Browse > Article
http://dx.doi.org/10.5423/RPD.2004.10.4.285

Relationship between the Production of Fermentational Off-flavor and Presence of Microbial Endophytes in Bloody Watermelon  

Choi, Jae-Eul (Division of Plant Resources, Chungnam National University)
Choi, Chun-Hwan (Division of Plant Resources, Chungnam National University)
Ryuk, Jln-Ah (Division of Plant Resources, Chungnam National University)
An, Gil-Hwan (Department of Food Science and Technology, Chungnam National University)
Hwang, Yong-Soo (Division of Plant Resources, Chungnam National University)
Publication Information
Research in Plant Disease / v.10, no.4, 2004 , pp. 285-289 More about this Journal
Abstract
The bloody watermelon exhibiting dark red and fermentation off-flavor results in a great economic loss. As an effort to clarify the cause of the bloody watermelon, relationship between the fermentational off-flavor and the presence of endophytic bacteria was studied. The number of endophytes was 2.2-37.0 ${\times}10^3$ cfu/g fw (fresh weight) in normal watermelons, compared to 1.26-1.75 ${\times}10^6$ cfu/g fw in bloody ones. Seventeen bacteria among 56 isolates from bloody watermelons could induce bloody watermelons. The bacteria responsible for bloody watermelons were mainly Gram negative: aerobic Pseudomonas spp and some anaerobic bacteria. The results in this study strongly suggested that the bloody watermelons were produced by abnormal fermentation and growth of endophytic Gram negative bacteria.
Keywords
Bloody watermelon; Endophytic bacteria; Fermentation, Watermenlon;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Hickey, K. D., Orolaza, H., Zewet, N., vander Zwet, T. and Momol, M. T. 1998. The presence of endophytic Erwinia amylovora bacteria in symptomless apple tissue on orchard trees. Acta Hort. 489: 453-458
2 Kirchhof, G., Eckert, B., Stoffels, M., Baldani, J. I., Reis, V. M. and Hartmann, A. 2001. Herbaspirillum frisingense sp. nov., a new nirtogen fixing bacterial species that occurs in C4-fibre plants. Intl. J. Syst. Evol. Microbiol. 51: 157-168
3 Misaghi, I. J. and Donndelinger. C. R. 1990. Endophytic bacteria in symptom free cotton plants. Phytopathology 80: 808-811   DOI
4 Nei, M. and Li, W. H. 1979. Mathematical model for syudying genetic variation in terms of restriction endonucleases. Proc. Natl. Acad. Sci. USA 76: 5269-5273   DOI   ScienceOn
5 Pratella, G. C., Mari, M., Guizzardi, M. and Folchi, A. 1993. Preliminary studies on the efficency of endophytes in the biological control of the postharvest pathogens Monilinia laxa and Rhiropus stalonifer in stone fruit. Postharvest Biol. Technol. 3: 361-368   DOI   ScienceOn
6 Shishido, M. and Chanway, C. P. 1999. Spruce growth specificity after treatment with plant growth promoting pseudomonas. Can. J. Bot. 77: 22-31   DOI
7 Sturz, A. V and Christie, B. G. 1996. Endophytic bacteria of red clover as agent of allelopathic colver maize syndromes. Soil Biol. Biochem. 28: 583-588   DOI   ScienceOn
8 Yang, C, Crowley, D. E. and Menge, J. A. 2001. 16S rDNA fingerprinting of rhizosphere bacterial communities associated with healthy and Phytophthora infected avocado roots. FEMS Microbiol. Ecol. 35: 129-136   DOI   ScienceOn
9 최재을, 차선경, 김진희, 육진아, 황용수. 2003. 이상발효 참외로부터 분리한 내생 세균의 특성 및 발효증상유기. J. Kor. Soc. Hart. Sci. 44(3): 292-296
10 Komura, Y, Tochihara, H., Fukatsu, R., Nagai, Y and Yoneyama, S. 1971. Cucumber green mottle mosaic virus (watermelonestrain) in water and it's relationship to the fruit deterioration known as 'Konyaku' disease. Ann. Phytopath. Soc. Japan 37: 34-42   DOI
11 Schaad, N. W. 1988. Laboratory guide for identification of plant pathogenic bacteria. Bacteriol. Commit. Amer. Phytopath. Soc. St. Paul, MN, USA. 164 pp
12 Massol-Deya, A. A., Odelson, D. A. Hickey, R. F. and Tiedje, J. M. 1995. Bacterial community fingerprinting of amplified 16S and 16-23S ribosomal DNA gene sequences and restriction endonuclease analysis (ARDRA). In : Molecular microbial ecology manual, ed. by A. D. L. Akkermans, J. D. van Elsas, and F. J. de Bruijn, pp. 1-8. Kluwer Academic Publishers, Dortrecht, The Netherlands
13 Klijin, N., Weerkamp, A. H. and de Vos, W. M. 1991. Identification of mesophilic lactic acid bacteria by using polymerase chain reaction amplified variable regions of 16S rRNA specific and specific DNA probes. Appl. Environ, Microbiol. 57: 3390-3393
14 이기운, 이봉춘, 박호철, 이용수. 1990. 한국에서 수박에 발생한 오이 녹반 모자이크 바이러스(Cucumber Green Mottle Mosaic Virus)병에 대하여. 한국식물병리학회지 6: 250-255
15 Nejad, P. and Jhonson, P. A. 2000. Endophytic bacteria induce growth promotion and wilt disease suppression in oilseed rape and tomato. Biological Control 18: 208-215   DOI   ScienceOn
16 김병수, 양동훈, 이호철. 1999.수박, 성공적 재배와 경영. 농민신문사. 322 p
17 Liu, W. T., Marsh, T. L., Cheng, H. and Forney, L. J. 1997. Characterization of microbial diversity by determining terminal restriction fragment length polymorphisms of genes encoding 16S rRNA. Appl. Environ. Microbial. 63: 4516-4522
18 Holt, J. G., Krieg, N. R., Sneath, J. P. H., Staley, A. T. and Williams, S. T. 1994. Bergey's mannual of determinative bacteriology. Williams and Wilkins Co., Baltimore, MD. USA. pp 964
19 Mclnroy, A. J. and Kloepper, J. W. 1991. Analysis of population densities and identificationof endophytic bacteria of maize and cotton in the field. Bull. SROP 14: 328-331