• Journal of Environmental Science International
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• v.29 no.5
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• pp.435-443
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• 2020

The purpose of this study was to investigate eco-friendly measures to manage major diseases which cause heavy economic damages to ginseng. Morphological, physicochemical, and molecular biological species identification was carried out after isolating useful antagonistic bacteria from ginseng fields. In addition, optimal conditions for mass culture were established, and he efficacy of the bacteria in the prevention of the diseases was verified in the field. The results showed that about 150 bacteria were extracted from 150 ginseng fields in the whole county. Among them, B. pyrrocinia LA101 was finally selected, which had a strong antagonistic potency against Alternaria panax, Botrytis cinerea, Rhizoctonia solani, and Cylindrocarpon destructans on agar media. The B. pyrrocinia LA101 is a baculiform gram-negative bacterium identified as Burkholderia pyrrocinia according to results from an API(Analytical Profile Index) kit, 16S rRNA, and gyrase gene sequencing analysis. It was donated to the microbe bank of the Agricultural Genetic Resources Center at the National Academy of Agriculture Science under the Rural Development Administration on September 28, 2011 (Donation No. KACC91663P). A patent for the mass culture technology was granted in August 2012 (Patent No. 10-1175532).

• Proceedings of the Korea Water Resources Association Conference
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• 2018.05a
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• pp.477-477
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• 2018

하천의 제방은 하천수의 범람을 방지하기 위하여 인공적으로 만든 구조물로서, 유수의 흐름을 일정하게 하고 자연재해에 대하여 제내지를 보고하기 위한 목적도 있다. 제방은 제내지와 제외지의 수위차로 인해 발생하는 파이핑, 제방의 월류, 사면의 탈락 등으로 파괴가 발생되고, 제방의 파괴는 제내지의 인명, 토지, 건물 등에 많은 피해가 발생한다. 이러한 제방의 파괴를 방지하기 위하여 제방 사면의 호안, 차수벽, 수제 등을 설치하고 있으며, 구조물 외에도 제방 응급복구를 위한 모래주머니, 비탈면 보호 시설 등을 이용한 피해방지에 대한 대책을 세우고 있다. 제방 구조물의 대부분은 콘크리트를 이용한 고강도 재료를 사용하여 설치되고 있으며, 재료의 환경성에 대한 문제점이 대두되어 왔다. 이에 따라 코코넛껍질을 이용한 사면 보호공, 고강도 식생매트를 이용한 사면 보호 등 친환경 재료를 이용한 사면보호 공법에 대한 연구들이 다양하게 진행되어 왔다. 본 연구에서는 미생물을 이용하여 사면을 보호하기 위한 블록 및 피복기법에 대한 연구를 진행하고 있으며, 다양한 환경성 및 무독성 검증도 병행중에 있다. 그 중 본 연구에서는 신소재와 황토, 모래, 석고를 다양한 비율로 조합하여 블록으로 활용하기 위한 일축압축 강도 실험을 수행하였으며, 신소재의 소류력 저항능력을 실험하기 위하여 안동 하천실증연구센터의 급경사 수로에 신소재를 피복한 시험구를 조성하고 5m/s 이상의 유속에서 저항능력 실험을 수행하였다. 본 연구에서는 본 실험 결과를 기반으로 하여 추후 다양한 친환경 재료를 이용한 조합을 통해 기존 콘크리트 블록에 준하는 강도를 발현할 수 있는 신소재 블록의 개발을 목표로 하고 있다.

• Parasites, Hosts and Diseases
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• v.58 no.6
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• pp.661-668
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• 2020

In Europe, 5 Lipoptena species have been recorded, including Lipoptena fortisetosa. This species, native to Asian countries, was described as a parasite of sika deer and its appearance in Europe dates back to more than 50 years ago. Lipoptena fortisetosa has been recently reported in Italy, sharing its hosts with Lipoptena cervi. A morpho-molecular approach was developed to determine the phylogenetic interrelationship of Italian and Asian CO1 haplotypes sequenced from Lipoptena fly individuals collected in Italy, and their DNA sequences were compared with conspecifics available in GenBank; morphological key-characters (terminalia) of L. fortisetosa were compared with the original description. Two haplotypes were recorded from Italy and assigned to L. cervi and L. fortisetosa, respectively. The latter was part of the monophyletic clade L. fortisetosa, along with 2 Central European and 2 Korean haplotypes (100% identical to one of the Korean haplotypes); moreover, Italian L. fortisetosa female terminalia were consistent with the original description of Asian individuals. Pending more in-depth investigations, this study provides a first answer to the hypothesis of the recent colonization of Italy by L. fortisetosa from Asia as we did not detect any obvious and stable morphological and molecular differences in specimens from the 2 geographical areas. The presence of the sika deer in Europe was retraced and the possible route traveled by the parasite from Asia and the eco-biological factors that may have enhanced its settlement are discussed.

• The Plant Pathology Journal
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• v.37 no.6
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• pp.580-595
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• 2021

Although late blight is an important disease in ecofriendly potato cultivation in Korea, it is highly dependent on the use of eco-friendly agricultural materials and the development of biological control technology is low. It is a necessary to develop an effective biocontrol agent to inactivate late blight in the field. AFB2-2 strain is a gram-positive with peritrichous flagella. It can utilize 20 types of carbon sources, like L-arabinose, and D-trehalose at 35℃. The optimal growth temperature of the strain is 37℃. It can survive at 20-50℃ in tryptic soy broth. The maximum salt concentration tolerated by AFB2-2 strain is 7.5% NaCl. AFB2-2 strain inhibited the mycelial growth of seven plant pathogens by an average inhibitory zone of 10.2 mm or more. Among the concentrations of AFB2-2, 10⁷ cfu/ml showed the highest control value of 85.7% in the greenhouse. Among the three concentrations of AFB2-2, the disease incidence and severity of potato late blight at 10⁷ cfu/ml was lowest at 0.07 and 6.7, respectively. The nucleotide sequences of AFB2-2 strain were searched in the NCBI GenBank; Bacillus siamensis strain KCTC 13613, Bacillus velezensis strain CR-502, and Bacillus amyloliquefaciens strain DSM7 were found to have a genetic similarity of 99.7%, 99.7%, and 99.5%, respectively. The AFB2-2 strain was found to harbor the biosynthetic genes for bacillomycin D, iturin, and surfactin. Obtained data recommended that the B. velezensis AFB2-2 strain could be considered as a promising biocontrol agent for P. infestans in the field.

