• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2003년도 생물공학의 동향(XIII)
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• pp.521-524
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• 2003

An effective purification method was developed for producing Homoharringtonine (HHT), to guarantee high purity and yield from Cephalotaxus koreana. This process was a simple and efficient procedure, for the isolation and purification of HHT form the biomass of Cephalotaxus koreana, consisting of extract, adsorbent treatment, precipitation and followed by a chromatography. The extraction, adsorbent treatment and precipitation in pre-purification process allows for rapid and efficient separation of HHT from many compound and dramatically increases the yield and purity of crude HHT for HPLC purification steps compared to alternative processes. This purification processes serves to minimize solvent usage, size, and complexity of the operations for HHT purification.

• Journal of Ginseng Research
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• 제22권4호
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• pp.284-288
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• 1998

This study was carried out to establish a new efficient method for isolation and purification of ginsenosides. Silica gel column chromatography, having been used for the isolation of ginsenosides, is advantageous to obtain a large amount of ginsenosides. However, it has a disadvantage to isolate ginsenosides to their highest purity. In addition, normal-or reverse-phase HPLC method thus far reported is confined to quantitative analysis. Especially, it has not been possible to isolate racemic 20(S)- and 20(R)-ginsenoside Rg2. In this experiment, isolation and purification of ginsenosides were accomplished by Diaion HP-20 adsorption chromatography, silica gel column chromatography, recrystalization and Prep. HPLC with or without Prep. TLC. From this study, we could establish a new efficient method for isolation and purification of 9 major and/or minor ginsenosides.

• KSBB Journal
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• 제15권4호
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• pp.342-345
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• 2000

식물세포배양으로부터 peroxidase를 대량 생산하기 위한 분리/정제 공정 으로서 세포를 파쇄하고 크로마토그래피 전처리로서 활성백토를 적용하였다. 활성백토는 미세한 세 포 조각 뿐만 아니라 여려가지 불순불을 선택적으로 흡착 하는 성질을 나타내었으며, 이를 통하여 효과적으로 정제 공정을 진행할 수 있었다 흡착을 통한 전처리 후 한외여 과장치를 이용하여 농축을 실시 하였으며, DEAE-Sepharose F FF를 이용한 크로마토그래피를 통해 정제가 이루어졌다. 활성을 나타내는 용액을 탈염, 농축 후 동결 건조하였다. 이 공정은 상업화에 필요한 대량 정제를 가능하게 하였으며 수율과 정제비용 측면에서 상당히 경제적인 공정임을 입증하였다, 활성백토를 이용한 흡착공정은 다른 효소 및 단백질의 경제적인 대량정제에 적용될 수 있으리라 기대된다.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2005년도 생물공학의 동향(XVII)
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• pp.566-569
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• 2005

한국산 개비자나무로부터 새로운 개념의 homoharringtonine 분리 및 정제 공정을 개발하였다. 메탄올을 사용한 4회 반복 추출에 의해 biomass로부터 대부분(>99%)의 homoharringtonine을 회수 할 수 있었다. 흡착 공정에서는 활성 백토(active clay)를 사용하여 추출물에 포함되어 있는 식물유래 타르, 왁스 성분을 효과적으로 제거하였다. Silica gel low-pressure chromatography공정을 통해서 순도 52% 이상의 homoharringtonine을 얻었으며, HPLC 공정을 통해 고순도 및 고수율의 homharringtonine을 정제할 수 있었다. 정제된 homoharringtonine의 분자량 및 구조분석을 수행한 결과 표준물질(Homharringtonine)과 동일 물질임을 확인할 수 있었다.

• 한국가시화정보학회지
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• 제20권3호
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• pp.42-48
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• 2022

The shortage of available freshwater is a major global issue worldwide and an increasing demand for clean water requires efficient water purification strategies. Here we describe a method to drastically increase the efficiency of a microreactor for photocatalytic water purification. To find out how the shape of the catalyst affects water purification, nanostructured catalysts of different structures, such as dense film, nanorod, and nanohelix, are prepared and their water purification characteristics are analyzed. Compared to the flat catalyst, the nanostructured catalyst showed a distinct ability in its pollutant degradation, but the detailed structural variation does not significantly affect the water purification. To further increase efficiency, we apply a micromixer to nanorod-based microreactor, which allows even enhanced mass transfer. This enables the solution of the water purification problem and greatly contributes to the industries where the efficiency of photocatalytic activity has attracted extensive interest.

