• Asian-Australasian Journal of Animal Sciences
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• 제19권4호
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• pp.495-499
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• 2006

In our previous studies, we demonstrated that Vascular Endothelial Growth Factor (VEGF) enhances bovine oocyte maturation and early embryonic development in serum supplemented media. In this experiment, to determine the synergistic effect of VEGF with serum components on early embryonic development in vitro in cattle, 1 mg/ml polyvinyl-alcohol (PVA) was replaced with foetal bovine serum (FBS) in maturation and culture media. Bovine oocytes were matured in Synthetic Oviduct Fluid (SOF) supplemented with PVA, PVA+5 ng/ml of VEGF, FBS, or FBS+VEGF. Fertilized oocytes were cultured in the same conditions for 8 days. The development of embryos was examined at 48 h post- insemination and on days 6, 7 and 8. The results were analyzed using repeated measures two- factor ANOVA, in which the effects of VEGF and serum were assigned as two factors. The development rate to 4- to 8-cell embryos at 48 h was significantly higher in the PVA+VEGF group than in the PVA group (44.7% and 31.5%, respectively). However, the highest development rate to 4- to 8-cell embryos was obtained from the FBS+VEGF group (58.8%). On day 8, the blastocyst rates were higher in the PVA+VEGF (22.8%), FBS (32.1%, p<0.05) and FBS+VEGF (42.1%, p<0.05) groups than in the PVA group (17.1%). Two- factor ANOVA of the development rates indicates that VEGF had a significant effect, but had no synergistic effect with serum components on early embryonic development. The results of the present study demonstrate that VEGF improves the in vitro developmental competence of bovine oocytes and/or embryos independent of the effect of serum components.

• Journal of Acupuncture Research
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• 제33권1호
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• pp.47-56
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• 2016

Objectives : This study examined the effects of Bee venom on apoptosis in NCI-H157 human lung cancer cells and for promoting the apoptosis effects of Natural killer cell. Methods : Bee venom and Natural killer-92 cells were cultured either separately from or together with NCI-H157 cells for 24 hours. To figure out whether Bee venom enhances the cytotoxic effect of Natural Killer-92 cells, a cell viability assay was conducted. To observe the changes in Death receptors, apoptotic regulatory proteins and Nuclear $Factor-{\kappa}B$, western blot analysis was conducted. To observe the effect of Bee venom through an extrinsic mechanism, a transfection assay was conducted. Results : 1. Natural killer-92 cells and Bee venom significantly inhibited the growth of NCI-H157 cells and co-culture had more inhibitory effect than the separate culture. 2. Expressions of Fas, DR3, DR6, Bax, caspase-3, caspase-8, cleaved caspase-3, cleaved caspase-8 were increased, and expressions of Bcl-2 and cIAP were decreased. More efficacy was observed in co-culture than in separate culture. 3. Nuclear $Factor-{\kappa}B$ activation was clearly decreased. And co-culture showed much less activation than separate culture. 4. As a result of treatment for DR-siRNA, the reduced cell viability of NCI-H157 cells and the activity of Nuclear $Factor-{\kappa}B$ were increased. With this, it can be seen that Bee venom and Natural killer-92 cells have an effect on the cancer cells through the extrinsic mechanism. Conclusion : Bee venom is effective in inhibiting the growth of human lung cancer cells. Furthermore Bee venom effectively enhances the functions of Natural killer cells.

• 한방안이비인후피부과학회지
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• 제19권3호통권31호
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• pp.34-54
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• 2006

The effects of four extracts of medicinal herbs, Rubus coreanum, Acorus calamus, Morus alba and Rehmannia glutinosa on hair growth activity and acnes control were investigated. In the course of screening natural extracts for hair growth, we found that the extract of dried root of Rubus coreanum has the hair growth promoting effect. After topical application of these extracts to the back of C57BL/6 mice, the earlier conversion of telogen-to-anagen phase was induced. The growth of dermal papilla cells and mouse vibrissae hair follicle cultured in vitro, however, was not affected by treatment of these extracts. Furthermore these extracts do not possesspotent inhibitory effect on $5{\alpha}-reductase$ I and II activity and anti-bacterial effect on Escherichia coli , Propionibacterium acnes, Pityrosporum ovale, Staphylococcus aureus, Staphylococcus epidemidis, and Candida albicans. RT-PCR analysis showed that these extracts did notinduce mRNA levels of growth factors such as insulin-like growth factor-I, keratinocyte growth factor, hepatocyte growth factor and vascular endothelial growth factor in dermal papilla cells. These results suggest that Rubus coreanum has hair growth promoting effect. However, the effects of these materials on the hair growth promotion are not mediated through inhibition of $5{\alpha}-reductase$ I and II activity, stimulation of hair follicle cells and expression of growth factors in the dermal papilla cells.

