• 생물환경조절학회지
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• 제23권4호
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• pp.329-336
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• 2014

저온은 식물 생장을 저해하는 주된 요인이며 병원균에 대한 감수성을 증가시킨다. 그러므로 식물체에서 스트레스 내성을 증대시키는 것은 불리한 환경 조건에서 살아남기 위한 중요한 전략이다. 본 실험의 목적은 고추 묘에서 저온 내성과 식물병 발생에 대한 외생 살리실산(SA)과 일산화질소(NO) 처리의 효과를 밝히는 것이다. 정식 후 23일 동안 고추 묘(Capsicum annuum L. '기대만발')는 온도 $20/25^{\circ}C$(낮/밤), 광주기 15시간, 광도 $145{\pm}5{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$의 정상적인 생육환경에서 자랐다. 1주일에 2번 계면활성제 0.1%를 포함한 SA와 NO 3mL을 고추 묘에 각각 분사해주었다. 처리 후 고추 묘는 암 상태에서 6시간 동안 $4^{\circ}C$ 저온에 노출시킨 후 정상적인 생육환경에서 2일 동안 회복시켜주었다. 저온 스트레스에 대한 식물 내성을 평가하기 위해 저온 처리 후 생육특성, 엽록소 형광 값, 세포막 투과성을 측정하였다. 총 페놀릭 농도와 항산화도는 실험하는 동안 측정하였다. 또한, 고추의 점무늬병과 풋마름병 발생 정도도 조사하였다. 저온 처리 전 후를 비교하여 대조구 고추묘에서는 저온에 의해 상대적으로 많은 수분을 손실하여 건물율이 높지만 SA와 NO 처리 된 고추 묘는 비슷한 건물율을 유지하였다. 저온 처리 후 대조구에 비해 SA와 NO 처리구의 전해질 유출 값은 더 낮았다. 저온 처리 동안 SA와 NO 처리구의 엽록소 형광값은 약 0.8 수준으로 유지하였지만 대조구는 빠르게 감소하였다. 화학적 처리 동안 SA 처리구의 총 페놀릭 농도와 항산화도는 NO 처리구보다 높았다. 또한 저온 처리 후 대조구와 NO 처리구의 총 페놀릭 농도는 증가하였다. 고추에서 풋마름병에 대한 저항성은 SA가 보다 효과적이었다. 본 실험의 결과는 SA와 NO의 외생처리는 고추 묘의 저온 내성을 증대시켰고 병 발생 정도를 감소시키는 데 효과적이었음을 보여준다.

• 혜화의학회지
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• 제9권1호
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• pp.201-214
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• 2000

Precise and detailed clinical research and evaluation based on objective standards are imperative factors in securing reliability of a clinical test. Built on this principle, this clinical test has been conducted during the period between March 1999 and March 2000 dealing with 31 outpatients treated with ShiHo-GuizhiTang or ShiHo-GuizhiTang modify and ShiHo-GuizhiTang mixed prescriptions at the Sung-bo ORIENTAL MEDICAL CLINIC. A variety of information relating to those patients has been collected and analyzed under such criteria as precise diagnoses and their clinical effectiveness. The analysis was duly based on "The theory of cold syndrome". Then, the 31 patients' individual clinical information was compared one another by breaking down the results into sub-categories including gender, age, disease, main symptom, blood pressure, pulse beats, syndrome of abdomen, treatment period, modify and mixed prescription, and evaluation. The result of this clinical test can be summarized as follows: First, ShiHo-GuizhiTang appears to be more frequently prescribed in the case of female patients than in the case of male patients despite the fact that it does not necessarily need to be applied only to female. In addition, the numbers of prescriptions of ShiHo-GuizhiTang in the two age groups consisting of elementary school children and economically active adults respectively were the highest among other age groups. Second, ShiHo-GuizhiTang proved to be most effective in treatment for respiratory ailments and arthritis. Third, ShiHo-GuizhiTang brought down blood pressure of hypertension patients and at the same time benefited patients with normal or lower-than-normal blood pressure who were vulnerable to diseases due to low disease-resistance. Fourth, ShiHo-GuizhiTang was effective in the case of frequent pulse(rapid pulse) and thereby indicating the fact it carries Taiyang superficies syndrome. 지맥 arises from suppressed immune responses owing to adrenocortical hormones. ShiHo-GuizhiTang controls and revitalizes those suppressed immune systems which stem from slow pulse and, as a consequence of that process, helps them return to normal condition of pulse. Fifth, from the standpoint of syndrome of abdomen, feeling of obstruction in the epigastrium serves as an important standard in the process of diagnosing diseases and evaluating effectiveness of treatments. Sixth, according to the results of the clinical test with the 31 patients, a total of 81 percent of test subjects benefited from the treatment. The figure is the sum of 52 percent of I-class (both main symptom and accompanying symptoms had been eliminated) and 29 percent of II-class (part of main symptom and accompanying symptoms had been eliminated) respectively. All told it is safe to say that ShiHo-GuizhiTang can elect to be a viable clinical treatment. In conclusion, it is estimated that this clinical study has drawn up guidelines for objective diagnostic standards and evaluation on specific treatments' effectiveness. This will lead to more general application of ShiHo-GuizhiTang. On top of that, this study could also provide an opportunity to stress the significance of ShiHo-GuizhiTang and ShiHo-GuizhiTang modify and ShiHo-GuizhiTang mixed prescriptions as an alternative treatment for collagen disease which comes from environmental degradation and pollution.

