• Journal of Nutrition and Health
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• v.18 no.4
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• pp.301-311
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• 1985

Even though the anticarcinogenic effect of dietary factors especially beta - carotene has been reported by various investigators, the mechanism of the action of ${\beta}-carotene$ has not yet been identified. We carried out the present study to determine the possibilities of relative cancer risk related to dietary intake of vitamin A ( both ${\beta}-carotene$ and retinol ) and blood levels of vitamin A among Koreans. The subjects were divided into two groups; cancer patients and controls. Blood levels for ${\beta}-carotene$ and retinol were analyzed by alumina column chromatography and colorimetry. Dietary intake was examined by food profile and convenient method for evaluating nutritional status through recalling 10 years of food habits. The results obtained are as follows : 1 ) Calorie, protein, fat, and carbohydrate intakes of cancer patient were lower than those of control. Calorie and carbohydrate intakes showed no significant difference but protein and fat intakes were significantly lower in cancer patients. According to cancer sites, in stomach cancer only fat intake was significantly lower than that of control. In lung and larynx cancer calorie, protein, fat and carbohydrate intakes showed similar trend as in control. 2 ) Vitamin A intake of cancer patient was significantly lower than that of control. It was estimated that 83.6% of total Vitamin A intake were provided by ${\beta}-carotene$ for control and cancer patient respectively. 3 ) The mean intake of dietary ${\beta}-carotene$ in cancer patient was significantly lower than that in control ( $7002\;\mu}g/day$ versus $10326\;{\mu}g/day$ ) According to cancer sites in mean intake of dietary ${\beta}-carotene$ was significantly lower in all but stomach cancer compared with that of control. Lung and larynx cancer showed lowest ${\beta}-carotene$ intake with mean value of $5855{\mu}g/day$ and $5492{\mu}g/day$ respectively. 4 ) The mean intake of dietary retinol in cancer patient was significantly lower than that in control ( $245{\mu}g/day$ versus $338{\mu}g/day$ ), but the difference was not significant. 5 ) The relative risk of all cancers in the first (lowest) to the forth quartile level of ${\beta}-carotene$ consumption such as $0-5999{\mu}g/day$. $6000-8999{\mu}g/day$, $9000-11999{\mu}g/day$/ day and $12000-20000{\mu}g/day$ was 85 : 1.7 : 20 : 1.0. The relative risk of all cancers in the first (lowest) to the forth quartile level of retinol consumption, such as $0-299{\mu}g/day$, $300-599{\mu}g/day$, 600-899${\mu}g/day$, and $900-1200{\mu}g/day$, was 1.14 : 067 : 0.21 : 1.0. 6 ) The various food group consumption of cancer patient were significantly lower than those of control in green leafy vegetables, fruits, sea weeds, milk and cheese and eggs. But the Kimchie consumption in cancer patient was three fold higher than that of control ( $1840\;{\mu}g/day$ versus $562\;{\mu}g/day$ ) and in the stomach cancer, Kimchie consumption was the highest, ( $1890\;{\mu}g/day$) According to cancer sites, the consumption of green leafy vegetables was significantly lower in all but stomach cancer compared to control and other vegetables showed no difference between two. In lung and larynx cancer, green leafy vegetables consumption was lowest ( $6094{\mu}g/day$ $5921{\mu}g/day$) and milk and cheese consumption was also( $5\;{\mu}g/day$ and $11{\mu}g/day$) 7 ) The recovery of ${\beta}-carotene$ from human serum by alumina column chromatography was $94.4{\pm}2.3%$. 8 ) Cancer patients showed significantly lower serum retinol ($56.4{\pm}18.1\:{\mu}g/100ml$ versus $72.2{\pm}21.8\:{\mu}g/100ml$) and ${\beta}-carotene$ ($48.9{\pm}33.8\:{\mu}g/100ml$ versus $72.2{\pm}42.6\:{\mu}g/100ml$) concentrations than in controls. 9 ) But breast cancer patients were not significantly different from controls in their serum retinol and ${\beta}-carotene$ concentrations.

• Korean Journal of Ichthyology
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• v.1 no.1_2
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• pp.73-82
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• 1989

The development of eggs and larvae, and minute scale-like tubercles on the skin surface of the larval Acanthorthodeus asmussi from Korea were observed in the laboratory. The egg was nearly ovoid-shaped. The number of egg averaged 195 per an oviposition. The morphological character of larval development was relatively similar to those of Acheilognathus tabira tabira, A. tabira subsp. (a), A. yamatsutae, A. moriokae, A. cyanostigma and A. rhombeus. The larvae of this species had many scale-like tubercles ellipsoided in a diagonal cross section on the skin surface of the body. And also this species moved like incessant wiggly movement pattern as that of fly maggot duing the larval development. As regards the tubercles and larval movement pattern, the larvae of A. asmussi shared similar characters with those of Acheilognathus rhombeus, A. longipinnis and Pseudoperilampus typos. Hence A. asmussi may be closely related to these species. The latter three species are autumn-spawning bitterlings and the larval development always retard in a certain stage, while A. asmussi is a spring-summer spawning species and the larval development never retards. Judging from these facts, it should be considered that there are close relations in evolutionary trend between the two spawning species.

