• Journal of Microbiology and Biotechnology
• /
• v.14 no.6
• /
• pp.1239-1248
• /
• 2004

The methylotrophic yeast Hansenula polymorpha has been extensively studied as a model organism for methanol metabolism and peroxisome biogenesis. Recently, this yeast has also attracted attention as a promising host organism for recombinant protein production. Here, we describe the fabrication and evaluation of a DNA chip spotted with 382 open reading frames (ORFs) of H. polymorpha. Each ORF was PCR-amplified using gene-specific primer sets, of which the forward primers had 5'-aminolink. The PCR products were printed in duplicate onto the aldehyde-coated slide glasses to link only the coding strands to the surface of the slide via covalent coupling between amine and aldehyde groups. With the partial genome DNA chip, we compared efficiency of direct and indirect cDNA target labeling methods, and found that the indirect method, using fluorescent-labeled dendrimers, generated a higher hybridization signal-to-noise ratio than the direct method, using cDNA targets labeled by incorporation of fluorescence-labeled nucIeotides during reverse transcription. In addition, to assess the quality of this DNA chip, we analyzed the expression profiles of H. polymorpha cells grown on different carbon sources, such as glucose and methanol, and also those of cells treated with the superoxide­generating drug, menadione. The profiles obtained showed a high-level induction of a set of ORFs involved in methanol metabolism and oxidative stress response in the presence of methanol and menadione, respectively. The results demonstrate the sensitivity and reliability of our arrays to analyze global gene expression changes of H. polymorpha under defined environmental conditions.

• Cartoon and Animation Studies
• /
• s.14
• /
• pp.151-164
• /
• 2008

Cross platform game has brought about the expansion of game market, which results in technology innovation overcoming the limit of game consumption. The new model integrates both off and online game services. Gamers can now enjoy game service regardless of age, time, and space. If the technology evolution model of digital contents like cross-platform game engine can provide contents for several platform at the same time, the interactive service can be utilized into maximum level. It is also necessary to allocate, switch data as well as to innovate the transmission technology of data according to each platform. Providing the same contents for several platform as many as possible can be the most suitable strategy to enhance the efficiency and profits. However if the interactive service can be accomplished completely, the development of data switching technology and distribution should be made. To be a leader in the next digital contents market, one should develop the network engine technology which can embody the optimization of consumption in the interactive network service.

• Journal of Energy Engineering
• /
• v.21 no.4
• /
• pp.402-410
• /
• 2012

A two-loop Rankine cycle for engine waste heat recovery of gasoline vehicle has been investigated. Water-steam cycle as a high-temperature (HT) loop for exhaust gas heat recovery and R-134a cycle as a low-temperature (LT) loop for both heat recovery of the engine coolant and the residual heat from the HT loop were considered. Energy and exergy analysis was performed to investigate the performance of the system. Because two volumetric expanders are used for the HT and LT loop, the sizes of two expanders are very important for the optimization of the system. The effects of pressure ratio of the HT loop, considering the size of the HT expander, and the condensation temperature of LT loop on the performance of the system at a target engine condition were investigated. This study shows that about 20% of additional power from the engine waste heat recovery can be obtained at the target engine condition.

• Applied Chemistry for Engineering
• /
• v.9 no.7
• /
• pp.1043-1046
• /
• 1998

Aluminium based inorganic flocculants are extensively used in this country in the removal of fine particles present in the raw water for the production of drinking water. These inorganic flocculants, however, have potential hazard of high residual aluminium ions in the treated waters, which is known to be one of the reasons of alzheimer's disease. Inorganic flocculants alone are sometimes incapable of treating water when there are excessive turbidity in the raw water sources due to flooding. A polyamine type polymeric flocculant has long been used to treat raw water in the drinking water production in the European countries and United State of America. The optimum reaction conditions such as mole ratio of epichlorohydrin(EPI) to dimethylamine(DMA), reaction temperature and time for each stage for the pilot scale preparation of polyamine from EPI-DMA was studied in this work. The variation of intrinsic viscosity and flocculating efficiency in the water treatment of the synthesized polyamines were evaluated. The residual materials after polymerization reaction were analyzed by gas chromatography to study the effect of variation of reaction conditions.

• Applied Biological Chemistry
• /
• v.41 no.6
• /
• pp.465-470
• /
• 1998

For the need of bio-degradable flocculant in stage of wastewater treatment, some cultural conditions of bioflocculant production were optimized with Aeromonas hydrophila KH-54. About 260 strains of type culture and bacteria isolated from marsh, pond, activated sludge, etc were examined for their ability to flocculate kaolin particles and swine wastewater. Among them, KH-54 showed the highest flocculating activity and was identified as Aeromonas hydrophila according to the cultural, morphological and physiological properties. The maximum production of the flocculant secreted by Aeromonas hydrophila KH-54 was observed in culture medium containing 2.0% mannitol, 0.05% ammonium chloride, 0.02% potassium phosphate dibasic, 0.01% $MgSO_4{\cdot}7H_2O$ and 0.05% yeast extract at initial pH 7.0 when cultured on rotary shaker controlled at $25^{\circ}C$ and 150 rpm. Under the optimized condition, the flocculating ability reached to 770 units/ml of kaolin flocculating activity and 81% of NTU removal efficiency against swine wastewater after 4 days cultivation. The bioflocculant was also effective on various organic wastewaters other than swine wastewater, showing NTU removal rate ranging from 92% to 34%.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.19 no.2
• /
• pp.695-701
• /
• 2018

