• Title/Summary/Keyword: Electron donating

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Physiological Activities of Agaricus bisporus Extracts as Affected by Solvents (용매에 따른 양송이 추출물의 생리활성 효과)

  • Lim, Tae-Soo;Do, Jeong-Ryong;Kwon, O-Jun;Kim, Hyun-Ku
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.36 no.4
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    • pp.383-388
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    • 2007
  • Physiological activities of Agaricus bisporus extracts were examined. Nitrite-scavenging activity, superoxide dismutase (SOD)-like activity and electron-donating ability (EDA) of Agaricus bisporus extracted with water, 50%, and 100% ethanol were measured. Nitrite- scavenging activities at pH 1.2 were the most effective in water and 50% ethanol extracts from brown and white Agaricus bisporus samples. SOD-like activities of water and 50% ethanol extracts of both samples were $19.02{\sim}55.42%$ lower than those of 1.0% and 0.1% L-ascorbate solutions. SOD-like activities of brown Agaricus bisporus extracts were higher than those of white Agaricus bisporus extracts, and water extracts of the samples were the highest. EDAs of brown Agaricus bisporus extracts were higher $(40.35{\sim}66.54%)$ than those of white Agaricus bisporus extracts, while those of both extracts were lower than 1.0% and 0.1% L-ascorbate solutions. The results will be useful for understanding the physiological activities of Agaricus bisporus extracts.

Growth Chracteristics, Vitamin C Content and Antioxidative Activity among Local Strains of Sedum sarmentosum (돌나물 수집종간 생육특성, 비타민 C 함량 및 항산화 활성)

  • Kim, Hyo-Jin;Jung, Seung-Ho;Bae, Jong-Hyang;Lee, Seung-Yeob
    • Journal of Bio-Environment Control
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    • v.17 no.2
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    • pp.110-115
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    • 2008
  • To evaluate growth chracteristics, vitamin C content and antioxidative activity of Sedum sarmentosum, 13 local strains were cultivated in the field. Keumsan, Bucheon, and Pocheon strains had narrow and oblong leaf, long hight, and slender stem compared to the other strains. Wanju and Gunsan strains were not formed flower stalks, and did not flower during the flowering time. Vitamin C contents were ranged from 407.8 to $434.4\;mg{\cdot}kg^{-1}$, and it was high in Gwangyang, Buan, Wanju, and Gunsan strains. But vita min C contents didn't show a significant difference among strains. In antioxidative activity between fresh and freeze dried shoots, electron donating abilities (EDA) were ranged from 45.0 to 66.5% in MeOH extract of fresh shoot, and from 61.3 to 66.5% in MeOH extract of freeze dried shoot. Gwangyang, Suncheon, Wando strains showed high EDA compared to the others. Vitamine C content and antioxidant activity were highest in Gwangyang strain.

The Antioxidant Effect of Portulaca oleracea Extracts and Its Antimicrobial Activity on Helicobacter pylori (쇠비름 추출물의 항산화 효과 및 Helicobacterpylori에 대한 항균 활성)

  • Park, So-Hae;Kim, Dae-Kwang;Bae, Ji-Hyun
    • The Korean Journal of Food And Nutrition
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    • v.24 no.3
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    • pp.306-311
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    • 2011
  • This study was performed to investigate the effect of Portulaca oleracea extract on the antioxidant and antimicrobial activities against Helicobacter pylori. The each solvent extracts prepared from Portulaca oleracea were investigated by measuring total phenolic compounds, electron donating ability, superoxide dismutase-like ability and thiobarbituric acid reactive substances The herb extractor extract yielded the highest content of total phenolic compounds(72.2 mg%). The electron donating abilities(EDA) of ethyl acetate and methanol extracts showed high antioxidant activity. The superoxide dismutase (SOD)-like abilities of ethyl acetate and petroleum ether extracts also showed some activity. The antioxidant activity of thiobarbituric acid reactive substances was not significant. The petroleum ether extract of Portulaca oleracea showed the highest antimicrobial activity at 10,000 ppm concentration.

