• Plant Biotechnology Reports
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• 제3권3호
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• pp.259-266
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• 2009

A plantlet regeneration protocol was developed on pot-grown mature plants of Elaeocarpus robustus Roxb. cv. Dwarf from nodal and leaf explants. The best yield of adventitious shoots was achieved from the leaf-derived calli in a modified MS ($MMS_1$, half strength of major salts, full strength of minor salts, and vitamins) medium containing $4.0{\mu}M$ BA + $4.0{\mu}M$ Kn + $0.5{\mu}M$ NAA + 15% coconut water (CW). The shoot multiplication rate was amplified about twofold per culture after the addition of 15% CW to the medium. The rate of shoot multiplication reached maximum at the 5th subculture, and it maintained this rate throughout the 3 subsequent subcultures. The best rooting in vitro was investigated by subculturing the microcuttings in an $MMS_2$ (half strength of both major salts and minor salts and full strength of vitamins) medium containing $1.0{\mu}M$ IBA in the dark for one initial week at $30^{\circ}C$, followed by subculturing them in a plant-growth regulator (PGR)-free medium in the light. The plantlets raised in vitro were successfully established under ex vitro conditions.

• Food Science and Biotechnology
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• 제17권4호
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• pp.718-722
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• 2008

Elaeocarpus sylvestris var. ellipticus (E.S.), which contains 1, 2, 3, 4, 6-penta-O-galloyl-beta-D-glucose (PGG), is reported to have the ability to scavenge oxygen radicals, thereby protecting rat neuronal cells from oxidative damage. The potential of an E.S. extract, which contains a rich PGG, to protect radiosensitive lymphocytes and intestinal crypt cells from radiation injury induced by a single whole-body irradiation (WBI) in vivo was investigated. Our results demonstrated that in immune cells, E.S. treatment decreased the percent of tail DNA, a parameter of DNA damage, compared with levels in untreated, irradiated controls. Furthermore, apoptosis was significantly decreased in lymphocytes and intestinal crypt cells of E.S.-treated mice compared with irradiated controls. These results suggest that the E.S. extract can strengthen the radioresistance of radiosensitive lymphocytes and crypt cells by preventing apoptosis. Therefore, it was concluded that E.S. extract has the radioprotective effects in vivo through an inhibition of apoptosis.

• The Plant Pathology Journal
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• 제39권1호
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• pp.149-157
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• 2023

Phytoplasmas were discovered in diseased Elaeocarpus sylvestris trees growing on Jeju Island that showed symptoms of yellowing and darkening in the leaves. Leaf samples from 14 symptomatic plants in Jeju-si and Seogwipo-si were collected and phytoplasma 16S rRNA was successfully amplified by nested polymerase chain reaction using universal primers. The sequence analysis detected two phytoplasmas, which showed 99.5% identity to 'Candidatus Phytoplasma asteris' and 'Ca. P. malaysianum' affiliated to 16SrI and 16SrXXXII groups, respectively. Through polymerase chain reaction-restriction fragment length polymorphism (RFLP) analyses using the AfaI (RsaI) restriction enzyme, the presence of two phytoplasmas strains as well as cases of mixed infection of these strains was detected. In a virtual RFLP analysis with 17 restriction enzymes, the 16S rRNA sequence of the 'Ca. P. asteris' strain was found to match the pattern of the 16SrI-B subgroup. In addition, the phytoplasmas in the mixed-infection cases could be distinguished using specific primer sets. In conclusion, this study confirmed mixed infection of two phytoplasmas in one E. sylvestris plant, and also the presence of two phytoplasmas (of the 16SrI and 16SrXXXII groups) in Jeju Island (Republic of Korea).

