• Research in Plant Disease
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• v.27 no.3
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• pp.99-106
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• 2021

The fungal isolate Isaria javanica pf185 has potential as a mycopesticide because it demonstrates insecticidal activity against the green peach aphid and antifungal activity against Colletotrichum gloeosporioides. For commercialization of this isolate, determination of the optimal and least expensive culture conditions is required; however, these data are not currently available. This study describes the conditions for optimal development of conidia and production of metabolites for the biocontrol of the fungal pathogen. The optimal culture conditions were examined using cultures on solid agar and liquid media. High growth temperature enhanced spore formation but reduced antifungal activity in both solid and liquid media. The highest spore yield was obtained in a medium containing glucose as a carbon source and yeast extract as a nitrogen source. Soybean powder and wheat bran were effective nitrogen sources that promoted spore production and antifungal activity of the isolate. These results revealed the basic, cost-effective growth media for commercial production of a biopesticide with insecticidal and antifungal properties for use in integrated pest management.

• Biotechnology and Bioprocess Engineering:BBE
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• v.9 no.6
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• pp.459-464
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• 2004

The objective of this study is to improve cephalosporin C (CPC) production byoptimization of medium and culture conditions. A statistical method was introduced to optimize the main culture medium. The main medium for CPC production was optimized using a statistical method. Glucose and corn steep liquor (CSL) were found to be the most effective factors for CPC production. Glucose and CSL were optimized to 2.84 and $6.68\%$, respectively. CPC produc­tion was improved $50\%$ by feeding of $5\%$ rice oil at day 3rd and 5th day during the shake flask culture of C acremonium M25. The effect of agitation speeds on CPC production in a 2.5-L bio­reactor was also investigated with fed-batch mode. The maximum cell mass (54.5 g/L) was obtained at 600 rpm. However, the maximum CPC production (0.98 g/L) was obtained at 500 rpm. At this condition, the maximum CPC production was improved about $132\%$ compared to the re­sult with batch flask culture.

• Microbiology and Biotechnology Letters
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• v.17 no.5
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• pp.519-523
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• 1989

The effects of carbon, nitrogen sources and culture conditions on glucoamylase production from Aspergillus sp. were investigated. Among tested carbon sources, soluble starch was most effective for the production of the enzyme, and the level of concentration for the optimal enzyme production was found to be 5%. For nitrogen sources, yeast extract was best for the enzyme production, with the level of 0.1%. The enzyme was maximally produced by cultivating the organism at medium of initial pH 6.0, and temperature of 28$^{\circ}C$. Wheat bran was most suitable for the enzyme production from the organism in solid state culture.

• Journal of Fisheries and Marine Sciences Education
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• v.27 no.1
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• pp.308-318
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• 2015

In order to improve the competitiveness of Korean large purse seine fishery, the purpose of this study is to find out the problems and present the improvement of the production structure of Korean large purse seine fishery by comparing to the production structure of Norwegian purse seine fishery. Norwegian purse seine fishery has been operating labor and energy-saving systems through a single wire operation and avoiding fishing competition through the introduction of IVQ system with different types of fishing vessel and obtaining benefits from Leading fishery management integrated with fish pumps and cooling water systems. In contrast, our country has a large purse seine fishery issues such as high cost and low efficiency of production structures, the volatility of mackerel resources, the lack of leading fishery management after catching fishes, the reduction of fishing ground in accordance with the North East Asia EEZ system. To solve these problems, initiatives to improve the structure of our large purse seine fishery are as follows: First, Implementing the promotion of the effective immature fishes management and establishing Korea-China-Japan resource management system. Second, the promotion of reduction of fleet size, the review of possible single fishing operation, leading out to strengthen its competitiveness by switching to energy-efficient production systems through enhanced efforts at fisheries catches steps. Third, it is necessary to realize corporate through the diversification of business areas and the large-scale commercialization of production structure.

• Korean Journal of Medicinal Crop Science
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• v.12 no.5
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• pp.378-383
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• 2004

Scopolamine and hyoscyamine are important anticholinergic compounds. To increase the productivity, we have selected various elicitors and developed culture system using a bubble column bioreactor (BCB). As the same manner of elicitation in flask cultures, the elicitors were introduced into BCB cultures and the productivity was investigated. Except the bacterial elicitor of Staphyllococcus aureus, the elicitors inhibited hyoscyamine production. In scopolamine production, the elicitors revealed different responses from the results obtained in flask cultures. The elicitors of KCl and Candida albicans less increased the production than flask cultures. However, methyl jasmonate and S. aureus showed stronger positive effects on tropane alkaloid production. In particular, S. aureus was the most effective elicitor on scopolamine production and the elicitor resulted in the highly increased production, approximately 10 times higher than the control culture.

• The Journal of Information Systems
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• v.26 no.2
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• pp.143-164
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• 2017

Purpose The purpose of this study is to investigate to investigate the effects of cloud system on the efficiency improvement and cost-saving of production processes. It also tries to provide small- and medium-sized companies and IT practitioners with a practical guideline for a successful implementation of could systems. Design/methodology/approach This study was conducted by observing and analyzing a case of implementing a cloud system at a small-sized company having multiple job sites in terms of the improvement in data-sharing efficiency and cost-saving of production processes. Findings This study found that cloud system was an effective way of sharing data between and among production processes of geographically scattered job sites and thus, helped the company to remedy problems of work schedule and load-balance along sequential production processes. Cloud system also allowed the company to reduce the number of visits made by 3-inspectors to four job sites by 75% and the personnel cost related to inspectors' site visit.