• 한국식품영양과학회지
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• 제38권1호
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• pp.47-51
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• 2009

비타민 C 섭취 및 요가가 중년 여성의 총항산화능, 지질과 산화 및 혈중지질에 미치는 영향을 알아보기 위하여 B광역시 H구에 거주하는 중년여성을 대상으로 12주간 실험을 실시하였다. 요가 프로그램은 주 3회, 1일 60분, 운동 강도 RPE $13{\sim}15$로 실시하였다. 비타민 C는 1일 1 g을 섭취시켰다. 12주 후 총항산화능의 변화는 정상군 28.23%, 비타민 C군 30%, 요가군 26.58%, 비타민 C 섭취와 요가군은 43.66%가 증가하였으나 군간 유의적인 차이가 없었다. 지질과산화물가의 변화는 정상군 10%, 비타민 C군 -25%, 요가군은 -32.14%, 비타민 C 섭취와 요가군은 -33.87%로 정상군에 비해 프로그램군이 유의적으로 감소하였으나 프로그램군간의 유의적인 차이는 없었다. 혈중지질농도 변화는 모든군에서 유의적인 차이가 없어 요가 또는 비타민 C 섭취에 따른 지질개선효과를 관찰할 수 없었다. 이상의 결과를 통해 중년여성에게 비타민 C 섭취나 규칙적인 요가 프로그램은 지질과산화물가를 낮춤으로써 체내 산화적 스트레스를 개선하는 효과가 있는 것으로 생각된다.

• 한국토양비료학회지
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• 제46권1호
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• pp.40-48
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• 2013

양파는 2모작 작물로써 소득이 큰 작물이면서 건강에 좋은 기능성분이 많이 함유하고 있어 생산자와 소비자에게 인기가 높은 식품이다. 최근 건강과 환경을 중시하는 생활패턴 때문에 소비자는 유기농 양파를 더 선호할 것으로 예상되나 자연생태계를 고려하면서 안정적이고 건강한 먹거리를 생산하기는 매우 어렵다. 유기농법의 한계를 극복하고자 양파 잔재물에서 증류법으로 양파 추출액을 채취하여 유기농양파 재배기간 중에 500배로 희석하여 7번 되돌려 주고 생산성과 관련된 생육, 수량, 양분 및 토양미생물을 비교한 결과는 다음과 같았다. 1. 양파의 생육은 대체로 대조구<순환구<관행구 순으로 좋았으며, 초기에는 순환구가 관행구보다 현저히 불량하였으나 후기에 갈수록 처리간 차이가 줄어져 비슷하였다. 2. 양파의 수량은 순환구가 대조구보다 9.3% 증가되었고 관행구보다 11.0% 감소되었으며, 300 g 이상 구중 분포비율이 높을수록 수량도 많아지는 경향이었다. 3. 양파의 무기성분 함량은 순환구가 대조구와 관행구보다 많았으며, 특히 인산, 철, 아연 함량 등은 관행구에 비하여 47%, 78%, 172% 현저히 증가되었다. 4. 양파의 유기성분 함량은 순환구가 대조구와 관행구보다 많았으며, 퀘세틴 함량은 순환구가 대조구와 관행구에 비하여 각각 33.9%, 44.0% 증가되었다. 5. 토양의 내생 균근균은 순환구가 대조구와 관행구에 비하여 각각 40%, 31.7% 더 많았고 미생물 스트레스 지표는 순환구가 대조구와 관행구에 비하여 15%, 20% 감소되었다.

