• Asian-Australasian Journal of Animal Sciences
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• v.28 no.11
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• pp.1649-1656
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• 2015

Gene expression profiling has offered new insights into postmortem molecular changes associated with meat quality. To acquire reliable transcript quantification, high quality RNA is required. The objective of this study was to analyze integrity of RNA isolated from chicken skeletal muscle (pectoralis major) and its capability of serving as the template in quantitative real-time polymerase chain reaction (qPCR) as a function of postmortem intervals representing the end-points of evisceration, carcass chilling and aging stages in chicken abattoirs. Chicken breast muscle was dissected from the carcasses (n = 6) immediately after evisceration, and one-third of each sample was instantly snap-frozen and labeled as 20 min postmortem. The remaining muscle was stored on ice until the next rounds of sample collection (1.5 h and 6 h postmortem). The delayed postmortem duration did not significantly affect $A_{260}/A_{280}$ and $A_{260}/A_{230}$ ($p{\geq}0.05$), suggesting no altered purity of total RNA. Apart from a slight decrease in the 28s:18s ribosomal RNA ratio in 1.5 h samples (p<0.05), the value was not statistically different between 20 min and 6 h samples ($p{\geq}0.05$), indicating intact total RNA up to 6 h. Abundance of reference genes encoding beta-actin (ACTB), glyceraldehyde 3-phosphate dehydrogenase (GAPDH), hypoxanthine-guanine phosphoribosyltransferase (HPRT), peptidylprolylisomerase A (PPIA) and TATA box-binding protein (TBP) as well as meat-quality associated genes (insulin-like growth factor 1 (IGF1), pyruvate dehydrogenase kinase isozyme 4 (PDK4), and peroxisome proliferator-activated receptor delta (PPARD) were investigated using qPCR. Transcript abundances of ACTB, GAPDH, HPRT, and PPIA were significantly different among all postmortem time points (p<0.05). Transcript levels of PDK4 and PPARD were significantly reduced in the 6 h samples (p<0.05). The findings suggest an adverse effect of a prolonged postmortem duration on reliability of transcript quantification in chicken skeletal muscle. For the best RNA quality, chicken skeletal muscle should be immediately collected after evisceration or within 20 min postmortem, and rapidly preserved by deep freezing.

• Proceedings of the Korea Contents Association Conference
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• 2007.11a
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• pp.289-292
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• 2007

This research focuses on the relationship between participation characteristics on exercise needs of swimming participants. To this end, our research efforts questioned adult swimmers with increasing partner, time, determined factor of participation evinced higher levels of exercise needs. The research targeted the current(2006) membership of an aquatic facility operating in the Busan Gwangyeok-Shi, both male and female were selected as 299 participants of the study. This research utilized a exercise needs survey developed by Byung Gi Lee's team(1989). The data was analyzed through Reliability, Frequency and the ANOVA method. The results of this study are as follows: First, Those who regularly swim with a partners and those who swim alone showed differences in their exhibitionist tendency levels. Second, Those who scheduled their swimming activities in the evenings showed more motivation to exercise than those who swim in the mornings or afternoons. Third, Determining factors in beginning an aquatic exercise routine were quality of facilities or instructors, and these participants showed a different level of desire to reduce stress and exhibitionist tendency levels.

• Journal of Korean Academy of Nursing
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• v.21 no.1
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• pp.50-62
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• 1991

The purposes of this study were to identify causal factors of Anxiety and to analyze correlation between perceived family support and anxiety in hemiplegic patients. The subjects of this study were 88 hospitalized hemiplegic patients at Kyung - Hee Oriental medicine Hospital. Data were collected by using interview with questionaire from Jan. 15 to Mar, 31, 1990. The measurement tools used by this researcher were Kang's family support scale, Spielberger's trait anxiety scale and the other anxiety scale which was developed by this researcher, approved it's reliability and validity. For the purposes of the study, the collected data were analyzed by frequency, t-test, ANOVA and the hypothesis was tested by pearson correlation, partial correlation. The results of the study were as follow. 1) The analysis of causal factor of anxiety higher anxiety these three cases ; \circled1 1st causal factor was the delayed recovery of paralyzed upper limbs(2.42$\pm$1.27). \circled2 End causal factor was the dysfunction of physical ability(2.30$\pm$1.29). \circled3 3rd causal factor was difficulty for walking(2.30$\pm$0.83) and the anxiety level(2.02$\pm$0.83) of physical factor was the highest level than any other factors. 2) The hypothesis that the more perceived family support level is the less anxiety level of patient was supported(r＝-.29, p＝.003). 3) The analysis of the general characteristics exerting influences on anxiety level patients ; (sex, age, marrital status, religious, education level, occupation, economic status, experience of hospitalization, care giver, the period of hospitalizatiot side of paralysis). In this analysis, any factor has not a statistical significance.(p＞.05). 4) The analysis of the relationship about effective factors of family support level by the general characteristics of patient (sex, age, marrital status, religious, education level, occupation, economic status, experience of hospitalization, care giver, the period of hospitalization, side of paralysis). In this analysis, there was significant difference on perceived family support between married and bereaved patients(t＝-2.68, p＝ .009) As a result of this study, anxiety level of physical factor was higher than any other factors (psychological factor, social factor) and the delayed recovery of paralyzed upper limbs is the largest causal factor of anxiety of physical factor. Meanwhile, the relationship between the degree of family support and the level of anxiety was negatively correlated but the degree of relationship was low. Therefore, one can infer from this study that sufficient information about recovery of physical problems and family support were effective in preventing and reducing anxiety in hemiplegic patient.

