• Korean journal of applied entomology
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• v.44 no.1 s.138
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• pp.67-72
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• 2005

In a flour milling facility, control ability of superheating effect on Indian meal moth population was teated. Thirty adults, thirty larvae and 100 eggs were placed in a paper bag ($10{\times}20cm$) containing 100 g flour and were exposed to the temperature higher than $60^{\circ}C$ for longer than 3 hours. Mortality of the Indian meal moth (${\pm}standard error$) varied with the developmental stage; mortality (${\pm}standard error$) of egg, larvae and adult were in the range of $91.40{\pm}0.93{\sim}98.80{\pm}0.20\%,\;96.00{\pm}0.67{\sim}100\%$) and $100\%$, respectively. The position of paper bag caused differences in temperature and its exposure time to the moth during the experiment, consequently. The mortality of moth was significantly different among the bag position. Exposure of the paper bag to the temperature of higher than $60^{\circ}C$ for longer than 11 hours was not sufficient to kill all the eggs.

• Parasites, Hosts and Diseases
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• v.35 no.4
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• pp.283-290
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• 1997

A total of 32 human diphyllobothriasis cases have been reported so far in Korea, exclud- ing 21 egg-positive cases from stool examinations. Authors experienced five more human cases of DiphHllobothrium latum infection, especially infected due to eating raw flesh of redlip mullet, Lizc hoematocheiln. Five cases were neighbors residing in the Puchon area, Kyonggi-do, who ate raw mullets (L. hQematocheila) in a party in February 1996. The mul- lets were purchased at the Noryangjin fisheries market in Seoul. All of cases (2 males and 3 females) were 35 to 43 years old and healthy with the body weight range of 56-62 kg. They complained about gastrointestinal trouble and abdominal discomfort, but were in normal ranges of their hematology and urinalysis data. None revealed any sign of anemia The patients experienced natural discharge of a chain of segments before, and showed diphyllobothriid eggs in their stool specimens when they visited our laboratory. They were administered with praziquantel (15 mg/kg of body weight) and 30 g of magnesium sulfate as a purgative. Two whole worms with the scolices (310-340 cm in length; 8-13 mm in width) were expelled each from two out of five cases after anthelmintic treatment, and the others expelled the parts of strobilae without scolex. The worms were identified as D. Ictum, based on the following biological characters. external morphologies, coiling of uterus, the number of uterine loops, position of genital opening, morphologies of cirrus, cirrus sac and seminal vesicle on the histological sections, position of vagina and uterine pore , and microscopical and SEM morphologies of the eggs.

• Korean Journal of Environment and Ecology
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• v.34 no.4
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• pp.274-281
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• 2020

This study aimed to investigate the autumn spawning by bitterling (A. rhombeus) inside mussel (Unio douglasiae) and the adaptation characteristics at the Bongseocheon Stream of Mankyeonggang River. The survey was carried out between August 2015 and July 2016. The spawning season was from September to November, and 17-75 (36.2 ± 16.44) eggs were found from mature females. During the survey period, 476 mussels were collected, 129 (27.1%) of spawned A. rhombeus. Mussels that spawned eggs, embryos, and larva of A. rhombeus (46.3 ± 4.55 mm, n = 129) were larger than than those that did not spawn (42.6 ± 8.51 mm, n = 347). The appearance frequency of A. rhombeus larva before and after the formation of lens was 99.8% (n = 597) vs. 0.2% (n = 1) from October 2015 to March 2016, 25.6% (n = 23) vs. 74.4% (n = 67) in April 2016, 0% (n = 0) vs. 100% (n = 40) on May 2016. The number of eggs, embryos, and larvae of A. rhombeus inside the mussels were 1-18 (5.6 ± 3.81). The number and appearance frequency of A. rhombeus eggs, embryos, and larvae inside the mussel according to mussel gill demibranchs position were 1 (0.01 ± 0.09, n = 1) and 0.78% in the left outer demibranch, 1-18 (2.33 ± 3.31, n = 63) and 48.84% in the left inner demibranch, 1-15 (2.97 ± 3.79, n = 76) and 58.91% in the right inner demibransh, and 1-12 (0.33 ± 1.71, n = 7) and 5.43% in the right outer demibransh. The highest frequency of the developmental position of eggs, embryos, and larvae occurred 71.8% (n = 445) in lower part 3 (L3) before formation lens and 94.4% (n = 102) in L3 after formation lens, indicating that L3 was dominating position for eggs, embryos, and larvae. More eggs, embryos, and larvae of A. rhombeus were found more often in the inner demibranshs than outer demibranchs. Since A. rhombeus is a species that spawn in the autumn and thus avoids the competition with interspecific and glochidia. However, they have to spend the winter in low water temperature. Consequently, we assume that A. rhombeus have evolved toward embryonic diapause under the low water temperature before the formation of lens and spawning inside the supracranchial cavity to save the transit energy from the water space to the suprabranchial cavity after the achieving movement ability with the formation of lens.

