• Title/Summary/Keyword: Electroactive bacteria

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Enhanced Current Production by Electroactive Biofilm of Sulfate-Reducing Bacteria in the Microbial Fuel Cell

  • Eaktasang, Numfon;Kang, Christina S.;Ryu, Song Jung;Suma, Yanasinee;Kim, Han S.
    • Environmental Engineering Research
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    • v.18 no.4
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    • pp.277-281
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    • 2013
  • A dual-chamber microbial fuel cell (MFC) inoculated with Desulfovibrio desulfuricans and supplemented with lactate as an organic fuel was employed in this study. Biofilm formed on the anodic electrode was examined by scanning electron microscopy, revealing that the amount of biofilm was increased with repeated cycles of MFC operation. The maximum current production was notably increased from the first cycle ($1,310.0{\pm}22.3mA/m^2$) to the final cycle ($1,539.4{\pm}25.8mA/m^2$) of MFC run. Coulombic efficiency was also increased from $89.4%{\pm}0.2%$ to $98.9%{\pm}0.5%$. We suggest that the current production efficiency was related to the biomass of biofilm formed on the electrode, which was also increased as the MFC run was repeated. It was also found that D. desulfuricans, which colonized on the electrode, produced filaments or nano-pili. Nano-pili were effective for the attachment of cells on the electrode. In addition, the nano-pili provided a cell-to-cell link and stimulated the development of thicker electroactive biofilm, and therefore, they facilitated electron transfer to the anode. Conclusively, the biofilm of D. desulfuricans enhanced the current production in the MFC as a result of effective attachment of cells and electron transfer from the cell network to the electrode.

Nanogap-Based Electrochemical Detection of Protein, Virus, and Bacteria

  • Park, Dae Keun;Kim, Soohyun;Yun, Kum-Hee;Pyo, Hanna;Kang, Aeyeon;Kim, Daehee;Lee, Cho Yeon;Yun, Wan Soo
    • Proceedings of the Korean Vacuum Society Conference
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    • 2016.02a
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    • pp.353.2-353.2
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    • 2016
  • We studied electrochemical detection of Botulinum neurotoxin, Vaccinia virus, and Streptococcus Pneumoniae based on nanogap device. Target bio substances were employed as representative targets of protein, virus, and bacteria, respectively. Redox current generated by ferri/ferrocyanide as an electroactive probe was enhanced according to gap distance which was controlled by surface-catalyzed chemical deposition. We found that enhanced electrochemical signal leads more sensitive signal changes according to selective interaction of target and its complementary elements on the electrode or gap area. In case of Botulinum neurotoxin, the redox signal showed a time-dependent increase due to cleavage of the immobilized peptide which blocked redox cycling. Redox cycling was also hindered by Vaccinia virus and Streptococcus Pneumoniae which were selectively immobilized in the gap area.

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Menadione-Modified Anodes for Power Enhancement in Single Chamber Microbial Fuel Cells

  • Ahmed, Jalal;Kim, Sunghyun
    • Bulletin of the Korean Chemical Society
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    • v.34 no.12
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    • pp.3649-3653
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    • 2013
  • As anode fabrication with different materials has been proven to be a successful alternative for enhancing power generation in the microbial fuel cells, a new approach to improved performance of MFCs with the use of menadione/carbon powder composite-modified carbon cloth anode has been explored in this study. Menadione has formal potential to easily accept electrons from the outer membrane cytochromes of electroactive bacteria that can directly interact with the solid surface. Surface bound menadione was able to maintain an electrical wiring with the trans-membrane electron transfer pathways to facilitate extracellular electron transfer to the electrode. In a single chamber air cathode MFC inoculated with aerobic sludge, maximum power density of $1250{\pm}35mWm^{-2}$ was achieved, which was 25% higher than that of an unmodified anode. The observed high power density and improved coulomb efficiency of 61% were ascribed to the efficient electron shuttling via the immobilized menadione.

Trends of microbial electrochemical technologies for nitrogen removal in wastewater treatment (하폐수처리에서 질소 제거를 위한 미생물 전기화학 기술의 동향)

  • Chai, Hyungwon;Choi, Yonghoon;Kim, Myeongwoon;Kim, Youngjin;Jung, Sokhee P.
    • Journal of Korean Society of Water and Wastewater
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    • v.34 no.5
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    • pp.345-356
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    • 2020
  • The removal of organic carbon and nutrients (i.e. N and P) from wastewater is essential for the protection of the water environment. Especially, nitrogen compounds cause eutrophication in the water environment, resulting in bad water quality. Conventional nitrogen removal systems require high aeration costs and additional organic carbon. Microbial electrochemical system (MES) is a sustainable environmental system that treats wastewater and produces energy or valuable chemicals by using microbial electrochemical reaction. Innovative and cost-effective nitrogen removal is feasible by using MESs and increasing attention has been given to the MES development. In this review, recent trends of MESs for nitrogen removal and their mechanism were conclusively reviewed and future research outlooks were also introduced.