• Journal of Embryo Transfer
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• v.17 no.1
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• pp.45-53
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• 2002

The main goal of this study was to produce transgenic porcine embryos by direct injection of sperm-mediated exogenous DNA. Spermatozoa (6$\times$10$^{6}$ sperms of final concentration) were mixed with pcDNA LAC Z (20 ng/$\mu$l) and subjected into electroporation (300~750 volts, 25 $\mu$F, 0.4 cm electrode). After sperm injection, the oocytes were activated electrically (1.7 KV/cm, 30$\mu$sec, single pulse) in 0.3 M mannitol solution or not. The sperm injected eggs were cultured in NCSU 23 medium (0.4% BSA) at 39$^{\circ}C$, 5% $CO_2$ in air fur 144 h. The rates of cleavage and development into blastocyst stage in activation group were significantly higher than those of non-activation group (79.6% and 24.1% vs. 46.3% and 14.4%, respectively, p<0.05). Control oocytes and shame injection were developed to blastocysts low (2.5%). Sixty five (27.1%) out of 240 embryos observed in activation and non-activation groups were showed positive by X-gal staining. However, all embryos in both groups were expressed partial or mosaic pattern. These results suggested that electrical stimulation far oocytes activation after sperm injection enhances the incidence of both fertilization and development fellowing sperm injection in the pig. Our study also suggested that sperm-mediated transfer of exogenous DNA by ICSI would be used as a valuable tool for the production of transgenic porcine embryos.

• Culinary science and hospitality research
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• v.17 no.3
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• pp.204-220
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• 2011

of the recipes and 'stir frying' in 76.8% of the recipes. Other cooking methods were used as follows; 'blanching'(24.2%), 'roasting'(22.6%), 'pan frying'(21.0%), 'deep fat frying'(18.7%), etc. The use of 'Boiling' increased significantly(p=0.044) from 4.5% in 2008 to 10.0% in 2010 while the use of 'deep fat frying' decreased significantly(p=0.027) from 21.8% to 10.9%. The frequency of using cooking tools was as follows; knives and cutting boards(100.0%), dishes(92.9%), frypans(91.3%), chopsticks(40.3%), spoons(38.7%), etc. The foods used in cooking were as follows; rice(100.0%), carrots(67.1%), onions(61.9%), eggs(41.6%), paprika(27.7%), kimchi(25.2%), pizza-cheese(22.9%), etc. Natural and processed foods were used in cooking as the ratio of 80:20 approximately. Of the 5 food groups, 'meat fish egg bean' decreased from $24.44{\pm}13.27%$ in 2008 to $20.84{\pm}10.59%$ in 2010, but 'vegetables' increased from $44.38{\pm}15.65%$ to $50.64{\pm}14.07%$ significantly(p<0.05). In conclusion, 5th and 6th graders had an ability to select various foods for their health when cooking even if they have some foods they don't like while they lacked cooking skills such as using various cutting methods and proper cooking tools.

• Korean Journal of Poultry Science
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• v.38 no.3
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• pp.205-211
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• 2011

This work was carried out to investigate the effect of the induced molting diet based on wheat bran on the postmolt performance of layers. Two hundred White Leghorn layers (65-old-wk) with over 80% egg production were used for 8 weeks in this work. Treatments were non-molt control (CO), fasting treatment for 10 days (FW), molt treatment with used molting diet for 4 wk (UM), molt treatment with molting diet based on corn-wheat bran for 4 wk (CW), and molt treatment with molting diet based on wheat bran for 4 wk (WM) as 5 treatments (4 replications/treatment and 10 birds/replication). Feed intake decreased at molting treatments at first weeks and increased after the 3rd week compared to control (P<0.05). Body weight (BW) loss were 18.6% of initial BW at first week in FW treatment, and were 11.4, 14.2 and 17.4% in UM, CW and WM treatments at 4th weeks (P<0.05). Egg production decreased at 1 week in molt treatment and stopped at 2 week in FW, whereas, other molting treatments didn't stop laying eggs. The birds started to lay egg at 4 week in FW and recovered at 5 week in other molting treatments. Egg quality (eggshell thickness, eggshell breaking strength, haugh unit) was high in molting treatments compared with control at 8th weeks (P<0.05). Finally, molting diet based on wheat bran affected BW loss and egg quality such as eggshell thickness, eggshell breaking strength and haugh unit.