• 대한화장품학회지
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• 제50권1호
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• pp.49-58
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• 2024

담팔수는 괭이밥목 담팔수과의 상록교목으로 추위에 약하여 아열대 지역에서만 자생하는 식물이다. 담팔수는 flavonoid, coumarin, polyphenol 성분이 보고되어져 있으며, 해당 성분들에 의하여 항균 및 항산화 효능들이 있는 것으로 보고되어져 있다. 본 연구에서는 담팔수의 생리활성을 높이기 위하여 로스팅 기법을 도입하였으며, 로스팅한 담팔수 추출물 및 분획물에 대한 성분 변화를 확인한 후 항산화, 총 페놀 및 총 플라보노이드 함량 측정을 진행하였다. 로스팅 전 후 추출물 분석을 진행하여 총 4 개 성분(brevifolin, ellagic acid, quercetin, kaempferol)의 함량이 증가되는 것을 확인하였으며, 로스팅을 한 추출물에서 보다 나은 항산화 효능을 나타내는 것을 확인하였다. 최적 로스팅 조건은 200 ℃에서 30 min 로스팅 처리를 하였을 때 항산화 효능이 가장 뛰어난 것으로 확인하였으며, 총 페놀 및 총 플라보노이드 함량 또한 가장 우수한 것으로 확인하였다. 최적 로스팅 조건으로 제조한 담팔수 추출물에서 향상된 항산화 효능을 확인하였으며, 향후 화장품 및 식품 소재로 활용이 가능할 것으로 사료된다.

• 한국조경학회지
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• 제25권3호
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• pp.210-221
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• 1997

This study was conducted to investigte the visual preference and response between introduced and native trees for utilization of the south native trees in interior space. 1. The intensity of illumination was below 500lux at 24 buildings of interior spaces surveyed. Generally the temperature was 24~$26^{\circ}C$, and humidity was 60~70%. 2. Total number of introduced tree species were 9 familiar, 16 genus, 18 species, especially palm genus were the largest tree among them at these surveyed interior spaces, but native trees were only 2 species. The introduced tree of 2 to 3m in height was 46 percent, and the case of 1.2 to 2m was 42 percent. 3. The mean of visual preference was the highest at Rhapis excelsa and howeia belmoreana, and followed with Neolitsea sericea, Daphniphllum macropodum, Listsea japonicum. In the mean of visual preference for selected tree groups, native tree was not different as compared with introduced tree. 4. Visual image factor of the native and introduced trees at interior spaces was classified by individual factor and emotional factor. These 2 factors were shown 66.5% total variance. The native tree was strongly recognized on individual factor, as , and introduced tree was also strongly recognized on emotional factor. 5. In the analysis of visual preference and image, a difference between the selected groups of the introduced and native trees were not shown significant, and the introduced trees were similar to the native trees on the visual image. This result was as follow There was an analogy between Cinnamomum camphora, Ligustrum japonicum and Ficus retusa, Ficus benjamina. There is an analogy between Castanopsis cuspidata var. sieboldii, Cinnamomum japonicum and Ficus retusa, Ficus benjamina. There was an analogy between Elaeocarpus sylvestris var. ellipticus, Fatsia japonica and Rhapis excelsa, Howeia belmoreana. There was an analogy between Neolitsea sericea, Daphniphyllum macropo여, Listsea japonica and Rhapis excelsa, Howeia belmoreana. There was an analogy between Elaeocarpus sylvestris var. ellipticus, Fatsia japonica and Dracaena fragranse, Ficus elastica, Monstera deliciosa.

• Journal of Forest and Environmental Science
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• 제11권1호
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• pp.40-49
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• 1995

