Vinclozolin(VCZ), a systemic dicarboximide fungicide, has been used in the control of diseases caused by microorganism of some species in fruits, vegatables and ornamental plants. Although VCZ itself is a very weak antagonist for androgen receptor binding, both melabolites M1 and M2 are effective antagonists. The present study was undertaken to examine whether prepubertal exposure to VCZ affects on the onset of puberty and the associated reproductive parameters such as hormone receptor expressions in female rats. VCZ(10 mg/kg/day) was administered daily from postnatal day 21(PND 21) through the day when the first vaginal opening(V.O.) was observed. Gross anatomy and weight of reproductive tissues were compared to test the VCZ's effects on the cell proliferation. Furthermore, histological studies were performed to assess the structural alterations in the tissues. To determine the transcriptional changes in progesterone receptor(PR), total RNAs were extracted and applied to the semi-quantitative reverse transcription polymerase chain reaction(RT-PCR). As a result, delayed V.O. was shown in the VCZ group(PND $34.00{\pm}1.22$) compared to the control group(PND $38.20{\pm}1.92$; p<0.01). VCZ treatment significantly decreased the wet weight of ovaries and uteri compared to the control group(p<0.01). Graafian follicles and corpora lutea were observed only in the ovaries from the control animals, while numerous primary, secondary follicles and small atretic follicles were observed in the ovaries from VCZ group. Similarly, hypotrophy of luminal and glandular uterine epithelium was found in the VCZ group. In the semi-quantitative RT-PCR studies, the transcriptional activity of PR in ovary(p<0.01) from VCZ group were significantly lower than those from the control group while in uterus were similar compared with the control group. The present studies demonstrated that the acute exposure to VCZ during the critical period of prepubertal stage could inactivate the reproductive system resulting delayed puberty in female rats.
Journal of The Korean Society of Grassland and Forage Science
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v.5
no.1
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pp.62-72
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1985
In order to find out the effects of seeding time on growth, dry matter production and nutritive content of Pioneer 931, Pioneer 988 and Piper, this study was carried out on the experimental field of Livestock Experiment Station in 1981-83. Seeding time were 7 with 14-day interval from April 16 to July 9. The results are summarized as follows: 1. It tool about 12 to 13 days from seeding to emergence in case of Mid-April seeding and 7 to 8 days in Late-June. Earlier seeding, more longer growth period from emergence to heading they required. 2. Plant height of Pioneer 931 seeded lately was longer than 4.5 meters in primary growth and Sudangrass was about 2.0 to 2.5 meters. Leaf area was the greatest in Mid-August by early seeding but it was increased until Early-October by late seeding. 3. Sorghum gas brought the highest yield in dry mater and Sudangrass the lowest. In general dry matter yield reduced gradually in response to later seeding but Pioneer 931 has brought more than 10 tons per hecter until Late-June. 4. Relative Growth Rate, Leaf Area Ratio and Leaf Weight of all varieties decreased in accordance with growth development. 5. Crude protein content of leaf was higher than stem and the younger the plants, the more protein they contain. Nitrogen Free Extract was just opposite to crude protein.
BACKGROUND: The scab, which is caused by Venturia nashicola, gives serious damages to pear trees. 'Niitaka' accounts for 82% of areas in pear cultivation. However 'Niitaka' is a scab susceptible cultivar. So, most of Korean farmers who growing pear trees have suffered by economic losses with the scab. In this research, we evaluated the scab resistance among elite pear seedlings to clarify genetics about the scab resistance. And we analyzed photosynthetic features with these seedlings to develop suitable cultivar which is advantageous for producing quality fruits during the growth and development of plants. METHODS AND RESULTS: We measured the rates of scab incidence among seedlings in a field experiment condition and an in-vitro test. An in-vitro test has been done with field experiment-based results. We made plant materials by grafting branches of each seedlings with 'Kongbae' rootstocks. And they had been grown for one month. Then, scab conidia suspension is sprayed to seedlings and sustained for 40 days under the controlled environment. As the results, 6 seedlings displayed lower incidence rates than other seedlings and 'Niitaka'. We also measured instant photosynthetic rates of each seedlings to determine the correlation between photosynthetic rates and fruit characteristics. However, it seemed that there is no correlation between them. CONCLUSION(S): Among the seedlings, 6 seedlings displayed the higher resistance to scab than other seedlings and 'Niitaka'. This characteristics is considered to be come from the gene expression of European pear. And we found that photosynthetic rate in trees rarely does not influence the fruit characteristics. It is considered to be affected by cultivar's own characteristics.
