• 대한추나의학회지
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• 제3권1호
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• pp.97-109
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• 2002

Objectives : Electroacupuncture(EA) has been suggested as a treatment for stroke rehabilitation. But whether, how much, by what mechanism and when it is effective has not been answered satisfactorily. Therefore it is important to critically review clinical trials and laboratory researches about EA for stroke rehabilitation. Subjectives : We researched various recent sources of EA for stroke rehabilitation such as medical journals and especially tried to review methodologically best randomized controlled trials(RCTs). Results and Conclusions : 1) EA increases brain plasticity, activity, blood flow and secretion of neuropeptides in CNS. 2) EA is significantly effective at the case that more than half of the neural motor pathway is reserved. 3) The acupoints, frequncy and intensity of EA should be determined by patient-specific symptoms of stroke. 4) More studies is needed for merdian functions for stroke rehabilitation.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 2003년도 Annual Meeting of KSAP : International Symposium on Pharmaceutical and Biomedical Sciences on Obesity
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• pp.117-117
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• 2003

PPARs (peroxisome proliferator activated receptors) are member of nuclear hormone receptors superfamily. Activations of PPARs upon binding with ligands modulate glucose metabolite, differentiation of adipocyte, inflammation response, and so on. Thiazolidinedione analog is one of potential antidiabetic drug that binds and activates PPARν selectively and enhances insulin sensitivity. In an effort to develop novel and effective antidiabetic thiazolidindione analogs, syntheses of benzoxazole and benzothiazole-linked thiazolidinedione analogs were performed via coupling reaction of benzoxazolylalkylaminoethanol with hydroxybenzylthiazolidinedione to develop novel and effective antidiabetic thiazolidindiones. All compounds were evaluated their biological potency by PPARν transactivation assay and revealed the similar potency with Troglitazone. However, lengthening of N-alkyl substituent did not seem to be beneficial for the activity.

• Animal cells and systems
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• 제15권1호
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• pp.19-27
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• 2011

Hepatocytes, the major epithelial cells of the liver, maintain their morphology in culture dishes coated with extracellular matrix (ECM) components such as collagen and fibronectin or biodegradable polymers (e.g. chitosan, gelatin). In these coated dishes, survival of cells and maintaining of liver-specific functions may increase. The aim of this study was to determine a suitable, cost-effective and simple system for hepatocyte isolation and culture which may be useful for various applications such as in vitro toxicology studies, hepatocyte transplantation and bioartificial liver (BAL) systems. In order to obtain primary cultures, hepatocytes were isolated from liver by an enzymatic method and cultured on plates coated with collagen, chitosan or gelatin. Collagen, gelatin-sandwich and gelatin-cell mixture methods were also evaluated. Morphology and attachment of the cells were observed by inverted microscope and scanning electron microscope (SEM). An MTT assay was used to determine cell viability and mitochondrial activity.

• Journal of Microbiology
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• 제37권4호
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• pp.243-247
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• 1999

Effects of divalent cations on self-splicing inhibition by the antibiotic spectinomycin of the phage T4 thymidylate synthase intron (td) have been investigated. $Ca^{2+}$ ion at 1mM concentration suppressed splicing inhibition of spectinomycin by 10% and 50 ${\mu}M\;Co^{2+}$ ion also suppressed splicing inhibition of specinomycin by 10%. $Mg^{2+}$ ion at 6 mM concentration decreased splicing inhibition of spectinomycin by 42% while $Mn^{2+}$ ion decreased the splicing inhibition by 10%. $Zn^{2+}$ ion at 10 uM concentration lowered the splicing inhibition by spectinomycin of 15%. Of all divalent cations tested, $Mg^{2+}$ ion was the most effective in suppressing splicing inhibition by specinomycin whereas $Ca^{2+}$ ion was the least effective. The results suggest that spectinomycin may interact with specific and functional $Mg^{2+}$-binding sites within intron RNA that lead to a displacement of $Mg^{2+}$ essential for catalytic activity.

• 한국전문물리치료학회지
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• 제24권3호
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• pp.47-56
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• 2017

Background: Given the potential benefits of wellness programs promoting physical activity of the community-dwelling frail elderly, it is recommended that comprehensive wellness programs combined with the component of physical activity. This may improve overall health and potentially lower the health care cost of the frail elderly. In general, the frail elderly residing in community or those after being discharged from hospitals are often committed to enhancing the status of participation in physical activity. Objects: The purposes of this study were to identify specific shortcomings of current wellness programs as part of continuum of community rehabilitation services and to propose alternatives for the care of the community-dwelling frail elderly. Through this study, geriatric health care professionals may be able to implement assessments and programs to successfully promote an effective continuum of care for the frail elderly. Methods: Article reviews were summarized and evaluated. Results: A model elaborating the relationship between components of successful wellness program and participation in physical activities for the community-dwelling frail elderly are recommended. First, periodic monitoring the levels of physical activity by the use of online measurement system should be considered. Second, individualized adaptive technologies for selecting optimal physical activities for the elderly may be better fit to individuals' current status of physical activity. Conclusion: The current status of physical activity in community-dwelling frail elderly can be monitored by online assessment systems. Through the innovative measurement system, elderly may assess his/her physical activity status overtime, select optimal physical activities matching the status, and create the elderly's own adaptive wellness programs that match to the status while residing in his/her community.

