• Food Science and Preservation
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• v.30 no.5
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• pp.797-810
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• 2023

Elaeagnus umbellata leaves have been reported to suppress inflammation, allergic responses, lung cancer proliferation and oral bacterial growth. Cadmium (Cd) is a heavy metal that has been found to cause many toxicities, including liver toxicity. The aim of this study was to evaluate the capacity of 70% ethanol extract of E. umbellata leaves (EUL) to protect human hepatocytes from Cd toxicity. After exposure of HepG2 cells to Cd at 10 𝜇M for 24 h, cell viability, expression levels of apoptosis- and antioxidant-related proteins, reactive oxygen species (ROS) accumulation and Cd uptake were assessed. EUL protected HepG2 cells from Cd-induced apoptosis as determined by MTT assay. A decrease in caspase-3 and p-p53 protein levels was observed in cells pretreated with EUL prior to Cd exposure. Furthermore, the Cd-induced increase in intracellular DCF fluorescence was attenuated by EUL, indicating that the Cd-induced apoptosis preventing effect was associated with the suppression of ROS accumulation. Moreover, EUL's effects on the inhibition of p38, JNK, and AKT phosphorylation also appear to be associated with protection against Cd toxicity. Moreover, EUL upregulated Cd-depressed expression of Nrf2, HO-1, catalase, and MT-1,2 proteins, suggesting that Cd uptake-induced apoptosis in HepG2 cells may be inhibited by EUL's antioxidative potential.

• Molecules and Cells
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• v.23 no.1
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• pp.115-121
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• 2007

Root nodule formation is controlled by plant hormones such as auxin. Auxin-repressed protein (ARP) genes have been identified in various plant species but their functions are not clear. We have isolated a full-length cDNA clone (EuNOD-ARP1) showing high sequence homology to previously identified ARP genes from root nodules of Elaeagnus umbellata. Genomic Southern hybridization showed that there are at least four ARP-related genes in the genome of E. umbellata. The cDNA clone encodes a polypeptide of 120 amino acid residues with no signal peptide or organelle-targeting signals, indicating that it is a cytosolic protein. Its cytosolic location was confirmed using Arabidopsis protoplasts expressing a EuNOD-ARP1:smGFP fusion protein. Northern hybridization showed that EuNOD-ARP1 expression was higher in root nodules than in leaves or uninoculated roots. Unlike the ARP genes of strawberry and black locust, which are negatively regulated by exogenous auxin, EuNOD-ARP1 expression is induced by auxin in leaf tissue of E. umbellata. In situ hybridization revealed that EuNOD-ARP1 is mainly expressed in the fixation zone of root nodules.

• Journal of the Korean Society of Food Science and Nutrition
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• v.45 no.6
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• pp.828-834
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• 2016

The aim of this study was to evaluate the physicochemical properties and antioxidant and anti-proliferative activities of different plant parts of Elaeagnus umbellata Thunb. extracted with ethanol (EtOH). EtOH extract of stems presenting the highest content of polyphenols showed the strongest 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity ($EC_{50}=54.04{\mu}g/mL$). The total content of free amino acids decreased in the order of leaves (6,179.12 mg/100 g)> stems (1,211.69 mg/100 g)> fruits EtOH extract (378.88 mg/100 g), and asparagine (1,907.57 mg/100 g), ${\gamma}-aminobutyric$ acid (300.17 mg/100 g), and proline (233.48 mg/100 g) were the major free amino acid in leaves, stems, and fruits, respectively. Five phenolic compounds in each extract were measured by using liquid chromatography- tandem mass spectrometry, and gallic acid (98.95 mg/100 g) and (+)-catechin (1,575.99 mg/100 g) were present as major components in leaves and stems, respectively. EtOH extract of leaves showed the highest anti-proliferative activity against HepG2 cells as measured by 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazoliumbromide and lactate dehydrogenase assay but had no effects on Chang liver cells.

• Journal of Environmental Science International
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• v.27 no.10
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• pp.875-881
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• 2018

To select fruit trees suitable for shaded urban garden with Elaeagnus umbellata var. coreana, Malus domestica 'Alps Otome', Malus domestica 'Fujii', Prunus mume, Prunus persica for. persica, Rubus fructicosus, and Vaccinium corymbosum 'Reka', leaf growth in response to different light was investigated two years after 35% shade treatment. Leaf area of E. umbellata var. coreana, M. domestica 'Alps Otome', P. mume, P. persica for. persica, and V. corymbosum 'Reka' increased in shading. Fresh weight of leaves make inconsistent response to shading in every species but dry weight of E. umbellata var. coreana and P. persica for. persica showed the highest 150% and 148%, increment, respectively. Althought leaf water content of E. umbellata var. coreana decreased in shading, there is no difference in P. persica for. persica. Chlorophyll value of E. umbellata var. coreana and P. persica for. persica that showed higher than any other species is correlated with dark leaf green. Compared to specific leaf weight of E. umbellata var. coreana, P. persica for. persica, and V. corymbosum 'Reka' showed lower than any other species in shading, that of M. domestica 'Fujii', and R. fructicosus increased in reverse. These results indicate that E. umbellata var. coreana and P. persica for. persica that showed high value in several invesetigaton items are suitable for shady urban condition considering leaf growth in response to shading.

• Journal of Food Hygiene and Safety
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• v.10 no.4
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• pp.219-224
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• 1995

The antimutagenic effects of 46 kinds of medicinal plants that have been used as traditional folk antitumor agents in Korea were studied by using Ames mutagenicity test. Most of the methanolic extracts from the plants which were used in this experiment showed strong antimutagenic activity toward aflatoxin B1(AFB1) in Salmonella typhimrium TA100 and TA98. However, N-methyl-N'-nitro-N-nitrosoguanidine(MNNG) induced mutagenicity was not blocked by adding the methanolic extracts of the plants except persimmon leaves (Diospyros kaki Thunberg)and Elaeagnus umbellata.