Quality Characteristics of Maejakgwa with Added Herb Extracts (허브 추출물에 따른 매작과의 품질 특성)

  • Kim, Kyoung-Suk;Choi, Sun-Young
    • The Korean Journal of Food And Nutrition
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    • v.21 no.3
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    • pp.312-319
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    • 2008
  • The principal objective of was to assess the effects of herb extracts on the antioxidant activity and quality characteristics of Maejakgwa. The total phenolic and flavonoid contents were found to be the highest by a significant degree in the rosemary, lavender, thyme, lemon balm and olive samples, in order. With regard to electron donating ability, increasing concentrations of added herbs resulted in a more significant rise in the DPPH radical scavenging effect in a range of concentrations of between $100{\sim}1000{\mu}g/m{\ell}$. In particular, the rosemary, lavender, and thyme samples were shown to be lower in antioxidant activity than ascorbic acid, but higher than a-tocopherol, thus the antioxidant activities detected in the rosemary, lavender, and thyme samples were considered to be excellent. With regard to reducing ability, increases in the concentration of added herb extract resulted in significant growth. However, the anti-oxidative activity detected in the rosemary extract was indicated to be superior even to that of $\alpha$-tocopherol when added to concentrations of $500{\sim}1,000{\mu}g/m{\ell}$. In our assessment of external appearance, color, flavor, texture, and overall quality, the highest preference was seen in the control group and in the group with 2% added rosemary extract. The lowest score was earned in the group with a 5% addition of rosemary. The functional ingredients and antioxidant activities of the samples were excellent. Our results appear to indicate that rosemary has some value as a natural antioxidant that can prevent oxidation in food.

Antibacterial and Antioxidative Activities of Various Extracts from Basidiomycetes (담자균 추출물의 항균작용 및 항산화작용)

  • 김선희;박찬성
    • Food Science and Preservation
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    • v.8 no.1
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    • pp.118-124
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    • 2001
  • The water and methanol extract were obtained from fruit body of 8 kind of edible mushrooms. The antibacterial activity of extracts on the growth of pathogenic bacteria(Listeria monocytogenes, Staphylococcus aureus, Aeromonas hydrophila, Escherichia coli O 157:H7 and Salmonella typhimurium) was determined. Methanol fraction of Gyrophora esculenta showed excellent antibacterial activity alai t 5 strains of pathogenic bacteria. The 80% methanol extract of Gyrophora esculenta and Phelinus were fractionated with diethylether, chloroform, ethyl acetate, butanol and water. The diethylether, ethyl acetate and butanol fractiorl of Gyrophora esculenta had excellent antibacterial activity and ethyl acetate and butanol fraction of Phellinus linteus had weak antibacterial activity against 5 strains of pathogenic bacteria. Electron donating ability of each fraction of Gyrophora esculenta was increased in order of ethylacetate, chloroform, butanol, diethylether and water. Nitrite scavenging ability was observed in ethyl acetate fraction of Gyrophora esculenta and other fractions showed no activities.

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Antioxidative Action of Corni Fructus Aqueous Extract on Kidneys of Diabetic Mice

  • Kim, Hye-Jeong;Kim, Bae-Hwan;Kim, Young-Chul
    • Toxicological Research
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    • v.27 no.1
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    • pp.37-41
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    • 2011
  • This study investigated the antioxidative action of Corni Fructus aqueous extract on kidneys of diabetic mice. The electron donating abilities of Corni Fructus aqueous extract and its antioxidant activities (XO, SOD, CAT, GST, eNOS) in kidneys of C57BL/6 or db/db mice were evaluated. For in vivo study, seven week-old male mice were divided into normal control group (NC, C57BL/6 mice), diabetic control group (DC, db/db mice) and Corni Fructus (500 mg/kg/day for 8 weeks) treated diabetic group (DCF, db/db mice). The electron donating abilities of Corni Fructus aqueous extract exhibited 7%, 24.4%, and 42.7% at concentrations of 100, 500, and $1000\;{\mu}g/ml$, respectively. The activity of XO in the DCF group was significantly lower than the DC group by 35% (p < 0.05). The SOD activity was significantly higher in the DCF group than the DC group by 26% (p < 0.05). The activities of CAT and GST were lowered in the DCF group than the DC group by 26% (p < 0.05) and 7.6%, respectively. The mRNA expression of eNOS in kidneys was lower in the DCF group than the DC group by 24%. These results indicate that Corni Fructus reduced oxidation stress as evidenced by the restoration of the enzymatic antioxidative defense system in renal tissues of db/db mice. It is suggested that these antioxidative actions of Corni Fructus on renal tissues in db/db mice could contribute to its renoprotective effects on diabetic nephropathy.

