• 제목/요약/키워드: Embryo Transfer Program

검색결과 78건 처리시간 0.027초

고환조직 정자채취술 (TESE)의 정자 상태에 따른 세포질내 정자주입술의 (ICSI)수정률과 임신율 (Fertilization and Pregnancy Rate of Testicular Sperm Extraction(TESE) with Intracytoplasmic Sperm Injection(ICSI))

  • 전진현;김정욱;박용석;이호준;서주태;이유식;손일표;전종영
    • Clinical and Experimental Reproductive Medicine
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    • 제22권2호
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    • pp.149-153
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    • 1995
  • In IVF-ET program, intracytoplasmic sperm injection(ICSI) has been performed with testicular sperm extraction(TESE) in case of no normal spermatozoon could be retrieved from the epididymis. We wished to see whether the quality of testicular sperm affect the fertilization and pregnancy rate in TESE-ICSI cycles(n=40). These cycles were classified into three groups by the total number of normal motile spermatozoa(TNMS) in the TESE sample: i) good sperm(GS) group(n=12), TNMS > 10,000; ii) moderate sperm(MS) group(n=19), 1,000 < TNMS < 10,000; iii) poor sperm(PS) group(n=9), TNMS < 1,000. Among 423 injected oocytes, 307(72.6%) oocytes were normally fertilized and 43 zygotes were cryopreserved. The fertilization rates of GS group(79.3%) and MS group(75.9%) were significantly(p<0.005) higher than PS group(60.2%). After the embryo transfer(n=40), clinical pregnancy was obtained in 14 cycles(35.0%) and on-going pregnacy in 13 cycles(32.5%). The clinical and on-going pregnancy rates were similar in each group. From these results it can be concluded that testicular spermatozoa are successfully used with ICSI in IVF-ET program in spite of very poor quality of TESE sample.

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분만후 젖소에 있어서 $PGF_2a+PGF_2a+CIDR$ Program 적용에 의한 발정유기시 BCS의 영향 (Effect of $PGF_2a+PGF_2a+CIDR$ Program on Estrous Response in Holstein with Varying BCS in Early Postpartum)

  • 백광수;박성재;박수봉;김현섭;이현준;이왕식;전병순;안병석;김재기;정경용;손준규
    • 한국수정란이식학회지
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    • 제20권3호
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    • pp.279-287
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    • 2005
  • 본 연구는 젖소에 있어서 분만후 난소 활동의 재개 및 분만후 40일째 BCS가 $PGE_2a+PGE_2a+CIDR$ (PPC) program 적용에 미치는 영향을 구명하기 위하여 2002년부터 2003년까지 2년간에 걸쳐 축산연구소 및 천안 소재 전문 경영체 젖소농 장에서 사육중인 홀스타인 착유우를 대상으로 분만 후 난소 활동의 재개를 조사하기 위한 착유우21두 및 분만후 40일째 BCS가 PPC program 적용에 미치는 영향을 구명하기 위한 착유우 40두를 공시하여 수행한 결과 다음과 같은 성적을 얻었다. 1 분만후 처음으로 유즙중의 progesterone농도가 1.0ng/mL 이상을 나타내는 비율은 10${\~}$30일이 $42.9\%$, 31${\~}$50일이 $19.1\%$였고 분만 후 50일까지 1.0ng/mL 이상을 나타내지 않은 경우가 $38.1\%$였다. 2. 분만후 40일째부터 PPC program을 투입한 결과 1차 및 2차 $PGF_2a$ 처리에 대한 발정반응은 각각 $47.5\%$$52.4\%$였고, $PGF_2a+PGF_2a$ (PP) program투입에 의해 40두중 30두($75\%$)가 발정 반응을 나타내었다. PP program 투입에 의해 발정 반응을 나타내지 않은 10두에 대한 CIDR 처리의 발정 반응은 $50\%$를 나타내었다. 분만후 40일째에 PPC program 을 적용한 결과 40두중 35두가 발정 반응 ($87.5\%$)을 나타내었다. 3. 1차 및 2차 $PGF_2a$ 처리에 따른 발정 반응은 BCS 2.50 이하의 경우 13두중 8두($61.5\%$), BCS 2.75${\~}$3.50의 경우 27두중 22두($81.5\%$)로 2.75${\~}$3.50의 경우가 2.50 이하의 경우에 비하여 높은 발정반응을 나타내었다. 4. 2차 $PGF_2a$ 처리후 발정 반응을 나타내지 않은 개체에 대하여 CIDR을 처리한 결과, BCS 2.50이하의 경우 5두중 2두에서 발정 반응($40.0\%$)을 나타내었고 BCS 2.75${\~}$3.50의 경우 5 두중 4두에서 발정 반응($80\%$)을 나타내었다. 5. 분만후 40일째부터 PPC program을 적용하여 발정 반응을 검토한 결과 BCS 2.50 이하의 경우 13두중 10두가 발정 반응($76.9\%$)을 나타내었고 BCS 2.75${\~}$3.5의 경우 27두중 26두가 발정 반응($96.3\%$)을 나타내어 2.75${\~}$3.5의 경우가 2.50 이하의 경우에 비하여 높은 발정반응을 나타내었다.

