• The Korean Journal of Ecology
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• v.1 no.2
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• pp.44-48
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• 1977

The spawning behavior and developmental pattern of the Korean eleotrid fish (Odontobutis obscurus) was investigated in one of the tributary of Han River. The spawining of this species begins on April and continues up to August, whereas, the spawning. of same species were between the end of June and the middle of July in Kyushu, Japan. August The spawning places were the margin of river, its the bottom composed of sand and pebble (Table 5). The velocity of water in the spawning places was 0-40cm/sec, and the depth of waters was 10-40cm. The egg was spawned on underside of stone in the spawning places with one or two folds and ellipsoid in shape, 3.4mm in long diameter and 2.0mm in short diameter. The yolk is 1.2mm in diameter and yellow in color. For hatching of the egg, it takes one month in, $16^{circ}C~19^{\circ}C$ water, in Japan, whereas it takes only 14 days in $20^{circ}C~30^{\circ}C$ wate rin the study area. The growth rate of larva and juvenile fish were as follows: new hatched larva is 45mm in length; after 5 days it became 5.0 to 5.5mm in length and 1.2 to 1.5mm in height after 20 days. it became 11.0mm in length and 2.3mm in length. The mean size of the mature female is 110mm in total length and larger 10mm than that of the same fish found in Japan.

• Journal of Animal Science and Technology
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• v.63 no.6
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• pp.1275-1285
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• 2021

The objective of the present experiment was to investigate the effect of dietary palm kernel meal (PKM) and β-xylanase supplementation on productive performance, egg quality, fatty liver incidence, and excreta characteristics in laying hens. A total of 320 Hy-Line Brown laying hens (33 weeks of age) were allotted to 1 of 4 treatments with 8 replicates in a feeding trial. Each replicate consisted of 10 consecutive cages with 1 hen per cage. The corn-soybean meal-based control diet was prepared. Additional diet was prepared by including 10% of PKM in the control diet with a partial replacement of corn, soybean meal, and animal fat. In addition, 0.025% β-xylanase was supplemented at the expense of celite to those 2 diets to produce 4 treatment diets in a 2 × 2 factorial arrangement. All hens were provided the diet and water ad libitum for 8 weeks. Results indicated no significant interactions between inclusion of dietary PKM and β-xylanase for all measurements; therefore, the main effects were mainly discussed. Hens fed diets containing 10% PKM had greater (p < 0.05) feed intake and yolk color than those fed diets containing no PKM. However, dietary PKM did not influence fatty liver incidence and excreta characteristics. Dietary β-xylanase supplementation had no effects on all measurements, regardless of inclusion of PKM. In conclusion, PKM can be a potential feed ingredient for laying hens at the inclusion of 10% in the diet. It appears that dietary β-xylanase used in the current experiment has little effect on layer productivity, regardless of inclusion of 10% PKM in the diet.

• Proceedings of the Korea Society of Poultry Science Conference
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• 2004.11a
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• pp.35-36
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• 2004

A total of 1.200 eggs obtained from 312-day-old Hy-line Brown layer breeder hens and 319-day-old Hy-line Brown commercial layer hens (600 eggs obtained from each ones) were used to investigate the effects of storage period, storage temperature, and insemination of hens on the change of albumen height, Haugh unit (HU), albumen pH, shell strength, and yolk color. Eggs were stored up to 14 days after lay at $3\;^{\circ}C\;or\;10\;^{\circ}C$ and sampled one day after stored and then 24 hours interval. Longer periods of storage resulted in lower albumen height and HU at both storage temperatures, but in higher albumen pH. The eggs stored at $3\;^{\circ}C$ were generally higher in HU and lower in albumen pH than the ones stored at $10\;^{\circ}C$. There was no statistically difference although the eggs obtained from the non-inseminated-hens were slightly higher in albumen height and HU than the eggs obtained from the inseminated-hens. Whereas, the eggs obtained from the non-inseminated-hens in the albumen pH of eggs stored at $3\;^{\circ}C$ was significantly (P<0.05) higher than the ones obtained from the inseminated-hens, but the albumen pH of eggs stored at $10\;^{\circ}C$ did not differ each other. The mean shell strength of the eggs obtained from the inseminated-hens was significantly (P<0.05) stronger than that of the eggs obtained from the non-inseminated-hens at both storage temperatures. Albumen height and albumen pH were negatively correlated(P<0.01~0.001) in both inseminated and non-inseminated-hen's egg groups. The degree of yolk colors were not significantly changed overall of the experimental periods in both storage temperatures. The study suggests that the change of egg freshness such as albumen height and HU are relatively more associated with storage period and storage temperature than insemination or non-insemination of hens.