• Fire Science and Engineering
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• v.31 no.2
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• pp.52-60
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• 2017

Numerical simulations were performed to evaluate the habitability of an operator for a cabinet fire in the main control room of a nuclear power plant presented in NUREG-1934. To this end, a Fire Dynamics Simulator (FDS), as a representative fire model, was used. As the criteria for determining the habitability of operator, toxic products, such as CO, were also considered, as well as radiative heat flux, upper layer temperature, smoke layer height, and optical density of smoke. As a result, the probabilities of exceeding the criteria for habitability were evaluated through the sensitivity analysis of the major input parameters and the uncertainty analysis of fire model for various fire scenarios, based on V&V (Verification and Validation). Sensitivity analyses of the maximum heat release rate, CO and soot yields, showed that the habitable time and the limit criterion, which determined the habitability, could be changed. The present methodology will be a realistic alternative to enhancing the reliability for a habitability evaluation in the main control room using uncertain information of cabinet fires.

• Proceedings of the Korean Vacuum Society Conference
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• 2012.02a
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• pp.433-433
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• 2012

As the requirement in patterning geometry continuously shrinks down, the termination of etch process at the exact time became crucial for the success in nano patterning technology. By virtue of real-time optical emission spectroscopy (OES), etch end point detection (EPD) technique continuously develops; however, it also faced with difficulty in low open ratio etching, typically in self aligned contact (SAC) and one cylinder contact (OCS), because of very small amount of optical emission from by-product gas species in the bulk plasma glow discharge. In developing etching process, one may observe that coupon test is being performed. It consumes costs and time for preparing the patterned sample wafers every test in priority, so the coupon wafer test instead of the whole patterned wafer is beneficial for testing and developing etch process condition. We also can observe that etch open area is varied with the number of coupons on a dummy wafer. However, this can be a misleading in OES study. If the coupon wafer test are monitored using OES, we can conjecture the endpoint by experienced method, but considering by data, the materials for residual area by being etched open area are needed to consider. In this research, we compare and analysis the OES data for coupon wafer test results for monitoring about the conditions that the areas except the patterns on the coupon wafers for real-time process monitoring. In this research, we compared two cases, first one is etching the coupon wafers attached on the carrier wafer that is covered by the photoresist, and other case is etching the coupon wafers on the chuck. For comparing the emission intensity, we chose the four chemical species (SiF2, N2, CO, CN), and for comparing the etched profile, measured by scanning electron microscope (SEM). In addition, we adopted the Dynamic Time Warping (DTW) algorithm for analyzing the chose OES data patterns, and analysis the covariance and coefficient for statistical method. After the result, coupon wafers are over-etched for without carrier wafer groups, while with carrier wafer groups are under-etched. And the CN emission intensity has significant difference compare with OES raw data. Based on these results, it necessary to reasonable analysis of the OES data to adopt the pre-data processing and algorithms, and the result will influence the reliability for relation of coupon wafer test and whole wafer test.

• Journal of The Korean Association For Science Education
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• v.36 no.3
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• pp.389-397
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• 2016

This study aims to test the efficacy of English-based automated computer scoring models and machine translation to score Korean college students' written responses on natural selection concept items. To this end, I collected 128 pre-service biology teachers' written responses on four-item instrument (total 512 written responses). The machine translation software (i.e., Google Translate) translated both original responses and spell-corrected responses. The presence/absence of five scientific ideas and three $na{\ddot{i}}ve$ ideas in both translated responses were judged by the automated computer scoring models (i.e., EvoGrader). The computer-scored results (4096 predictions) were compared with expert-scored results. The results illustrated that no significant differences in both average scores and statistical results using average scores was found between the computer-scored result and experts-scored result. The Pearson correlation coefficients of composite scores for each student between computer scoring and experts scoring were 0.848 for scientific ideas and 0.776 for $na{\ddot{i}}ve$ ideas. The inter-rater reliability indices (Cohen kappa) between computer scoring and experts scoring for linguistically simple concepts (e.g., variation, competition, and limited resources) were over 0.8. These findings reveal that the English-based automated computer scoring models and machine translation can be a promising method in scoring Korean college students' written responses on natural selection concept items.