인간 포배란의 유리화동결 융해 후 임신 및 분만에 관한 연구 (Clinical Study on the Successful Pregnancy and Delivery after Transfer of Human Blastocysts Cryopreserved by Vitrification)

  • 최동희;정형민;정미경;이숙환;남윤성;박찬;곽인평;윤태기
    • Clinical and Experimental Reproductive Medicine
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    • 제27권4호
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    • pp.367-372
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    • 2000
  • Objective: This study was performed to evaluate whether vitrification method could be used for the cryopreservation of human blastocysts derived from IVF program. Methods: Surplus embryos were obtained from consented IVF patients. Controlled ovarian hyperstimulation was done with midluteal GnRH agonist, gonadotropin and hCG. After oocyte retrieval and insemination, fresh embryo transfer was done at $4{\sim}8$ cell stage. The surplus embryos after ET were cultured in blastocyst medium up to 6 days after oocyte retrieval. Obtained blastocysts were cryopreserved with our vitrification method. Blastocysts were exposed to 1.5 Methylene glycol (EG) in phosphate buffered saline (PBS) for 2.5 minutes, followed by 5.5 M EG plus 1 M sucrose for 20 seconds. Then 1 to 3 blastocysts were mounted on electron microscope (EM) grid and the grid was plunged into liquid nitrogen for storage. For thawing, blastocyst-containing EM grids were sequentially transferred in 1.0 M, 0.5 M, 0.25 M, 0.125 M and 0 M sucrose solution at the intervals of2.5 minutes. And blastocysts were cultured for about 6 hours and only re-expanded blastocysts were transferred to uterus of the patients on 4 to 5 days after ovulation in natural cycle or on 18 to 19 day of artificial cycle. Results: From Oct. 1998 to Jul. 1999, 34 patients were agreed to participate in this study. The mean age and duration of infertility of the patients were 31.6 years and 4.1 years, respectively. Among 34 cycles. replacements could be done in 20 cycles (58.8%). A total 93 blastocysts were thawed and 48 (51.6%) of them survived. Thirty-eight blastocysts, mean 1.9 embryos per patient, were transferred, resulting in 5 clinical pregnancies which consisted of 1 triplet, 2 sets of twins and 2 singleton pregnancies. The pregnancy rate per transfer was 25% and implantation rate was 23.6%. Five patients delivered 7 healthy babies including 2 sets of twins at term. Conclusion: Successful pregnancies and deliveries were established after transfer of vitrified human blastocysts. Vitrification using ethylene glycol as cryoprotectant and electron microscope grid is a rapid and simple method that can be effectively applied for the cryopreservation of human blastocysts.

