• Nutrition Research and Practice
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• 제14권3호
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• pp.203-217
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• 2020

BACKGROUND/OBJECTIVE: Centella asiatica, also known as Gotu kola, is a tropical medicinal plant native to Madagascar, Southeast Asia, and South Africa. It is well known to have biological activities, including wound healing, anti-inflammatory, antidiabetic, cytotoxic, and antioxidant effects. The purpose of this study was to determine the efficacy of extracts of C. asiatica against age-related eye degeneration and to examine their physiological activities. MATERIALS/METHODS: To determine the effects of CA-HE50 (C. asiatica 50% EtOH extract) on retinal pigment cells, we assessed the cytotoxicity of CoCl₂ and oxidized-A2E in ARPE-19 cells and observed the protective effects of CA-HE50 against N-methyl-N-nitrosourea (MNU)-induced retinal damage in C57BL/6 mice. In particular, we measured factors related to apoptosis and anti-oxidation and the protein levels of rhodopsin/opsin. We also measured glucose uptake to characterize glucose metabolism, a major factor in cell protection. RESULTS: Induction of cytotoxicity with CoCl₂ and oxidized-A2E inhibited decreases in the viability of ARPE-19 cells when CA-HE50 was administered, and promoted glucose uptake under normal conditions (P < 0.05). In addition, CA-HE50 inhibited degeneration/apoptosis of the retina in the context of MNU-induced toxicity (P < 0.05). In particular, CA-HE50 at 200 mg/kg inhibited the cleavage of pro-caspase-3 and pro-poly (ADP-ribose)-polymerase and maintained the expressions of nuclear factor erythroid 2-related factor 2 and heme oxygenase-1 similar to normal control levels. Rhodopsin/opsin expression was maintained at a higher level than in normal controls. CONCLUSION: A series of experiments confirmed that CA-HE50 was effective for inhibiting or preventing age-related eye damage/degeneration. Based on these results, we believe it is worthwhile to develop drugs or functional foods related to age-related eye degeneration using CA-HE50.

• 대한치과의사협회지
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• 제54권3호
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• pp.206-216
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• 2016

Objectives The aim of this study was to evaluate the antibacterial effect of xanthorrhizol (XTZ) on E. faecalis, compared with 2% chlorhexidine (CHX). Materials and Methods Normal physiological state (NS), starvation state (SS), and alkalization state (AS) of E. faecalis were used. A solution containing 1% XTZ in 30% ethanol, 1% dimethyl sulfoxide (DMSO), and 100 mg/ml sodium methyl cocoyl taurate was used and is referred to as Xan in this study. To determine the minimal bactericidal concentration (MBC) of Xan and CHX, $500{\mu}l$ of E. faecalis (NS and two stress states) was added to a microtube containing $500{\mu}l$ of serial 2-fold dilutions of 1% Xan and 2% CHX (1:2-1:128). The MBC of each antimicrobial was determined by the plate count method. Results The antibacterial effect of Xan was more effective on E. faecalis in AS than in the other states (NS, SS) at 0.125% Xan and 0.03325% Xan (P<0.05). In contrast, the antibacterial effect of CHX was more effective against E. faecalis in SS than the other states (NS, AS) at 0.0625% CHX (P<0.05). In SS, the antibacterial effect of CHX was more effective than that of Xan at 0.125% and 0.0625% (P<0.05). However, in AS, the antibacterial effect of Xan was more effective than that of CHX at 0.0625% and 0.03325% (P<0.05). Conclusions In endodontic retreatment cases in which it is important to effectively remove E. faecalis from the infected root canal, Xan may be more suitable when combined with NaOCl than CHX.

• The Journal of Korean Physical Therapy
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• 제22권6호
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• pp.65-70
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• 2010

Purpose: Exercise has been shown to be a simple and economical therapeutic modality that may be considered as an effective aid for diabetic mellitus. For example, exercise training increases insulin sensitivity in type 2 diabetes. But we found no reported of how exercise affect type 1 diabetes. This study investigated the impact of aerobic and graduated treadmill exercise regimens on body weight, glucose and insulin concentrations, lipid profiles, and oxidative stress indicators in rats with streptozotocin (STZ) induced diabetes. Glycosylated hemoglobin ($HbA_{1c}$) was determined as an indicator of glucose control during exercise. Methods: In our study, a total of 40 rats were used. Three groups of 10 rats each were given STZ to induce diabetes. The remaining 10 rats became the normal group. After 28 days we determined biochemical parameters such as glucose, glycosylated hemoglobin ($HbA_{1c}$), insulin concentration, serum total cholesterol (TC), triglycerides (TG), and high-density lipoprotein (HDL). Superoxide dismutase (SOD) and catalase activities were also measured. Results: Concentrations of blood glucose and $HbA_{1c}$ in the moderated exercise groups were significantly decreased after 28 days compared with the control group (p<0.05). There was a significant reduction in serum TC and TG in the experimental groups. The activity of SOD increased significantly by 17.70% and 48.25% respectively. Conclusion: These results indicate that physical training and exercise training affects body weight, fasting blood glucose, $HbA_{1c}$, insulin, lipid profiles, and antioxidant status in rats with streptozotocin-induced diabetes. We suggest that graduated treadmill exercise may have therapeutic, preventative, and protective effects against diabetes mellitusby improving glycemic control, oxidant defenses, and lipid metabolism.