• Journal of Bio-Environment Control
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• v.25 no.3
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• pp.193-199
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• 2016

The research discussed experimental results on slip resistance force of rafter-purlin connection of plastic greenhouse and suggested a test method for evaluating slip resistance performance of the connection. Slip resistance forces were measured by four kinds of constraint conditions for specimens, for example, fixing or rolling the end of specimen. According to tests, it is noticed that constraint condition is able to significantly affect the slip resistance force. From a post-hoc comparison result, slip resistance force of test under fixing ends condition was larger than those under the other three constraint conditions. But the slip resistance force results in the fixing ends condition had greater measure of dispersion and three times of standard deviation than those from other constraint conditions. Based on the results obtained, effect of constraint condition for specimen on the slip test should be studied to enhance reliability of evaluation test of the slip resistance performance.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.9 no.4
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• pp.145-152
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• 2009

Domestic Kori-1 MCR was partially modified in 2007 and will be renovated entirely in 2013. Digital devices partially replacing original analog devices have been introduced and standard alone computer systems such as SPDS have been integrated into the plant computer. Upgrading KSNP's MCR based on the ditalization is planned for 2015. However, the site engineers and operators are reluctant to the advanced systems. Therefore, a prototype for the KSNP's advanced MCR has been developed to increase the acceptance level of the operators and field engineers and also, to evaluate user interfaces and I&C architecture. For enhancing support of the operators' work, a P&ID based display system composed of multi-layers, which are linked through a context sensitive menu each other, has been adopted. The $1^{st}$ layer displays a simplified P&ID, the $2^{nd}$ layer control related diagrams such as controllers and logic diagrams, the $3^{rd}$ layer trends, etc. The end point view of MCR for KSNP is also suggested considering reliability and operability of the digital systems. Additionally, modernization strategies over the overhaul periods, that do not have much impact on operation and configuration efforts are suggested.

• Journal of Bio-Environment Control
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• v.21 no.4
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• pp.459-465
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• 2012

This study analyzed the effect of semi-rigid rafter-purlin cross-linking connection and driven steel pipe base on the static behavior of plastic greenhouse (PG). To promote the time and cost efficiency of the assembly process, each cross-linking connections of space arch type grid that consists of rafter and purlin is linked with steel-wire buckles, and each end of the rafters was driven directly to the ground to support the PG structure. However, in the design process, cross-linking connections and bases are idealized by being categorized as fully rigid or frictionless pinned, which does not appropriately reflect actual conditions. This study takes a full-scale loading test of PG and analyzes the effect of member cross-linking connections and driven steel pipe base on the behavior of a structure. The analysis provided a basis for determining the rigidity factor of member cross-linking nodes needed for finite element analysis, and the reliability of the result regarding the static behavior of PG.

• Journal of the Korean Applied Science and Technology
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• v.37 no.4
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• pp.958-968
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• 2020

This study attempted to investigate the value consciousness of purchasing behavior and purchasing intentions of personal beauty devices for women undergoing self-behavioral therapy. To this end, a survey was conducted, and a total of 342 copies of data were analyzed. As a result, In terms of the value consciousness, purchase behavior, and repurchase intention of the individual's personal beauty device, the value consciousness was the most materialistic, and the purchase behavior was the most economical. The overall repurchase intention was M=3.73 and SD=0.80. In correlations among value consciousness, purchase behavior and repurchase intention, positive correlations were confirmed (p<0.01). In terms of the influence of value consciousness on purchase behavior (aesthetics, conformity, effectiveness), 'aesthetic-pursuit factor' was the largest. In the effects of value consciousness on the economic aspect of purchase behavior, 'materialistic factor' was the highest. Lastly, in the influence of value consciousness on reliability, 'self-esteem factor' revealed the greatest effect. Furthermore, self-esteem factors had the biggest influence on repurchase intention. The above results confirmed that value consciousness of women in self-beauty care have a positive influence on their purchase behavior and repurchase intention for personal beauty devices. It is anticipated that they would be available as important marketing data in beauty device market.