담팔수짝지벌레(신청)는 자충(雌蟲)만이 존재하는 산자성 단위생식(單位生殖)을 하며 부화(孵化)한 유충(幼蟲)은 3령을 경과하여 성충(成蟲)이된다. 본(本) 짝지벌레는 1년(年)에 2세대(世代)경과하고 제(第) 1세대(世代) 분산유충(分散幼蟲)은 5월초순(月初旬)부터 6월중순(月中旬)까지, 제(第) 2세대(世代)는 8월상순(月上旬)부터 10월상순(月上旬)까지 발생하였으며 최성기는 발생초기에 나타났다. 1령(領) 분산유충(分散幼蟲)은 10시(時)부터 14시(時)사이에 가장 많이 성충체(成蟲體) 복부(腹部)로부터 이탈하였으며 분산한 1령유충(齡幼蟲)은 8시간(時間)이내에 90% 이상의 개체(個體)가 담팔수 잎에 정착하였다. 본(本) 종(種)은 담팔수 잎 양면(養面)에 주로 분포(分布)하였으며 수간별(樹間別)의 개체군(個體群) 밀도(密度)의 분포차(分布差)가 크게 나타났다. 성충기의 경우 수관하부(樹冠下部)의 밀도(密度)가 높았으며 방향간(方向間)의 밀도차(密度差)는 나타나지 않았다. 엽단위(葉單位)의 분포(分布)는 약한 colony성(性)을 갖는 집중분포형(集中分布型)으로 판명(判明)되었다.

• Journal of Plant Biotechnology
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• 제47권1호
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• pp.90-99
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• 2020

This study aimed to screen for plants with antimicrobial potential among the evergreen woody species of South Korea that are used for horticulture and landscaping and to provide basic information about plants with proven antimicrobial activity to underpin future research. The plant materials were extracted under various conditions, and the antimicrobial activities of the extracts were evaluated by agar diffusion assay. The screening tests demonstrated that the crude extracts of 43 species had inhibitory effects against S. aureus. The inhibitory activities of four species (Elaeocarpus sylvestris, Camellia japonica, Cleyera japonica, and Quercus salicina) were relatively higher than that of the synthetic antimicrobial agents methylparaben and phenoxyethanol. The highest inhibitory activity was observed with the leaf extracts (extracted with methanol for 30 minutes) of E. sylvestris, based on induction of the largest inhibition zone of 23.3 mm in size. In addition, solvent fractions of E. sylvestris were evaluated. The largest inhibitory zone of 23.1 mm was observed for the n-butanol fraction, which is likely to contain effective compounds that exhibit inhibitory activity against S. aureus. In contrast, n-hexane and residual aqueous fractions showed no antimicrobial activity. Overall, our findings confirm that evergreen woody plants native to South Korea have potential antimicrobial activity.

• Forest Science and Technology
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• 제14권4호
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• pp.160-168
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• 2018

The seeds of Elaeocarpus serratus, a tropical underutilized fruit tree are characterized by hard seed coat and consequent poor water uptake and low germination. To improve the regeneration through seeds, various parameters such as viability of seeds, water uptake, and effect of seed mass on germination and pretreatments were performed using a completely randomized design (CRD). Tetrazolium (TZ) test was conducted using fresh, mature seeds revealed $50{\pm}2.56%$ mean viability. Seeds of different weight classes showed similar pattern of water uptake and the saturation level was achieved at 60 hrs of soaking. Seeds belong to weight class 2.6-3.5g were germinated ($12.5{\pm}1.26%$) with $175{\pm}1.75days$ (d) of mean time taken for germination (MTG). Germination capacity of seeds varied significantly among different populations and Varkala population gave $12.5{\pm}1.1%$ germination with $174.6{\pm}2.5d$ MTG. Among various seed treatments, mechanical scarification was superior in germination and significant reduction in MTG ($p{\leq}0.05$). The mechanical scarification by complete removal of seed coat resulted in $49.2{\pm}1.52%$ germination within a short period of time ($9.52{\pm}0.89d$ MTG). However, the complete removal of seed coat without damaging to embryo is a difficult task. An alternate treatment (Mechanical scarification II) by making cracks on nut faces vertically followed by soaking in distilled water for 24 hrs gave $48.4{\pm}1.73%$ germination with significantly reduced MTG ($12.14{\pm}0.56d$) over unsoaked, untreated control ($6.5{\pm}1.84%$ germination and $197.18{\pm}1.79d$ MTG; $p{\leq}0.05$). This treatment (Mechanical scarification II) is therefore recommended for E. serratus seeds as it can adopt easily and can achieve 7 fold increases in germination over control. The recorded germination through mechanical scarification is in tune with realized viability percentage of the seeds.