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OPU(Ovum Pick-Up) 채란기간이 난자 및 수정란 생산에 미치는 영향 (Effect of OPU (Ovum Pick-Up) Duration on the Rate of Collected Ova and In Vitro Produced Blastocyst Formation)

  • 진종인;권태현;최병현;김성수;조현태;공일근
    • 한국수정란이식학회지
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    • 제25권1호
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    • pp.15-20
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    • 2010
  • 본 연구에서는 OPU를 통한 체내 유래 난자를 이용한 수정란 생산 시 1개월에서 6개월까지의 연구 기간에 따른 난포 생성수, 난자 회수율, 난자 등급율, 배반포 생성률을 분석하여 공란우의 활용 기간에 관하여 조사하였다. 1. 채란 기간에 따른 난포 생성수는 1개월에서 5개월까지의 난포 생성수는 $7.78{\pm}0.57$, $9{\pm}0.68$, $6.96{\pm}0.60$, $7.18{\pm}0.83$, $6.19{\pm}0.82$개로 유의적인 차이가 없었으나, 6개월째에는$2.83{\pm}0.39$개로 급격하게 줄어든 것을 알 수 있었다. 2. 채란 기간에 따른 난자 회수개수는 1개월에서 3개월까지는 $6.0{\pm}0.5$, $6.2{\pm}0.7$, $5.2{\pm}0.6$개로 유의적인 차이가 없었으나, 4개월부터 6개월까지 $3.7{\pm}0.5$, $2.8{\pm}0.4$, $1.2{\pm}0.2$개로 유의적으로 적어지는 것을 알 수 있었다. 난자 회수율 또한 1개월에서 3개월까지는 76.9%, 69.3%, 75.2%로 유의적인 차이가 없었으나, 4개월에서 6개월까지는 51.1%, 44.6%, 41.2%로 유의적으로 낮아지는 것을 알 수 있었다. 3. 채란 기간에 따른 난자 등급은 Grade 1, 2등급 출현율이 1개월부터 3개월까지 Grade 1은$1.8{\pm}0.3$, $2.2{\pm}0.3$, $1.9{\pm}0.3$, Grade 2는 $2.1{\pm}0.3$, $2.4{\pm}0.3$, $2.1{\pm}0.3$로 유의적으로 차이가 없었으나, 4개월, 5개월에는 Grade 1은 $1.73{\pm}0.27$, $1.14{\pm}0.33$, Grade 2는 $1.33{\pm}0.28$, $0.67{\pm}0.16$로 유의적으로 줄어들었으며, 6개월에는 Grade 1은 $0.3{\pm}0.1$, Grade 2는 $0.5{\pm}0.2$로 현저히 줄어든 것을 알 수 있었다. Grade 3과 4의 출현율은 유의적으로 차이가 없었다. 4. 채란 기간에 따른 배반포 발달율은 1개월에서 3개월까지의 배반포 발달율은 37.2%, 40.4%, 44.6%로 높은 발달율을 보이며 차이가 없었으나, 4개월째에는 24.8%, 5개월째에는 29.3%, 6개월째에는 28.6%로 급격하게 줄어드는 것을 알 수 있었다. 1회 채란 시 수정란의 생산 개수 또한 1개월에서 3개월까지는 $2.2{\pm}0.3$, $2.5{\pm}0.3$, $2.3{\pm}0.4$개로 유의적인 차이가 없었으나, 4개월째부터 6개월까지는 $0.9{\pm}0.2$, $0.8{\pm}0.2$, $0.3{\pm}0.2$개로 현저히 줄어드는 것을 알 수 있었다. 이상의 결과에서 약 3개월까지는 공란우의 활용이 효율적인 점과 4개월부터는 난자 회수와 그에 따른 1회 채란 시 배반포의 생성 수 또한 점차 적으로 줄어드는 것을 알 수 있었다. 따라서, 공란우의 활용을 최대 3개월까지 활용하고, 교체한다면 3~4일 간격으로 1주일에 2회 OPU 방법으로 체내 난자를 채란 후 수정란을 생산하는 시스템은 1두에 3개월간 약 48개의 이식 가능한 수정란을 생산할 수 있고, 산술적으로 년간 1두당 약 200개의 이식 가능한 OPU 유래 수정란을 생산할 수 있을 것이다. 따라서 공란우를 3개월 활용 후 교체를 하는 것이 경제적이며 효율적인 방법이라고 사료된다.