• Journal of Acupuncture Research
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• 제31권4호
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• pp.33-43
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• 2014

This study was performed to investigate the protective effects of acupuncture on the liver in the oxidative stress caused by cadmium accumulation. Sprague-Dawley male($150{\pm}30g$) rats were stabilized for a week and divided into 5 groups which is normal, control, $LR_3$ acupuncture, $BL_{23}$ acupuncture and sham acupuncture group. For three days experimental groups were received oral doses of cadmium 2 mg/kg twice a day. Acupuncture was given bilaterally at each point 10 times for two weeks. The depth of stimulation was 1 mm at right angles and torsion of acupuncture was produced 2 times per second for 1 minutes. The liver was shipped off and taken weight at the last day of two weeks, and hepatic functions was confirmed through alanine transaminase(ALT) and aspartate amino-transferase(AST). We measured reactive oxygen species of serum, liver and kidney, and compared expression levels of superoxide dismutase(SOD), catalase, glutathione peroxidase(Gpx), nuclear factor erythroid derived 2-related factor 2(Nrf-2), heme oxygenase-1(HO-1), nuclear factor-${\kappa}B$ (NF-${\kappa}B$), cyclooxygenase-2(COX-2), inducible nitric oxide synthase(iNOS), Bax and Cytochrome c. $BL_{23}$ acupuncture group significantly increased liver weight and decreased ALT compared to control group. For the oxidative stress, $LR_3$ acupuncture group significantly reduced reactive oxygen species, and $BL_{23}$ acupuncture group significantly reduced reactive oxygen species and inflammation-related protein compared to control group. But $LR_3$ acupuncture group and $BL_{23}$ acupuncture group didn't significantly reduce apoptosis-related protein. Therefore $LR_3$ and $BL_{23}$ acupuncture showed the effects of antioxidant and anti-inflammatory, especially $BL_{23}$ acupuncture was more effective than $LR_3$ acupuncture on the protection of liver in the oxidative stress.

• 한국학교ㆍ지역보건교육학회지
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• 제9권1호
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• pp.33-46
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• 2008

Objectives: The aim of this study was to investigate the correlation between the obesity rate in elementary school students in Daejeon and the relevant factors such as social-demographical factors, genetic factors, birth factors, diet factors and intelligence factors. Methods: For the research, 443 fourth grade students, 405 fifth grade students and 417 sixth grade students from six elementary schools in Daejeon Metropolitan City Participated in this study and classified into three groups: normal-weight group, mild obesity group, and moderate or severe obesity group. Results: The total obesity rate of the elementary school students in the school district of Daejeon Metropolitan City was 14.2%; the rate of mild obesity was 7.2%; the rate of moderate obesity was 5.5%; and the rate of severe obesity was 1.5%. The obesity rate of surveyed boys was 16.9%, and the obesity rate of girls was 11.1%. The significant factors for girls' obesity were fathers' EMI, mothers' EMI, living standards, constant demand of foods, preference for greasy foods, frequency of eating snacks, and daily walking hours. It was found that students' subjective mind and objective health index were related to obesity. In the case of obese students, they had more concern about their health and more stress from the dissatisfaction of their physical appearance than normal students. Conclusions: From the above evidences, it is apparent that the obesity of elementary school students has strong relations with eating habits rather than physical activities. It is to be hoped that obesity prevention programs such as effective meal guidance. parental guidance for watching TV, and intense physical activities will be included in the curriculums of health education for elementary school students.

• 대한본초학회지
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• 제31권2호
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• pp.7-12
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• 2016

Objectives : Secondary amenorrhoea is the absence of menses for three months in a woman with previously normal menstruation or nine months for women with a history of oligomenorrhoea. It can be caused by stress, extreme weight loss, and excessive exercise. The purpose of this study was to report the clinical effects of herbal medicine on secondary amenorrhoea.Methods : We employed oriental medical treatments; herbal - medication (Hyunburikyungtang gamibang), acupuncture and moxibustion. We treated the patients one or two times a month with oriental therapy method. They took medicine three times a day after a meal. During taking medicine, we let the patients avoid fatty food, flour based food and alcohol. We evaluated the status of the patient, on the basis of the state of menstration and F2 level of Yangdorak. Because we diagnosed the condition of patients with the pattern of liver depression and qi stagnation, so F2 level of Yangdorak was an important point of the diagnosis. Yangdorak machine was Tormeter Iw - zen at Towatech Co.,Ltd.Results : After taking treatment - several times acupuncture and moxibustion during some period and taking herbal-medicine, they had menstrain naturally without taking any hormone drug. Also the amount of menstration has gradually increased. The F2 level of Yangdorak returned to normal range. The feeling of cold on hands, feet and lower abdomen was much improved.Conclusions : Herbal medicine (Hyunburikyungtang gamibang) with oriental medical treatments, acupuncture and moxibustion was effective in the treatment of secondary amenorrhoea.

