• The Journal of the Korean dental association
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• v.9 no.4
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• pp.151-157
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• 1971

Freshly extracted permant teeth were examined by electron microscope according to the Filmy replica method. Preparatory method with hydrochloric acid or proteolytic enzyme treatment was used in order to injure the structural composition of enamel surface to compare between the natural carious defects and the etched samples used in this study. The natural carious defects were chalky white or brown discolored portions at the smooth enamel surface without having visible loss of enamel substances. The findings were as follows: 1. The first caries attact initiated from the mineralized portion of rod sheath which is located at the top of enamel rod. 2. The caries developed downward along the rod sheath interprismatic substances and enamel rods subsequently. However, the caries involvement of interprismatic substances and enamel rods occured almost at the same time. 3. The rod sheath attached to the remineralized smooth emael surface was located at the top of enamel rod.

• Restorative Dentistry and Endodontics
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• v.21 no.2
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• pp.470-488
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• 1996

The end products of the metabolism of the oral microorganism, organic acids, are an element that produces dental caries. Four organic acids in plaque fluid, lactic acid, acetic acid, succinic acid, propionic acid which take the important role in producing dental caries, were chosen to evaluate the effect of acid type and concentration. The subject, $100{\mu}m$ in thickness, were immersed in acid-buffer solution which has the different acid concentration of 10mM, 25mM, 50mM, 100mM and pH 4.3 and degree of saturation was $0.153{\pm}0.003$ kept in constant and were operated to produce artificial caries under different demineralization time (1, 2, 3 day) at x25. The results were obtained by observing under polarizing microscope at x25. 1. The subsurface lesion, specific finding of incipient enamel caries, showed positive birefringence. but surface zone and sound enamel showed negative birefringence. 2. The demineralization rate of enamel was increased as the acid concentration increased. 3. The subsurface lesion showed increasing depth in the order of lactic, acetic, propionic acid, succinic acid. 4. The concentration of organic acid in artificial caries system had an independent effect on demineralization rate in enamel under the constant pH and degree of saturation. The result of this study showed that not only pH and the acid strength but the concentration of organic acid had an independent effect on demineralization rate in early enamel caries. And through the further research on the factors influencing enamel demineralization, it will be necessary to develop an effective caries preventive therapy.

• Journal of dental hygiene science
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• v.13 no.4
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• pp.461-470
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• 2013

In order to examine the anticariogenic effect after fluoride varnish was applied to sound enamel and artificial caries enamel, anterior teeth of healthy cattle were used and divided into four groups such as group 1 (sound enamel), group 2 (application of fluoride varnish to sound enamel), group 3 (artificial caries enamel) and group 4 (application of fluoride varnish to artificial caries enamel). Remineralization on the surface of enamel and changes of crystalline structure after demineralization were observed by using a field emission scanning electron microscope (FE-SEM). Quantitative analysis of Ca and P was measured by using the energy dispersive X-ray spectrophotometer (EDS). The following conclusions were obtained: 1)Surface pattern of enamel was the roughest in group 3 due to the defects caused by porosity and microcracks. Group 4, group 1 and group 2 were followed in order; 2) It was found that pattern of crystalline structures in a group of application of fluoride varnish and a group of no application showed bigger change in artificial caries enamel than that in sound enamel. In other words, groups 4 and 1 showed a similar pattern; 3) The contents of Ca and P were higher in groups of application of fluoride varnish (group 2 and group 4) than in groups of no application of fluoride varnish (group 1 and group 3). Taken results of this study together, in the case of application of fluoride varnish, crystalline structure was changed by remineralization even in the sound enamel. In particular, porous structures showed a smooth and uniform pattern due to the recalcification in the artificial caries enamel. In addition, according to results of EDS analysis, the contents of Ca and P were increased and it had great anticariogenic effects which inhibit decalcification of sound enamel and artificial caries enamel.