Research into exhaust heat recovery has been actively carried out to improve the thermal efficiency of internal combustion engines. In this study, the performance of thermoelectric generation from exhaust heat recovery for motorcycle engines was analyzed by 1-D thermo-fluid simulation. GT-SUITE, which was developed by Gamma Tech., was used for the simulation of the internal combustion engine and thermoelectric generation system. The basic performance of the engine was analyzed in the range of engine speed of 1000~7000 rpm and engine load of 0~100%. The ratio of exhaust heat energy to fuel chemical energy was found to be about 40~60%. A combined simulation of the engine model and thermoelectric generation model was carried out to analyze the voltage, current and power generated by the thermoelectric material. The generation characteristics of the thermoelectric material was dominantly affected by the exhaust gas temperature. The maximum generated power of the current thermoelectric generation system was found to be about 2.2% of the total exhaust heat energy. The design optimization of the thermoelectric generation system will be carried out to maximize its power generation and economic feasibility.

• Journal of Advanced Navigation Technology
• /
• v.20 no.5
• /
• pp.446-452
• /
• 2016

The aircraft has to be considered for safety very importantly because of peculiarity of flight in the air, so it should be retained through proper inspection and maintenance not only in production phase but also in operating phase. Recently, it is using the latest technology as engineering approach not depending on human factor to determine on maintenance needs, and domestic production rotary aircraft also has the health & usage monitoring system to measure and to monitor major components. However, continued vibration exceedance phenomenon occurred in production and operation phase because of inappropriate thresholds, and it confirmed as false alarm which is not necessary to repair. In this paper, it is described that operational concept of HUMS, and especially it contains a study result for efficiency of aircraft operation and ultimately the improvement of flight safety by optimizing HUMS thresholds to determine efficiently necessity of maintenance under limited conditions and by establishing inspection/maintenance procedures when the re-designated thresholds exceedance occurred.

• Microbiology and Biotechnology Letters
• /
• v.45 no.2
• /
• pp.149-154
• /
• 2017

To increase the efficiency of esterase production by Bacillus, high cell-density culture of recombinant Escherichia coli through fed batch fermentation was tested. Cells were cultured to $OD_{600}$ of 76 (35.8 g/l DCW) with dissolved oxygen level controlled to least above 30% air saturation by supplying pure oxygen. Cells were cultured to an $OD_{600}$ of 90 (42.4 g/l DCW) with glucose feeding controlled to at least 1 g/l. However, the cells reached stationary phase at the late stage of culture, despite glucose being supplied. Cells were cultured to an $OD_{600}$ of 185 (87.3 g/l DCW) by supplying additional medium with fortified yeast extract. To increase the productivity of the recombinant protein, cell growth and esterase productivity based on induction time were evaluated. Late exponential phase induction for esterase production in fed batch fermentation resulted in maximum optical density $OD_{600}$ of 190 (89 g/l DCW) and maximum esterase activity of 1745 U/l, corresponding to a 5.8-fold enhancement in esterase production, compared to the early exponential phase induction. In this study, we established fermentation methods for achieving maximum production of Bacillus-derived esterase by optimizing IPTG induction time in high-cell density culture by supplying pure oxygen and a nitrogen source.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.18 no.1
• /
• pp.688-695
• /
• 2017

This paper deals with characteristics analysis of a permanent magnet (PM) linear generator using analytical methods for wave energy harvesting. The wave energy is carried out from the movement of a yo-yo system. A linear generator using permanent magnets to generate a magnetic force itself does not require a separate power supply and has the advantage of simple maintenance. In addition to the use of a rare earth, a permanent magnet having a high-energy density can be miniaturized and lightweight, and can obtain high energy-conversion efficiency. We derived magnetic field solutions produced by the permanent magnet and armature reaction based on 2D polar coordinates and magnetic vector potential. Induced voltage is obtained via arbitrary sinusoidal input. In addition, electrical parameters are obtained, such as back-EMF constant, resistance, and self- and mutual-winding inductances. The space harmonic method used in this paper is confirmed by comparing it with finite element method (FEM) results. These facilitate the characterization of the PM-type linear generator and provide a basis for comparative studies, design optimization, and machine dynamic modeling.

• Journal of Korean Society of Environmental Engineers
• /
• v.31 no.5
• /
• pp.364-370
• /
• 2009

With time, the stable management of turbidity is becoming more important in the water treatment process. So optimization of flocculation is important for the improvement of the sedimentation efficiency. we evaluated the hydrodynamic behavior in the rotation direction (clock-wise, counterclock-wise) of the flocculator in the flocculation basin using Computational Fluid Dynamics (CFD). The results of the CFD simulation, in cases where flocculators rotate in a clockwise direction, a stronger flow is formed near the surface of the water where the rotating direction and current of flow correspond. The variance and standard deviation of the flux are about 8.5 and 2.9 respectively. In contrast, in the case of a counterclockwise direction, a stronger flow is formed near the bottom of the basin. The variance and standard deviation of the flux are about 5.3 and 2.3, respectively. The effluent flux is affected more by the third flocculator spin than the first and second flocculator spins. The third flocculator spinning in the counterclockwise direction is better for the uniform flow of the sedimentation basin than the third flocculator spinning in the clockwise direction