• Title/Summary/Keyword: Enamel caries

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An experimental study on the effect of children's range beverage on bovine enamel (시판중인 어린이음료가 치아 표면의 탈회에 미치는 영향)

  • Baek, Hye-Jin;Kang, Kyung-Hee;Kim, Ji-Hwa
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.10 no.9
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    • pp.2523-2529
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    • 2009
  • The purpose of this study was to observe the demineralization effects of children's range beverage on bovine enamel. 15 bovine teeth without dental caries were divided into 4 experimental groups (n=12) and control group (n=3). All samples immersed in beverages maintained 48 hours by $37^{\circ}C$ in incubate respectively. The surface microhardness values(${\Delta}VHN$) between before and after 48 hours treatment decreased and DIGNOdent value increased because enamel surface were corroded by experimentation beverage. Especially, there was statistical significantly difference in the carbonated drink. Morphological changes by scanning electron microscope were indicated that children's range beverage cause significant enamel demineralization effects on bovine tooth surface among five beverages.

The remineralization effect of topical fluoride agents using confocal laser scanning microscope on artificial enamel caries aspects of convergence observation (공초점 레이저 주사 현미경을 이용한 우치 인공우식법랑질에서 불소도포 전·후의 재광화 효과에 대한 융합적 관찰)

  • Lee, Min-Sun;Kim, Hyo-Jin
    • Journal of the Korea Convergence Society
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    • v.8 no.6
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    • pp.139-145
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    • 2017
  • The aim of this study was to measure the remineralization effect of APF gel and fluride varnish on artificial enamel caries using CLSM in vitro. The samples were divided into 3 groups: control, 1.23% APF gel, 5% NaF varnish. The specimen surfaces were observed by CLSM and measured average fluorescence of the lesion(AFL). The results were analyzed using one-way ANOVA and Pearson's correlation analysis at a significance level off 0.05(PSWA 18.0, SPSS Inc., USA). There were significant differences between AFL at baseline and 1 day after fluoride application(p<0.05) but there are no significant differences between ${\Delta}$ AFL of all groups (p=0.222). Result of Pearson's correlation analysis, there are no significant correlation between VHN and AFL, but there were significant correlation between AFL at baseline and 1 day after fluoride application(r=0.811, p<0.001). Although AFL decreased after fluoride application, but there was no difference between the groups. In the future, it is necessary to test the oral environment model or in situ experiment supplemented the limitations of this study.

THE REMINERALIZATION ASPECT OF ENAMEL ACCORDING TO CHANGE OF THE DEGREE OF SATURATION OF THE ORGANIC ACID BUFFERING SOLUTION IN pH 5.5 (pH 5.5에서 유기산 완충용액의 포화도 변화에 따른 법랑질의 재광화 양상)

  • Park, Jin-Sung;Park, Sung-Ho;Park, Jeong-Won;Lee, Chan-Young
    • Restorative Dentistry and Endodontics
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    • v.35 no.2
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    • pp.96-105
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    • 2010
  • The purpose of this study is to observe and compare the remineralization tendencies of artificial enamel caries lesion by remineralization solutions of different degree of saturations at pH 5.5, using a polarizing microscope and computer programs (Photoshop, Image pro plus, Scion Image, Excel). For this study, 48 sound permanent teeth with no signs of demineralization, cracks, or dental restorations were used. The specimens were immersed in lactic acid demineralization solution for 2 days in order to produce artificial dental caries that consist of surface and subsurface lesions. Each of 9 or 10 specimens was immersed in pH 5.5 lactic acid buffering remineralization solution of four different degrees of saturation (0.507, 0.394, 0.301, and 0.251) for 12 days. After the demineralization and remineralization, images were taken by a polarizing microscope (${\times}100$). The results were obtained by observing images of the specimens, and using computer programs, the density of caries lesions were est mated. While the group with the lowest degree of saturation (0.251) showed total remineralization feature from the surface to the subsurface of the lesion, the group with the highest degree of saturation (0.507) showed demineralization mainly on the surface of the lesion at the constant organic acid concentration 0.01 M and pH 5.5.