• Journal of Korean society of Dental Hygiene
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• v.17 no.5
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• pp.817-826
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• 2017

Objectives: The aim of this study was to investigate the effects of bamboo salts on remineralization effects on subsurface of artificial carious enamel. Methods: Incipient carious enamel was formed in permanent bovine incisors and then specimens were divided into three groups randomly: 3% bamboo salt (BS), 2% (NaF) and the solution of mixed 3% BS and 2% NaF. For remineralization, specimens of each of the three groups were treated for 24 hrs at $37^{\circ}C$ incubator. After treatment, specimens were analyzed using SEM and VHN. Statistical analysis used was one-way ANOVA. Results: In SEM observation, the BS group showed narrower distances between enamel rods than the cases of incipient subsurface caries enamel. The NaF and BS+NaF groups showed that the enamel rods near the surface were destructed, and innumerable round small particles were deposited near the surface of enamel. The BS+NaF group showed more minerals attachment between enamel rods than the cases of other groups. The differences in subsurface microhardness (${\Delta}VHN$) increased all of three groups in total by $80{\mu}m$. The ${\Delta}VHN$ of the BS+NaF group increased significantly more than NaF and BS groups in depth of $50{\mu}m$, $80{\mu}m$. Conclusions: The 3% bamboo salt with 2% NaF solution was found to increase subsurface hardness of incipient caries enamel. Thus, bamboo salt will be used to contribute to prevention on dental caries.

• Restorative Dentistry and Endodontics
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• v.27 no.2
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• pp.175-182
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• 2002

Dental caries is a chronic disease that causes the destruction of tooth structure by the interaction of plaque bacteria, food debris, and saliva. There has been attempts to induce remineralization by supersaturating the Intra-oral environment around the surface enamel, where there is incipient caries. In this study, supersaturated remineralized solution "R" was applied to specimens with incipient enamel caries, and the quantitative analysis of remineralization was evaluated using microradiography. Thirty subjects volunteered to participate in this study. Removable appliances were constructed for the subjects, and the enamel specimen with incipient caries were embedded in the appliances. The subjects wore the intra-oral appliance for 15 days except while eating and sleeping. The removable appliance were soaked in supersaturated solution "R", saline, or Senstime$^{\circledR}$ to expose the specimen to those solutions three times a day, 5 minutes each time. After 15 days, microradiography was retaken to compare and evaluate remineralization The results were as the following: 1. The ratio of remineralized area to demineralized area was significantly higher in the supersaturated solution "R" and Senstime$^{\circledR}$ than in the saline (p<0.05) 2. Remineralization in the supersaturated buffer solution "R" occurred in the significantly deeper parts of the tooth. compared to the Senstime$^{\circledR}$ group containing high concentration or fluoride. (p<0.05) As in the above results, the remineralization effect of remineralized buffer solution "R" on incipient enamel caries has been proven. For clinical utilization, further studies on soft tissue reaction and the effect on dentin and cementum are necessary In conclusion compared to commercially available fluoride solution. remineralization solution“R”showed better remineralization effect on early enamel caries lesion, so it is considered as effecient solution for clinical application.

• The Journal of the Korean dental association
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• v.49 no.8
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• pp.451-460
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• 2011

Dental caries has been widely prevalent with presence of cavitation on teeth. For the last several decades, the prevalence of dental caries in developed countries has rapidly decreased so there has been needed a new and detailed diagnostic guideline to differentiate the severity of dental caries, especially for early status of caries. The cariology specifically requires the development of an integrated definition of dental caries and uniform systems for measuring the caries process in the fields of clinical diagnosis and treatment, epidemiological researches, and dental education and so forth. The international Caries Detection and Assessment System (ICDAS) optically measures the enamel surface changes and potential histological depth of carious lesions by relying on surface characteristics of teeth. ICDAS is a visual classification system that was developed to diagnose the subtle changes of enamel surface, predict the progress direction of early caries, allow standardized data collection in relation to caries in different settings, and to enable better comparison of oral health between countries worldwide and research studies.