Utilization of Biomass Resources(II) Analysis of Polyphenol Components and Antioxidative Activities from Chestnut Inner Bark (Biomass 자원의 활용(II) 율추로부터 폴리페놀 성분의 분석과 항산화활성)

  • Jo, Jong-Soo;Kim, Yun-geun
    • Journal of the Korean Wood Science and Technology
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    • v.33 no.6 s.134
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    • pp.71-78
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    • 2005
  • For the utilization of chestnut inner bark as forest biomass, this studies have been carried out cleary investigated about phenolic compounds from the diethyl ether solubles and ethyl acetate solubles of hot water extract from chestnut inner bark. 3 compound were isolated and their structures were identified by spectroscopic methods. They were known compounds, 3,5-dihydroxybenzoic acid, 3,4,5-trihydrixybenzoic acid and catechin. Electron donating ability (EDA) of 3 compounds were investigated. Gallic acid and catechin were found to higher EDA than control, ascorbic acid and $\alpha$-tocopherol in 10 ppm, and especially gallic acid has very higher EDA, 21 times than ascorbic acid. This gallic acid of chemical structure were substituted with free phenolic hydroxyl groups at meta and para position. It was supposed that phenolic hydroxyl groups in chemical structure was a EDA factor.

Optimization of Corni fructus Extracts by Response Surface Methodology (반응표면분석에 의한 산수유 추출물의 추출조건 최적화)

  • Lee, Hye-Jin;Do, Jeong-Ryong;Kwon, Joong-Ho;Kim, Hyun-Ku
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.41 no.3
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    • pp.390-395
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    • 2012
  • Response surface methodology was used to monitor the characteristics of Corni fructus. A maximum electron donating ability of 81.27% was obtained at 119.71 W of microwave power, 7.71% of ethanol concentration, and 4.21 min of extraction time. The maximum inhibitory effect on tyrosinase was 105.92% at 143.36 W of microwave power, 58.19% ethanol concentration, and 6.71 min of extraction time. The maximum superoxide dismutase like activity was 87.08% under the extraction conditions of 107.33 W of microwave power, 96.14% ethanol concentration, and 31.49 min of extraction time. The total polyphenol content showed a maximum of 475.86 mg% at 140.29 W of microwave power, 27.44% ethanol concentration, and 58.69 min of extraction time. Based on the superimposition of four-dimensional RSM data regarding the electron-donating ability, inhibitory effect on tyrosinase, superoxide dismutase like activity, and total polyphenol content, the optimum ranges of extraction conditions were found to be at 78~98 W of microwave power, 3~33% ethanol concentration, and 3.6~9 min of extraction time.

Physiological Activities of Cucurbita moschata Duch. Extracts with Different Extraction Conditions (추출조건에 따른 늙은 호박 추출물의 생리활성)

  • Lee, Hye-Jin;Do, Jeong-Ryong;Kwon, Joong-Ho;Kim, Hyun-Ku
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.39 no.2
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    • pp.165-171
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    • 2010
  • This study was to investigate physiological activities of Cucurbita moschata Duch. extracts by various extraction conditions. Electron donating ability of 50% ethanol extract was 52.37% at 50 mL/g, which was higher than those of 1.0% and 0.1% L-ascorbate solutions. SOD-like activities were the most effective in all of the samples at 50 mL/g, which were barely detectable. Total polyphenol contents were the highest at 50 mL/g for all fiber extracts. The nitrite scavenging effects were the most effective in pH 1.2 at more than 80%. Tyrosinase-inhibitory activities ranged at fewer than 19%. The results will be useful for understanding of the physiological activities of Cucurbita moschata Duch. extracts.

The Antioxidant Ability and Nitrite Scavenging Ability of Poria cocos (복령(Poria cocos) 균사체의 항산화성 및 아질산염 소거작용)

  • 김대곤;손동화;최웅규;조영석;김수민
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.31 no.6
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    • pp.1097-1101
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    • 2002
  • This study was conducted to investigate the efficacy of antioxidant ability and nitrite scavenging ability of Poria cocos Poria cocos mycelium and Poria cocos fermented with rice were extracted with water and ethanol for measurement of degree of fat acidification against fish oil. Lower TBARS values of Poria cocos mycelium and Poria cocos fermented with rice were shown compared with control. Iron chelating ability of Poria cocos mycelium against Fe$^{2+}$, Cu$^{2+}$ was higher than Poria cocos fermented with rice. Electron donating abilitv of water extract and ethanol extract of Poria cocos mycelium were 76.8% and 79.9%, respectively. Higher nitrite scavenging ability was shown at ethanol extract than water extract of Poria cocos mycelium.ium.