• Animal cells and systems
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• 제2권4호
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• pp.529-538
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• 1998

In order to analyze the intracellu1ar localization of specific RNA components of ribonucleoproteins (RNP) in Xenopus oocytes, a modified protocol of whole-mount in situ Hybridization is presented in this paper, Mitochondria specific 12S rRNA probe was used to detect the amplification and distribution of mitochondria in various stages of the oocyte life cycle, and the results were found to be consistent with previously known distribution of mitochondria. The results with other specific probes (U1 and U3 small nuclear RNAs, and 5S RNA) also indicate that this procedure is generally effective in localizing RNAs in RNP complexes even inside organelles. In addition, the RNA component of RNase MRP, the RNP with endoribo-nuclease activity, localize to the nucleus in various stages of the oocyte life cycle. Some of MRP RNA, however, were found to be localized to the special population of mitochondria near the nucleus, especially in the active stage of mitochondrial amplification. It suggests dual localization of RNase MRP in the nucleus and mitochondria, which is consistent with the proposed roles of RNase MRP in mitochondrial DNA replication and in rRNA processing in the nucleolus.

• Archives of Pharmacal Research
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• 제25권3호
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• pp.229-239
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• 2002

Recent progress in understanding the molecular basis of cancer brought out new materials such as oligonucleotides, genes, peptides and proteins as a source of new anticancer agents. Due to their macromolecular properties, however, new strategies of delivery for them are required to achieve their full therapeutic efficacy in clinical setting. Development of improved dosage forms of currently marketed anticancer drugs can also enhance their therapeutic values. Currently developed delivery systems for anticancer agents include colloidal systems (liposomes, emulsions, nanoparticles and micelles), polymer implants and polymer conjugates. These delivery systems have been able to provide enhanced therapeutic activity and reduced toxicity of anticancer agents mainly by altering their pharmacokinetics and biodistribution. Furthermore, the identification of cell-specific receptor/antigens on cancer cells have brought the development of ligand- or antibody-bearing delivery systems which can be targeted to cancer cells by specific binding to receptors or antigens. They have exhibited specific and selective delivery of anticancer agents to cancer. As a consequence of extensive research, clinical development of anticancer agents utilizing various delivery systems is undergoing worldwide. New technologies and multidisciplinary expertise to develop advanced drug delivery systems, applicable to a wide range of anticancer agents, may eventually lead to an effective cancer therapy in the future.

• 한국안전학회지
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• 제30권6호
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• pp.132-138
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• 2015

This study examined the relative effects of feedback frequency and specificity of Eco-IVIS(eco in-vehicle information system) on the fuel-efficiency and workload. Eighty participants randomly assigned into four experimental groups (high frequency/specific, high frequency/global, low frequency/specific, and low frequency/global feedback) and they drove 16.4Km motorway under the each feedback condition. The dependent variable were fuel efficiency and Drive Activity Load Index which measured participants' subjective ratings of driving workload. The results showed that high frequent feedback was more effective for increasing fuel-efficiency than low frequent feedback, however, there was no significant difference of fuel-efficiency between specific and global feedback. Although, overall DALI score was comparable among four experimental conditions, visual demand score was significant higher under the high frequent feedback condition than low frequent feedback.

• 대한치료과학회지
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• 제10권2호
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• pp.21-30
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• 2018

Objective: The purpose of this study was to investigate questions and instructions for internal feedback effects on functional recovery and task performance while chronic stroke patients practised task-specific training. Method: Twenty-four chronic stroke patients were randomly divided into two groups; when patients performed same tasks, one was treated using questions and the other using instructions for internal feedback Both lasted 30 minutes, 5 times a week for 8 weeks. Outcome measures included Erasmus MC Modifications to the Nottingham Sensory Assessment (EmNSA), Measurement Properties of the Motor Evaluation Scale for Upper Extremity in Stroke patients (MESUPES), Chedoke Arm and Hand Activity Inventory (CAHAI), Korean version of Modified Barthel Index (K-MBI). Results: There were no significant differences between the two groups in EmNSA and K-MBI(p>.05). But, in MESUPES and CAHAI, there was significant difference between the two groups(p<.05). Conclusion: In this study, questions for internal feedback during task-specific training are more effective in improving upper extremity motor function and task performance than instructions for internal feedback.

• International Journal of Computer Science & Network Security
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• 제22권11호
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• pp.177-183
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• 2022

Thanks to the informatization of society, complex and high-tech devices are being introduced in all areas of human life, and the latest technologies are being actively improved in the modern, globalizing world. The article deals with the issues of using information and communication technologies in legal activities. It also covers the main types of such activities: law-making, law enforcement, and interpretive activity. Since there is an increase in the flow and accumulation of legal information, it is practically impossible to use traditional methods of working with legal information. The article considers and analyzes the role of information and communication technologies in modern legal activity. It is necessary to reveal the principles, concepts, conditions, and factors of their development and develop theoretical and practical recommendations for the use of such technologies in order to solve legal tasks. The authors of the article raise the issues of increasing the efficiency of legal activity, as well as the integration of information technologies into practical legal activity and their use for collecting, storing, searching, and issuing legal and reference information. Much attention is paid to the specific use of automated data banks and information retrieval systems in legal practice that ensure the accumulation, systematization, and effective search for legally important information. The development of such technologies leads to the creation of comfortable conditions for a lawyer in the course of their professional activity. Currently, legal activity cannot exist without telecommunication technologies, legal reference systems, and electronic programs. The authors believe that due to the use of the latest information technologies, the time for making legal decisions has significantly accelerated, the process of searching and systematizing evidence has been worked out, and it has become possible to quickly and efficiently find information on adopted laws and legal acts.