• Nutrition Research and Practice
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• 제9권1호
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• pp.37-42
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• 2015

BACKGROUND/OBJECTIVES: Red grape seeds as functional food are a good source of important bioactive components such as phenolics and antioxidants, which decrease oxidative stress that contributes to the pathogenesis of hepatotoxicity. The current study was conducted in order to evaluate the protective effect of red grape dried seeds (RGDS) on antioxidant properties, lipid metabolism, and liver and kidney functions of rats with paracetamol (750 mg/kg) induced hepatotoxicity. MATERIALS/METHODS: RGDS was added to the basal diet at 5, 10, and 20%. Thirty five adult male rats were assigned to five groups (n = 7) for a six-week feeding period; group (1) normal control, group (2) induced control, groups (3, 4, and 5) fed a diet with RGPS at different levels, 5, 10, and 20%, respectively. At the end of the feeding period, animals' blood and tissues were collected for estimation of serum lipid profile, serum liver, and kidney biomarkers. The protection was measured by detecting lipid peroxidation (LPO), glutathione (GSH), superoxide dismutase (SOD), Catalase (CAT) (in liver tissues), and liver histological examination. RESULTS: The results showed a significant (P < 0.05) decrease in levels of serum cholesterol, triglycerides, low density lipoprotein (LDL-C), and very low density lipoprotein (VLDL-C), with a significant increase in level of high density lipoprotein (HDL-C) for RGDS groups compared to induced control. Rats administered a diet containing RGDS levels produced significant (P < 0.05) hepatoprotection by decreasing the activities of liver enzymes, kidney parameters, and lipid peroxidation, while levels of GSH, SOD, and CAT were increased significantly to near the normal levels. CONCLUSION: The RGDS 20% group was more effective than others against hepatotoxicity of paracetamol, which may be attributed to RGDS total phenols and antioxidant contents, which were 1.438 mg and 1.231 mg, respectively.

• 한국철도학회논문집
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• 제12권1호
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• pp.16-24
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• 2009

선형탄성파괴역학을 적용하여 균열이 발생한 변동하중하의 철도차량 대차틀에 대한 균열성장속도를 예측하였다. 이를 위하여 철도차량 대차틀의 균열발생사례를 분석하여 취약부위를 파악하였으며, 영업노선에서의 실동하중 측정과 구조해석을 통한 정하중 계산으로 대차틀 취약부에서 운행 중 받는 총 하중이력을 생성하였다. 총 하중이력에서 균열닫힘을 고려한 유효하중이력을 계산하였으며, 취약부 3곳에서 균열성장속도를 예측하고 일본에서 측정한 균열진전 사례와 비교하였다. 해석결과 초기길이 40mm의 균열이 급속한 균열성장을 일으키기까지는 약 50만km의 주행거리가 필요하며 이는 약 3.8년의 운행기간에 해당하므로 도시철도의 유지보수기간을 고려하면 임계균열로 도달하기 전에 충분히 감지할 수 있을 것으로 생각된다.

• 대한간호학회지
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• 제49권2호
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• pp.149-160
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• 2019

Purpose: The purpose of this study was to examine the effects of oral cryotherapy on oral mucositis, reactive oxygen series, inflammatory cytokines, and oral comfort in patients undergoing chemotherapy for gynecologic cancers. Methods: Participants were randomly assigned to the experimental group (n=25, receiving oral cryotherapy during chemotherapy) and the control group (n=25, receiving the usual care consisting of 0.9% normal saline gargles three times before meals). Oral mucositis was assessed using the oral assessment guide, while oral comfort was assessed using the oral perception guide. Reactive oxygen series was measured as total oxidant stress, and the level of two inflammatory markers, interleukin-6 (IL-6) and tumor necrosis factor-alpha ($TNF-{\alpha}$), were examined. The data were analyzed using t-test, chi-square test, Fisher's exact test, Mann-Whitney U-test, and repeated measures analysis of variance. Results: There was a significant difference in the oral mucositis score, reactive oxygen series score, $TNF-{\alpha}$ level, and oral comfort score between the two groups, and there were significant changes over time and in the group-by-time interactions. There was a significant difference in the IL-6 score between the two groups, but there were no significant changes over time or in the group-by-time interactions. Conclusion: The study results revealed that oral cryotherapy was more effective than the usual care regime of normal saline gargles for reducing oral mucositis, reactive oxygen series, and inflammatory cytokines and for improving oral comfort in gynecologic cancer patients undergoing chemotherapy.