• Korean Journal of Poultry Science
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• v.31 no.4
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• pp.265-271
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• 2004

A feeding trial was conducted to investigate the influence of feeding Lactobacillus reuteri culture (LR) on productive performance, intestinal microflora and availability in laying hens. Four hundred and eighty, Isa-Brown layers, 49 weeks of age, were fed diets supplemented with LR at the level of 0 (control), 0.1, 0.2, and $0.4\%$ of the diets for eight weeks. Egg production and egg weight were measured daily. Feed intake was weighed every two weeks. Egg quality was measured three times at the start, mid-term, and end of the experiment. Intestinal microflora were examined for Lactobacillus spp., E. coli and Salmonella at the end of the experiment. Overall egg production was the highest in $0.2\%$ LR (P<0.05), but that of $0.1\%$ or $0.4\%$ LR treatments did not significantly differ from that of control. Egg weight was significantly higher in LR feeding group than the control (P<0.05). Daily egg mass was significantly higher in $0.2\%$ and $0.4\%$ LR treatments compared to the control and $0.1\%$ LR (P<0.05). The number of jumbo and extra large eggs were increased in LR supplemented groups, especially in $0.1\%$ LR. Feed intake of layers fed LR supplemented diets tended to be lower than the control. However, feed conversion ratio significantly improved in LR supplemented groups (P<0.05). Availability of dry matter and crude protein improved significantly in $0.4\%$ LR treatment (P<0.05). But, those of ether extract and crude ash were not significantly different among treatments. Eggshell breaking strength and eggshell thickness were not significantly influenced by LR supplementation, and Haugh unit and yolk index were also similar to the control. Total number of Lactobacillus spp. in ileum and cecum fed LR supplemented diets were significantly higher than those of the control (P<0.05). There were no significant differences in intestinal E. coli and Salmonella in all treatments. Therefore, it is concluded that dietary supplementation of Lactobacillus reuteri culture can improve the laying performance, feed efficiency and intestinal Lactobacillus.

• Korean Journal of Poultry Science
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• v.31 no.4
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• pp.299-306
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• 2004

The objective of this study was to investigate the influence of egg storage, broiler breeder age, and the change of egg weight during incubation on growth rate of chicks and 43-day-old dressing percentage. The trials involved hatching eggs obtained from 27-wk-old hens and stored for 6 d for the Young-EXP group, from 28-wk-old hens and stored for 0 d for the Young-CON group, from 51-wk-old hens and stored for 6 d for the Old-EXP group, and from 52-wk-old hens and stored for 0 d for the Old-CON group, The hens were two commercial broiler breeder flocks of the same strain (Cobb) but of different egg producing stages(early and middle stages of egg production). The chicks were grown on floor pens for 6 wks, The differences of setting egg weights between Old-CON and Old-EXP groups were 1 g, but those between Young-CON and Young-EXP groups were 2.9 g(P<0,05). The loss of egg weight during 18 d incubation did not greatly differ among four groups, but the loss of egg weight during 21 d incubation was significantly (P<0.05) more in the middle stage of egg production groups than in the early stage of egg production groups. The mean birth weights of the middle stage of egg production groups were significantly(P<0,05) heavier by 8,7 g than those of the early stage of egg production groups; however, the differences of 6-wk-old body weight were not significant between egg producing stages. The differences of body weights in both egg producing stages were not significantly influenced by egg storage period in overall wks of ages. Egg storage and hen age did not greatly influence to the 43 d dressing percentages, either, The correlations of the setting egg weight with 18 d egg weight during incubation, growth rate of chicks, or 43 d dressing percentage were not significant.

• Korean Journal of Poultry Science
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• v.31 no.4
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• pp.283-291
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• 2004