THE EFFECT OF LACTIC ACID CONCENTRATION AND pH OF LACTIC ACID BUFFER SOLUTIONS ON ENAMEL REMINERALIZATION (완충 용액의 유산 농도와 pH가 법랑질의 재광화에 미치는 영향)

  • Kwon, Jung-Won;Suh, Duk-Gyu;Song, Yun-Jung;Lee, Yun;Lee, Chan-Young
    • Restorative Dentistry and Endodontics
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    • v.33 no.6
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    • pp.507-517
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    • 2008
  • There are considerable in vitro and in vivo evidences for remineralization and demineralization occurring simultaneously in incipient enamel caries. In order to "heal" the incipient dental caries, many experiments have been carried out to determine the optimal conditions for remineralization. It was shown that remineralization is affected by different pH, lactic acid concentrations, chemical composition of the enamel, fluoride concentrations, etc. Eighty specimens from sound permanent teeth without demineralization or cracks, 0.15 mm in thickness, were immersed in lactic acid buffered demineralization solutions for 3 days. Dental caries with a surface zone and subsurface lesion were artificially produced. Groups of 10 specimens were immersed for 10 or 12 days in lactic acid buffered remineralization solutions consisting of pH 4.3 or pH 6.0, and 100, 50, 25, or 10 mM lactic acid. After demineralization and remineralization, images were taken by polarizing microscopy (${\times}100$) and micro-computed tomography. The results were obtained by observing images of the specimens and the density of the caries lesions was determined. 1.As the lactic acid concentration of the remineralization solutions with pH 4.3 was higher, the surface zone of the carious enamel increased and an isotropic zone of the subsurface lesion was found. However, the total decalcification depth increased at the same time. 2.In the remineralization solutions with pH 6.0, only the surface zone increased slightly but there was no significant change in the total decalcification depth and subsurface zone. In the lactic acid buffer solutions with the lower pH and higher lactic acid concentration, there were dynamic changes at the deep area of the dental carious lesion.

Oral findings and dental management of a patient with Moebius syndrome: a case report

  • Lee, Eunkyoung;Kim, Youngjin;Kim, Hyunjung;Nam, Soonhyeun
    • The Journal of Korea Assosiation for Disability and Oral Health
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    • v.10 no.2
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    • pp.101-105
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    • 2014
  • Moebius syndrome is a rare, congenital neurological disease involving facial paralysis and limitation of eye movements. It results from maldevelopment of the sixth and seventh cranial nerves. Dental features of this syndrome include micrognathia, microstomia, tongue deformity, cleft palate, hypoplasia of the teeth, and congenital missing teeth. A 7-year-old female with Moebius syndrome was referred from a local dental clinic for caries treatment. She presented with facial paralysis and microstomia. Oral findings included multiple caries with enamel hypoplasia, congenital missing teeth, and tongue deformity. Dental treatments including restorative and preventive procedures were performed. Oral findings and management aspects of Moebius syndrome for this case are discussed. Early evaluation and multidisciplinary care are needed for children with Moebius syndrome.

Effects of Pulsed Nd:YAG Laser Irradiation and Fluoride Compound Applicatin on Acid Resistance of Bovine Teeth (Pulsed Nd:YAG 레이저 조사와 불소화합물 도포가 치아 내산성에 미치는 영향)