• Journal of Life Science
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• v.29 no.5
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• pp.555-563
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• 2019

This study was undertaken to investigate the effect of an ethanol extract of Elaeagnus umbellata leaves (EUL-EE) on skin-related biological activities. Previously, we have reported that gallic acid was the major phenolic compound in EUL-EE through quantitative analysis and that EUL-EE had an inhibitory effect against the proliferation of liver cancer HepG2 cells. In the present study, the inhibitory effects of EUL-EE on melanin production and tyrosinase activity in ${\alpha}$-melanocyte-stimulated hormone-stimulated B16-F0 cells were determined to assess the effects of EUL-EE on skin whitening. The anti-wrinkle effect using UVB-irradiated CCD-986sk cells was examined by the expression of type I procollagen and metalloproteinase (MMP)-1 release. The EUL-EE significantly decreased intracellular melanin production (33.0% inhibition at $100{\mu}g/ml$) when compared with untreated B16-F0 cells. Tyrosinase activities in the stimulated B16-F0 cells were also decreased by EUL-EE (47.8% inhibition at $100{\mu}g/ml$). The EUL-EE also dose-dependently increased the production of type I procollagen (up to 1.74-fold at $250{\mu}g/ml$) in CCD-986sk cells when compared with UVB-irradiated controls. EUL-EE showed no cytotoxicity at concentrations up to $500{\mu}g/ml$. In addition, EUL-EE at $10-500{\mu}g/ml$ inhibited the release of MMP-1 to the medium from UVB-irradiated CCD-986sk cells. Taken together, these observations indicate that EUL-EE has high potential for use as inner beauty and cosmetic materials due to its whitening and anti-wrinkle effects.

• Korean Journal of Environment and Ecology
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• v.26 no.1
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• pp.39-45
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• 2012

To explore on the defense strategies against hervivory of 67 broadleaved tree species, morphological characteristics of the leaf, leaf domatia structure and the number, herbivores insects and mites on the leaves, collected from the trees growing in Taean-gun, Chuncheongnam-do were investigated. 46 broadleaved tree species(68.7%) had the domatia structures, and 21 species including Quercus salicina and Magnolia grandiflora did not. 31 species including Juglans mandshurica and Carpinus laxiflora reveals tuft type, 12 species including Quercus dentata and Corylus heterophylla reveals pocket+tuft type, and 2 species, Sorbus alnifolia and Prunus yedoensis does pocket type, and Viburnum odoratissimum var. awabuki does pouch type. Domatia number per leaf proves the highest figures, 23.4/leaf for Quercus dentata. Plant defense strategies using leaf lower-surface trichomes of Magnolia grandiflora reveals dense villous, those of Populus alba and Vitis vinifera reveals dense pilose, that of Elaeagnus umbellata does dense scaly hairs, that of Pueraria lobata does dense strigose. Plant defense strategies using extrafloral nectaries were adapted 23 tree species(34.3%). Observed examples are Prunus tomentosa, Ficus carica, Viburnum dilatatum and Carpinus laxiflora. Predatory mites were observed on the leaves of 40 tree species(59.7%), and mean values of predatory mites was highest values 23.4/leaf in Quercus dentata. Minute arthropods destroying the leaf of broadleaved trees. are such as Periphyllus californiensis, P. viridis, Diaspididae sp., gall mites, thrips, and total numbers observed were odered gall mites, Diaspididae sp., aphids and thrips. Natural enemies of these hervivores arthropods are such as predatory mites, Chilocorus rubidus, Coccinella septempunctata and the nymph, Aphidius ervi. These results indicate that defense strategies including protective mutualisms may be frequent in the temperate broadleave trees.

• Journal of Korean Society of Forest Science
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• v.104 no.4
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• pp.564-577
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• 2015

The objectives of this study were to investigate the possibility of establishing a tree nursery at Saemangeum reclaimed land and to classify landscape trees based on the salt tolerance. A tree nursery (2.0 ha) was made in Gunsan Okgu area in 2012 with underground drain lines on the reclaimed land established in 2010. Salt content of the nursery soil within the 60 cm depth in 2013 was 5.13 dS/m and 8.20 dS/m for the pre-desalinated and non-desalinated lands, respectively. Thirty-six woody plant species (22 tree species and 14 shrub species at ages of 1 to 4) with a total of 3,943 individuals were planted in early April, 2013 and their growth performance was monitored until September of the same year. The average survival rate of the transplanted plants was 71.4% in late September, suggesting the high possibility of establishing a tree nursery at the reclaimed land. Based on the survival rate and tree vigor (amount of healthy leaves and crown development), the following 17 species with some salt tolerance were classified into three groups: "salt tolerant group" (3 species, Tamarix chinensis, Cudrania tricuspidata, Ilex serrata), "recommended group" (5 species, Pinus thunbergii, Albizia julibrissin, Ligustrum obtusifolium, Rosa rugosa, Pleioblatus pygmaeus), "plantable group" (9 species, Zelkova serrata, Hibiscus syriacus, Elaeagnus umbellata, Sorbus alnifolia, Sophora japonica, Metasequoia glyptostroboides, Quercus acutissima, Ulmus parvifolia, Robinia pseudoacacia). Seven tree species that had been adapted to the reclaimed land for three to four years before being transplanted to new reclaimed land in Gunsan Okgu area showed average survival rate of 98%, suggesting that pre-conditioned trees would survive well in the reclaimed land.