Browse > Article

Baicalin Production in Transformed Hairy Root Clones of Scutellaria baicalensis  

Hwang, Sung-Jin (Department of Oriental Medicine Materials, Dongshin University)
Publication Information
Biotechnology and Bioprocess Engineering:BBE / v.11, no.2, 2006 , pp. 105-109 More about this Journal
Abstract
A transformed hairy root clone of Scutellaria baicalensis was established following infection with Agrobacterium rhizogenes ATCC15834. Three root clones of S baicalensis were selected by growth habit and baicalin content. The most active strain-the SR-03 clone-was examined for its growth and baicalin content under various culture conditions. The root growth and baicalin content were maximized in a Schenk and Hildebrandt medium supplemented with 4 and 6% sucrose, respectively. The accumulation of baicalin in transformed hairy roots was enhanced through exposure to various elicitors. Elicitation was attained by the addition of methyl jasmonate, salicylic acid, and various concentrations of fungal cell wall elicitors to the medium. The accumulation of baicalin in the elicited cultures ranged from 10.5 to 18.3 mg/g dry weight of the roots, which was 1.5- to 3-fold the amount attained in controls.
Keywords
baicalin; elicitation; transformed hairy roots; Scutellaria baicalensis;
Citations & Related Records

Times Cited By Web Of Science : 6  (Related Records In Web of Science)
Times Cited By SCOPUS : 5
연도 인용수 순위
1 Kubo, M., H. Matsuda, M. Tanaka, Y. Kimura, H. Okuda, M. Higashino, T. Tani, K. Namba, and S. Arichi (1984) Studies on Scutallariae radix. VII. Anti-arthritic and antiinflammatory actions of methanolic extract and flavonoid components from Scutellariae radix. Chem. Pharm. Bull. 32: 2724-2729   DOI
2 Hwang, S. J., K. S. Kim, B. S. Pyo, and B. Hwang (1999) Saponin production by hairy root cultures of Panax ginseng CA Meyer. Biotechnol. Bioprocess Eng. 4: 309-312   DOI
3 Wang, Y. D., Y. J. Yuan, and J. C. Wu (2004) Induction studies of methyl jasmonate and salicylic acid on taxane production in suspension cultures of Taxus chinensis var. mairei. Biochem. Eng. J. 19: 259-265   DOI   ScienceOn
4 Mehmetoglu, U. and W. R. Curtis (1997) Effects of abiotic inducers on sesquiterpene synthesis in hairy root and cell suspension cultures of Hyoscyamus meticus. Appl. Biochem. Biotechnol. 67: 71-77   DOI   ScienceOn
5 Gergorio, G. H. and M. L. V. Victor (1997) Effect of acetylsalicylic acid on secondary metabolism of Catharanthus roseus tumor suspension cultures. Plant Cell Rep. 16: 287- 290
6 Yuan, Y. J., C. Li, Z. D. Hu, J. C. Wu, and A. P. Zeng (2002) Fungal elicitor-induced cell apoptosis in suspension cultures of Taxus chinensis var. mairei for taxol production. Process Biochem. 38: 193-198   DOI   ScienceOn
7 Xu, J. F., C. B. Liu, A. M. Han, P. S. Feng, and Z. G. Su (1998) Strategies for the improvement of salidroside production in cell suspension cultures of Rhodiola sachalinensis. Plant Cell Rep. 17: 288-293   DOI
8 Komaraiah, P., R. N. Amrutha, P. B. Kavi-Kishor, and S. V. Ramakrishna (2002) Elicitor enhanced production of plumbagin in suspension cultures of Plumbago rosea L. Enzyme Microb. Technol. 31: 634-639   DOI   ScienceOn
9 Zhang, Y. H., J. J. Zhong, and J. T. Yu (1996) Enhancement of ginseng saponin production in suspension cultures of Panax notoginseng: manipulation of medium sucrose. J. Biotechnol. 51: 49-56   DOI   ScienceOn
10 Ikenaga, T., T. Oyama, and T. Muranaka (1995) Growth and steroidal saponin production in hairy root cultures of Solanum aculeatissimum. Plant Cell Rep. 14: 413-417
11 Park, Y. G., M. H. Kim, J. K. Yang, Y. G. Chung, and M. C. Choi (2003) Light-susceptibility of campothecin production from in vitro cultures of Campotheca acuminate Dec. Biotechnol. Bioprocess Eng. 8: 32-36   DOI   ScienceOn
12 Zhu, U. P. (1998) Materia Medica. pp. 127-135. Harwood Academic Publishers, Amstredam, The Netherlands
13 Knobloch, K. H. and J. Berlin (1980) Influence of medium composition on the formation of secondary compounds in cell suspension cultures of Catharanthus roseus (L). G. Don. Z. Naturforsch. 35C: 551-556
