• Korean Journal of Bronchoesophagology
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• v.6 no.1
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• pp.44-61
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• 2000

Objectives : This study was designed to compare the morphological changes in the nasal, tracheal and main bronchial mucosa in rats exposed to 0, 0.3, 0.6, 0.9 and 1.2 ppm ozone for 7 days, 6 hours per day. Materials and Methods : We observed the nasal, tracheal and main bronchial mucosa in rats exposed to 0, 0.3, 0.6, 0.9 and 1.2 ppm ozone for 7days, 6hours per day with LM, SEM and TEM. Results : In light microscopy, influx of inflammatory cells, epithelial hyperplasia, loss of cilia and increased goblet cells were observed in all rats except those exposed to 0.3 ppm. these findings increased with the increase of ozone concentration, but there were no significant differences among the nasal, tracheal and main bronchial mucosa in rats exposed to the same ozone concentration. In scanning electron microscopy, a loss of cilia was observed in rats exposed to 0.3 ppm in some sections and 0.6 ppm and 1.2 ppm in all sections. In transmission electron microscopy, vacuolization of epithelial cells was observed in rats exposed to 0.3 ppm in some sections and 0.6 ppm in all sections. These results suggest that electron microscopic observation is necessary to study morphology of airway mucosa in rats exposed to ozone below 0.3 ppm. And also the morphological changes in nasal septal epithelium may reflect those of tracheal and bronchial epithelium after high concentration ozone-exposure.

• Journal of the Korean Society of Fashion and Beauty
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• v.2 no.2 s.2
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• pp.101-106
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• 2004

Allergic contact dermatitis is a common skin disease resulting from specific immunologic sensitization to topically applied various allergen. The Purpose of this study was to investigate skin morphologic cahnges by light microscopic and scanning electron Microscope, changes of protein band by SDS-PAGE(SDS-polyacrylamide gel electrophoresis) in the skin effectiveness for beruty improvement of Geranium essential oil on skin troubles by surfactant. The results of the study are as follows, 1. From the observed result of FE-Scanning Electron Microscope, groups treated by Geranium essential oil in group treated Geranium essential oil during 1 week in surfactant treated group during 1 week, the group was repaired in irregularity surface of tissue by alleviate-keratinization of Geranium essential oil. 2. As a result of protein analysis the group treated on surfactant was rised protein upper range of 97,0004a11on by hyper-keratinization and group treated during 1 week by surfactant was decreased protein below range of 43,000dalton.

• Applied Microscopy
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• v.28 no.4
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• pp.577-590
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• 1998

Puromycin aminonucleoside (PAN) nephropathy was induced in a group of Sprague-Dawley rat by a single dose of intraperitoneal Injection to study an ultrastructural change of glomerulus. The experimental rats developed proteinuria three days after PAN injection. Electron microscopic studies of glomeruli showed the loss of epithelial foot processes, formation of cytoplasmic vacuoles, microvillous formation and increased numbers of lysosomes in the cytoplasm of podocytes. It is strongly suggested that proteinuria in PAN nephrosis may be primarily due to a glomerular epithelial lesion, leading to focal disarray of anionic sites or focal defects in the epithelial covering of the basement membrane. The loss of anionic sites in the basement membrane nay be caused by the foot process fusion and the epithelial detachment from the basement membrane.

• Journal of Korean Neurosurgical Society
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• v.37 no.3
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• pp.217-222
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• 2005

Objective: To achieve optimal fit of implant, it is necessary to bend the implant during spine surgery. Bending procedure may decrease stiffness of plate especially made of titanium and stainless steel. Typically titanium suffers adverse effects including early crack propagation when it is bent. We investigate whether 6 degree bending of titanium plates would decrease the stiffness after full cyclic loading by comparing with non-bending titanium plates group. Methods: Authors experimented 40 titanium alloy plates of 57mm in length, manufactured by 5 different companies. Total 40 plates were divided into two groups (20 bent plates for experimental group and 20 non-bent plates for control group). Twenty plates of experimental group were bent to 6 degree with 3-point bending technique and verified with image analyzer. Using the electron microscope, we sought for a initial crack before and after 3-point bending. Mechanical testing by means of 6000 cyclic axial-compression loading of 35N in compression with moment arm of 35mm-1.1 Nm was conducted on each plate and followed by the electron microscopic examination to detect crack or fissure on plates. Results: The stiffness was decreased after 6000 cyclic loading, but there was no statistically significant difference in stiffness between experimental and control group. There was no evidence of change in grain structure on the electron microscopic magnification. Conclusion: The titanium cervical plates can be bent to 6 degree without any crack or weakness of plate. We also assume that minimal bending may increase the resistance to fatigue fracture in cervical flexion-extension movement.

