• Fisheries and Aquatic Sciences
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• 제18권4호
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• pp.359-365
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• 2015

We investigated the effects of dietary lipid sources on growth and fatty acid composition of juvenile Chinese longsnout catfish. Triplicate groups of fish (initial average weight, 3.8 g) were fed four diets containing either fish oil (FO), soybean oil (SO), linseed oil (LO) and lauric acid (LA) for 10 weeks. There were no differences among the groups in body weight, feed intake, feed efficiency, protein efficiency ratio, and body proximate composition of fish fed the diets containing different lipid sources (P > 0.05) during the study. However, fatty acids compositions of the whole body were influenced by dietary lipid source. Fish fed the SO diet had high concentration of linoleic acid, whereas those of fish fed the LO diet were rich in linolenic acid and arachidonic acid. Fish fed the FO diet had significantly (P < 0.05) higher levels of monounsaturated fatty acids such as 18:1n-9 and 20:1n-9 than those of fish fed the SO and LO diets. Eicosapentaenoic acid (EPA; 20:5n-3) and docosahexaenoic acid (DHA; 22:6n-3) composition of body were not influenced by dietary lipid source. The results suggest that each of FO, SO, LO or LA can be used as a lipid source in the diets of Chinese longsnout catfish without any negative effects on growth and feed utilization and these data demonstrate the potential impact which dietary fat composition can change the body fatty acid profile.

• Journal of Nutrition and Health
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• 제25권5호
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• pp.339-350
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• 1992

This study was performed to investigate the effect on polyunsaturate fatty acid diets and feeding periods on the antithrombosis. the hematological changes in the blood and fatty acid compositions of platelets in rats. Each group of rats was fed a diet containing 20%(W/W) corn oil beef tallow sardine oil and the general stock diet for 10, 20. 40 and 80 days. Rats fed sardine oil diet showed significantly longer bleeding time than any other diet groups after 20 days feeding The whole blood clotting time of sardine oil group fed for 80 days was increased significantly. The number of platelet and the concentration of hemoglobin showed no significant difference among all groups. The number of white blood cell was decreased continously in sardine oil group after 10 days feeding. The level of malondialdehyde generation during thrombin-induced aggregation of platelets was decreased continously in sardine oil grou after 20 days feeding. With regard to the composition of platelet fatty acid the ratio of eicosapentaenoic acid(EPA 20: 5 $\omega$-3) to arachidonic acid(AA 20:4 $\omega$-6) was increased in sardine oil group but decreased in corn oil groups and beef tallow groups with days. In conclusion the rats fed sardine oil diet for more than 20 days showed the fact that EPA induced the antithrombosis. the changes in number of white blood cell and the fatty acid composition of platelets.

• Journal of Nutrition and Health
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• 제26권5호
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• pp.513-523
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• 1993

In this experiment, we investigated the hypolipidemic and antithrombotic effects of rats fed diets with different common oils in Korea for different feeding periods(4 weeks or 12 weeks), using Korean sesame oil, perilla oil, rice bran oil and mixed oil. W-3/w-6 ratio of each group was 0.001, 1.44, 0.03 and 0.112, respectively. P/S ratio of each group was 9.64, 10.49, 5.58 and 1.68, respectively. The result were as follows: 1) According to the age, body fat accumulation was increased. 2) Perilla oil(w-3 rich) decreased total lipid, triglyceride and total cholesterol, and increased HDL/total cholesterol ratio. 3) With regard to the compositono of platelet fatty acids, Perilla oil increased w-3/w-6 ratio of the platelet. Perilla oil lengthened bleeding time and decreased MDA(MalonDAdehyde) formation which determined in place of Thromboxane A2(TXA2) in platelet. This result can suggest that linoleic acid of perrilla oil seem to supress the conversion of linoleic acid to arachidonic acid(AA 20:4, w-6) and eicosapentaenoic acid(EPA, 20:5, w-3) trannnsformed from linolenic acid to suppress the conversion of arachidonic acid to TXA2. Since TXA2 is platelet-aggregating and vasoconstricting agent, the reduction of TXA2 tgeneration by platelet with increased linolenic acid intakes shows prologed bleeding time. In conclusion, w-3 rich perilla oil has strong hypolipidemic and antithrombotic effects by changing fatty acid profiles of the platelet.