• Korean journal of applied entomology
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• v.4
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• pp.39-46
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• 1965

The author studied on the bionomics of Oriental moth. Cnidocampa flavescens WALKER, damaging to the persimmon tree n the southern part of Korea from 1964 to 1965. The results can be summarized as follows; 1. Emergence peak period of Oriental moth was mid-June in Taegu district and eggs are deposited on the opposite side of persimmon tree leaf. Specially most of eggs are deposited on the terminal part of opposite side and peak period s also mid-June. 2. Hatched Percentage of eggs was $84.4\%$ in 1964 while $96.1\%$ in 1965 at the rearing room. Mean egg Period was $5.984\pm0.162$ in 1964 while $6.262\pm0.094$ days in 1965. Thus during two years, the egg period was about 6 days. 3. In the growth ratio of Oriental moth fed on various host plants persimmon tree, Acer negund, Hazel-wood and Platanus, the best growth ratio was shown on the leaf of Hazel-wood from 1st till 3rd instar, but, on the contrary, persimmon tree was the best from 4th till the last instar. The growth ratio of head width was also the same tendency as the body length above mentioned. Individuals fed on the leaf of platanus were dead after 20 days. 4. Oriental moth has one generation a year and molts 6 times. The first molting occurred in 5 hours after hatched, and the other moltings were done at f days intervals. After 3 days since the last molting, larvae made the non for over-winter in it. 5. As the bristles on the process of larval body are different from each position and instar, judgement of instars are possible by the counting of bristles on the body according to the Table 8. Specially the bristle of L. 2., D. 2, 3 ,8. 10. and L. 1, 3, 4, 5, 6, 7, are perfectly different from each instar. From these bristles, instars can be recognized easily. 6. Pupation of larvae in the over-wintered cocoon on the stem of persimmon tree was done in mid-May and continued will early June when emergence will take place. 7. Mean number of eggs in the ovary was $1325.5\pm2.7182$

• Korean Journal of Poultry Science
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• v.34 no.2
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• pp.151-156
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• 2007

The Function of the calcium sensing receptor (CaSR) is to control calcium levels by altering PTH (parathyroid hormone) secretion and renal calcium resorption. The influence of calcium on the basal and stimulated release of several hormones from chicken pituitary glands has been determined in vitro. The objective of this study was to identify SNP in chicken CaSR gene and to investigate the effect of the SNP on economic traits. The sequencing analysis method was used to identify nucleotide polymorphisms within chicken CaSR gene. This study identified SNP at position 1949 bp(Genebank accession No : XM_416491) in the exon 1. The SNP changed the amino acid to alanine(GCC) from serine(TCC). This SNP showed three genotypes, AA, AS and SS by digestion with the restriction enzyme NcoⅠ using the PCR-RFLP method. The A963S showed significant effect only on the first lay day (P<0.05) in Leghorn population. Leghorn with the genotype AA had significantly faster the first lay day(137.6) than the genotype AS(143.0, P<0.05). Also, the A963S showed significant effect only on the first lay day(P<0.05) and mean of egg weight(P<0.05) in KNC population. KNC with the genotypes AA ans AS had significantly faster the first lay day (151.0 and 152.6, respectively) than the genotype SS(159.4, P<0.05). And the genotypes SS had significantly heavier the mean of egg weight(50.4 kg, P<0.05) than the genotype AA ans AS (47.5 and 47.8 kg, respectively). According to result of this study, an a allele of the A963S was found to have a significant effect on the first lay day. It will be possible to use this SNP marker on selecting chicken to improve the first lay day.