• Journal of Nutrition and Health
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• v.15 no.4
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• pp.277-289
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• 1982

Because of a high degree of unsaturation of perilla seed oil vitamin E deficiency can be produced in chicks by using this particular oil diet. It is therefore convenient to use this oil for elucidating more detailed function (s) of vitamin E. The present study was undertaken to investigate the nutritional and pathological aspects both of high PUFA and of low vitamin E in the diet eventually to elucidate the function(s) of vitamin E. The present study examines the relationships between PUFA, vitamin E and Se in the experimental diets by measuring vitamin E levels both in serum and in liver and activities of SGPT and SGOT and by examining electron micrographs of the chick hepatocytes. Vitamin E concentrations in serum and liver responded to dietary treatments. Serum levels of vitamin E were more affected than those in liver, the values of groups IV(15% fat,- Vit.E) and V(15% fat,-Vit E,-Se) were significantly lower than those of groups I (5% fat) and 111 (15% fat) with P/S ratio of 1. The activities of SGOT and SGPT were found not to be different significantly among different diet groups. Electron microscopic observations of the chick hepatocyte revealed degeneration of mitochondria and appearance of vesicles in the cytoplasm of groups fed diets high in PUFA and deficient in vitamin E alone or deficient in both vitamin E and Se. In the same group pyknotic nuclei and deterioration of the sinusoidal border, showing diminution in microvilli were also observed. More detailed studies concerning biochemical aspects should be carried out by using radioisotopes both in the in vitro and in vivo systems and morphological study should have a focus on the changes in nucleus which seems already suggestive in the present observation.

• The Journal of the Korean dental association
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• v.22 no.5 s.180
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• pp.439-446
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• 1984

The purpose of this study was observe the effects of excessive occlusal force on gingival fibroblasts and collagen fibers of dog. The experiment was performed in 5 dogs. 1 dog was used as control and 4 dogs as experimental group. In experimental group, high Sun Platinum casting metal crowns with 2.0mm thick were placed artificially onto the upper right third premolars to create the traumatic occlusion. After 3, 7, 15, 30 experimental days, the specimens were examined under electron microscope. The following results were observed: 1. In 3, 7, 15 day groups, swelling of the mitochondria in the fibroblast was observed. 2. In 7, 14, day groups, dilation of the endoplasmicreticulum in the fibroblast was observed. 3. There were no remarkable changes in the nucleus of the fibroblasts and collagen fibers. 4. In 30 day group, the changes of the mitochondria and the ER in the fibroblast were recovered to have the similar appearance with that of the control group.

• Applied Microscopy
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• v.24 no.1
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• pp.77-85
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• 1994

In order to investigating the pulmonary toxicity of the O-chlorobenzyledene malononitrile (CS), lacrimating agent, $2.6g/m^3$ of CS was inhalated to Sprague-Dawley rats in the plastic chamber for 20 minutes. The ultrastructural changes of type II pneumocytes in the lung were observed with Hitachi 600 transmission electron microscope. The results obtained were as follows: 1. 3 hours after exposure to CS the fusion of surface microvilli, dilatation of cristernae of the rough endoplasmic reticulum, atrophy of Golgi complex and condensation, deletion of lamellated membranes in lamellar bodies were observed in type II pneumocytes. 2. One and 2 days after CS-exposure, disorganization of mitochondrial double membranes, fragmentations of rough endoplasmic reticulum were found in the great alveolar cells. In addition, decrease in amount of polyribosome granules and deletion or condensation of lamellated membranes in lamellar bodies were also observed. 3. 4 days after exposure to CS, the type II pneumocyte revealed new whorled lamellar membranes in lamellar bodies, a few intact rough endoplasmic reticulum and restoration of polyribosome granules. It is consequently suggested that CS induces degenerative changes of cytoplasmic organelles in the type II pneumocytes.

• Korean Journal of Veterinary Research
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• v.22 no.2
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• pp.99-109
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• 1982

Morphological changes of the oviductal epithelium of the rat treated with hormones ($17{\beta}$-estradiol ${\mu}g$/day and progesterone 2.5mg/day) for ten days were observed transmission and scanning electron microscopically. The results obtained were as follows: 1. The cilia formation of ciliary cell(CC) was more accelerated by the treatment of estradiol than progesterone, but the balance of estrogen and progesterone was required for the maintenance of CC. The effect of hormone was different between the segments for the maintenance of CC. 2. The short secretory cell(SSC) was severely inhibited in the formation of secretory granules with single hormonal treatment but the activity of secretion was more inhibited by progesterone than by estradiol. 3. The long secretory cell(LSC) had not a great difference between estradiol and progesterone treatments as compared with the normal sexual cycle, but the formation of secretory granules was somewhat accelerated by progesterone treatment. 4. The formation of secretory granules of junctional cell (JC) was severely accelerated by estradiol treatment as compared with the normal sexual cycle. The formation of secretory granules during progesterone treatment, on the other hand, was inhibited completely, but the numbers of pinocytotic vesicles appeared at the cytoplasmic apical portion. 5. Three types of secretory cells, SSC, LSC and JC, on the rat oviductal epithelium could be suggested to have different cell tapes respectively from the morphological changes by hormone treatment.