14 Aoki, T., H. Matsumoto, Y. Asako, Y. Matsunaga, and K. Shimomura (1997) Variation of alkaloid productivity among several clones of hairy root and regenerated plants of Atropa belladonna transformed with Agrobacterium rhizogenes 15834. Plant Cell Rep. 16: 282-286
15 Murashige, T. and F. Skoog (1962) A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiol. Plant. 15: 473-479   DOI
16 Misawa, M. (1985) Production of useful plant metabolites. pp. 59-88. In: A. Fiechter (eds.). Advances in Biochemical Engineering Biotechnology. Springer-Verlag, Berlin, Germany
17 Payne, J., J. D. Hamill, R. J. Robins, and J. C. Rhodes (1987) Production of hyoscyamine by hairy root cultures of Datura stramonium. Planta Med. 53: 474-478   DOI   ScienceOn
18 Bulgakov, V. P., G. K. Tchernoded, N. P. Mischenko, M. V. Khodakovskaya, V. P. Glazunov, S. V. Radchenko, E. V. Zvereva, S. A. Fedoreyev, and Y. N. Zhuravlev (2002) Effect of salicylic acid, methyl jasmonate, ethephon and cantharidin on anthraquinone production by Rubia cordifolia callus cultures transformed with the rolB and rolC genes. J. Biotechnol. 97: 213-221   DOI   ScienceOn
19 Tang, W. and G. Eisenbrand (1992) Chinese Drugs of Plant Origin: Chemistry, Pharmacology, and Use in Traditional and Modern Medicine. pp. 919-929. Springer- Verlag, Heidelberg, Germany
20 Schenk, U. K. and A. C. Hildebrandt (1972) Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Can. J. Bot. 50: 199-204   DOI
21 Merkli, A., P. Christen, and I. Kapetanidis (1997) Production of diosgenin by hairy root cultures of Trigonella foenum- graecum L. Plant Cell Rep. 16: 632-636   DOI
22 McKinley, T. C., P. J. Michaels, and H. E. Flores (1993) Is lipooxygenase involved in polyacetylene biosynthesis in Asteraceae? Plant Physiol. Biochem. 31: 835-843
23 Palazon, J., R. M. Cusido, C. Roig, and T. Pinol (1997)Effect of rol genes from Agrobacterium rhizogenes TLDNA on nicotine production in tobacco root cultures. Plant Physiol. Biochem. 35: 155-162
24 Kuzovkina, I. N., A. V. Guseva, I. E. Alterman, and R. A. Karnachuk (2001) Flavonoid production in transformed Scutellria baicalenesis roots and ways of its regulation. Russ. J. Plant Physiol. 48: 448-452   DOI
25 Manthe, B., M. Schulz, and H. Schnabl (1992) Effects of salicylic acid on growth and stomatal movements of Vicia faba L.; evidence for salicylic acid metabolization. J. Chem Ecol. 18: 1525-1539   DOI   ScienceOn
26 Park, Y. G., S. J. Kim, Y. M. Kang, H. Y. Chung, D. T. Prasad, S. W. Kim, Y. G. Chung, and M. S. Choi (2004) Production of ginkolides and bilobalide from optimized the Ginkgo biloba cell culture. Biotehnol. Bioprocess Eng. 9: 41-46   DOI   ScienceOn
27 Ketchum, R. E., C. D. Rithner, D. Qiu, Y. S. Kim, R. M. Williams, and R. B. Croteau (2003) Taxus metabolomics: methyl jasmonate preferentially induces production of taxoids oxygenated at C-13 in Taxus x media cell cultures. Phytochemistry 62: 901-909   DOI   ScienceOn
28 Zhuo, Y., M. Hirotani, T. Yoshikawa, and T. Furuya (1997) Flavonoids and phenylethanoids from hairy root cultures of Scutellaria baicalenesis. Phytochemistry 44: 83- 87   DOI   ScienceOn
29 Lloyd, G. and B. McCown (1980) Commercially feasible micropropagation of Mountain laurel, Kalmia latifolia, by use of shoot tip culture. Comb. Proc. Int. Plant Propagators Soc. 30: 421-427
30 Sakamoto, K., K. Iida, K. Sawamura, K. Hajiro, Y. Asada, T. Yoshikawa, and T. Furuya (1993) Effects of nutrients on anthocyanin production in cultured cells of Aralia cordata. Phytochemistry 33: 357-360   DOI   ScienceOn
31 Parr, A. J., J. Payne, J. Eagles, B. T. Chapman, R. J. Robins, and J. C. Rhodes (1990) Variation in tropane alkaloid accumulation within the Solanaceae and strategies for its exploitation. Phytochemistry 29: 2545-2550   DOI   ScienceOn
32 Zhao, J., W. H. Zhu, and Q. Hu (2001) Selection of fungal elicitors to increase indole alkaloid accumulation in Catharanthus roseus suspension cell cultures. Enzyme Microb. Technol. 28: 666-672   DOI   ScienceOn
33 Giri, A. and M. L. Narasu (2000) Transgenic hairy roots: recent trends and applications. Biotechnol. Adv. 18: 1-22   DOI   ScienceOn
34 Kim, C. H., M. W. Kim, G. J. Choi, H. J. Son, S. R. Ko, S. C. Kim, J. J. Wi, J. J. and J. N. Hu (1991) Analytical Methods of Ginseng Components. pp. 203-208. Korean Ginseng and Tobacco Res. Inst., Korea