• 산업기술연구
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• 제31권A호
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• pp.135-145
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• 2011

This study was conducted to analyze the cost effectiveness in line with total phosphorus standard enforcement of public sewage treatment facilities. The additional cost for the total phosphorus removal process was calculated to analyze the cost effectiveness of the advanced water purification process. The analysis results showed that the T-P removal by coagulation sedimentation was more efficient than the advanced water purification facilities in terms of facilities investment cost, and if the coagulation filteration was used for T-P removal, the activated carbon process among the advanced water purification techniques was more efficient in terms of facilities investment cost. In this study, the effects of the T-P removal process that will be additionally introduced according to the tightening of the effluent T-P standard were analyzed within a limit. The actual benefits of improved T-P concentration in the water source will provide diverse values, including the leisure water, environment improvement water, eco-system in the water source and industrial water, in addition to the advanced water purification.

• 한국축산식품학회지
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• 제20권1호
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• pp.36-43
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• 2000

$\beta$-cyclodextrin adsorption and saponification methods were applied to isolate and purify cholesterol from the by-product of the low-cholesterol egg yolk product. They by-product was prepared from processing low-cholesterol egg yolk followed by extracting with chloroform to remove $\beta$-cyclodextrin and concentrated to 3,069 mg% cholesterol. When $\beta$-cyclodextrin method between two purification methods was applied, 50% ethanol as a solvent showed higher cholesterol concentration of 5.82% rather than the other solvents. Repeated purification of 3 times could not improve the cholesterol concentration significantly(p<0.05). In case of purification using saponification method, hexane as a solvent for extraction of unsaponificated materials was more efficient to increase cholesterol concentration than chloroform and ether. 60 times(v/w) saponification solution (95% ethanol:33% KOH = 94:6) of sample weight was most effective to increase the cholesterol concentration of 35.7%. Repeated purification process by saponification method could increase cholesterol concentration to 95.7% by 4 times repetition.

• 한국응용과학기술학회지
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• 제24권4호
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• pp.383-392
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• 2007

This paper is studied on the efficient purification process of dimethyl carbonate (DMC) from the melt layer crystallization combining crystallization process, sweating process and distillation recovery process. Purity and yield of DMC crystal depended mainly on the crystallization temperature, cooling rate, sweating termperature sweating rate. Through the optimization of crystallization and sweating operation, DMC crystal can be upgraded to very high purity over 99.9% and high yield over 85%.

• Journal of Microbiology and Biotechnology
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• 제11권2호
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• pp.204-210
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• 2001

Paclitaxel can be produced in high yield and with a high degree of purify from plant cell cultures of Taxus chinensis. The complete purification method was systematically established and described. This method was an efficient procedure for the purification of paclitaxel from crude paclitaxel, consisting or reverse-phase chromatography, followed by a normal-phase chromatography. The two-stage HPLC purification scheme serves as an effective and economical approach for resolving paclitaxel from complex mixtures of taxoids, with high purify (>99%) and low impurities (<0.1%). The process is readily scalable to a pilot plant and eventually to a production environment where multikilogram quantities of material are expected to be produced. The process has been optimized to minimize solvent usage, complexity, and operating costs.

• Tribology and Lubricants
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• 제38권4호
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• pp.121-127
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• 2022

This study presents the development of an integrated oil purification system consisting of moisture removal, oil flushing, and oil filtering devices. In this system, the oil flushing device is combined with a micro-bubble generator. Oil purification is necessary for ensuring the high performance of the lubricant through the efficient removal of contaminants and thus enables good maintenance of mechanical systems. The developed purification system removes moisture, varnish, and solid particles. Moreover, during oil purification, the oil flushing device separates foreign materials and contaminants remaining in the lubricating oil piping or mechanical systems. The microbubble generator, which is combined with the oil flushing device, can separate harmful contaminants, such as sludge, wear particles, and rust, from piping or lubrication systems through the cavitation effect. Moisture is removed using a double high-vacuum chamber, while sludge and varnish are removed via electro-absorption using a high-voltage generator. Additionally, the total maintenance cost of the system is reduced through the use of domestically fabricated cartridge filters composed of glass fiber and cellulose. The heater, which maintains the temperature of the lubricant at 60℃, can process 41,000 L of lubricant simultaneously. Multiple tests confirmed that the proposed integrated purification system exhibits good performance in oil flushing and removal of water and varnish.