• Korean journal of applied entomology
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• v.49 no.1
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• pp.11-16
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• 2010

Measurements were made on morphology of each developmental stages of the chestnut weevil, Curculio sikkimensis, reared in the laboratory and field from 2003 to 2006. The size of egg was 0.8${\pm}$0.03 mm. The escaping larvae were measured, in average, as 98 mg in body weight, 10.65 and 3.99 mm in body length and width, and 1.70 mm in head width. Pupal size of female and male was 7.01 and 6.53 mm, respectively. The fresh weight (0.343 g), body length (7.76 mm) and width (3.38 mm), and head width (1.60 mm) of female adults were significantly bigger than those (0.268 g, 7.14 mm, 3.01 mm and 1.37 mm, respectively) of male adults. Proboscis length (6.53 mm) and antennal length (5.47 mm) of female was also significantly longer than those (3.56 and 4.63 mm, respectively) of male. The larvae of C. sikkimensis overwintered for 1~3 years and their body weight, body length, and body width were decreased. The ratio between proboscis length and body length, the basipodite position attached to the proboscis, and shape of the sex organ on the abdominal end could be used to discriminate sexes.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.17 no.1
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• pp.68-80
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• 1984

Garolim Bay is not only important fishing ground but also expected area for the tidal power plant. The construction and operation of tidal power plant will make change the ecosystem of this bay. Therefore, the actual fisheries stocks should be precisely understood for the effect estimation and overall utilization of the bay after the construction of the tidal power plant. During the study period from January through December in 1981, forty-six adult fishes species, 3 species of fish egg and 25 fishes larvae species have occurred in the bay. Considering the result on monthly distribution of eggs and larvae, the inner area of the bay seems to be important as nursing ground of larvae spawned at the outside bay in winter, e. g., Ammodytes personatus, and Enedrias sp. This inner bay is also major spawning ground for many species spawning in spring and summer, e. g., Gobiidae, Konosirus punctatus, Engraulis japonica, etc. Taking into consideration the annual mean production for three years($1978{\sim}1980$), there are two major fishing seasons. The one is in May-June for Enedrias larvae stock, and the another in October-November for big eyed herring stock. For the mariculture stocks, short necked clam, oyster and laver are important species. After construction of the tidal power plant, the migratory species, i. e., larvae of Enedrias and Ammodytes personatus, Mugil cephalus, Konosirus punctatus, etc. will be directly damaged by the interuption of migration route. On the otter hand, the change of physico-chemical factors of seawater will also affect the ecosystem of the bay. Consequently, for the overall utilization of the bay after construction, the actual ecosystem including the fisheries stocks, must bs precisely revealed, and the mechanical designs, e. g., sluice position and its demension, should be also considered with these biological characters of the bay.

• Korean journal of applied entomology
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• v.58 no.3
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• pp.175-182
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• 2019

The citrus leafminer, Phyllocnistis citrella (Lepidoptera: Gracillaridae), is an important pest that decreases the productivity of citrus trees by attacking citrus leaves, and especially it causes a severe damage during the young growth stage. In this study, we investigated the distribution of eggs on the leaves of shoots according to the position and the survival rate of this pest in the field condition. The occurrence patterns of P. citrella eggs in citrus orchards were largely affected by the occurrence of shoots. Even if the adults were still being attracted in the pheromone traps, the eggs were not observed on leaves when the growth of shoots was stopped. The density of P. citrella eggs in the citrus shoots peaked at the fifth or sixth leaf from the terminal leaf, and over 90% of the eggs were found in the first to eighth leaves as a whole. In 2015 and 2016, the average survival rate of larvae surviving to pupa was 1.4%, and the rate of survival to adults was 0.2%, indicating that most of them died. Furthermore, we have discussed the management strategy of P. citrella considering the distribution of eggs and the larval survival rate in the citrus shoots.

• Cross-Cultural Studies
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• v.52
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• pp.277-312
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• 2018

First-order cybernetics during the 1940s and 1950s aimed for control of an observed system, while second-order cybernetics during the mid-1970s aspired to address the mechanism of an observing system. The former pursues an objective, subjectless, approach to a system, whereas the latter prefers a subjective, personal approach to a system. Second-order observation must be noted since a human observer is a living system that has its unique cognition. Maturana and Varela place the autopoiesis of this biological system at the core of second-order cybernetics. They contend that an autpoietic system maintains, transforms and produces itself. Technoscientific recreation of biological autopoiesis opens up to a new step in cybernetics: what I describe as third-order cybernetics. The formation of technoscientific autopoiesis overlaps with the Fourth Industrial Revolution or what Erik Brynjolfsson and Andrew McAfee call the Second Machine Age. It leads to a radical shift from human centrism to posthumanity whereby humanity is mechanized, and machinery is biologized. In two versions of the novel Demon Seed, American novelist Dean Koontz explores the significance of technoscientific autopoiesis. The 1973 version dramatizes two kinds of observers: the technophobic human observer and the technology-friendly machine observer Proteus. As the story concludes, the former dominates the latter with the result that an anthropocentric position still works. The 1997 version, however, reveals the victory of the techno-friendly narrator Proteus over the anthropocentric narrator. Losing his narrational position, the technophobic human narrator of the story disappears. In the 1997 version, Proteus becomes the subject of desire in luring divorcee Susan. He longs to flaunt his male egomaniac. His achievement of male identity is a sign of technological autopoiesis characteristic of third-order cybernetics. To display self-producing capabilities integral to the autonomy of machinery, Koontz's novel demonstrates that Proteus manipulates Susan's egg to produce a human-machine mixture. Koontz's demon child, problematically enough, implicates the future of eugenics in an era of technological autopoiesis. Proteus creates a crossbreed of humanity and machinery to engineer a perfect body and mind. He fixes incurable or intractable diseases through genetic modifications. Proteus transfers a vast amount of digital information to his offspring's brain, which enables the demon child to achieve state-of-the-art intelligence. His technological editing of human genes and consciousness leads to digital standardization through unanimous spread of the best qualities of humanity. He gathers distinguished human genes and mental status much like collecting luxury brands. Accordingly, Proteus's child-making project ultimately moves towards technologically-controlled eugenics. Pointedly, it disturbs the classical ideal of liberal humanism celebrating a human being as the master of his or her nature.