• Preventive Nutrition and Food Science
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• 제19권1호
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• pp.19-26
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• 2014

Increased consumption of fresh vegetables that are high in polyphenols has been associated with a reduced risk of oxidative stress-induced disease. The present study aimed to evaluate the antioxidant effects of spinach in vitro and in vivo in hyperlipidemic rats. For measurement of in vitro antioxidant activity, spinach was subjected to hot water extraction (WE) or ethanol extraction (EE) and examined for total polyphenol content (TPC), oxygen radical absorbance capacity (ORAC), cellular antioxidant activity (CAA), and antigenotoxic activity. The in vivo antioxidant activity of spinach was assessed using blood and liver lipid profiles and antioxidant status in rats fed a high fat-cholesterol diet (HFCD) for 6 weeks. The TPC of WE and EE were shown as $1.5{\pm}0.0$ and $0.5{\pm}0.0mg$ GAE/g, respectively. Increasing the concentration of the extracts resulted in increased ORAC value, CAA, and antigenotoxic activity for all extracts tested. HFCD-fed rats displayed hyperlipidemia and increased oxidative stress, as indicated by a significant rise in blood and liver lipid profiles, an increase in plasma conjugated diene concentration, an increase in liver thiobarbituric acid reactive substances (TBARS) level, and a significant decrease in manganese superoxide dismutase (Mn-SOD) activity compared with rats fed normal diet. However, administration of 5% spinach showed a beneficial effect in HFCD rats, as indicated by decreased liver TBARS level and DNA damage in leukocyte and increased plasma conjugated dienes and Mn-SOD activity. Thus, the antioxidant activity of spinach may be an effective way to ameliorate high fat and cholesterol diet-induced oxidative stress.

• 콘크리트학회논문집
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• 제16권5호
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• pp.621-626
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• 2004

일반 콘크리트뿐만 아니라 고성능콘크리트 제조 시 고로슬래그(BFS)의 사용은 워커빌리티, 장기 강도 및 내구성 측면에서 장점을 갖는다. 그러나 슬래그 콘크리트는 일반 콘크리트에 비해 수축이 크며 특히 자기수축이 크게 발생하기 때문에 적절한 방법으로 제어하지 않으면 심각한 균열을 야기할 수 있다. 따라서 수축에 의한 균열 발생을 최소화하고 콘크리트 구조물의 사용 수명을 확보하기 위해서는 BFS를 함유한 콘크리트의 자기수축 거동에 대한 이해가 요구된다. 본 연구에서는 물-결합재(시멘트+BFS) 비(W/B)가 0.27${\~}$0.42이고 BFS 대체율이 각각 $0\%$, $30\%$, $50\%$인 각주형 콘크리트 시편을 제작하여 자기수축을 측정한 후, 실험결과를 바탕으로 자기수축 예측 모델의 재료 상수 값들을 결정하였다. 또한, 응력 발현에 기여하는 자기수축을 유효자기수축으로 정의하고, 다양한 W/B를 고려한 재령 28일에서의 유효자기수축 변형률 추정식을 제안하였다. 실험결과, W/B가 동일할 때 콘크리트의 자기수축은 BFS의 사용량에 따라 증가하였다. 또한 동일한 양의 BFS를 사용한 경우, W/B가 낮아짐에 따라 자기수축 증가율이 감소하는 경향을 보였다. 따라서 고로슬래그 콘크리트의 자기수축을 줄이기 위해서는 자기수축을 줄이는 수축저감제 등의 혼화 재료를 사용하거나 시공 현장에서의 충분한 습윤양생이 필요하다고 판단된다.