• Asian-Australasian Journal of Animal Sciences
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• v.17 no.5
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• pp.684-687
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• 2004

A study was conducted to determine the effect of live weight of pheasants on egg production and egg quality characteristics. A total of 48 ring-necked pheasants were divided into control, heavy, middle and light BW groups. Live weights of the control were 1,187 g, 1,352.92 g for heavy group, 1,247.92 g for middle group and 1,003.33 g for light group. Egg production of groups were found as 47.32, 42.82, 45.79 and 46.51% respectively, in 10 weeks of period. There were no statistical differences on egg production among the groups. The effect of live weight on egg weight, shape index, specific gravity, shell weight, shell thickness, Haugh Unit, yolk weight and albumen weight were found statistically significant (p<0.05). The effect of live weight on albumen index, membrane weight and membrane thickness were found not important (p>0.05). On the other hand, other important factors to be effective on the egg quality of pheasants should be investigated. As a result of this study, live weight of pheasant hens is not an important factor to obtain high egg production in pheasants. But, body conformation of breeding materials should be in good conditions.

• Asian-Australasian Journal of Animal Sciences
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• v.19 no.8
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• pp.1174-1178
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• 2006

An experiment was conducted to investigate the effects of dietary supplementation of copper chelates in the form of methionine, chitosan and yeast on the performance of laying hens. Four hundred ISA Brown layers, 84 wks old, were assigned to 4 treatments: control, 100 ppm Cu in methionine chelate (Met-Cu), 100 ppm Cu as chitosan chelate (Chitosan-Cu) and 100 ppm Cu as yeast chelate (Yeast-Cu). Each treatment had five replicates of 20 hens. Hen-day and hen-housed egg production and egg weight were significantly (p<0.05) increased by Met-Cu supplementation. The increase by Chitosan-Cu and Yeast-Cu supplementation was not significant. Contrast of the control vs. Cu chelates showed egg weight was significantly (p<0.05) increased by Cu chelate supplementation. Soft-shell egg production was significantly (p<0.05) reduced by supplementation of Cu chelates. Met-Cu treatment showed the lowest incidence of soft egg production. Gizzard erosion index was increased by Cu chelate supplementation. Crude fat in liver, total cholesterol in yolk and Cu content in liver and yolk were not significantly influenced by Cu chelate supplementation. It was concluded that dietary supplementation of 100 ppm Cu as Met-Cu significantly increased egg production and egg weight. Cu-Met chelate was also effective in reducing soft-shell egg production but increased gizzard erosion index.

• Journal of Microbiology and Biotechnology
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• v.19 no.11
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• pp.1385-1392
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• 2009

This work evaluates the effect of different amino acids on production of Cyclosporin (CyA) production in solid-state fermentation that was previously optimized for different fermentation parameters by one factor at-a-time for the maximum production of CyA by Tolypocladium inflatum MTCC557. Based on the Plackett-Burman design, glycerol, ammonium sulfate, $FeCl_3$, and inoculum size were selected for further optimization by response surface methodology (RSM). After identifying effective nutrients, RSM was used to develop mathematical model equations, study responses, and establish the optimum concentrations of the key nutrients for higher CyA production. It was observed that supplementation of medium containing (% w/w) glycerol, 1.53; ammonium sulfate, 0.95; $FeCl_3$, 0.18; and inoculum size 6.4 ml/5g yielded a maximum of 7,106 mg/kg as compared with 6,480 mg CyA/kg substrate using one factor at-a-time. In the second step, the effect of amino acids on the production of CyA was studied. Addition of $_L$-valine and $_L$-leucine in combination after 20 h of fermentation resulted in maximum production of 8,166 mg/kg.

• Microbiology and Biotechnology Letters
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• v.27 no.2
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• pp.91-95
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• 1999

To investigate the effect of salts on the production of erythritol by Torula sp., cells were grown on the media containing various concentrations of KCl or NaCl. Cell growth and glucose consumption rates decreased when KCl or NaCl concentration increased from 0.0 to 0.5M. The production of erythritol, however, was maximal at 0.3M aCl. The erythritol concentration of 54.3g/l in the medium containing 0.3M NaCl and 200g/l glucose was obtained after 120h. The production of erythritol decreased in cultures above 0.3M NaCl or 0.4M KCl due to the inhibition of cell growth. To elucidate the effect of salts more quantitatively, KCl and NaCl concentrations were converted to osmotic pressure. As the osmotic pressure increased, the yield of erythritol from glucose increased regardless of the kinds of salts and the yield of erythritol was approximately 49% at the osmolality of 2.4Os/kg. When the osmotic pressure increased to 2.5Os/kg, the specific growth rate of cells decreased but the production rate of erythritol increased. For the effective production of erythritol, osmotic pressure must be adjusted not to inhibit markedly the growth rate of cells and to stimulate the production rate of erythritol by supplementing salt.