• Korean Journal of Ichthyology
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• v.10 no.2
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• pp.184-190
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• 1998

The early life history of black bullhead, Pseudobagrus koreanus, endemic to Korea was investigated to get biological information needed in artificial production of seedlings and in recovering natural resources. The fertilized eggs showed some characteristics in having heavy sticky material and minute folds which is formed radical pattern on the egg membrane. The shape of egg was spherical and $2.59{\pm}0.08$(2.45~2.70, n=10)mm in diameter. The yolk had not oil globule. The first cleavage was observed 2 hrs after insemination at $21{\sim}23^{\circ}C$, and the progressive cleavage were done about 30 min. interval. The characteristic changing of the yolk surface started at morula stage and continued to the end of gastrula. Hatching was started 72 hrs and completed 90 hrs after fertilization. The size of the larvae were 5.41~5.81mm in total length and 2.76~2.94mm in preanal length, and the number of so mites was 15-16+33~34(48~50). The barbels and swimbladder were completed and all the fins except second dorsal were appeared 1 week after hatching. The larvae attained 9.67~10.52mm in total length and 5.20~5.65mm in preanal length. All the fin sets and color pattern were completed 2 weeks after hatching and body mucus was secreted at that stage. The juvenile attained 14.59~16.02mm in standard length, 3.31~4.16mm in head length and 8.07~9.31mm in prenal length.

• Korean Journal of Poultry Science
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• v.49 no.4
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• pp.247-253
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• 2022

This study was conducted to investigate the effects of γ-aminobutyric acid (GABA) and a probiotic mixture on egg quality, blood parameters, and corticosterone content in broiler breeder stock in summer in Korea. A total of 72 broiler breeders (Arbor Acres) at 49 weeks old were randomly divided into the following three groups of 24 heads each: control, GABA, and probiotics (1 × 10⁸/g Bacillus licheniformis, 1 × 10⁷/g Lactiplantibacillus plantaru, and 1 × 10⁷/g Clostridium butyricum). The hens were fed a diet containing 50 mg of GABA/kg of feed and 0.1% probiotics for 6 weeks. Egg quality, blood parameters, and stress levels (corticosterone concentration) of the broiler breeders were evaluated. No significant differences were observed in the egg weight, albumen height, Haugh units, yolk color, shell thickness, or shell strength between the groups. There were no significant between-group differences in the blood biochemical profile, including total cholesterol, triglyceride, glucose, total protein, aspartate aminotransferase, alanine aminotransferase, albumin, and inorganic phosphorus concentrations. The corticosterone concentration was significantly lower in the GABA and probiotics groups than the control group (P < 0.05) at 6 weeks of age. In conclusion, these results suggest that dietary probiotics and GABA significantly improve the stress levels of broiler breeders in summer in Korea.