• Korean Journal of Bronchoesophagology
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• v.4 no.2
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• pp.182-187
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• 1998

Platelet activating factor (PAP) has been known as implicating as one of potent inflammatory mediators and reported 0 be involved in inflammation and allergy. PAF induces ciliary dysfunction and epithelial damage of human paranasal sinus mucosa in vitro. However, several recent papers have reported that PAF may not readily damage the airway epithelium. The aim of this study was to investigate the ultrastructural evidence to elucidate the pathogenesis of epithelial damage induced by PAF. Sixteen $\mu\textrm{g}$ g of PAF was applied into the maxillary sinuses of 6 rabbits. Rabbits were divided into 2 subgroups along with time interval at 1st and 3rd experimental day, and sinus mucosae were taken for the histopathologic study using electron microscopy. At 1st day, epithelial cells showed no ultrastructural change. Ultrastructures of the cilia were well preserved. Subepithelial space showed no evidence of the infiltration of inflammatory cells. Intravascular platelet aggregation and swelling of endothelial cells were evident. At 3rd day, epithelial cells showed vacuolar degeneration. Fusion of cilia forming giant cilia and focal loss of cilia were evident. Eosinophils were infiltrated in subepithelial and intraepithelial space. Swelling of endothelial cells, and migration of inflammatory cells into the connective tissue were evident. This study implies that epithelial damage induced by PAF may be secondary to the cytotoxicity of mobilized eosinophils rather than direct cytotoxicity of PAF.

• Journal of Oral Medicine and Pain
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• v.24 no.4
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• pp.397-410
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• 1999

In order to obtain the basic data concerning the optimal parameters in using Nd:YAG laser as a therapeutic modality to dentinal hypersensitivity, the author prepared 3 sections of sound dentin and 10 sections of sclerotic dentin with thickness of $0.5mm{\pm}0.1mm$ from human extracted teeth of anteriors and premolars, and applied the laser energy from a fiberoptic delivered, free running, pulsed Nd:YAG laser (wavelength 1064nm, pulse duration $120{\mu}sec$, fiber diameter $320{\mu}m$) to surfaces of sound and sclerotic dentin sections for 1 second with contact/unidirectional moving mode of the fiber under speed of 3mm~4mm/sec and parameters of 0.5W/10Hz, 1.0W/10Hz, 1.5W/10Hz, 2.0W/10Hz: $62J/cm^2$, $124J/cm^2$, $187J/cm^2$, $249J/cm^2$. The author comparatively evaluated the characteristics of ultrastructural changes on surfaces of sound and sclerotic dentin sections irradiated by the pulsed Nd:YAG laser using the scanning electron microscopy. A fairly ill-defined bordered surface of partially closed and melted dentinal tubules can be seen on the scanning electron microscopic feature of the sound dentin surface irradiated by the pulsed Nd:YAG laser with energy density of $62J/cm^2$. The physical modification of sound dentin surface extensively occurred depended on the increase of energy density from $62J/cm^2$ to $124J/cm^2$, $187J/cm^2$, $249J/cm^2$. While, a fairly well-defined bordered surface of partially closed and melted dentinal tubules with thickened peritubular dentin can be seen on the scanning electron microscopic feature of the sclerotic dentin surface irradiated by the pulsed Nd:YAG laser with energy density of $62J/cm^2$. The physical modification of sclerotic dentin surface of a fairly rough, shallow depression with many cracks, thickened peritubular dentin and structureless dentinal tubules extensively occurred depended on the increase of energy density from $62J/cm^2$ to $124J/cm^2$, $187J/cm^2$, $249J/cm^2$ compared to those of sound dentin surface irradiated by the pulsed Nd:YAG laser under the same parameters. Therefore, it is recommended that the pulsed Nd:YAG laser as a therapeutic modality to dentinal hypersensitivity should be applied with the less energy density than $62J/cm^2$ on the sound dentin surface, and its energy density on the partially sclerotic dentin surface should be lower than that on the sound dentin surface to preserve tooth from unnecessary excessive structural destruction.