• 대한통합의학회지
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• 제8권4호
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• pp.163-169
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• 2020

Purpose : This study aimed to investigate the anti-allergic effect of Persicaria perfoliata water extract (PPWE) on IgE stimulated rat basophilic leukemia (RBL-2H3) cell line. Methods : P. perfoliata (L.) H. Gross has been used in traditional medicine as an anti-allergic agent, antipyretic, and diuretic and for respiratory disorders. To analyze the anti-allergic activity of PPWE, release of β-hexosaminidase in RBL-2H3 cells was estimated by enzyme linked immunosorbant assay (ELISA). Also, the cytotoxic effect of PPWE was identified by WST assay, and nuclear factor (NF)-κB and its upstream signaling molecules were assessed by western blot analysis. Results : PPWE treatment significantly attenuated β-hexosaminidase release in a dose dependent manner without any cytotoxicity. PPWE inhibited β-hexosaminidase activity by 38.4±1.2, 36.6±0.6, 32.5±2.2 and 26.5±1.2 at 500, 250, 100, and 50 ㎍/㎖ of PPWE, respectively, compared with the control group. In addition, an analysis of the expression level of NF-κB, an inflammation transcription factor, in RBL-2H3 cells upon IgE stimulation provided reults consistent with the results of β-hexosaminidase release. The phosphorylated status of upstream signaling molecules for transcription factor, mitogen activated protein kinases (MAPKs), was also analyzed. The results showed that PPWE treatment dose-dependently inhibited phosphorylation of extracellular regulatory kinase (ERK) and c-Jun N-terminal kinase (JNK). These results show that PPWE had a strong IgE-mediated degranulation inhibitory effect on RBL-2H3 cells. Conclusion : P. perfoliata ameliorated IgE-mediated allergic reaction via the modulation of MAPK and NF-κB signaling pathway in RBL-2H3 cells. These results indicate that P. perfoliata could be a potential candidate for a treatment strategy against various allergic disorders.

• 디지털융복합연구
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• 제16권6호
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• pp.63-73
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• 2018

본 연구는 중소 제조 및 서비스업체를 대상으로 하여 KMS의 경영성과에 미치는 영향요인을 지식정보요인과 관리 요인으로 설정하여 이들 요인들이 경영성과에 미치는 영향을 분석하였고, 평가와 보상을 조절변수로 설정하여 평가와 보상이 지식정보요인 및 관리요인과 경영성과 간에 미치는 조절적 효과를 분석하였다. 분석결과, 지식 적합성, 지식정보의 신뢰성, 지식관리프로세스 지원으로 설정된 지식정보 요인은 경영성과에 정의 영향을 미치는 것으로 분석되었고, 관리요인 중에서는 학습의 조직화와 협력 요인은 경영성과에 정의 영향을 미치는 것으로 분석되었다. 평가와 보상이 지식정보요인 및 관리요인과 경영성과 간에 조절적인 역할을 하고 있는지를 검증한 결과에서는 지식정보요인의 지식 적합성, 지식정보의 신뢰성, 지식관리프로세스지원, 관리요인의 지식공유활동, 협력은 경영성과에 조절적인 역할을 하고 있는 것으로 분석되었다.

• 한국정보통신학회논문지
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• 제16권9호
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• pp.2015-2020
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• 2012

본 연구에서는 이중게이트(Double Gate; DG) MOSFET에 스켈링이론을 적용할 때 두 개의 게이트에 의한 효과를 반영하기 위하여 스켈링인자에 가중치를 적용하여 문턱전압이하 특성을 해석하였다. 포아송방정식에 의한 전위분포를 구하기 위하여 전하분포는 가우스분포함수를 이용할 것이며 이의 타당성은 이미 여러 논문에서 입증하였다. 이 전위분포를 이용하여 단채널효과 중 문턱전압이동, 문턱전압이하 스윙, 드레인유도장벽감소 등을 스켈링인자에 대한 가중치의 변화에 따라 관찰하였다. 이중게이트 MOSFET의 구조적 특성상 채널길이에 대한 가중치는 0.1에서 1까지 사용하였으며 채널두께에 대한 가중치는 1에서 2까지 가중치를 사용하였다. 결과적으로 문턱전압 이하 스윙은 스켈링인자에 따라 거의 변화가 없었으나 가중치에 따라 변화하였으며 문턱전압이동 및 드레인유도 장벽감소 등은 스켈링인자에 따라 그리고 가중치에 따라 큰 변화를 보이는 것을 알 수 있었다.

• 대한조선학회논문집
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• 제50권5호
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• pp.291-297
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• 2013

Generally, form factor is determined through ITTC method. Determining the form factor from ITTC method includes the assumption that the form factor of a full-scale ship is the same value as its model ship. In other words, the form factor is independent on Reynolds number. However, for the more appropriate prediction of the resistance performance of a full-scale ship, the form factor must be determined with the consideration of the variation attendant on Reynolds number. In this research, several Geosim ship models are adopted to investigate the scale effect, and correlation lines of form factor are improved to suggest the better extrapolation method for the prediction of the form factor of full-scale ship. The corrected form factors using the correlation lines are compared with those determined from the results of low-speed resistance tests. To consider the influence of hull form, the correlation lines are determined for the group of high-speed ships and the group of low-speed ships, respectively. The corrected form factors have shown good agreement among the prediction results from each Geosim ship model to the full-scale ship.