• Asian-Australasian Journal of Animal Sciences
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• 제30권7호
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• pp.973-978
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• 2017

Objective: The aim of this study was to investigate the effects of simultaneous supplementation of laying hens with alpha-linolenic acid (ALA) resources (flax, perilla, and Eucommia ulmoides [E. ulmoides] seeds) and eicosapentaenoic acid/docosahexaenoic acid (EPA/DHA) resources (Schizochytrium sp.) on egg quality and n-3 polyunsaturated fatty acids (PUFA) profile. Methods: Dietary treatments were as follows: i) diet C (control diet); ii) diet F (diet C+10% flaxseeds); iii) diet P, (diet C+10% perilla seeds); iv) diet E (diet C+10% E. ulmoides seeds); v) diet A (diet C+1.5% microalage); vi) diet AF (diet C+10% flaxseeds+1.5% microalage); vii) diet AP (diet C+10% perilla seeds+1.5% microalgae); viii) diet AE (diet C+10% E. ulmoides seeds+ 1.5% microalage). Results: Egg weight, yolk weight and production ratio were not significantly affected by either algae or in combination with seeds (p>0.05). No significant difference was observed in ALA and DHA concentration in eggs between flaxseed, perila, and E. ulmodies seeds supplementation alone (p>0.05). N-3 PUFA in eggs was slightly improved by microalgae supplementation. The best supplementation, a combination of microalgae and perilla seeds, elevated (p<0.05) ALA from 19.7 to 202.5 mg/egg and EPA+DHA from 27.5 to 159.7 mg/egg. Highest n-3 PUFA enrichment (379.6 mg/yolk) was observed with supplementation of a combination of perilla seed and microalgae (362.2 mg/yolk), followed by a combination of flaxseed and microalgae (348.4 mg/yolk). The ALA, EPA, and DHA content obtained with a combination of microalgae and seeds surpassed the total sum of that obtained with microalgae or ALA-seeds alone. Conclusion: It is feasible to enrich eggs with n-3 PUFAs by perilla or E. ulmodies seeds instead of flaxseeds. Simultaneous supplementation of microalgae and seeds helped improve the transfer from EPA and docosapentaenoic acid into DHA.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1993년도 제2회 신약개발 연구발표회 초록집
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• pp.83-83
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• 1993

생체내 기능조절에 중요한 역할을 하는 prostaglandins(PGs)는 신경계 세포, 순환기계세포, 거삭세포, 섬유아세포 및 암세포 등 여러 부위에 널리 분포되어 있다. 본 연구에서는 PGs생체내 전구체인 arachidonic acid와 구조유사체인 eicosapentaenoic acid(EPA) 및 docosahexaenoic acid(DHA)에 의한 순환기계효과를 TXA$_2$ 생합성과 관련하여 규명하고져 하였다. 또한 여러 가지 면역요법 중 암세포 살해능이 있는 lymphokine activated killer(LAK) 세포의 생성력과 생체내 면역세포 중 암세포의 일차적인 방어작용을 하는 것으로 알려진 natural killer(NK) 세포의 활성도를 PGs생성과 연관시켜 검토하므로써 암환자에 저하되어 있을 면역기구와 PGs와의 상관관계를 규명하고자 하였다.

• 한국식품저장유통학회:학술대회논문집
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• 한국식품저장유통학회 2003년도 제23차 추계총회 및 국제학술심포지움
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• pp.141.2-141
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• 2003

고도불포화지방산 중 EPA(eicosapentaenoic acid)와 DHA(docosahexaenoic acid)는 여러 가지 생리활성 효과를 나타내는 것으로 알려져 있으며, 최근 관심이 주목되고 있다. 본 실험에서는 특히 DHA의 함량이 높은 해양미생물, 즉 조류(microalgae)인 Schizochytrium sp.에서 얻어진 DHA powder로부터 두 가지 방법으로 유지를 추출한 뒤 그 성질을 비교.분석하였다. 먼저, 8$0^{\circ}C$, soxhlet extractor로부터 6시간 동안 추출하고, 55$^{\circ}C$, shaking water bath로부터 같은 시간 동안 추출한 결과, 각각 25.5, 24.0%의 수율을 나타내었다. GC에 의한 지방산 조성 분석 결과, soxhlet extractor에서 추출한 경우, palmitic acid, DHA가 각각 30.98, 30.04 mol%로 주요 지방산인 것으로 나타났으며, shaking water bath에서 추출된 유지는 그 함량이 각각 31.44, 34.78 mol%를 나타내었다. 또한 pancreatic lipase에 의한 가수분해를 통해 sn-2 위치의 지방산 조성 분석 결과, DHA가 sn-2 위치에 주로 함유되어있는 것으로 조사되었다. 한편, HPLC-ELSD system을 이용하여 중성지방의 구성 성분을 분석 결과, 유지의 99%이상이 TAG(triacylglycerol) 형태로 이루어져 있었으며, 소량의 MAG(monoacylglycerol) 존재를 확인할 수 있었다.