Elder-berries are economical plants and grown commercially in the United States. Most of the crop is sold to processors and made into natural colour, jellies, jam, port wine or elder berry wine, elder berry juice or used to manufacture of pie mixes. Processors are showing increasing interest in cultivation elder berries. In the present study was wish to find the effect of various factors and practical methods on the processing for the true fruit colour made from fresh elder berries juice as the clearing by the treated pectinase, added sugar, vaccum concentration, extraction of anthocyanosid powder and its property with used prospects. In this study obtained results can be summarized as follows. 1. Pectinase is useful and effected to make clear juice from fresh elder berry juice treated on the $34{\sim}45^{\circ}C$. 2. P.V.C. drum is most useful stored without unfading for clearing elderberry juice and its exposed redish dark purple colour. 3. Clearing elderberry juice stored in the stainless steel drum, changed colour from redish dark purple to dark violets during the stored at the room temperature. 4. Added sugar (Brix $48{\sim}53^{\circ}C$) to the fresh elderberry juice is useful and suitable prospects for to make home juice and wine. 5. The elderberry juice of vaccum concentration is change colour from dark purple to black brown colour during the stored at the room temperature. 6. Its possible to make anthocyanosid powder extracted made from fresh elderberry juice 7. Property of anthocyanosid is an follows. 1) The spectrum of elderberries juice is $523{\sim}530m{\mu}$ 2) The colouring stability is less than $pH_4$ 3) The colouring stability by the heating is less than $pH_4$, and then the more than $pH_4$ is changed colour from redish dark purple to dark brown. 4) By the light colouring stability is less than $pH_4$ 5) The colouring most stability in the pH is $pH_3$ 8. In the future, natural colour made from elderberry juice on the useful prospects is suitable used to make natural fruit juice, ice cream, candy, commercial and home wine, gum, and manufacture of jelly jam, pie maxes, ect.
Mulberry plants (Morus alba L.) were grown in pots with the following different nitrogen sources: ammonium sulphate, urea, ammonium nitrate, sodium nitrate + ammonium nitrate ($NO_3:NH_4$=2:1), and sodium nitrate. The effects of the nitrogen sources on mulberry yields, nitrogen recovery, distribution of ions and cation-anion balance (C-A) along leaf sequence and growth stage were investigated. The results were as follows: 1. Leaf yields and nitrogen recovery decreased with increasing $NO_3$-N application rates. 2. Relative cation contents in leaves in the early growth stages showed the following pattern : Na < Mg < Ca < K. However, the order of Ca and K reversed in the later stages. The order of anion contents chifted from $SO_4$ < $NO_3$ < Cl < $H_2PO_4$ in the early stages to $NO_3$ < Cl < $SO_4$ < $H_2PO_4$ in the later stages. 3. Contents of K, $H_2PO_4$, $SO_4$, $NO_3$, T-N and the sum of anion contents (${\sum}A$) were higher in upper leaves whereas Ca, Mg, Cl, the sum of cation contents (${\sum}C$) and (C-A) were higher in lower leaves. 4. When $NO_3$ in leaves decreased, Cl and K as counter-cations increased and consequently Ca decreased. 5. The (C-A) in leaves varied with leaf sequence and growth stage from 700 to 900 me/kg D.M.
