endophytic relationship in the epidermal, cortical and medullar layers. Epiphytic thalli of L. elsbetiae were unbranched filaments emerging from hostis surface. Reproductive structures of L. elsbetiae on the host were absent. On the contrary, free cultured individuals formed different reproductive structures. Macrozoosporangia containing a single large motile zoospore originated from vegetative cells, they were conical to cylindrical in shape, 30-50 ${\mu}m$ in length and 18-20 ${\mu}m$ in wide. Uniseriate plurilocular zoosporangia were cylindrical shape, 40 ${\mu}m$ in length and 10-13 ${\mu}m$ in wide. Sexual fusion was not seen. In mixed cultures of L. elsbetiae with R. pseudopalmata fronds, L. elsbetiae infected the host, grew as in natural host and, formed macrosporangia between host subcortical cells. Gametophytes of L. elsbetiae were filaments with diffuse growth, branched with a branch pattern alternate or opposite. Gametangia were plurilocular, uni or biseriate and lateral. When mature they contained 2 to 6 isogametes. The presence L. elsbetiae on R. pseudopalmata could be defined as an epi-endophytic relationship. The percentage of infection of R. pseudopalmata thalli by L. elsbetiae was 34%.A25% of the infected thalli presented a low, non-symptomatic level infection, whereas a 62% and a 13% of them exhibited respectively moderate and high indexes of infection.

• Advances in nano research
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• 제13권4호
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• pp.325-339
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• 2022

Endophytes ascertain a symbiotic relationship with plants as promoters of growth, defense mechanism etc. This study is a first report to screen the endophytic population in Waltheria indica, a tropical medicinal plant. 5 bacterial and 3 fungal strains in leaves, 3 bacterial and 1 yeast species in stems were differentiated morphologically and identified by biochemical and molecular methods. The phylogenetic tree of the isolated endophytes was constructed using MEGA X. Silver nanoparticles were biosynthesized from a rare endophytic bacterium Cupriavidus metallidurans isolated from the leaf of W. indica. The formation of silver nanoparticles was confirmed by UV-Visible spectrophotometer that evidenced a strong absorption band at 408.5 nm of UV-Visible range with crystalline nature and average particle size of 16.4 nm by Particle size analyzer. The Fourier Transform Infra-Red spectrum displayed the presence of various functional groups that stabilized the nanoparticles. X-ray diffraction peaks were conferred to face centered cubic structure. Transmission Electron Microscope and Scanning Electron Microscope revealed the spherical-shaped, polycrystalline nature with the presence of elemental silver analyzed by Energy Dispersive of X-Ray spectrum. Selected area electron diffraction also confirmed the orientation of AgNPs at 111, 200, 220, 311 planes similar to X-ray diffraction analysis. The synthesized nanoparticles are evaluated for antimicrobial activity against 7 bacterial and 3 fungal pathogens. A good zone of inhibition was observed against pathogenic bacteria than fungal pathogens. Thus the study could hold a key aspect in drug discovery research and other pharmacological conducts of human clinical conditions.

• 한국약용작물학회지
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• 제13권1호
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• pp.1-5
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• 2005

적변삼은 인삼에서 흔히 볼 수 있으며, 농가에 커다란 경제적 손실을 주지만, 아직까지 주원인에 대해서는 밝혀지지 않았다. 본 연구는 적변삼의 발생원인을 밝히기 위하여 적변삼과 내생 세균과의 연관성을 검토하였다. 인삼의 내생 세균 밀도는 정상 인삼의 경우 $0.96{\sim}1.5{\times}10^2cfu/g\;fw$에 불과하였으나 적변이 심한 경우는 $0.37{\sim}5.1{\times}10^7\;cfu/g\;fw$로 정상 인삼에 비하여 밀도가 매우 높았다. 적변삼에서 분리한 31개 균주는 적변정도의 차이는 있지만 적변을 유발하였다. 적변과 관련이 있는 세균은 대부분이 그람 음성균이었다. 적변을 유발하는 세균을 세균학적 특성과 16S rDNA의 염기서열 분석에 의해 동정한 결과 Agrobacterium tumefaciens, A. rhizogenes, Burkholderia phenazinium, Ensifer adharens, Lysobacter gummosus, Microbacterium Iuteolum, M. oxydans, Pseudomonas marginalis, P. veronii, Pseudomonas sp., Rhizobium leguminosarum, R. tropica, Rhodococcus erythropolis, Rh. globerulus, Variovorax paradoxus의 세균으로 동정되었다. 따라서 인삼적변의 발생은 내생세균의 침입 및 증식에 기인한 것으로 추정된다.