Immunobead 검사로 검출된 항정자 항체가 인간 난자의 체외 수정 및 분할에 미치는 영향 (The Effect of Antisperm Antibodies Detected by Immunobead Binding Assay on Fertilization and Cleavage of Human Oocytes In Vitro)

  • 정동근;신창재;문신용;장윤석
    • Clinical and Experimental Reproductive Medicine
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    • 제16권2호
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    • pp.153-160
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    • 1989
  • The effect of antisperm antibodies (ASA) on the human in vitro fertilization (lVF) process was evaluated by analyzing the IVF data between October and December 1988 at Seoul National University Hospital prospectively. The immunobead test (IBT) was used to identify Ig G, Ig A, and Ig M in the serum, semen, and follicular fluid from 93 couples undergoing in vitro fertilization-embryo transfer (lVF-ET ) . The fertilization rate in couples with ASA to sperm head of at least one isotype in female serum (n= 10) was significantly less than that in couples without ASA to sperm head (n=83; 28.5% versus 45.3% , p=0.028). The presence of ASA to sperm head in follicular fluid (n=8) also reduced fertilization rate from 45.3% to 24.4% (p=O.0l3). However, ASA binding to sperm head in male serum and semen did not predict fertilization. Similarly, ASA binding to sperm tail and tail-tip did not reduced the oocyte fertilization rate significantly in any of the fluids tested. The zygote cleavage rate was not reduced in the presence of ASA. These results suggest that the presence of ASA to sperm head in female serum and follicular fluid is associated with reduced fertilization in IVF-ET. Another observation is that the oocyte that do fertilize in the presence of antisperm antibodies can subsequently proceed with normal cleavage. The results of this investigation therefore suggest that the IBT is a useful test forscreening of women participat.ing IVF-ET program.

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Effects of dynamic oxygen concentrations on the development of mouse pre- and peri-implantation embryos using a double-channel gas supply incubator system

  • Lee, Seung-Chan;Seo, Ho-Chul;Lee, Jaewang;Jun, Jin Hyun;Choi, Kyoo Wan
    • Clinical and Experimental Reproductive Medicine
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    • 제46권4호
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    • pp.189-196
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    • 2019
  • Objective: We aimed to evaluate the effects of different oxygen conditions (20% [high O2], 5% [low O2] and 5% decreased to 2% [dynamic O2]) on mouse pre- and peri-implantation development using a novel double-channel gas supply (DCGS) incubator (CNC Biotech Inc.) to alter the oxygen concentration during in vitro culture. Methods: The high-O2 and low-O2 groups were cultured from the one-cell to the blastocyst stage under 20% and 5% oxygen concentrations, respectively. In the dynamic-O2 group, mouse embryos were cultured from the one-cell to the morula stage under 5% O2 for 3 days, followed by culture under 2% O2 to the blastocyst stage. To evaluate peri-implantation development, the blastocysts from the three groups were individually transferred to a fibronectin-coated dish and cultured to the outgrowth stage in droplets. Results: The blastocyst formation rate was significantly higher in the low-O2 and dynamic-O2 groups than in the high-O2 group. The total cell number was significantly higher in the dynamic-O2 group than in the low-O2 and high-O2 groups. Additionally, the apoptotic index was significantly lower in the low-O2 and dynamic-O2 groups than in the high-O2 group. The trophoblast outgrowth rate and spread area were significantly higher in the low-O2 and dynamic-O2 groups than in the high-O2 group. Conclusion: Our results showed that a dynamic oxygen concentration (decreasing from 5% to 2%) had beneficial effects on mouse pre- and peri-implantation development. Optimized, dynamic changing of oxygen concentrations using the novel DCGS incubator could improve the developmental competence of in vitro cultured embryos in a human in vitro fertilization and embryo transfer program.