• Journal of Medicine and Life Science
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• 제18권1호
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• pp.1-10
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• 2021

Omega-3 fatty acids (eicosapentaenoic acid [EPA] and docosahexaenoic acid [DHA]) may be beneficial for the primary and secondary prevention of cardiovascular events (CVEs), especially in patients with myocardial infarction or heart failure with reduced ejection fraction. For this purpose, one to two seafood meals per week is preferentially recommended. Omega-3 fatty acids with a high-dose EPA formula (4 g/day) may be more effective than EPA+DHA mixed supplements for the secondary prevention of CVE. Krill oil also contains omega-3 fatty acids, but at a much lower dose compared to fish oil. Supplemental vitamins and minerals have not shown the preventive effects on CVE in prospective, and randomized clinical trials, except for one Chinese study showing the stroke prevention effects of folic acid. The clinical benefit of chelation therapy in reducing CVEs is uncertain.

• Journal of Microbiology and Biotechnology
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• 제23권10호
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• pp.1413-1421
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• 2013

The marine microalga Isochrysis sphaerica is rich in the very-long-chain polyunsaturated fatty acids, including eicosapentaenoic acid (EPA, $C20:5{\omega}-3$) and docosahexaenoic acid (DHA, $C22:6{\omega}-3$) that are important to human health. Here, we report a functional characterization of a ${\Delta}4$-fatty acid desaturase gene (FAD4) from I. sphaerica. IsFAD4 contains a 1,284 bp open reading frame encoding a 427 amino acid polypeptide. The deduced amino sequence comprises three conserved histidine motifs and a cytochrome b5 domain at its N-terminus. Phylogenetic analysis indicated that IsFad4 formed a unique Isochrysis clade distinct from the counterparts of other eukaryotes. Heterologous expression of IsFAD4 in Pichia pastoris showed that IsFad4 was able to desaturate docosapentaenoic acid (DPA) to form DHA, and the rate of converting DPA to DHA was 79.8%. These results throw light on the potential industrial production of specific polyunsaturated fatty acids through IsFAD4 transgenic yeast or oil crops.

• Journal of Nutrition and Health
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• 제27권9호
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• pp.901-909
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• 1994

The effects of sardine oil(high in eicosapentaenoic acid : EPA) and tuna oil(high in docosahexaenoic acid : DHA, also high in EPA) on fatty acid composition of brain and learning ability were evaluated in male weanling Sprague-Dawley rats and compared with the effects of corn oil and beef tallow. Animals assigned by randomized block design to one of the four experimental diet groups containing dietary lipid at 15%(w/w) level were given ad libitum for 7 weeks. Food intake and body weight gain of the fish oil groups were significantly lower than those of the corn oil and beef tallow groups. However, brain weights of the groups were not significantly different. In the brain fatty acid composition, the corn oil group showed high concentrations of n-6 fatty acids, the fish oil groups of n-3 fatty acids, and the beef tallow group of saturated fatty acids. Brain EPA and DHA contents of the fish oil groups showed significantly higher than the other groups while the brain ratio of saturated/monounsaturated/polyunsaturated fatty acid was controlled in a narrow range. In a maze test, the fish oil groups appeared to arrive at the goal faster than the corn oil and beef tallow groups. It explained that EPA in diets might efficiently convert to DHA resulting in DHA accumulation in brain tissue and might increase the learning performance as DHA did.

• Ocean and Polar Research
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• 제38권1호
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• pp.35-46
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• 2016

To understand the diet of chaetognaths, the gut content and fatty acid trophic makers (FATMs) of Sagitta crassa and S. nagae, which are the most predominant species of chaetognath in the Yellow Sea, were analyzed. Gut contents of the two species examined by microscopic analysis revealed that copepods are the major components of the diet (> 70% of gut contents) and there was no significant changes in the gut contents of two species collected in spring and summer season. Although 16:0, 20:5(n-3) (Eicosapentaenoic acid) and 22:6(n-3) (Docosahexanoic acid), which are known as phytoplankton FA markers, were the most dominant among the fatty acids in both chaetognath species, the detection of copepod FA markers, 20:1(n-9) (Gadoleic acid) and 22:1(n-11) (Cetoleic acid), provided evidence that their food sources include copepods. These results suggest that S. crassa and S. nagae are carnivores and mainly feed on copepods in the Yellow Sea.