• Journal of the korean academy of Pediatric Dentistry
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• v.24 no.1
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• pp.313-324
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• 1997

The aim of the present study was to describe an safe and convenient method for the early detection of enamel caries using laser fluorescence. Fluorescence from natually carious lesion of human teeth illuminated by an argon laser(488nm) was observed and photographed using barrier filter. Intact enamel was found to fluorescence with a yellowish light. Whereas, incipient caries lesions in the enamel were dearly visible as dark areas in contrast to the fluorescence surroundings. For evaluation of accuracy of this method, lesion depth measured by the laser fluorescence in light microscope was compared with that polarizing microscope. The results from the present study can be summarized as follows : 1. Enamel caries of smooth surface was observed as pale white spot and undefined outline in ordinary light. Whereas, lesion was clearly visible as dark spot in laser fluorescence. 2. There was no difference between ordinary light view and laser fluorescence in occlusal surface and interproximal surface. 3. There was no significant difference between the lesion depth observed by laser fluorescence with light microscope and polarizing microscope. Apparent correlation exists between two groups.

• Proceedings of the KACD Conference
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• 2001.11a
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• pp.560.2-560
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• 2001

Dental caries is a chronic disease that causes the destruction of tooth structure by the interaction of plaque bacteria, food debris, and saliva. There has been attempts to induce remineralization by supersaturating the intra-oral environment around the surface enamel, where there is incipient caries. In this study, supersaturated remineralized solution was applied to specimens with incipient enamel caries, and the quantitative analysis of remineralization was evaluated using microradiography.(omitted)

• Restorative Dentistry and Endodontics
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• v.17 no.1
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• pp.206-213
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• 1992

The aim of this study was to compare the accuracy of radiographic diagnosis of secondary carious lesions adjacent to composite filling materials with different radiopacity. The level of radiopacity that is most compatible with the radiographic diagnosis of secondary caries was studied in a two part experiment. In the first part, the radiopacity of 6 posterior composites CBP, CF, HM, LF, PQ, P50), enamel and dentin were measured by desitometer and 6 posterior composites divided into 3 groups based on their level of radiopacity compared with enamel and dentin. In the seocnd part, class II composite fillings with or without secondary caries were made in extracted premolar and radiographs of the teeth were examined by 10 dentists to diagnose simulated carious lesion. The following results were obtained: 1. The radiopacity of 6 posterior composites varied between 1.76(PQ) and 6.78(P50)mm Al equivalent. 2. For 4 composites the radiopacity exeeded that of an equal thickness of enamel, and for two the radiopacity was lower than that of dentin. 3. The detection of secondary caries was facillitated when the radiopacity of a composite resin was similar to or slightly greater than that of enamel.

• Journal of the korean academy of Pediatric Dentistry
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• v.1 no.1
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• pp.9-17
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• 1974

The purpose of this study was to clarify the relationship between dental caries status, and the content of fluoride in drinking water, and the content of fluoride in enamel of deciduous teeth of Korean children. The specific areas selected in this study were Asan-Kun and Seouchon-Kun (Chung Cheoung Nam-Do) where the fluoride content in the deep well water were 0.9 ppm and 0.4 ppm respectively. The dental caries status of 720 children aged 6 to 11 who have been lived since birth was detected. The following results were obtained upon this study: 1. The def person rate, deft rate and def index of the Asan-Kun children were revealed 46.11%, 14.41% and 1. 69 respectively. The fluoride content in the outer layer of enamel was 430 ppm. 2. The def person rate, deft rate and def index of the Seouchon-Kun children were revealed 59.17%, 20.97% and 2.40 respectively. The fluoride content in the outer layer of enamel was 391 ppm. 3. The def person rate, deft rate and def index of the Asan-Kun children were showed lower value than the Seouchon-Kun children. The fluoride content of the outer layer of enamel of the Asna-Kun children were showed higher value than the Seouchon-Kun children.