체외수정시술을 위한 성선자극호르몬 과배란유도에 Poor Response를 나타낸 환자에서 GnRH Analogue의 사용 (GnRH Analogue in Controlled Ovarian Hyperstimulation for Gonadotropin Poor Responder)

  • 김선행;이희경;구병삼
    • Clinical and Experimental Reproductive Medicine
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    • 제20권1호
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    • pp.37-43
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    • 1993
  • In 27 patients with the past history of poor response to the gonadotropin superovulation induction due to poor follicular growth or permature surge of endogenous luteinizing hormone, the effectiveness of pituitary supperssion with the gonadotropin releasing hormone agonist(GnRH-a) in in vitro fertilization(IVF) program was evaluated in 43 cycles using a combination regimen of D-Trp-6 LHRH(Decapeptyl, Ferring)and FSH/hMG from June, 1989 to August, 1990 at Korea University Hospital IVF Clinic. At midluteal phase of menstrual cycle, Decapeptyl-CR was administered by long-term protocol to minimize initial agonistic effect of endogenous gonadotropins. After the confirmation of pituitary suppression, about 2-3 weeks after GNRH-a administration, ovarian follicle growth was stimulated with FSH/hMG and followed by transvaginal ultrasonic measurement of follicle size and by monitoring of serm E2 and LH if necessary. When compared with the control group stimulated with gonadotropin regimen only, the cancellation rate and occurrence rate of premature LH surge during gonadotropin treatment were significantly lower in study group(11.6% and 2.4%, respectively). There is no significant differences in the mean number of aspirated oocytes, fertilization/cleavage rate, embryo transfer(ET) rate, and mean number of embryos transferred between the two groups. The pregnancy rate per treatment cycle, 16.3%, and per ET cycle, 23.3%, were significantly higher in the study group compared with those of control group. These data suggest that GnRH-a therapy is effective for previous poor responder In gonadotropin superovulation induction for IVF.

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I.V.F.를 위한 난자채취 방법의 비교연구 (A Comparative Study on Oocyte Retrieval for I.V.F.)

  • 김서경;김영태;김선행;나중열;구병삼
    • Clinical and Experimental Reproductive Medicine
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    • 제17권2호
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    • pp.115-121
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    • 1990
  • Ultrasonically guided oocyte collection gradually replaces laparoscope in many IVF center. In present study, we compare the efficacy of both methods in our IVF program. Totally 377 cycles which were undertaken in vitro fertilization treatment were divided into 2 groups. Ultrasonically guided transvaginal follicular aspiration was performed in 188 cycles and laparoscopic follicular aspiration was performed in 189 cycles under local anesthesisa. The mean age for both groups was similar. Follicular recruitment was achieved with human menopausal gonadotropin (hMG) or a com bination of clomiphene citrate and hMG or a combination of FSH and hMG. In the ultrasonically guided aspiration group, 1821 follicles were aspirated with 61.8% of recovery rate (1125 oocytes), 81.5% of embryo transfer rate (145 cycles) and (17%), 26 cases intrauterine pregnancies were estabilished. In the laparoscopic group, 604 follicles were aspirated with 68.7% recovery rate (445 oocytes) and a 79.9% ET rate (127 cycles), 11 cases (8.7%) intrauterine pregnancies were estabilished. A valid comparison of these data is not possible because the 2 groups are dissimilar for factors known to influence oocyte development and recovery. No statistically significant differences could be demonstrated between 2 groups in all but the recovery rate and clinical pregnancy rate, In ultrasound group, the clinical pregnancy rate was significantly higher than that of laparoscope group. The potentially detrimental effect of CO2 pnemoperitonium present during laparoscope but not in ultrasound guided recovery on ova quality may underlie the observed difference in the clinical pregnancy rate between the 2 groups. Ultrasound guided aspiration seems to be as effective as laparoscopy in terms of oocyte retrieval and conception rate. Furthermore, the procedure is simple and inexpensive, it may replace laparoscopy as a method for oocyte collection in most patients who undergo IVF.

