• Title/Summary/Keyword: Electron Flow

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Application of Flow Cytometry to Monitoring of Liposomal Restructuring Induced by Listeria monocytogenes

  • Kim, Hyung-Joo;Bennetto, H.-Peter;Halablab, Mahmoud-A.
    • Journal of Microbiology and Biotechnology
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    • v.14 no.5
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    • pp.1099-1102
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    • 2004
  • Liposomal restructuring induced by hemolytic Listeria monocytogenes was investigated by using flow cytometry. When added to calcein-entrapped liposomes, hemolytic, but not non-hemolytic, Listeria monocytogenes were able to induce reformation of vesicles. Such restructuring of liposomes was easily monitored by flow cytometry. Electron microscopy also indicated major changes in the challenged liposomal structures. The preliminary results described may offer a simple and fast method for monitoring liposomal restructuring and for differentiating between hemolytic and non-hemolytic bacteria.

Analysis of Inductively Coupled Plasma using Electrostatic Probe and Fluid Simulation (정전 탐침법과 유체 시뮬레이션을 이용한 유도결합 Ar 플라즈마의 특성 연구)

  • Cha, Ju-Hong;Lee, Ho-Jun
    • The Transactions of The Korean Institute of Electrical Engineers
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    • v.65 no.7
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    • pp.1211-1217
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    • 2016
  • Discharge characteristics of inductively coupled plasma were investigated by using electrostatic probe and fluid simulation. The Inductively Coupled Plasma source driven by 13.56 Mhz was prepared. The signal attenuation ratios of the electrostatic probe at first and second harmonic frequency was tuned in 13.56Mhz and 27.12Mhz respectively. Electron temperature, electron density, plasma potential, electron energy distribution function and electron energy probability function were investigated by using the electrostatic probe. Experiment results were compared with the fluid simulation results. Ar plasma fluid simulations including Navier-Stokes equations were calculated under the same experiment conditions, and the dependencies of plasma parameters on process parameters were well agreed with simulation results. Because of the reason that the more collision happens in high pressure condition, plasma potential and electron temperature got lower as the pressure was higher and the input power was higher, but Electron density was higher under the same condition. Due to the same reason, the electron energy distribution was widening as the pressure was lower. And the electron density was higher, as close to the gas inlet place. It was found that gas flow field significantly affect to spatial distribution of electron density and temperature.

Simulation of Capacitively Coupled RF Plasma; Effect of Secondary Electron Emission - Formation of Electron Shock Wave

  • Park, Seung-Kyu;Kim, Heon-Chang
    • Journal of the Semiconductor & Display Technology
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    • v.8 no.3
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    • pp.31-37
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    • 2009
  • This paper presents one and two dimensional simulation results with discontinuous features (shocks) of capacitively coupled rf plasmas. The model consists of the first two and three moments of the Boltzmann equation for the ion and electron fluids respectively, coupled to Poisson's equation for the self-consistent electric field. The local field and drift-diffusion approximations are not employed, and as a result the charged species conservation equations are hyperbolic in nature. Hyperbolic equations may develop discontinuous solutions even if their initial conditions are smooth. Indeed, in this work, secondary electron emission is shown to produce transient electron shock waves. These shocks form at the boundary between the cathodic sheath (CS) and the quasi-neutral (QN) bulk region. In the CS, the electrons emitted from the electrode are accelerated to supersonic velocities due to the large electric field. On the other hand, in the QN the electric field is not significant and electrons have small directed velocities. Therefore, at the transition between these regions, the electron fluid decelerates from a supersonic to a subsonic velocity in the direction of flow and a jump in the electron velocity develops. The presented numerical results are consistent with both experimental observations and kinetic simulations.

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Probing Polarization Modes of Ag Nanowires with Hot Electron Detection on $Au/TiO_2$ Nanodiodes

