• Journal of Korean Society of Forest Science
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• v.99 no.4
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• pp.568-572
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• 2010

In order to develop an efficient micropropagation system for a rare tree species, Empetrum nigrum var. japonicum K. Koch, the effect of medium salt, cytokinins and auxin at different concentration were evaluated. Shoot induction from axillary bud was better on WPM medium than on MS medium. Although there was no significant differences observed in shoot induction among the salt strengths of WPM medium, whereas healthy shoots were developed on basal WPM medium. In comparison of the cytokinins affecting shoot proliferation, zeatin was better than BA, whereas BA exhibited more effectiveness on shoot elongation. In vitro root formation was better on WPM medium than on 1/2MS medium and achieved the highest rooting rate when 5.0 mg/L IBA treatment. 93% of rooted plantlets were survived on artificial soil mixture after 4 weeks of acclimatization. Above results suggest that a rare tree species, E. nigrum var. japonicum can be micropropagated via axillary bud cultures.

• Korean Journal of Plant Resources
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• v.25 no.6
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• pp.739-744
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• 2012

The plant Empetrum nigrum, valued in the traditional system of medicine, is well known for its antibacterial, antifungal, and antioxidant properties. In the present work, the effect of removal of shoot apical meristem (SAM) on shoot proliferation was studied. It was observed that removal of SAM promoted shoot proliferation whereas intact tip resulted in higher survival percentage. Further, the effect of different concentrations of BA on above was also studied. During root formation the effect of light quality after treatment with IBA was investigated. For rooting, continuous red light without IBA resulted in maximum rooting percentage. The above factors when taken into consideration during micropropagation of this endangered plant can result in healthier plantlets. The results show that the species could be successfully conserved by in vitro propagation system.

• Korean Journal of Plant Resources
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• v.25 no.5
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• pp.633-639
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• 2012

This study was performed to evaluate the effect of ethanolic extract from the fruit of Empetrum nigrum var. japonicum (EN) on $CCl_4$-induced hepatotoxicity. Orally provided daily for 7 days were 250-mg/kg or 500-mg/kg EN or vehicle, while $CCl_4$ (40 mg/kg) was intraperitoneally injected the day after the last treatment of EN. Twenty-four hours after injection of $CCl_4$, we measured serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels, malondialdehyde (MDA) contents, superoxide dismutase (SOD), and catalase (CAT) activity of the liver. The antioxidant activities were measured with the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity and inhibitory effect on lipid peroxidation. The EN showed a strong DPPH radical scavenging activity and inhibitory effect of lipid peroxidation. The ALT and AST levels in serum were greatly enhanced by the $CCl_4$ injection. However, in the EN treatment group, the levels of ALT and AST in serum were significantly reduced. Moreover, $CCl_4$ significantly increased the MDA contents and decreased the SOD and CAT activity in liver homogenates. The EN recovered MDA contents, close to that in the normal group, while the EN increased the SOD and CAT activity. These results suggest that ethanolic extract from the fruit of Empetrum nigrum var. japonicum has significant antioxidant activity and hepatic protection potential.

• Proceedings of the Plant Resources Society of Korea Conference
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• v.18 no.1
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• pp.110-111
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• 2005

• Proceedings of the Plant Resources Society of Korea Conference
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• 2005.11a
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• pp.59-59
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• 2005

• Korean Journal of Plant Resources
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• v.19 no.4
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• pp.530-536
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• 2006

Hardwood cutting propagation and early growth characteristics were investigated in order to develop the method of cutting propagation and to find out growth characteristics in the low altitude for in situ and ex situ conservation of Empetrum nigrum var. japonicum K. Koch, which is typical arctic alpine plants on Mt. Halla. The growth of roots and shoots was different depending on hormone concentrations or soil conditions. The survival rate, rooting rate, root growth, number of root and shoot growth increased with treatment of 100 mg/l or 500 mg/l NAA. Consequently, optimum condition of hardwood cutting was at treatment with 100 mg/l or 500 mg/l NAA. When plantlets from hardwood cuttings were exposed to the field condition, after 7 months survival rate was 73.3% without shading while $91.1{\sim}94.4%$ at shading conditions. In the green house, however, survival rate of plantlets were $95.6{\sim}97.8%$ without shading. The growth of plantlets was different depending on sites and shading conditions. Particularly, the best growth was obtained when the plantlets were grown in shading conditions. It indicates that relative humidity and light intensity are correlated with the growth in the low altitude area.