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체외수정시술시 유전자 재조합 난포자극호르몬제의 효용성 (The Efficacy of Recombinant Human Follicle Stimulating Hormone (rhFSH) in Human IVF-ET Program)

  • 한국선;이홍복;송인옥;박용석;변혜경;전진현;궁미경
    • Clinical and Experimental Reproductive Medicine
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    • 제29권1호
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    • pp.45-56
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    • 2002
  • Objectives: Recently, recombinant FSH (rFSH) has been manufactured using a Chinese hamster ovary cell line transfected with the gene encoding human FSH. Both rFSH and urinary gonadotropin (uFSH) could be used for controlled ovarian hyperstimulation (COH). However, uFSH implies a number of disadvantages, such as batch-to-batch inconsistency, no absolute source control, dependence on large amounts of urine, low specific activity, and low purity. The purpose of this study was to evaluate the efficacy of rFSH in human IVF-ET program. Materials and Methods: A total of 508 infertile women was enrolled in this study. They are classified into rFSH group (n=177) or uFSH group (n=331), and all of them were matched by age and cause of infertility in same period. The $Puregon^{(R)}$ (Organon, Holland) was used as rFSH, and the Metrodin-$HP^{(R)}$ (Serono, Switzeland) and $Humegon^{(R)}$ (Organon, Holland) was used as uFSH. We subdivided the patients into three age groups. The outcomes of IVF-ET program were analyzed using the statistical package for social sciences (SPSS). Results: There was no significant differences in the level of estradiol on hCG injection day, the numbers of retrieved oocytes, matured oocytes, fertilized oocytes, transferred embryos, frozen embryos between the two groups. The total dose (IU) of gonadotropin for COH was significantly lower in the rFSH group compared to uFSH group ($1339{\pm}5491.1$ vs $2527.8{\pm}1075.2$ IU, p<0.001). Clinical pregnancy rate per embryo transfer in the rFSH group showed increasing tendency, compared to the uFSH group, but there was no statistical significance (35.2% vs 29.3%). Our results demonstrated that the relative efficiency of rFSH compared with uFSH is higher in older patients. Conclusions: The ovarian stimulatory effect and clinical outcome of recombinant FSH was similar to that of the urinary gonadotropin. The IVF-ET cycles with significantly lower dose of gonadotropin in rFSH group showed comparable results. Therefore, we suggest that recombinant FSH is more potent and effective than urinary gonadotropin.

체외수정시술시 예후 인자로서 정자 첨체반응 유발검사의 유용성 (A Stimulated Acrosome Reaction Test as a Prognostic Factor in In Vitro Fertilization)

  • 김정훈;채희동;강은희;추형식;전용필;강병문;장윤석;목정은
    • Clinical and Experimental Reproductive Medicine
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    • 제25권3호
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    • pp.251-260
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    • 1998
  • It is well known that the clinical test for responsibility of accurate fertilization capacity in male partners is very important to diagnose and treat the infertility. However, it has been reported that the traditional semen analysis cannot accurately predict fertilization and pregnancy potential. The present study was performed to evaluate the acrosomal reaction to ionophore challenge (ARIC) test as a prognostic indicator for fertilization of sperm and oocyte in an in vitro fertilization and embryo transfer (IVF-ET) program. From March 1996 to Februry 1997, 30 couples undergoing IVF program were allocated to this study group. All female partners in the study group were 35 years old or less and their serum level of basal follicle stimulating hormone (FSH) and estradiol $(E_2)$ were normal. All the male partners have normal parameters of semen analysis. The ARIC tests were performed on the day of ovum pick up and in vitro insemination in all the male partners. The controlled ovarian hyperstimulation (COH) using luteal long protocol of gonadotropin releasing hormone (GnRH) agonist was used in all couples for IVF-ET. The acrosomal reaction with $10{\mu}l$ of 10% DMSO was induced spontaneously in $10.1{\pm}9.8%$, and acrosomal reaction with calcium ionophore A 23187 was induced in $27.4{\pm}18.1%$, and the ARIC value was $17.4{\pm}16.2%$. There were no significant correlation between the ARIC value and the fertilization rate ($r^2$=0.044, p=0.268). There were also no significant correlation between the ARIC value and the percentage of the grade I, II embryos ($r^2$=0.046, p=0.261). On the basis of above results, it was suggested that ARIC test might not be a useful prognostic indicator for fertilization in IVF-ET in male partners with normal parameters of conventional semen analysis. We guessed that IVF-ET could be performed to the patients primarily without universal appilcation of ARIC test to all male partenrs, and if fertilization failure occurs, the micro assisted fertilization (MAF) such as intracytoplsmic sperm injection (ICSI) might be used as an alternative mode of treatment with acceptable success rate.

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