• 제목/요약/키워드: Electrophoretic

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Extraction and Electrophoretic Characterization of Rice Proteins

  • Kim, Mee-sook;Jeong, Yoon-hwa
    • Preventive Nutrition and Food Science
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    • 제7권4호
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    • pp.437-441
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    • 2002
  • Rice proteins were extracted from brown and milled rice of five varieties: Kwanganbyeo, Daeanbyeo, Daejinbyeo, Surabyeo, Hwaseongbyeo; and their electrophoretic patterns were analyzed by SDS-PAGE. Albumin was extracted with water, globulin with 5% NaCl, prolamin with 70% ethanol, and glutelin with 0.2 M sodium borate buffer (pH 10.0) containing 0.5% SDS, 0.6% $\beta$-mercaptoethanol. The ratios of albumin : globulin : prolamin : glutelin in the brown rice were 10.8~14.1 : 12.4~16.4 : 3.6~5.3 : 68.6~72.8, and in milled rice were 4.4~5.6 : 10.6~12.0 : 3.9~5.4 : 75.7~79.8. In albumin seven major bands were observed with molecular weights ranging from 14.g~96.8 kDa, in globulin four bands with molecular weights in the range of 14.4~56.9 kDa, prolamin had only one band with a molecular weight of 14.4 kDa, and glutelin had four bands with molecular weights of 14.4 ~ 57.4 kDa. There were no differences in electrophoretic patterns between rice varieties or between brown and milled rice.

Effect of Heat Treatment on the Morphology and Transparency of Thick Inorganic-Organic Hybrid Films Prepared by the Electrophoretic Sol-Gel Deposition of Polyphenylsilsesquioxane Particles

  • Hasegawa, Koichi;Katagiri, Kiyofumi;Matsuda, Astunori;Tatsumisago, Masahiro;Minami, Tsutomu
    • The Korean Journal of Ceramics
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    • 제6권1호
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    • pp.15-20
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    • 2000
  • Thick inorganic-organic hybrid films were prepared on ITO-coated glass substrates by the electrophoretic sol-gel deposition of polyphenylsilsesquioxane particles. The morphology of the deposited films changed from the aggregate of the spherical particles to monolith by heat treatment at temperatures higher than $200^{\circ}C$. Transparency of the films was significantly improved accompanied by the morphological change of the particles. The degree of the morphological change was governed by two factors; maximum heat treatment temperature and heating rate. Transparent thick films of ca. 3$\mu\textrm{m}$ in thickness were obtained only by heat treatment at $400^{\circ}C$ for 2h with rapid heating from room temperature to $400^{\circ}C$. These films obtained were strongly adhered to the ITO-coated glass substrates and has a very smooth surface.

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Curcumin-Loaded PLGA Nanoparticles Coating onto Metal Stent by Electrophoretic Deposition Techniques

  • Nam, So-Hee;Nam, Hye-Yeong;Joo, Jae-Ryang;Baek, In-Su;Park, Jong-Sang
    • Bulletin of the Korean Chemical Society
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    • 제28권3호
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    • pp.397-402
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    • 2007
  • Restenosis after percutaneous coronary intervention (PCI) continues to be a serious problem in clinical cardiology. To solve this problem, drug eluting stents (DES) with antiproliferative agents have been developed. Variable local drug delivery systems in the context of stenting require the development of stent manufacture, drug pharmacology and coating technology. We have worked on a system that integrates electrophoretic deposition (EPD) technology with the polymeric nanoparticles in DES for local drug delivery and a controlled release system. The surface morphology and drug loading amount of DES by EPD have been investigated under different operational conditions, such as operation time, voltage and the composition of media. We prepared poly-D,L-lactide-co-glycolic acid (PLGA) nanoparticles embedded with curcumin, which was done by a modified spontaneous emulsification method and used polyacrylic acid (PAA) as a surfactant because its carboxylic group contribute negative charge to the surface of CPNPs (?53.5 ± 5.8 mV). In the process of ‘trial and error' endeavors, we found that it is easy to control the drug loading amount deposited onto the stent while keeping uniform surface morphology. Accordingly, stent coating by EPD has a wide application to the modification of DES using various kinds of nanoparticles and drugs.