• Animal Bioscience
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• v.37 no.1
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• pp.95-104
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• 2024

Objective: In the present study, we aimed to investigate the effects of enzymolysis fermentation of Chinese herbal medicines (CHMs) on egg production performance, egg quality, lipid metabolism, serum reproductive hormone levels, and the mRNA expression of the ovarian hormone receptor of laying hens in the late-laying stage. Methods: A total of 360 Hy-Line Brown laying hens (age, 390 days) were randomly categorized into four groups. Hens in the control (C) group were fed a basic diet devoid of CHMs, the crushed CHM (CT), fermented CHM (FC), and enzymatically fermented CHM (EFT) groups received diets containing 2% crushed CHM, 2% fermented CHM, and 2% enzymatically fermented CHM, respectively. Results: Compared with crushed CHM, the acid detergent fiber, total flavonoids, and total saponins contents of fermented CHM showed improvement (p<0.05); furthermore, the neutral and acid detergent fiber, total flavonoids, and total saponins contents of enzymatically fermented CHM improved (p<0.05). At 5 to 8 weeks, hens in the FC and EFT groups showed increased laying rates, haugh unit, albumin height, yolk color, shell thickness, and shell strength compared with those in the C group (p<0.05). Compared with the FC group, the laying rate, albumin height, and Shell thickness in the EFT group was increased (p<0.05). Compared with the C, CT, and FC groups, the EFT group showed reduced serum total cholesterol and increased serum luteinizing hormone levels and mRNA expressions of follicle stimulating hormone receptor and luteinizing hormone receptor (p<0.05). Conclusion: These results indicated that the ETF group improved the laying rate and egg quality and regulated the lipid metabolism in aged hens. The mechanism underlying this effect was likely related to cell wall degradation of CHM and increased serum levels of luteinizing hormone and mRNA expression of the ovarian hormone receptor.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.43 no.1
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• pp.46-53
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• 2010

Spawning behavior and early life history of the tuman river sculpin, Cottus hangiongensis were studied in the laboratory and in the field at Wangpi Stream, Gyeongsangbuk-do, Korea, from January to December, 2007. The spawning ground was in the lower Wangpi Stream, which is a shallow region about 40cm or less in depth. During the spawning period, from March to April, mature males made nest cavities under stone 10 which they led a gravid female. The male and female then turned upside down, and spawning and fertilization occurred onto the ceiling of the nest cavity. After spawning, the male chased the female from the nest and mated with several other females. Fertilized eggs were spherical in shape, demersal, adhesive, transparent and yellow in color, measuring 1.86 mm (1.79~1.93 mm) in diameter. A mean of 17(12~22) various-sized oil globules were counted in the yolk. Granular materials formed a mass in the yolk. Fertilized eggs hatched at 256 hrs, 10 minutes after the morula stage under water temperature of $15.0{\sim}18.0^{\circ}C$. Newly hatched larvae 9.34 mm (9.02~9.69 mm. n=10) in total length (TL) had a large yolk At 14 days after hatching, larvae 11.40 mm (11.07~11.72 mm, n=10) in TL transformed to the postlarval stage. At 41 days after hatching, postlarvae of 18.42 mm (17.31~18.62 mm, n=10) in TL had reached the juvenile stage. The result of this study indicate that Cottus hangiongensis has the spawning ground in the lower stream and the amphidromous life history which is the different from that of Cottus poecilopus.

• Korean Journal of Poultry Science
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• v.31 no.4
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• pp.203-212
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• 2004