• 한국방사선학회논문지
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• 제15권3호
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• pp.281-291
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• 2021

본 연구는 자기공명영상을 이용한 TOF MRA 검사에서 flow rate 2.0 ml 이하의 유속을 표현함에 있어 Compressed sensing의 사용에 따른 영상의 변화를 정량적으로 측정하고자 하였다. Auto-injector와 Flow phantom을 이용하여 각각의 혈류속도 구간을 설정하고 CS를 사용하지 않은 TOF without CS 기법과 CS를 이용한 TOF with CS 기법에서 CS factor의 변화에 따른 SNR, CNR, SSIM, RMSE 등을 측정하여 비교하였다. CS factor의 증가에 따라 나타나는 영상의 영향을 검증하고자 일원배치 분산분석(One-way ANOVA)을 시행하였다. 실험 결과 CS를 사용한 TOF MRA는 CS를 사용하지 않은 TOF MRA와 비교하여 SNR 및 CNR의 유의한 차이 없이 scan time이 현저하게 감소하였다. 반면 CS factor의 증가에 따라 SSIM 및 RMSE는 TOF without CS 영상과의 차이가 증가함을 나타내었다. 따라서 TOF MRA 검사 시 CS 기법을 통해 scan time을 효율적으로 감소시키되 적절한 CS factor의 범위를 충분히 고려해야 한다. 또한 CS factor와 영상의 유사도, 정밀성에 대한 지속적인 연구가 필요할 것으로 사료된다.

• The Korean Journal of Physiology and Pharmacology
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• 제26권6호
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• pp.469-478
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• 2022

WNT signaling plays an important role in cardiac development, but abnormal activity is often associated with cardiac hypertrophy, myocardial infarction, remodeling, and heart failure. The effect of WNT signaling on regulation of atrial natriuretic peptide (ANP) secretion is unclear. Therefore, the purpose of this study was to investigate the effect of Wnt agonist 1 (Wnta1) on ANP secretion and mechanical dynamics in beating rat atria. Wnta1 treatment significantly increased atrial ANP secretion and pulse pressure; these effects were blocked by U73122, an antagonist of phospholipase C. U73122 also abolished the effects of Wnta1-mediated upregulation of protein kinase C (PKC) β and γ expression, and the PKC antagonist Go 6983 eliminated Wnta1-induced secretion of ANP. In addition, Wnta1 upregulated levels of phospho-transforming growth factor-β activated kinase 1 (p-TAK1), TAK1 banding 1 (TAB1) and phospho-activating transcription factor 2 (p-ATF2); these effects were blocked by both U73122 and Go 6983. Wnta1-induced ATF2 was abrogated by inhibition of TAK1. Furthermore, Wnta1 upregulated the expression of T cell factor (TCF) 3, TCF4, and lymphoid enhancer factor 1 (LEF1), and these effects were blocked by U73122 and Go 6983. Tak1 inhibition abolished the Wnta1-induced expression of TCF3, TCF4, and LEF1 and Wnta1-mediated ANP secretion and changes in mechanical dynamics. These results suggest that Wnta1 increased the secretion of ANP and mechanical dynamics in beating rat atria by activation of PKC-TAK1-ATF2-TCF3/LEF1 and TCF4/LEF1 signaling mainly via the WNT/Ca²⁺ pathway. It is also suggested that WNT-ANP signaling is implicated in cardiac physiology and pathophysiology.

• Geomechanics and Engineering
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• 제33권2호
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• pp.141-154
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• 2023

This study investigated the effect of water retention characteristics between aged and fresh Municipal Solid Waste (MSW) on the stability of the landfill. A series of transient numerical modeling for the slope of an MSW landfill was performed considering the variation of water retention characteristics due to leachate circulation. Four different scenarios were considered in this analysis depending on how to obtain hydraulic conductivity and the aging degree of materials. Unsaturated hydraulic properties of the MSW used for the modeling were evaluated through modified hanging column tests. Different water retention properties and various landfill conditions, such as subgrade stiffness, leachate injection frequency, and gas and leachate collection system, were considered to investigate the pore water distribution and slope stability. The stability analyses related to the factor of safety showed that unsaturated properties under those varied conditions significantly impacted the slope stability, where the factor of safety decreased, ranging between 9.4 and 22%. The aged materials resulted in a higher factor of safety than fresh materials; however, after 1000 days, the factor of safety decreased by around 10.6% due to pore pressure buildup. The analysis results indicated that using fresh materials yielded higher factor of safety values. The landfill subgrade was found to have a significant impact on the factor of safety, which resulted in an average of 34% lower factor of safety in soft subgrades. The results also revealed that a failed leachate collection system (e.g., clogging) could result in landfill failure (factor of safety < 1) after around 298 days, while the leachate recirculation frequency has no critical impact on stability. In addition, the accumulation of gas pressure within the waste body resulted in factor of safety reductions as high as 24%. It is essential to consider factors related to the unsaturated hydraulic properties in designing a landfill to prevent landfill instability.