• 한국전통조경학회지
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• 제39권1호
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• pp.37-42
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• 2021

문섬은 국내 최대 담팔수 자생지로 알려져 있는 섬이다. 최근 문섬에서 담팔수군락의 병해피해가 보고되었다. 본 연구는 담팔수 최대 자생지인 문섬지역에 대해 담팔수 개체군의 피해상황과 생육특성을 파악하기 위하여 수행되었다. 담팔수 생존개체의 개체군 특성을 파악함으로써 군락의 자생적 재생가능성에 대해 평가하고, 자생지 복원 방안을 논의하고자 하였다. 문섬에 분포하는 담팔수 개체의 분포 현황을 파악하기 위하여 개체군 전수조사를 실시하였다. 현장조사는 개체의 GPS 위치정보와 함께 수고, 근원경, 흉고직경, 생육상태 등을 면밀히 기록하였다. 문섬에 분포하는 담팔수 개체의 생육상태와 각 개체들의 수고, 흉고직경, 근원경 특성을 분석하였다. 문섬에서 확인된 담팔수 총 개체군 수는 293본이었다. 2005년 문섬 육상생태 환경조사보고서에서 보고한 울창한 담팔수군락은 현재 크게 훼손되거나 피해를 입은 상태로 확인되었다. 특히 숲의 수관구조를 이루는 수고 6m이상의 성목에서 피해는 더욱 심각하여 성목의 80.6% 개체가 고사한 것으로 확인되었다. 근원경을 기준으로 숲의 건강성을 지표하는 장령목 생육특성에서도 75.4%가 고사하거나 생육상태가 불량한 것으로 확인되었다. 문섬의 담팔수 자생지 복원을 위하여 병해충 방제, 성목 보존, 유령목의 적정 간격유지 및 현지 외 이식 후 재도입 등의 보존노력이 필요한 것으로 판단된다.

• 한국자원식물학회지
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• 제15권2호
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• pp.114-122
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• 2002

우리나라 최남단 난대림지역인 천지연계곡에 분포하고 있는 목본식물의 종조성, 분포현황 및 관속식물상을 조사하였다. 천지연계곡의 교목성 수종은 29종이었으며 , 그 중 침엽수인 해송을 제외한 활엽수종 중 낙엽활엽수가 13종이었다. 교목성 수종은 2,395본이었으며, 그 중 낙엽활엽수가 1,427본으로 상록활엽수보다 많았다. 교목성 수종의 피도는 상록활엽수와 낙엽활엽수가 각각 42.3%와 48.7%로 낙엽활엽수의 상대피도가 다소 높았다. 관목성 수종은 37종이 분포하는 것으로 밝혀졌으며, 개체수에 있어서는 교목성 수종과 달리 상록활엽수종들의 개체수가 많았다. 관목성 수종의 상대피도는 상록활엽수와 낙엽활엽수가 각각 35.4%와 64.4%로 상록활엽수의 비가 현저히 높았다. 천지연에 분포하고 있는 관속식물은 114과 320속 397종 1아종 39변종 9품종으로 총446 분류군이었으며, 그 중 제주도 특산식물은 가시딸기(Rubus hongnoensis Nakai)와 제주상사화(Lycoris chejuensis K. Tae et S. Ko) 2 분류군, 우리나라에서는 제주도에만 분포하는 식물은 괴불이끼(Crepidomanes insigne (v.d. Bosch) Fu) 등 17 분류군, 천연기념물은 담팔수(Elaeocarpus sylvestris (Loureiro) Poiret var. ellipticus (Thunb.) Hara) 1 분류군, 법정보호식물은 솔잎란(Psilotum nudum (L.) Griseb.)과 고란초(Crypsinus hastatus (Thunb.) Copeland) 2 분류군, 귀화식물은 수영(Rumex acetosa L.) 등 38 분류군, 식재식물은 은행나무(Gingo biloba L.)등 75 분류군이었다.