• Title/Summary/Keyword: Embodiment

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Wolbachia Sequence Typing in Butterflies Using Pyrosequencing

  • Choi, Sungmi;Shin, Su-Kyoung;Jeong, Gilsang;Yi, Hana
    • Journal of Microbiology and Biotechnology
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    • v.25 no.9
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    • pp.1410-1416
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    • 2015
  • Wolbachia is an obligate symbiotic bacteria that is ubiquitous in arthropods, with 25-70% of insect species estimated to be infected. Wolbachia species can interact with their insect hosts in a mutualistic or parasitic manner. Sequence types (ST) of Wolbachia are determined by multilocus sequence typing (MLST) of housekeeping genes. However, there are some limitations to MLST with respect to the generation of clone libraries and the Sanger sequencing method when a host is infected with multiple STs of Wolbachia. To assess the feasibility of massive parallel sequencing, also known as next-generation sequencing, we used pyrosequencing for sequence typing of Wolbachia in butterflies. We collected three species of butterflies (Eurema hecabe, Eurema laeta, and Tongeia fischeri) common to Korea and screened them for Wolbachia STs. We found that T. fischeri was infected with a single ST of Wolbachia, ST41. In contrast, E. hecabe and E. laeta were each infected with two STs of Wolbachia, ST41 and ST40. Our results clearly demonstrate that pyrosequencing-based MLST has a higher sensitivity than cloning and Sanger sequencing methods for the detection of minor alleles. Considering the high prevalence of infection with multiple Wolbachia STs, next-generation sequencing with improved analysis would assist with scaling up approaches to Wolbachia MLST.

Trends and implications of Jjok-Bang research in South Korea based on the perspective of Community-Based Participation and the Social Ecological Model (지역사회기반 참여와 사회생태적 관점에서 본 국내 쪽방지역 주민의 건강증진 역량강화 방안)

  • Kim, Jin Sung;Heo, Hyun-Hee;Chung, Haejoo
    • Korean Journal of Health Education and Promotion
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    • v.32 no.4
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    • pp.79-92
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    • 2015
  • Objectives: The purpose of this study is to identify the trends of jjok-bang research based on the perspective of Community-Based Participatory Research and Social Ecological Model and to provide information for future public health interventions, policy designs, and policy implementations. Methods: Studies used in the systematic review are published from 1999 to 2014 and searched using key words such as 'jjok-bang' and 'single room occupancy' using domestic and international electronic databases. However, there were no studies from abroad published during this period. Search in domestic databases such as KCI, KISS, RISS generated 119 studies. In this paper, 20 cases which meet the criteria of the systematic review were included in the final analysis. Results: The contents of research included are the life of jjok-bang inhabitants(9 cases), the demand for welfare services(4 cases) and welfare need(2 cases), mental health(1 cases), and the history of jjok-bang formation(4 cases). Fourteen cases of empirical study were analyzed focusing Community-Based Participatory Research and Social Ecological Model. Some of research was carried out forming a partnership with various community partners and this trend increased since 2008. There are high frequency of intrapersonal level and interpersonal level studies. However, studies looking at the organization, community or policy level were relatively few. Conclusions: Future studies of jjok-bang area should consider the various social determinants which affect the health delivery system, community organization and policies, as well as individual or community level.

Association between dietary sodium intake and disease burden and mortality in Koreans between 1998 and 2016: The Korea National Health and Nutrition Examination Survey

  • Park, Clara Yongjoo;Jo, Garam;Lee, Juhee;Singh, Gitanjali M.;Lee, Jong-Tae;Shin, Min-Jeong
    • Nutrition Research and Practice
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    • v.14 no.5
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    • pp.501-518
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    • 2020
  • BACKGROUND/OBJECTIVES: Sodium intake is positively associated with blood pressure, which may increase the risk for cardiovascular disease (CVD). Therefore, we assessed the disease burden of CVD attributable to sodium intakes above 2,000 mg/day and prospectively investigated the association between dietary/urinary sodium levels and the risk of all-cause and CVD-mortality using the Korea National Health and Nutrition Examination Survey (KNHNES). SUBJECTS/METHODS: A total of 68,578 and 33,113 participants were included for comparative risk assessment (CRA) analysis and mortality analysis, respectively, and mean follow-up time for mortality was 5.4 years. CRA analysis was used to quantify attributable incidences of stroke, ischemic heart disease (IHD), and deaths attributable to sodium intake between 1998 and 2016. Cox proportional hazard regression model was used to determine the association between sodium intake and all-cause and CVD-mortality. RESULTS: Mean dietary sodium intake decreased over time, reaching 3,647 mg/day in 2016. Similarly, the population attributable fractions of stroke and IHD, and the number of CVD-associated deaths attributable to high sodium intake/excretion also decreased. In terms of association with mortality, when participants were grouped into quartiles (Q) by energy-adjusted sodium intake, those in Q2 had a lower risk of all-cause mortality than those in Q1 with lower intakes. The risk of CVD-associated mortality was higher only in females with high sodium intake in Q4 than those in Q1. CONCLUSIONS: This nationwide data indicates that, in line with previous studies of multiple cohorts, both low and high sodium intakes may be associated with an increased risk of mortality; therefore, the optimal sodium intake for Koreans needs to be revised.

A Study on Embodiment of Animated Character Agent on the Presentation (캐릭터 에이전트 기반 프리젠테이션 모델 구현)

  • 조은경;최영미;주문원
    • Proceedings of the Korea Multimedia Society Conference
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    • 2002.11b
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    • pp.451-456
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    • 2002
  • 본 연구에서는 캐릭터 에이전트 기반 프리젠테이션 모델을 설계하였다. 캐릭터는 인간 대화의 특성을 반영하여 직관적이고 효과적인 커뮤니케이션 방식을 가진다. 그 적용사례로 마이크로소프트 에이전트를 파워포인트에 구현하여 보다 자연스럽고 인간적이며 직관적인 상호작용을 보인다.

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A Study on the Embodiment of Architectural Concept in Felix Nussbaum Museum (펠릭스 누스바움 박물관의 건축 개념 구현 방식에 관한 연구)

  • Chung, Tae-Yong
    • Korean Institute of Interior Design Journal
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    • v.20 no.3
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    • pp.49-58
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    • 2011
  • The purpose of this study is to find out the way of embodying architectural concept in Nussbaum Museum for complementing present abstract and generalized research. This study tries to examine the close relationship between a certain concept and Nussbaum museum through analyzing architectural characteristics and searching its principles by concrete application of concept. The Architectural concept "Museum without exit", which Libeskind applied to Nussbaum Museum, is based on the dramatic life of Jewish painter Felix Nussbaum with relation to Holocaust, physical background of site and features of program, Libeskind Applied his architectural concept from the beginning to the most parts of the museum. His new museum subverts notions and logic of general features of existing museums. Libeskind's architectural concepts are especially applied to site plan, massing, circulation and spatial configuration. "Museum without exit" creates labyrinth like circulation based on stories including Nussbaum's life, his painting tendency and circumstances of those days. It also realize sensuous space through spatial shape and light control. Although some critics criticize that this museum is not suitable for paint collection because of overwhelming strength of architectural space. However, considering this museum's function of a kind of Holocaust museum beyond personal art gallery, Libeskind's 'museum without exit' shows effective realization of architectural concept for Jewish painter Felix Nussbaum's museum.