  • Lee, Young Keun;Lee, Jaemin;Lee, Hyosun;Lee, Jung-Yong;Park, Jeong Young
    • Proceedings of the Korean Vacuum Society Conference
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    • 2013.02a
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    • pp.225-225
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    • 2013
  • Nanostructured noble metals have been attractive for their unusual optical properties and are widely utilized for various purposes. The optical properties mainly originating from collective electron oscillation can assist direct energy conversion via surface plasmon resonances. Here, we investigated the effect of surface plasmons of silver nanowires on the generation of hot electrons. It is reported that the surface plasmons of silver nanowires exhibit longitudinal and transverse modes, depending on the aspect ratio of the nanowires. In order to measure the hot electron flow through the metallic nanowires, chemically modified Au/TiO2 Schottky diodes were employed as the electric contact. The silver nanowires were deposited on a Au metal layer via the spray method to control uniformity and the amount of silver nanowire deposited. We measured the hot electron flow generated by photon absorption on the silver nanowires deposited on the Au/TiO2 Schottky diodes. The incident photon-to-current efficiency was measured a function of the photon energy, revealing two polarization modes of siliver nanowires: transverse and longitudinal modes. UV-Vis spectra exhibited two polarization modes, which are also consistent with the photocurrent measurements. Good correlation between the IPCE and UV-vis measurements suggests that hot electron measurement on nanowires on nanodiodes is a useful way to reveal the intrinsic properties of surface plasmons of nanowires.

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Properties of Electron Carriers in the Process of Methanol Oxidation in a New Restricted Facultative Marine Methylotrophic Bacterium, Methylophaga sp. MP

  • Koh, Moon-Joo;Kim, Chun-Sung;Kim, Yun-A;Choi, Hack-Sun;Cho, Eun-Hee;Kim, Eung-Bin;Kim, Young-Min;Kim, Si-Wouk
    • Journal of Microbiology and Biotechnology
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    • v.12 no.3
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    • pp.476-482
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    • 2002
  • Methanol dehydrogenase (MDH) and c-type cytochromes from marine methanol-oxidizing bacterium, Methylophaga sp. MP, were purified and characterized. The native MDH had a molecular mass of 148 kDa and its isoelectric point was 5.5. Two c-type cytochromes, $c_L\;and\;c_H$, were found, and their isoelectric points were 3.4 and 8.0, respectively. The purified MDH had higher thermal stability than that of the other soil methylotrophic bacteria. The electron flow rate from MDH to cytochrome $c_L$was higher than that from MDH to cytochrome $c_H$, indicating that the physiological primary electron acceptor for MDH is cytochrome $c_L$. The electron transfer from MDH to phenazine ethosulfate (PES, artificial electron acceptor) in the two dye (PES/DCPIP)-linked assay system was not inhibited by NaCl, whereas the electron flow from MDH to cytochrome $c_L$ in the cytochrome/DCPIP-linked assay system was suppressed significantly by NaCl. Metal chelating agents such as EDTA showed the same effects on the MDH activity.

Removal of Nutrients from Domestic Wastewater Using Intermittently Aerated Activated Sludge Systems Supplemented with Fermented Settled Sludge (발효된 1차 침전슬러지를 공급하여 간헐폭기조를 이용한 도시하수의 영양염류 처리)

  • Weon, Seung-Yeon;Lee, Sang-Ill
    • Journal of Korean Society on Water Environment
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    • v.20 no.1
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    • pp.18-23
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    • 2004
  • In this research, a 2-stage intermittently aerated activated sludge system(IA) and intermittently aerated dynamic flow activated sludge system(DF) were investigated for the removal of nutrients in domestic wastewater. Wastewater was characterized by low C/N( organics/nitrogen) ratio. $COD_{cr}$, $BOD_s$, TKN and TP concentrations of domestic wastewater were 235, 47, 32 and 5.4 mg/L, respectively. Three sets of IA and one set of DF were operated. Three of four systems were added with fermented settled sludge taken from primary settling tank as an external electron donor and the other(IA) for control reactor was operated without addition of electron donor. All systems were operated at same sludge retention time of 20 days and hydraulic retention time of 12hrs. The supplemental electron donor was supplied into the anoxic mode. A higher denitrification rate was observed from the reactors with fermented settled sludge as an electron donor for denitrification compared to that of without addition of organic source. The result of this study indicates that the settled primary sludge, if the fermented at the acid stage, was an excellent electron donor for denitrification. 81 % of TN and 80% of TP were removed from the systems with the supplemental organic source added. However, the control reactor without addition of electron donor showed only 39% of TN and 43% of TP.