• 동의생리병리학회지
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• 제27권5호
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• pp.611-616
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• 2013

Alcoholic liver disease (ALD) is a major cause of morbidity and mortality around the world. Although much progress has been made in understanding the pathogenesis of ALD, there remains no effective therapy for it. Accumulated evidence indicates that oxidative stress is the main pathological factors in the development of ALD. Ethanol administration causes accumulation of reactive oxygen species (ROS), including superoxide, hydroxyl radical, and hydrogen peroxide. ROS, in turn, cause lipid peroxidation of cellular membranes, and protein and DNA oxidation, which results in hepatocyte injury. In addition to pro-oxidants formation, antioxidants depletion caused by ethanol administration also results in oxidative stress. The objective of this study is to investigate the effects of Shiryung-tang extract on the chronic alcoholic liver injury induced by EtOH. Male Sprague Dawley rats were used in this study. All rats were maintained under standard laboratory conditions ($23{\pm}1^{\circ}C$, 12h light/12h dark cycles). All animals (n=30) were randomly divided into following groups: (1) Normal group, treated with distilled water (n=10); (2) Control group, treated with ethanol (n=10); (3) Sample group, treated with ethanol + pharmacopuncture (n=10). For oral administration of ethanol in Control and Sample group, the ethanol was dissolved in distilled water in concentrations of 25%(v/v). Throughout the experiment of 8 week, the rats were allowed free access to water and standard chow. Sample group were administrated by Shiryung-tang extract daily for 8 weeks. Control group were given normal saline for same weeks. As a results, the oral administration of ethanol for 8 weeks leads to hepatotoxicity. The levels of hepatic marker such as HDL-cholesterol, triglyceride, aspartate aminotransferase and alanine aminotransferase were altered. The ethanol also increased lipid peroxidation and depletion of antioxidant enzyme activities as well as hepatic tissue injury. However, the treatment of Shiryung-tang extract prevented all the alterations induced by ethanol and returned their levels to near normal. These data suggest that Shiryung-tang extract could have a beneficial effect in inhibiting the oxidative damage induced by chronic ethanol administration. Therefore, Shiryung-tang extract can be a candidate to protect against EtOH-induced liver injury.

• Nutrition Research and Practice
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• 제4권4호
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• pp.295-302
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• 2010

The aims of this study were to evaluate obesity-related dietary behaviors and to determine long-term exercise effects on obesity and blood lipid profiles in elderly Korean subjects. A total of 120 subjects, aged 60-75 yr, were recruited, and obesity-related dietary behaviors were determined. An exercise intervention was conducted with 35 qualified elderly females for 6 months, and body composition and blood lipids were measured 6 times at 4 week intervals. At baseline, mean BMI ($kg/m^2$) was 24.8 for males and 23.1 for females. The females had better eating habits than the males and were more concerned with reading nutrition labels on food products (P < 0.001); they also preferred convenience foods less than the male subjects (P < 0.05). Obese individuals were more likely than overweight or normal weight individuals to misperceive their weight (P < 0.001). Those with a high BMI responded feeling more depressed (P < 0.01), lacking self-confidence (P < 0.01), and feeling isolated (P < 0.01) as well as having more difficulty doing outdoor activities (P < 0.01). After exercise, body fat (%) and WHR were significantly reduced (P < 0.05), while body weight and BMI were also decreased without statistical significance. Total cholesterol and blood HDL were significantly improved (207.1 mg/dl vs. 182.6 mg/dl, HDL: 45.6 mg/dl vs. 50.6 mg/dl, P < 0.05). Other benefits obtained from exercise were improvements in self-confidence (26.4%), movement (22.6%), stress-relief (18.9%), and depression (13.2%). In conclusion, elderly females had better eating habits and were more concerned with nutrition information and healthy diets compared to elderly males. However, misperceptions of weight and obesity-related stress tended to be very high in females who were overweight and obese, which can be a barrier to maintain normal weight. Long-term Danhak practice, a traditional Korean exercise, was effective at reducing body fat (%) and abdominal obesity, and improved lipid profiles, self-confidence, and stress.

• Fisheries and Aquatic Sciences
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• 제23권1호
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• pp.1.1-1.14
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• 2020