컬러 모노 카메라를 이용한 전착 로봇의 자동 제어 (Automatic Control of an Electrophoretic Deposition Robot using a Color Mono Camera)

  • 박재병
    • 전자공학회논문지SC
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    • 제46권3호
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    • pp.1-7
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    • 2009
  • 본 논문에서는 컬러 모노 카메라를 이용한 자동 전착 로봇 시스템 (Automatic Electrophoretic Deposition Robot System)을 제안한다. 전착 로봇 시스템은 실제 전착 작업 수행을 위한 2자유도 직교 로봇과 로봇 자동 제어를 위한 컬러 모노 카메라로 구성되어 있다. 직교 로봇은 전착 작업 특성상 10mm/s까지 저속 구동이 가능하도록 스크류 (Screw)를 사용하여 감속 구동하였다. 컬러 모노 카메라는 로봇과 비커에 부착된 컬러 마커를 인식하여 각각의 위치를 측정하고 측정된 위치를 기반으로 로봇을 제어한다. 또한, 카메라에 의해 비커에 부착된 컬러 마커의 조합을 인식하여 다양한 작업 변수를 갖는 전착 작업을 판단한다. 제안된 자동 전착 로봇 시스템의 효율성을 입증하기 위해 전착 작업 실험을 수행하였고 그 결과를 제시하였다.

진동정렬 EPD YBCO 후막테이프의 초전도 특성 개선 (Superconducting Properties of Shaky-aligned EPD Thick Film of YBCO Tape)

  • 소대화;조용준;박성범;전용우
    • 한국전기전자재료학회:학술대회논문집
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    • 한국전기전자재료학회 2003년도 하계학술대회 논문집 Vol.4 No.1
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    • pp.111-114
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    • 2003
  • In order to improve the surface uniformity and the conduction properties of the fabricated YBCO thick films, a system that applies alternating field vertically to the EPD field has been developed for the first time and applied to the electrophoretic deposition process. The applied alternating electric field caused a force to be exerted on each YBCO particle and resulted in a shaking of the particle in the direction of applied electric field, accomplishing a uniform particle orientation. The usual commercial electrical power was used for the vertically applied alternating voltage and the induced electric field was 25-120 V/cm at 60Hz. The thick film fabricated by the method developed in this paper showed better surface uniformity without crack and porosity and improved film characteristics such as critical temperature ($T_{c,zero}$ : 90 K) and critical current density ($2354\;A/cm^2$). Therefore, it is expected that the shaky-aligned electrophoretic deposition method can be used to fabricate superconductor films through a simpler process and at less expense.

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Flexible Microelectronics; High-Resolution Active-Matrix Electrophoretic Displays

  • Miyazaki, Atsushi;Kawai, Hideyuki;Miyasaka, Mitsutoshi;Nebashi, Satoshi;Shimoda, Tatsuya;McCreary, Michael
    • 한국정보디스플레이학회:학술대회논문집
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    • 한국정보디스플레이학회 2005년도 International Meeting on Information Displayvol.I
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    • pp.575-579
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    • 2005
  • A beautiful, flexible active-matrix electrophoretic display (AM-EPD) device is reported. The flexible AM-EPD device has a $40.0{\times}30.0\;mm^2$ display area, measures about 0.27 mm in thickness, weighs about 0.45 g and possesses only 20 external connections. The flexible AM-EPD device displays clear black-and-white images with 5 gray-scales on $160{\times}120$ pixels. The display is free from residual image problems, because we use an area-gray-scale method on $320{\times}240$ EPD elements, each of which is driven with binary signals. Each pixel consists of 4 EPD elements. In addition, since the response time of the electrophoretic material is as long as approximately 400 ms and since the display possesses a large number of EPD elements, we have developed a special driving method suitable for changing EPD images comfortably. A complete image is formed on the AM-EPD device, consisting of a reset frame and several, typically 6, image frames.

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측면진동보조전계 전기영동 전착방식을 적용한 YBCO 초전도 후막의 제작 (Fabrication of YBCO Superconducting Thick Film by Use of Lateral Shaky Field Assisted EPD Method)

  • 소대화;전용우
    • 한국전기전자재료학회논문지
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    • 제16권11호
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    • pp.1041-1046
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    • 2003
  • In order to improve the surface uniformity and the conduction properties of the fabricated YBCO thick films, a system that applies alternating field vertically to the EPD field has been developed for the first time and applied to the electrophoretic deposition process. The applied alternating electric field, so called Shaky Alternating Assisted Field, caused a force to be exerted on each YBCO particle and resulted in a shaking of the particle in the direction of applied electric field, accomplishing a uniform particle orientation. The usual commercial electrical power was used for the vertically applied alternating voltage and the induced electric field was 25-120 V/cm at 60Hz. The thick film fabricated by the method developed in this paper showed better surface uniformity without crack and porosity and improved film characteristics such as critical temperature (Tc,zero = 90 K) and critical current density (2354 A/$\textrm{cm}^2$), Therefore, it is expected that the shaky-aligned electrophoretic deposition method can be used to fabricate superconductor films through a simpler process and at less expense.