Electron Beam Evaporated ITO Transparent Electrode for Highly Efficiency GaN-based Light Emitting Diode (고효율 질화갈륨계 발광 다이오드용 전자선 증착 ITO 투명 전도 전극 연구)

  • Seo, Jae Won;Oh, Hwa Sub;Kang, Ki Man;Moon, Seong Min;Kwak, Joon Seop;Lee, Kuk Hwe;Lee, Woo Hyun;Park, Young Ho;Park, Hae Sung
    • Korean Journal of Metals and Materials
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    • v.46 no.10
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    • pp.683-690
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    • 2008
  • In order to develop transparent electrodes for high efficiency GaN-based light emitting diodes (LEDs), the electrical and optical properties of the electron beam evaporated ITO contacts have been investigated as a function of the deposition temperature and flow rate of oxygen during the deposition. As the deposition temperature increases from $140^{\circ}C$ to $220^{\circ}C$, the resistivity of the ITO films decreases slightly from $4.0{\times}10^{-4}{\Omega}cm$ to $3.3{\times}10^{-4}{\Omega}cm$, meanwhile the transmittance of the ITO films significantly increases from 67% to 88% at the wavelength of 470 nm. When the flow rate of oxygen during the deposition increases from 2 sccm to 4 sccm, the resistivity of the ITO films increases from $3.6{\times}10^{-4}{\Omega}cm$ to $7.4{\times}10^{-4}{\Omega}cm$, meanwhile the transmittance of the ITO films increases from 86% to 99% at 470 nm. Blue LEDs fabricated with the electron beam evaporated ITO electrode show that the ITO films deposited at $200^{\circ}C$ and 3 sccm of the oxygen flow rate give a low forward-bias voltage of 3.55 V at injection current of 20 mA with a highest output power.

Analysis of electron emission mechanism in surface conduction electron emission displays (표면전도 전자방출 표시장치의 전자방출 구조해석)

  • 김영삼;김영권;오현주;조대근;길도현;김대일;강준길;강승언;최은하
    • Journal of the Korean Vacuum Society
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    • v.8 no.4A
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    • pp.410-416
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    • 1999
  • It is confirmed that the cause of anode current in SEDs (surface conduction electron emission displays) is the inertial force of electron emitted from the cathode surface in the calculation of electron trajectory. In the fissure of sub-micron, most of electrons emitted from the area of the cathode edge flow into the coplanar anode, while some electrons are emitted into the display surface by the current ratio of $10^{-3}$. The later electrons are forced to fly into the display surface by the centrifugal force due to the curved electric field between top side surfaces near the fissure.

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Excitation Temperature and Electron Number Density Measured for End-On-View Indectively Coupled Plasma Discharge

  • Nam, Sang Ho;Kim, Yeong Jo
    • Bulletin of the Korean Chemical Society
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    • v.22 no.8
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    • pp.827-832
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    • 2001
  • The excitation temperature and electron number density have been measured for end-on-view ICP discharge. In this work, end-on-view ICP-AES equipped with the newly developed “optical plasma interface (OPI)” was used to eliminate or remove the neg ative effects caused by end-on-plasma source. The axial excitation temperature was measured using analyte (Fe I) emission line data obtained with end-on-view ICP-AES. The axial electron number density was calculated by Saha-Eggert ionization equilibrium theory. In the present study, the effects of forward power, nebulizer gas flow rate and the presence of Na on the excitation temperature and electron number density have been investigated. For sample introduction, two kinds of nebulizers (pneumatic and ultrasonic nebulizer) were utilized.

Effect of Joule Heating Variation on Phonon Heat Flow in Thin Film Transistor (줄 가열 변화에 따른 박막 트랜지스터 내 포논 열 흐름에 대한 수치적 연구)

  • Jin, Jae-Sik;Lee, Joon-Sik
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.33 no.10
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    • pp.820-826
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    • 2009
  • The anisotropic phonon conductions with varying Joule heating rate of the silicon film in Silicon-on-Insulator devices are examined using the electron-phonon interaction model. It is found that the phonon heat transfer rate at each boundary of Si-layer has a strong dependence on the heating power rate. And the phonon flow decreases when the temperature gradient has a sharp change within extremely short length scales such as phonon mean free path. Thus the heat generated in the hot spot region is removed primarily by heat conduction through Si-layer at the higher Joule heating level and the phonon nonlocality is mainly attributed to lower group velocity phonons as remarkably dissimilar to the case of electrons in laser heated plasmas. To validate these observations the modified phonon nonlocal model considering complete phonon dispersion relations is introduced as a correct form of the conventional theory. We also reveal that the relation between the phonon heat deposition time from the hot spot region and the relaxation time in Si-layer can be used to estimate the intrinsic thermal resistance in the parallel heat flow direction as Joule heating level varies.