• Korean Journal of Plant Tissue Culture
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• v.24 no.1
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• pp.1-35
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• 1997

Fertilized egg, by successive cell divisions, differentiates into different tissues and organs with various structures and functions. Different cells and tissues contain different proteins, products of selective gene expression. Not all the genes in any genomes are equally active, temporal and spatial gene expression being the general rule. Present paper attempts to review the tanscriptional mechanisms or the initiations of transcription from several angles. In some of the organisms the genes in the process of transcription or the genes in the inactive state can be seen under the light microscope. Some bands of Drosophila polytene chromosomes may exhibit a swollen or puff appearance under certain conditions. A puff, unfolded or decondensed form of chromomere, represents sets of intense transcriptional activity or RNA synthesis. The heterochromatic X chromosome whose genes remain inactive in the female mammals can be visualized as a dark staining structure called Barr body, Configuration of chromatin differs between transcribed and nontranscribed chromatin. Modification to the chromatin facilitates RNA synthesis. The movement of large polymerase molecule along the DNA would probably be facilitated if some modifications of the chromatin configuration is effected. Methylation of cytosines in CG sequences is associated with inactive genes. Methylation can play a role in determination of mammalian cells during embryogenesis. Demethylation is necessary for the gene to be expressed during development A histone modification that is also known to be correlated with transcriptional capacity of chromatin is acetylation of the lysine residues of the core histones. Chromatin containing a high level of histone acetylation is very sensitive to DNase 1. For the transcription to occur TBP must first bind to the TATA box. Another TF, TF IIB, then binds to the promoter-TBP complex, facilitating the access of RNA polymerase to the transcription initiation site. As recently as eight years ago researchers assumed that histones were irrelevant to the regulation of gene expression. Histones combine with the DNA to form nucleosome of the chromatin. Histones are vital participant in gene regulation. Histone and basal factors compete for access to TATA box. When DNA is exposed to basal factors before histones are introduced, the basal factors assemble on TATA boxes preventing the access of histones, allowing transcription to occur, for transcription to begin, activator protein at the upstream activation sequence or enhancer must interact with the tail of histone H4 at TATA box and cause the histone role particle to dissociate from the TATA box leading to partial breakup of the histone core particle and allowing the basal factors to bind to the TATA box. New concept of genomic flux in contrast to the old concept of static genome has been developed based on the powerful new molecular techniques. Genomic changes such as repetitive DNAs and transposable elements, it is assumed but not yet proved, may affect some of the developmental patterns that characterize particular cells, tissues, organs, and organisms. In the last decade or so remarkable achievement have been made in the researches of the structures and functions of TFs and the specific target sequences located in promoters or enhancers where these TFs bind. TFs have independent domains that bind DNA and that activate transcription. DNA binding domain of TFs serves to bring the protein into the right location. There are many types of DNA binding domains. Common types of motifs can be found that are responsible for binding to DNA. The motifs are usually quite short and comprise only a small part of the protein structure. Steroid receptors have domains for hormone binding, DNA binding, and activating transcription. The zinc finger motif comprises a DNA binding domain. Leucine zipper consist of a stretch of amino acids with a leucine residue in every seventh position Two proteins form a dimer because they interact by means of leucine zippers on similar α-helical domain. This positions their DNA binding basic domains for interaction with the two halves of a DNA sequence with dyad symmetry of TGACTCA, ACTGAGT.