Feeding trials were conducted with Euglena strains grown under different media. The effect of supplementation of Euglena on the laying performance, egg quality and fatty acid composition of egg yolk was studied. In experiment I, two hundred eighty 32-wk-old ISA Brown layers were randomly assigned to seven dietary treatments for 4 wks. Each treatment consisted of 4 replications with 10 birds each housed in two birds cages. Control diet was formulated to have $17\%$ CP and 2,750 kcal ME/kg. Euglena gracilis Z. (EG) was added to control diet at the level of 0.25, 0.5, $1.0\%$ and Euglena gracilis Z. bleached and DHA enriched (EGBD; a strain mutated by streptomycin and cultivated in DHA enriched medium) at the level of 0.5, 1.0, $2.0\%$ in the diet. In experiment 2, three hundred 84-wk-old ISA brown layers were randomly assigned to five dietary treatments: T1; Control, T2; T1 + EGBD $0.5\%$, T3; T1 + Euglena gracilis Z. DHA enriched (EGD; cultivated in DHA enriched medium) $0.5\%$, T4; T1 + EGD $1.0\%$, T5; T1 + EGD $2.0\%$. Each treatment had 5 replication of 12 birds each housed in two birds cages. In experiments 1 and 2, Euglena suppplementation did not significantly affect egg production but increased egg weight and feed intake. In experiment 1, EG was more effective in increasing egg yolk color score than EGBD. Egg yolk color of EG $1\%$ treatment showed the highest score. EGBD supplementation increased DHA concentration of egg yolk. EGBD $2\%$ treatment showed the highest DHA and the lowest palmitic and stearic acids concentration in the egg yolk. In experiment 2, EGBD $0.5\%$ treatment showed highest DHA level in egg yolk (P<0.05). It was conducted that EGBD is a single cell protein source rich in DHA, that can be used to produce DHA enriched eggs.

• Korean Journal of Poultry Science
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• v.16 no.4
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• pp.193-199
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• 1989

This study was conducted to estimate heritabilities and genetic correlations on egg compositions in Korean Native Ogol Fowl. The date analysis were a total of 58,320 eggs in 450 pullets producted from 150 dams and 20 sires of Korean Native Ogol Fowl raised at Chungnam National University from June 18. 1987 to April 6. 1989. The results obtained are summarized as follows： 1. On the egg compositions, the albumen weight at first egg, 300 and 500 days were $24.003\pm$2.499, $28.354\pm$2.755 and $31.863\pm$3.341g； the yolk weight were $10.272\pm$1.536, $16.346\pm$1.321 and $19.212\pm$1.661g： the shell weight were $3.906\pm$0.414, $4.336\pm$0.506 and $4.822\pm$0.515g, respectively. 2. The heritability estimates of egg compositions based on the valiance of sires, dams and combined components were 0.620-0.723, 0.206-0.300 and 0.413-0.511 for albumin weight；0.439-0.737, 0.484-0.544 and 0.492-0.615 for yolk weight：0.172-0.187, 0.412-0.642 and 0.390-0.503 for shell weight, respectively. 1. The genetic and phenotypic correlation coefficients of egg compositions were as follows； The coefficients between albumen weight and yolk weight were 0.089-0.654 and 0.084-0.255；between albumen weight and shell weight were 0.396-0.925 and 0.225-0.544； between yolk weight and shell weight were 0.227-0.357 and 0.098-0.358 respectively.

• Korean Journal of Poultry Science
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• v.18 no.3
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• pp.183-196
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• 1991

In order to establish ELISA method to detect antibody against IBV various factors involved were examined. Antigen was prepared from Massachusetts type IBV which is known to be one of serotypes distributed most widely. The virus was grown in embryonated SPF chicken eggs. Allantoic fluid harvested was processed to ultracentrifugation and sucrose density gradient centrifugation to produce a purified antigen The antisera selected from the field samples based on hemagglutination inhibition test were used as the standard positive and negative sera for this study and the results obtained were summarized as follows. 1 , It was found that ELISA test was satisfactory when the purified antigen was coated on the plate in the amount of about 40ng protein per well. In case of the phospholipase treated hemagglutinating antigen it gave satisfactory results when the each well wns coated with 1.2 to 2.5 hemagglutinating unit which was equivalent to 40 to 90ng of protein. 2. There was no significant difference in the ratio of optical density of positive to that of negative serum whether the coated antigen was held for 1 hour at 37$^{\circ}C$ or it was held overnight at 4$^{\circ}C$. The coated antigen could be kept in dried state without change of antigenecity for at least one month of experimental period at 4$^{\circ}C$. 3. There was a big variation in the optical density and P/N values depending on the maker of the plates and on the plate of the same maker. 4. It was found that background optical density was negligible when serum was diluted more than 1：50 and serum dilution of 1：100 appeared to be appropriate as a routine test dilution to screen the antibody. 5. Optical density was fairly constant 15 minutes afterward from the time substrate was treated and during the 4 hours after stopper was treated. 6. There was a low correlation(r＝0.42) between ELISA and HI test. However, when 74serum samples were tested for the IBV antibody, 98.7% were found to be positive by both tests in which titers of 2$^{6}$ or more by HI test and P/N values of 1.4 or more by ELISA were considered to be positive, 7 Day-old IBV vaccinated chickens shows a similar antibody decay and rising pattern until 8 weeks of age by the two tests, ELISA and HI.