A total of 1,200 eggs obtained from 312-day-old Hy-line Brown laying breeder hens and 319-day-old Hy-line Brown commercial laying hens (600 eggs obtained from each one) at same day were used to investigate the effects of egg storage, storage temperature, and insemination of hens on the change of albumen height, Haugh unit(HU), albumen pH, shell strength, and yolk color. Eggs were stored up to 14 days after lay at $3^{\circ}C$ or $10^{\circ}C$ and sampled one day after stored and then 24 hours interval. Longer periods of storage resulted in lower albumen height and HU at both storage temperatures, but in higher albumen pH. The eggs stored at $3^{\circ}C$ were significantly (P<0.05) higher in HU and lower in albumen pH than the ones stored at $10^{\circ}C$. The eggs obtained from the non-inseminated-hens were significantly (P<0.05) higher in albumen height, HU, and albumen pH than the eggs obtained from the inseminated-hens. Whereas, the eggs obtained from the non-inseminated-hens in the mean albumen pH of eggs stored at $3^{\circ}C$ were significantly (P<0.05) higher than the ones obtained from the inseminated-hens, but the mean albumen pH of eggs stored at 10^{\circ}C did not differ each other. The mean shell strength of the eggs obtained from the inseminated-hens was significantly (P<0.05) stronger than that of the eggs obtained from the non-inseminated-hens at both storage temperatures. Albumen height and albumen pH were negatively correlated $(P<0.01\~0.001)$ in both inseminated and non-inseminated-hen's egg groups. The degree of yolk colors was not significantly changed overall of the experimental periods in both storage temperatures. The study suggests that the change of egg freshness such as albumen height and HU is relatively more associated with storage period and storage temperature than insemination or non-insemination of hens.

• Korean Journal of Poultry Science
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• v.31 no.1
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• pp.9-15
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• 2004

This study was conducted to evaluate the effects of dietary herbal plant mixture on the performance in laying hens under heat stress. One hundred ninety two 54-weeks-old ISA Brown commercial layers, were used in 56 d experimental assay. Dietary treatments included CON (control; basal diet), HPM0.05 (basal diet ＋ 0.05% herbal plant mixture), HPM0.1 (basal diet ＋ 0.1% herbal plant mixture), and HPM0.2 (basal diet ＋ 0.2% herbal plant mixture). For overall period, the hens fed with HPM0.1 and HPM0.2 diets showed lower in the hen day egg production than the hens fed with CON diet(P＜0.05). At the end of the experimental period, egg weight was heavier in HPM 0.1 treatment than in CON (P＜0.05). There were no significant differences among the treatments in egg shell breaking strength, egg shell thickness, Haugh unit, and yolk color unit. Total cholesterol concentration of yolk tended to decrease as the level of herbal plant mixture in the diet increased. Total protein of blood was higher in the hens fed with herbal plant mixture than in the hens fed with CON diet (P＜0.05). Albumin concentration of blood was increased in HPM0.05 and HPM0.1 treatments compared with CON(P＜0.05). Red blood cell (RBC) and white blood cell (WBC) concentrations in serum were increased in HPM0.1 and HPM0.2 treatments compared with CON treatment (P＜0.05). In conclusion, dietary herbal plant mixture in laying hens under heat stress adversely affected egg production but increased total protein, albumin, RBC and WBC in blood.

• Korean Journal of Poultry Science
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• v.33 no.3
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• pp.225-231
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• 2006

To investigate the effect of feeding rare earth (RE) on the performance of breeder hens a feeding trial with sixteen thousands of 158 day old Ross broiler breeder hens was conducted for thirty weeks. A mixture of RE- chlorides containing mainly La, Ce, and Pr was mixed into corn-soy based diet at two levels, 300 and 600 mg/kg, All the hens were housed in flat layer houses and the ratio of male to female was maintained at one to ten. Dietary supplementation of the of RE at a level of 300 mg/kg made the hens reach egg production peak higher by about 6% and earlier by about two weeks. As a result, it made higher hatchable egg production by 3.5%. It also reduced dramatically the mortality of both male and female breeders(P<0.05). Egg weight was slightly increased but egg qualify was not much influenced by dietary supplementation of RE. Egg albumin hight and Haugh unit were significantly improved while egg shell thickness, egg breaking strength and yolk color were similar to those of the control. Experimental results appeared to show a good possibility that egg fertility and hatchability were improved by feeding RE. Dietary supplementation of RE at a level of 300 mg/kg should be acceptable but not the 600 mg/kg level for breeder hens. Further studies on the effect of RE on egg fertility and hatchability appears to be necessary.