• KSBB Journal
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• 제8권5호
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• pp.488-494
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• 1993

본 연구에서는 캘리포니아 양귀비(Eschscholtzia californica) 현탁배양세포의 주요 이차대사산물인 benzophenanthridine alkaloid의 생성을 증가시키기 위해 생합성 전구체인 tyrosine, tyramine, dopamine 그리고 L-dopa를 exponential phase 말기에 투여하였다. 그리고 이때 yeast elicitor와 전구체를 동시에 투여한 것과 비교하여 보았다. 그 결과 적당량의 전구체 투여시 benzophenanthridine alkaloid의 생성은 대조구(무처리)에 비해 증가하였다. 그리고 elicitor와 전구체를 동시에 투여해 주었을 때 대조구(elicitor만 처리)에 비해 상당히 증가하였는데, 이것은 elicitor에 의해 유도된 이차대사 관련 효소에 의해 전구체의 biotransformation이 촉진된 것이라 생각된다. 이와 같이 전구체 투여에 의해 이차대사산물의 생성을 증가시키려 할 때 전구체만 투여하는 것보다는 촉진제를 동시에 투여함으로써 최종산물의 생성을 극대화시킬 수 있으리라 생각되며, 식물세포배양의 산업화에 유용하게 이용되리라 생각된다.

• Journal of Microbiology and Biotechnology
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• 제1권3호
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• pp.220-226
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• 1991

Large and rapid increases in benzophenanthridine alkaloid production occured in suspension cultures of Eschscholtzia californica cells treated with elicitors. Response to different biotic elicitors showed that elicitors prepared from yeast extract, Collectotrichum lindemuthianum and Verticillium dahliae induced alkaloid formation. Highest alkaloid accumulation was obtained with $60\;\mu\textrm{g}$ of yeast extract elicitor per gram of fresh cell weight. In time course performance after elicitor addition, more than 40 hours were required to obtain saturated alkaloid accumulation. Compounded silicone fluid, an ideal accumulation phase for two-phase culture of E. californica, accumulated a large amount of alkaloids produced in a specific manner. Elicitation in two-phase culture clearly increased net alkaloid production as well as their concentrations in the accumulation phase.

• Biotechnology and Bioprocess Engineering:BBE
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• 제11권2호
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• pp.105-109
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• 2006

A transformed hairy root clone of Scutellaria baicalensis was established following infection with Agrobacterium rhizogenes ATCC15834. Three root clones of S baicalensis were selected by growth habit and baicalin content. The most active strain-the SR-03 clone-was examined for its growth and baicalin content under various culture conditions. The root growth and baicalin content were maximized in a Schenk and Hildebrandt medium supplemented with 4 and 6% sucrose, respectively. The accumulation of baicalin in transformed hairy roots was enhanced through exposure to various elicitors. Elicitation was attained by the addition of methyl jasmonate, salicylic acid, and various concentrations of fungal cell wall elicitors to the medium. The accumulation of baicalin in the elicited cultures ranged from 10.5 to 18.3 mg/g dry weight of the roots, which was 1.5- to 3-fold the amount attained in controls.

• 한국IT서비스학회지
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• 제13권3호
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• pp.257-274
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• 2014

Social Network Services (SNSs) have grown to be new and promising tools of marketing. By referring to researches done on e-mail viral marketing, this paper operationizes SNS viral marketing effectiveness to accurately reflect marketing success in SNS environment, and tries to identify its affecting factors. As potential affecting factors, fan size, advertisement type, existence of engagement elicitation and incentive are identified. By sampling real advertisement postings on Facebook, we showed that fan size, advertisement type, and engagement elicitation are factors affecting SNS viral marketing success. This research expanded the conventional model of viral marketing into SNS settings to improve understanding on SNS viral marketing. Motivation is discussed as an important factor, and this research showed that viral campaign can be more successful when it triggers internal motivation to engage, but not the external motivation. This research could also be a guide for practitioners on how to post a successful advertisement in SNSs.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제5권10호
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• pp.1733-1750
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• 2011

In the early stages of a software project, it is critical to understand the needs of the customers and elicit their customer requirements. Various requirements elicitation methods have been proposed. However, existing methods still have the limitations such as a limited number of target customers, limited expression of customers' opinions, and difficulty in collecting the customers' opinions continuously. A novel method for eliciting customer requirements is proposed by utilizing a social network service (SNS), which is a shared source of raw information of the customers' needs and opinions. The proposed method is validated to show its effectiveness in overcoming the limitations of existing methods.

• Nuclear Engineering and Technology
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• 제29권1호
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• pp.25-34
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• 1997

This study presents a new approach for expert opinion elicitation process to assess an uncertainty inherent in accident management. The need to work with rare event and limited data in accident management leads analysis to use expert opinions extensively. Unlike the conventional approach using point-valued probabilities, the study proposes the concept of fuzzy probability to represent expert opinion. The use of fuzzy probability has an advantage over the conventional approach when an expert's judgment is used under limited dat3 and imprecise knowledge. The study demonstrates a method of combining and propagating fuzzy probabilities. finally, the proposed methodology is applied to the evaluation of the probability of a bottom head failure for the flooded case in the Peach Bottom BWR nuclear power plant.

• 대한한방내과학회지
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• 제27권3호
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• pp.579-588
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• 2006

Objective : This study was performed to investigate the effect of Astragali Radix on leukocytopenia anaphysis with carcinostatic substance elicitation. Methods : We observe if there were differences in a general tendency by EAR (AR extracts) capacity in the Astragali Radix treated group and the cyclophosphamide treated group, a general tendency of recovery from leukocytopenia over lime and checked for liver and kidney toxicity. Results : In cyclophosphamide treated with EAR 1000mg/kg group, the recovery rate of leukocytopenia increased and there were no differences in liver and kidney functions. Conclusions : Astragali Radix was effective and safe in increasing rate of leukocytopenia anaphysis with carcinostatic substance elicitation and indicated possibility for clinically adaptation.

• 한국디자인학회:학술대회논문집
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• 한국디자인학회 2001년도 Bulletin of The 5th Asian Design Conference International Symposium on Design Science
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• pp.41.1-41
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• 2001

• 한국식물생명공학회:학술대회논문집
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• 한국식물생명공학회 2004년도 생명공학 실용화를 위한 비젼
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• pp.195-195
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• 2004

• 대한약학회:학술대회논문집
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• 대한약학회 2000년도 춘계총회 및 학술대회
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• pp.181.2-181.2
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• 2000