• Korean Journal of Environment and Ecology
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• v.6 no.1
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• pp.44-54
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• 1992

A survey of subalpine zone (altitude is 1, 440-1, 700m) in Mt. Halla, was conducted using 37 sampling plots of l00$m^2$ size. The classification by TWINSPAN and RA. DCA ordination were applied to the study area in order to classify them based on woody plants and environmental variables. And to investigate vegetation deterioration by livestock's and user's impact on subalpine zone, seventeen plots were set up. By TWINSPAN techniques, the plant community were divided into five groups by the altitude. The dividing groups are Abies koreana-Taxus cuspidata community, Rhododendron yedoense var. poukhanense-Rh. mucronulatum var. ciliatum-Sasa quelpaertensis community, Ilex crenata- Elaeagnus umbellata-S. quelpaertensis community, Rh. yedoense var. poukhallense-Juniperus chinensis var. sargentii-Rh. mucronulatum var. ciliatum community and Empetrum nigrum var. japonicum-J. chinensis var. sargentii community. The species divirsity of plant community were decreased severely by livestock's and user's impact.

• Journal of Environmental Impact Assessment
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• v.23 no.2
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• pp.119-136
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• 2014

This work aims to select the potentially vulnerable plant species against climate change at alpine and subalpine belts of Mts. Sorak, Jiri, and Halla, from central, southern, southern insular high mountains of the Korean Peninsula, respectively. The selection of global warming related vulnerable plants were performed by adapting various criteria, such as flora, endemicity, rarity, floristically specific and valuable species, species composition at mountain summits, horizontal and vertical ranges of individual species, and their distributional pattern in the Korean Peninsula. Line and quadrat field surveys along the major trails from all directions at height above 1,500 meters above sea level of Mts, Sorak, Jiri and Halla were conducted each year during spring, summer, and autumn from 2010 to 2011. Based upon above mentioned eight criteria, high level of climate change related potentially vulnerable arboral plants, such as Rhododendron aureum, Taxus caespitosa, Pinus pumila, Oplopanax elatus, Vaccinium uliginosum, and Thuja koraiensis are noticed from at subalpine belt of Mt. Sorak. Species of Abies koreana, Rhododendron tschonoskii, Oplopanax elatus, Taxus cuspidata, Picea jezoensis, and Juniperus chinensis var. sargentii belong to climate change concerned vulnerable species at subalpine belt of Mt. Jiri. High level of climate change related species vulnerability is found at alpine and subalpine belts of Mt. Halla from Diapensia lapponica var. obovata, Salix blinii, Empetrum nigrum var. japonicum, Vaccinium uliginosum, Juniperus chinensis var. sargentii, Taxus cuspidata, Rhamnus taquetii, Abies koreana, Hugeria japonica, Prunus buergeriana, and Berberis amurensis var. quelpartensis. Countermeasures to save the global warming vulnerable plants in situ are required.

• Korean Journal of Plant Resources
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• v.12 no.3
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• pp.221-233
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• 1999

The vegetation and plant resources of Paeaknokdam, the crater of Mt. Halla, was investigated from 1996 to 1998. The vegetation was classified into Diapensia lapponica var. obovata-Tofieldia fauriel association and Festuca ovina-Carex erythrobasis association. The latter was divided into Rhododendron mucronulatum var. ciliatum-Empetrum nigrum var. japonicum subassociation, which was composed partly of Abies koreana-Betula ermani var. saitoana variant. The plant resources of 162 taxa were composed of 49 families, 122 genera, 127 species, 2 subspecies, 30 varieties and 3 formae, of which 63 taxa were newly found in this area. Among the investigated plants, 27 taxa were endemic to Korea or Cheju islands, 39 taxa were rare or endangered, and 65 taxa were identified as alpine plants. One hundred and twenty-one taxa(75.6%) were identified to be useful: 82 for ornament, 75 for medice, 66 for food, 17 for nectar-producing, 15 for pasture, and 10 for industry.

• Journal of the Korean Geographical Society
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• v.36 no.3
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• pp.247-257
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• 2001

The relationships between the distribution of 132 species, 61 genera evergreen broad-leaved trees and shrubs(EBTS) and climatic factors have discussed. The distributional patterns of EBTS were categorized into seven groups on the basis of the number of distributing sites, distributional attitudes and latitudes. Out of seven group. the cold-tolerant EBTS were common at groups I and II, along tilth Empetrum nigrum var. japonicum. Diapensia lapponics subsp. obovata of group III. However, the warmth-tolerant EBTS were rich at groups III. IV V, and VI The lower distributional limits of cold-tolerant EBTS in the groups I and UU decreased as one moves toward south. The upper distributional limit of warmth-tolerant EBTS in the groups III, IV and V decreased with increasing latitude. However. no clear distributional tendency is noticed in the groups VI and VII. The range of warmth-tolerant EBTS appear to show close relationship with the January mean temperature -4 $\^{C}$ and January mean minimum temperature -9$\^{C}$ than others. On the other hand, that of the cold-tolerant EBTS seem to respond well to the August mean temperature 19$\^{C}$ and August mean maximum temperature 26$\^{C}$ than others.