  • An-Hee Lee;Woo-Cheon Kee
    • Journal of Oral Medicine and Pain
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    • v.20 no.2
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    • pp.429-447
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    • 1995
  • This study was designed to determine the most effective concentration of fluoride and levels of laser irradiation for the remineralization of decayed teeth. After irradiation with a pulsed Nd:YAG laser and the topical application of fluoride, phosphate and fluoride concentration in enamel were measured. And then the changes on surface enamel using an scanning electron microscope were observed. Samples by extraction healthy, permanent, mandibular bovine teeth with no caries were obtained. Among them 371 healthy samples were selected and artificial carious lesions were made. 20 samples were assigned to each experimental group. After irradiation with a pulsed Nd:YAG laser with total energy densities of 10J/$\textrm{cm}^2$, 20J/$\textrm{cm}^2$ for each group. On the teeth, 2% NaF, 1.9% NH4F, 1.6% TiF4 Elmex gel(amine fluoride) and 1.23% APF gel were applied. After pH circulatory procedures, concentrations of fluoride with and Ionalyzer (Orion Research, Model 901, USA) and phosphates with an Uv/V is spectrophotometer (Uvikon 860, Kontrom Co, Switzerland) were measured. By etching the teeth in layers and measuring fluoride concentrations, a profile of fluoride penetration according to depth could be developed. And also the changes on the surface of the enamel using an electron scanning microscope were observed. The comparative analysis yielded the following results : 1. Phosphate concentration was low in all groups compared with the control group except for teeth treated Elmex gel, irradiated with 10J/$\textrm{cm}^2$ and 30J/$\textrm{cm}^2$ energy densities. Teeth treated with APF gel and 30J/$\textrm{cm}^2$ irradiation gad the lowest phosphate concentration. 2. Among all groups, fluoride concentrations in tooth enamel were highest in APF gel and NaF groups irradiated at 30J/$\textrm{cm}^2$. The APF gel group had the highest fluoride concentrations across all energy densities. 3. In the APF gel group, and the NaF group, the greater the energy density of the laser, the higher the fluoride concentrations in the enamel. 4. In all groups, the concentration of fluoride in the enamel by depth tended to decrease with depth. 5. Under the scanning electron microscope, under the condition of irradiation with 20J/$\textrm{cm}^2$, enamel crack was detected for the first time. In the NH4F group, spherical deposits were found on the surface of the enamel, and in the TiF4 group the surface of enamel was covered with an irregular, thin membranous mass in places. In the APF gel and NaF groups irradiated with 10J/$\textrm{cm}^2$, spherical and irregular particles covered the teeth. When these groups were irradiated at 20J/cm2, they were covered with amorphous crystals. These results suggest that one could obtain more effective anticariogenic effects without damage to teeth when less than 20J/$\textrm{cm}^2$ energy densities and APF gel are used.

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AN EXPERIMENTAL STUDY ON THE ANTICARIOGENIC EFFECT OF FLUORIDE-RELEASING PIT AND FISSURE SEALANT (불소방출 치면열구전색제의 항우식효과에 관한 실험적 연구)

  • Kim, Jung-Wook
    • Journal of the korean academy of Pediatric Dentistry
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    • v.25 no.4
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    • pp.849-857
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    • 1998
  • The purpose of this study was to investigate whether fluoride-releasing pit and fissure sealant was more effective in preventing caries than conventional non-fluoride-releasing sealant. Specimens 8mm in diameter were made from sound bovine enamel. Fluoride-releasing pit and fissure sealant(Helioseal F, Vivadent, Schaan, Liechtenstein) and non-fluoride-releasing sealant(Helioseal, Vivadent, Schaan, Liechtenstein) were applied to the specimens and artificial caries was induced. Microhardness and the depth of the carious lesion was measured. The following results were obtained: 1. In group 2, sealed with fluoride-releasing sealant, there was a 58.4% decrease in microhardness. This was significantly less than the 84.4% decrease observed in group 1, sealed with non-fluoride-releasing sealant(p<0.01). 2. The average depth of the artificial carious lesion in group 2 was $30.1{\pm}9.8{\mu}m$. In group 1, sealed with non-fluoride-releasing sealant, the lesion was significantly deeper with an average depth of $58.5{\pm}4.9{\mu}m$(p<0.01). 3. Fluoride-releasing pit and fissure sealant was more anticariogenic compared to non-fluoride-releasing sealant.

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Evaluation of Caries Status among Adolescents in Jeonju City with WHO Basic Methods, International Caries Detection and Assessment System II (ICDAS-II) (WHO basic methods와 International Caries Detection and Assessment System II (ICDAS-II)를 사용한 전주시 청소년의 우식상태 조사)

  • Park, Kibong;Kim, Doyoung;Lee, Daewoo;Kim, Jaehwan;Yang, Yoenmi;Kim, Jaegon
    • Journal of the korean academy of Pediatric Dentistry
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    • v.43 no.4
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    • pp.382-390
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    • 2016
  • Since it is favorable to include the incipient caries lesion in the diagnostic criteria in adolescence, this study had surveyed dental caries status of adolescents at ages of 13 and 16 by using WHO basic methods and ICDAS-II codes. In this study, mean DMFT index was 3.71, and mean DT index was 1.94. For both indices, the age 16 group showed higher values than the age 13 group. By groups of teeth, DMFT index and DT index exhibited highest to the lowest values in molar, premolar, and anterior teeth, respectively. 77.46% of total numbers of teeth were classified as code 0 in ICDAS-II. Compared to anterior teeth, numbers of decayed teeth were increased in posterior teeth. All caries lesions in anterior teeth and premolars were limited to enamel. ICDAS-II code is an useful method to detect the incipient caries lesion, allowing preventive control on caries management.