The causes of late establishment of barnyardgrass and their density effect on rice yield loss were examined in 1996 and 1977. Herbicide application on 5 to 15DAT(Days after transplanting) increased from 6% to 52.5%, 1988 to 1992, and their ingredient amount for barnyardgrass control decreased to 41.7-87.5% in Korea. Most late establishment of barnyardgrass in machine transplanted rice field were not late germinated but revived ones. The number of late established barnyardgrass were 0.5, 2.0 and 13.3/$m^2$, on direct seeded at May 10, and machine transplanted May 23 and June 9, individually. Relation on rice yield and the number of barnyardgrass at machine transplanted field showed highly significant equation, as y=543.3 4.7x, r=0.9039 in 1996 and y=515.8 10.4x+0.066$x^2$, $R^2$=0.9532 in 1997. Theoritical yield loss by regress equation showed 2% per one plant of barnyardgrass per $m^2$ and 5, 10, 20, 50, and 80 plants of barnyardgrass decreased rice to 10, 19, 35, 69 and 79%, individually.
Jo, Man Hyun;Ham, In Ki;Park, Kwon Seo;Cho, Ji Hong
KOREAN JOURNAL OF CROP SCIENCE
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v.65
no.4
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pp.468-476
/
2020
This study was performed to establish light intensity conditions for producing stem cuttings for aeroponic systems suitable for seed potato production using a closed-type plant production system. Shoot tip cultured plantlets of 'Sumi' and 'Chubaek' potato (Solanum tuberosum L.) were acclimatized, cuttings were collected, and stem cuttings were planted. The seedlings were raised for 40 days at different LED light intensities (60, 120, 180, and 240 μmol·m-2·s-1), and were cultivated in an aeroponic system for 80 days. When stem cuttings were raised at 60 μmol·m-2·s-1 LED light intensity, the plant height was the longest, at 17.3 cm for 'Sumi' and 16.1 cm for 'Chubaek', and the number of nodes was the highest in both cultivars. The higher light intensities, produced smaller plants with fewer nodes. The leaf areas, SPAD values, and Fv/Fm values differed slightly between cultivars. The fresh weight of stem cuttings, and the production rate of healthy stem cuttings were the highest at 60 μmol·m-2·s-1. In the aeroponic system, seedlings raised at 60 μmol·m-2·s-1 with LED light intensity showed a difference between the cultivars, but the fresh weight of stems and leaves above the planting plate was the heaviest. In addition, below the planting plate the stem cuttings were longest and the root weight was heaviest at 60 μmol·m-2·s-1 LED light intensity. The number of stolons also differed between cultivars, but was greatest for seedlings raised at 60 μmol·m-2·s-1 LED light intensity, at 4.2/plant for 'Sumi' and 7.7/plant for 'Chubaek'. At 60 μmol·m-2·s-1 LED light intensity, the tuber number and total tuber weight were the best, but the higher the light intensity, the smaller the total tuber number and total tuber weight for both cultivars. In conclusion, when producing potato stem cuttings for aeroponic systems using a closed-type plant production system, the most suitable LED light intensity for raising seedlings was found to be 60 μmol·m-2·s-1.
Park Jong-Chul;Lee Mi-Ja;Choi In-Bae;Kim Mi-Jung;Park Chul-Soo;Kim Jung-Gon
KOREAN JOURNAL OF CROP SCIENCE
/
v.51
no.5
/
pp.477-482
/
2006
Viral diseases, especially Barley yellow mosaic virus (BaYMV) and Barley mud mosaic virus (BaMMV), have been most serious in barley fields. In this study, we investigated the effect of different level of resistance to viral diseases on the plant growth and yield in barley. Various viral disease symptoms on leaves of overwintered plants were similar between medial-resistant and susceptible varieties of Saessalbori and Baegdong. In diagnosis of virus infection, BaYMV and BaMMV were detected in Saessalbori and Baegdong, but not in the resistant variety, Naehanssalbori. Plant height was restrained about $11{\sim}12cm$ prior to heading in Saessalbori and Baegdong comparing to Naehanssalbori. Even if both varieties were medial resistant to virus diseases, Saessalbori was different from Baegdong in heading date and culm length due to its recovery from viral damages prior to heading. Both medial-resistant and susceptible varieties were quite different from the resistant variety in yield components such as heading date, number of spikes and culm length when evaluated in the virus-infected or non-infected field. Baegdong delayed 7 days in heading date and reduced by more than 50% in culm length and spike numbers as compared to Naehanssalbori. On the other hand, Saessalbori showed similar heading date, but was shorter by 20% in culm length than Naehanssalbori. Three varieties tested in the non-infected field over two years were not significantly different for yield potential with ranges of $340{\sim}405kg/10a$. However, significant yield reduction (P<0.01) was observed in Saessalbori and Baegdong with ranges of $108{\sim}288kg/10a$ as compared to Naehanssalbori (391 kg/10a) when tested in the virus-infected field. Yield potentials of Saessalbori and Baegdong reduced by 35 and 63%, respectively in the virus-infected field as compared to those in the non-infected field. Our results showed that damages from virus diseases were significant on the early plant growth to yield and its components in barley.