Background: Dietary protein requirements are dependent on a variety of factors and water temperature is one of the most important abiotic factors affecting protein requirement of fish. This study was, therefore, conducted to investigate effects of water temperature on dietary protein requirement of fry Heteropneustes fossilis which has high demand in most of the Asian markets. Methods: Quadruplicate groups of 30 fish per treatment (2.97 ± 0.65 cm; 5.11 ± 0.34 g) were fed seven isoenergetic diets (17.9 kJ g^-1 gross energy; 14.99 kJ g^-1 digestible energy) containing dietary protein levels ranging from 28 to 52% at two water temperatures (18 and 26 ℃). Experimental diets were fed to apparent satiation as semi-moist cakes thrice daily at 17:00, 12:00, and 17:30 h for 12 weeks. For precise information, various growth parameters, protein deposition, hematological parameters, metabolic enzymes, and stress response were analyzed, and effects of water temperature on dietary protein requirement was recommended on the basis of response from above parameters. Results: Groups held at 26 ℃ attained best growth, feed conversion, and protein deposition at 44% dietary protein indicating that temperature affected dietary protein requirement for optimum growth of H. fossilis fry and protein requirement seems to be satisfied with 44% dietary protein. Interestingly, interactive effects of both dietary protein levels and temperature were not found (P > 0.05). Fish reared at 18 ℃ had comparatively higher values for aspartate and alanine transferases than those reared at 26 ℃ water temperature which exhibited normal physiological value for these enzymes indicating that body metabolism was normal at this temperature. Hematological parameters also followed same pattern. Furthermore, fish reared at 26 ℃ water temperature exhibited more resistant to thermal stress (P < 0.05). The 95% maximum plateau of protein deposition data using second-degree polynomial regression analyses exhibited dietary protein requirement of fry H. fossilis between 40.8 and 41.8% of diet at 26 ℃ water temperature. The recommended range of dietary protein level and protein/digestible energy ratio for fry H. fossilis is 40.8-41.8% and 27.21-27.88 mg protein kJ^-1 digestible energy, respectively. Conclusions: Information developed is of high significance for optimizing growth potential by making better utilization of nutrient at 26 ℃ and, to develop effective management strategies for mass culture of this highly preferred fish species.

• 한국보건간호학회지
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• 제14권2호
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• pp.229-245
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• 2000

Previous studies have shown that five representative behaviors affect the health of adults in everyday life : smoking, drinking, exercise, nutrition, and stress. This study focuses on these five behaviors in an attempt not only to develop a program that promotes health, but also to suggest ways that such a program may be implemented to reflect diverse lifestyles. Our aim is to show how individual subjectivity influences behavior when making lifestyle choices that affect health. By analyzing individual characteristics, we tried to group people according to their common attitude and behaviors to promote health. We hope that this study may provide the fundamental data which may be used to assist professionals in promoting healthy behaviors among adults. In order to examine how subjectivity(e.g, personal opinions or attitudes) influences behavior, we prepared Q-statements which were composed of Q-samples for the study of human subjectivity. We polled forty-two adults and then analyzed the results using a PC qunal program. As a result, the respondents were classed according to six different types. Type One includes people who think managing stress is the most effective way to live a healthy life. They regard smoking and drinking as harmful behaviors. Type Two subjects, on the other hand, regard smoking and drinking as a matter of taste with few harmful effects. Although they recognize the importance of managing stress, they have a positive attitude towards smoking and regard nutrition as a minor factor in promoting their healthy lifestyle. Those classed as Type Three emphasize the necessity of exercise. They perceive drinking, smoking, and stress as harmful. People in this category seek to increase physical strength and to regulate all five health behaviors by keeping them in a normal range. Type Four consists of people who are indifferent to their health. While they seem to recognize the importance of regular exercise, they pay little attention to nutrition or to the harmful effects of smoking, drinking, and stress. They believe that exercise alone is sufficient to maintain their health. Type Five subjects believe drinking is not a harmful behavior at all. Rather. it has a positive effect on their mental health. They are characterized by this positive attitude towards drinking as well as by indifference toward nutrition (although they readily admit that good nutrition is important). Finally. those classed as Type Six have a positive attitude toward moderate drinking as a means to relieve stress. However. they also recognize the harmful effects of excessive drinking. They regard good nutrition as an important lifestyle choice but are indifferent toward exercise. This type is engaged in passive health management. This study devised six types or categories that reflect different attitudes toward promoting health in everyday life. It further went on to analyze the characteristics of each type. This study shows that programs designed to promote health must be modified to reflect the diversity of individual attitudes and patterns of behaviors.

• 한국지반공학회논문집
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• 제24권10호
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• pp.25-32
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• 2008

암반에 근입된 현장타설말뚝의 주면마찰력은 암석의 일축압축강도 외에 굴착면의 거칠기, 암반의 재료특성, 초기 수직응력, 말뚝의 직경, 암반의 절리 및 풍화도 등에 크게 영향을 받는 것으로 알려져 있다. 특히 주면마찰력에 영향을 미치는 인자 중 굴착공의 거칠기는 암반의 종류, 말뚝의 직경, 그리고 말뚝의 시공법에 따른 영향이 큰 것으로 알려져 있다. 암반의 굴착공 벽면거칠기 측정장치인 Backyoung-Kyungsung Laser Roughness Profiling System(이하 BKS-LRPS라 칭함)의 혼탁도에 대한유효측정영역 및 측정 가능 여부를 결정하기 위하여 실내모형실험을 수행하였으며, 여기서 혼탁($T_b$)와 BKS-LRPS의 유효 측정거리(Effective Measurement Distances(EMD), mm)의 관계식은 $EMD=1149.2{\times}T_{b}^{-0.64}$로 구할 수 있었다.