THE CHANGE OF THE CONFIGURATION OF HYDROXYAPATITE CRYSTALS IN ENAMEL BY CHANGES OF PH AND DEGREE OF SATURATION OF LACTIC ACID BUFFER SOLUTION (유산 완충용액의 pH 및 포화도 변화에 따른 법랑질 내 수산화인회석 결정 형태의 변화)

  • Chon, Young-Eui;Jung, Il-Young;Roh, Bung-Duk;Lee, Chan-Young
    • Restorative Dentistry and Endodontics
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    • v.32 no.6
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    • pp.498-513
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    • 2007
  • Since it was reported that incipient enamel caries can be recovered, previous studies have quantitatively evaluated that enamel artificial caries have been, remineralized with fluoride showing simultaneously the increase of width of surface layer and the decrease of width of the body of legion. There is, however, little report which showed that remineralization could occur without fluoride. In addition, the observations on the change of hydroxyapatite crystals also have been scarcely seen. In this study, enamel caries in intact premolars or molars was induced by using lactic acidulated buffering solutions over 2 days. Then decalcified specimens were remineralized by seven groups of solutions using different degree of saturation(0.212, 0.239, 0.301, 0.355) and different pH(5.0, 5.5, 6.0) over 10 days. A qualitative comparison to changes of hydroxyapatite crystals after fracturing teeth was made under SEM(scanning electron microscopy) and AFM(atomic force microscopy). The results were as follows: 1. The size of hydroxyapatite crystals in demineralized area was smaller than the normal ones. While the space among crystals was expanded, it was observed that crystals are arranged irregularly. 2. In remineralized enamel area, the enlarged crystals with various shape were observed when the crystals were fused and new small crystals in intercrystalline spaces were deposited. 3. Group 3 and 4 with higher degree of saturation at same pH showed the formation of large clusters by aggregation of small crystals from the surface layer to the lesion body than group 1 and 2 with relatively low degree of saturation at same pH did. Especially group 4 showed complete remineralization to the body of lesions. Group 5 and 6 with lower pH at similar degree of saturation showed remineralization to the body of lesions while group 7 didn't show it. Unlike in Group 3 and 4, Group 5 and 6 showed that each particle was densely distributed with clear appearance rather than crystals form clusters together.

Potential impact of metal crowns at varying distances from a carious lesion on its detection on cone-beam computed tomography scans with several protocols

  • Matheus Barros-Costa;Eduarda Helena Leandro Nascimento;Iago Filipe Correia-Dantas;Matheus L. Oliveira;Deborah Queiroz Freitas
    • Imaging Science in Dentistry
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    • v.54 no.1
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    • pp.49-56
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    • 2024
  • Purpose: This study evaluated the impact of artifacts generated by metal crowns on the detection of proximal caries lesions in teeth at various distances using cone-beam computed tomography (CBCT). Additionally, the diagnostic impacts of tube current and metal artifact reduction (MAR) were investigated. Materials and Methods: Thirty teeth were arranged within 10 phantoms, each containing 1 first premolar, 1 second premolar, and 1 second molar. A sound first molar (for the control group) or a tooth with a metal crown was placed. Of the 60 proximal surfaces evaluated, 15 were sound and 45 exhibited enamel caries. CBCT scans were acquired using an OP300 Maxio unit (Instrumentarium, Tuusula, Finland), while varying the tube current (4, 8, or 12.5 mA) and enabling or disabling MAR. Five observers assessed mesial and distal surfaces using a 5-point scale. Multi-way analysis of variance was employed for data comparison, with P<0.05 indicating statistical significance. Results: The area under the curve (AUC) varied from 0.40 to 0.60 (sensitivity: 0.28-0.45, specificity: 0.44-0.80). The diagnostic accuracy was not significantly affected by the presence of a metal crown, milliamperage, or MAR(P>0.05). However, the overall AUC and specificity were significantly lower for surfaces near a crown (P<0.05). Conclusion: CBCT-based caries detection was not influenced by the presence of a metal crown, variations in milliamperage, or MAR activation. However, the diagnostic accuracy was low and was further diminished for surfaces near a crown. Consequently, CBCT is not recommended for the detection of incipient caries lesions.