The bidding phase for logistics outsourcing services is critical for both shippers and logistics companies. According to the logistics bidding phase, the shipper should provide logistics operation information to logistics companies to resolve uncertainty. In addition, the logistics company can win the contract volume that was placed in the bid by expressing their experience and know-how, and proposing to share the risks and benefits of the shipper's logistics operation. Therefore, it is necessary to examine the factors that can be identified during the bidding phase for logistics outsourcing and how these factors affect the satisfaction of logistics outsourcing services. Based on the factors identified in the preceding studies on logistics outsourcing partnership factors and those on logistics outsourcing determinants, a survey was conducted on experts engaged in logistics companies, performing logistics for domestic shippers and analyzed using Smart-PLS. This study presents the following implications. First, in the logistics bidding phase, the shipper should provide its logistics operation information to logistics firms to resolve uncertainties. Details An in-depth explanation of the operation details will be presented via the bidding presentation, and on-site tours of manufacturing plants and logistics centers should also be carried out if necessary. Second, in the bidding phase, logistics companies should appeal through proposals to their competitiveness, such as experience and knowledge of the logistics of the shipper, and also consider alliances with other logistics companies to supplement their insufficient logistics services. Third, logistics companies should make proposals to share profits and risks through logistics outsourcing during the bidding phase, propose accepting risks from environmental uncertainties of the shipper within its capacity to an acceptable extent, and share the benefits of carrying out the shipper's logistics.
Kim, Dong-Min;Ju, Hong-Guang;Yang, Paul;Han, Seong-Sook;Roh, Jae-Hwan;Ahn, Sang-Nag
Korean Journal of Breeding Science
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v.43
no.1
/
pp.42-49
/
2011
QTL analysis for blast resistance was carried out using 140 $BC_3F_3$ lines derived from a cross between Ilpum as a recurrent parent and Moroberekan as a donor parent. 140 $BC_3F_3$ lines with the parents were inoculated with nine blast isolates. To identify QTLs for resistance to nine blast isolates, 134 SSR markers showing polymorphisms between the parents were genotyped for the 140 $BC_3F_3$ lines. A total of 17 resistance QTLs to nine isolates were detected on chromosomes 2, 3, 4, 6, 7, 9 and 10. The phenotypic variance explained by each QTL ranged from 8.2% to 26.4%. The Moroberekan alleles contributed the positive effect at these 17 QTL loci. In a previous study, the QTL, Pi45(t) for durable resistance to blast was identified using a sequential planting method. To know the relationship between Pi45(t) and the isolate-specific resistance gene, an $F_2$ population was developed from a cross between Ilpum and an introgression line harboring Pi45(t). $F_3$ lines segregating for the Pi45(t) were inoculated to three isolates. $F_3$ lines from the $F_2$ plants with the Moroberekan segment at the target region showed resistance to two isolates. This result seems to indicate that the Pi45(t) and the isolate-specific resistance gene are tightly linked or the resistance is controlled by the same gene(s). The markers linked to genes controlling blast resistance would be useful in developing blast resistance lines in the breeding program.
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