담수산 이매패류 패각내 유기각질 단백질 특성 (Characterization of the Organic Matrix Protein in the Freshwater Pelecypod Shells)

  • 박성빈;조동현
    • 한국패류학회지
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    • 제3권1호
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    • pp.3-14
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    • 1987
  • The electrophoretic and innunological cnalyses of organic matrices in the shells of freshwater bivalves were made in order to dlucidate the biochemical characteristics and species-specific differinces of the applied shells, The water-soluble and insoluble matrices of four species of freshwater bivalves, Andodonta fukudai, Unio douglasiae, Lanceolaria and Chrbicula fluminea, were used as analytical materials, There was non-identity in immuno affinity between anti soluble matrix(anti-Sm) and anti insoluble matrix(anti-ISM)sera against the organic matrix of Andodnta fukudai. The SMs of four species (S. fukudai, U.douglasiae, L. acrorhyncha, and C. fluminea) showed the differences in the precipitate arcs at the level of family, though ISMs did mot show differences. In the electrophoretic analysis, all foru species had two SDS-electrophoretic bands of SM, of which molecular wights appeared to be lower than 55,000, shereas the native organic matrices of foru speceis had higher molecular weighrs than those from SDS-dldctrophoresis. Only calcium ion among many ions in extrapallial fluid(EPF) caused SM to change into insoluble molecules, thus the EPF pretreated with Ca++did not form the precipitate arc when did the immuno diffusion whth anti SM serum. ISM precursor may be polymerized into macromolecules like periostracin, a precusor of periostracum, judging from the similat polymerization patterns in 0.1M Tris formate buffer(pH 3); they may be made insoluble macromolecules due to their strong natrue of hydrophobicity.

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Binder-free Sn/Graphene Nanocomposites Prepared by Electrophoretic Deposition for Anode Materials in Lithium Ion Batteries

  • Bae, Eun Gyoung;Hwang, Yun-Hwa;Pyo, Myoungho
    • Bulletin of the Korean Chemical Society
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    • 제34권4호
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    • pp.1199-1204
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    • 2013
  • Nanocomposites consisting of Sn nanoparticles and graphene oxide (GO) were electrophoretically deposited onto Cu current collectors that was used for anodes in Li ion batteries (LIBs). In order to optimize the electrochemical performance of nanocomposites as an anode material by controlling the oxygen functionality, the GO was subjected to $O_3$ treatment prior to electrophoretic deposition (EPD). During thermal reduction of the GO in the nanocomposites, the Sn nanoparticles were reduced in size, along with the formation of SnO and/or $SnO_2$ at a small fraction, relying on the oxygen functionalities of the GO. The variation in the duration of time for the $O_3$ irradiation resulted in a small change in total oxygen content, but in a significantly different fraction of each functional group in the GO, which influenced the Sn nanoparticle size and the amount of SnO (and/or $SnO_2$). As a result, the EPD films prepared with the GO that possessed the least amount of carboxylic groups (made by treating GO in an $O_3$ environment for 3 h) showed the best performance, when compared with the nanocomposites composed of untreated GO or GO that was $O_3$-treated for a duration of less than 3 h.

Fabrication of SiCf/SiC Composites using an Electrophoretic Deposition

  • Lee, Jong-Hyun;Gil, Gun-Young;Yoon, Dang-Hyok
    • 한국세라믹학회지
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    • 제46권5호
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    • pp.447-451
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    • 2009
  • Continuous SiC fiber-reinforced SiC composites ($SiC_f$/SiC) were fabricated by electrophoretic deposition (EPD). Nine types of slurries with different powder contents, binder resin amounts and slurry pH were deposited on Tyranno$^{TM}$-SA fabrics by EPD at 135 V for ten minutes to determine the optimal conditions. Further EPD using the optimum slurry conditions was performed on fabrics with four different pyrolitic carbon (PyC) thicknesses. The density of the hot-pressed composites decreased with increasing PyC thickness due to the difficulty of infiltrating the slurry into the narrow gaps between the fibers. On the other hand, the mechanical strength increased with increasing PyC thickness despite the decrease in density, which was explained by the enhanced crack deflection with increasing PyC thickness. The $SiC_f$/SiC composites showed the highest density and flexural strength of 94% and 342 MPa, respectively, showing EPD as a feasible method for dense $SiC_f$/SiC fabrication.