• Journal of the Korean GEO-environmental Society
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• v.13 no.1
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• pp.45-51
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• 2012

This article aims to find method to mix a harmless hardening agent and soil generated during construction to make paving materials. The main purpose of this research is to get rid of the harmfulness(Chromium (VI), etc.) of cement which has been generally and frequently used as a hardening agent and strengthen it so that it can be used for the general foundation solidification and stabilization of civil engineering/construction structures such as dredging soil treatment, marine structure foundation treatment, surface soil stabilization, and river bank erosion prevention. NSS(Natural Stabilizer Soil) used for this study takes as its chief ingredient the mixture of lime and staple fibers extracted from natural fibers. It increases the shearing strength of soil that it improves the support and durability of the foundation and prevents flooding and frost as well. The pH measured to know its eco-friendliness was 6.67~7.15, and according to the migration testing, only Pb and CN were lower than the standards, so it can be said that NSS has almost no harmful components in it. According to the result of uniaxial strength testing, when the mixture ratio of weathered soil to NSS was 6%, about 1,850kpa strength was expressed. And according to the result of CBR. testing to figure out its appropriateness as a paving material, the CBR of the foundation was 4%~6%. But when the mixture ratio of NSS is over 6%, the water immersion CBR. is over 100%; thus, it is expected that it will show great utility as a paving material.

• Korean Journal of Environment and Ecology
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• v.25 no.6
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• pp.836-845
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• 2011

This study aims feeding habit of the Korean water deer(Hydropotes inermis argyropus) using a visual analysis of rumen contents of 44 individuals. The samples were collected from Cheorwon(Gangwon province) and the eastern part of Jeonnam province. Based on the analysis, a total of 15 orders, 18 families and 13 species were identified. Among them, Solanceae(53.8%) and Graminease(30.3%) showed the highest proportions. Plants of 13 orders, 15 families and 10 species were found and forbs(70.21%) and Solanceae(54.9%) were most preferred in Cheorwon. Eight orders, 7 families and 4 species were found in the Eastern parts of Jeonnam province, and Gramineae(78.6%) and woody plants(14.70%) was most preferred. Among four seasons, Gramineae in spring(80.6%) and autumn(68.8%), Leguminosae(75.9%) in summer, and Solanceae(85.4%) in winter were the highest proportions.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.18 no.6
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• pp.151-165
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• 2015

This study surveys and analyzes the ecology of Gangseo area to be developed near Nakdonggang River and West Nakdonggang River in Busan - before its development. With the result of the surveys as its basis, the study also finds ways to conserve the area's bird habitats in response to the planned development. According to the site surveys over four seasons, West Nakdonggang River which is near the targeted development area is a wintering ground for migratory birds. Some representative bird types in this area are ducks, geese, and other water birds. There are even six legally protected species. The average altitude of their flight is calculated to be $92.4{\pm}18.8m$ and the main purpose for the migration is determined to be for food. According to evalution of the conservation value of the area, Nakdonggang River and West Nakdonggang River turns out to have the highest conservation value. Other areas are developable as long as it was environmentally friendly. Therefore, this study suggests incorporation of ecological parks and biotopes within the targeted development area along with the proposal for spaces in the surroundings where the wintering birds can rest and feed. Especially for the wintering birds that rest at Nakdonggang River and West Nakdonggang River, their flight direction and pattern was examined to determine that their resting ground should be located 50~100m off of the river bank. Furthermore, this study proposes ways to maximize bird habitat by building ecological corridors between the agricultural channel and other small streams in the targeted development area and the habitats in the surroundings. Lastly, the study suggests the construction of low buildings, at most 20m in height, near Nakdonggang River and West Nakdonggang River considering the birds' flight altitude and direction.

• Korean Journal of Environment and Ecology
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• v.24 no.3
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• pp.318-324
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• 2010

The aim of this study is to examine feeding habits of the Korean water deer(Hydropotes inermis argyropus) from its rumen contents using a PCR-DGGE method. For this study, rumen contents were collected from water deer causalities by natural death or road-kill in two different sites(Cheorwon, Gangwon province and the Eastern part of Jeonnam province). DNA was extracted from rumen contents of a total of 44 individuals. Two primers, rbcLZ1aF(GC) and rbcL19bR, were used for PCR amplifications of ribulose-1,5-bisphosphate carboxylase large subunit (rbcL) gene. Among 44 samples, twenty-nine samples were successfully amplified by PCRs. The 29 PCR products of partial rbcL gene were applied for PCR-DGGE. Totally, six families of plants were detected from the diet analyses. Five families of plants were found in Cheorwon, Gangwon province, but only three families of plants were found in the Eastern part of Jeonnam province. The PCR-DGGE method will provide us with a potential tool to study feeding habits of ungulates including water deer, even though our results failed to identify the prey plants at the level of species.

• Journal of Life Science
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• v.14 no.1
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• pp.21-25
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• 2004

To identify genes involved in the decolorization of both crystal violet and malachite green, we isolated random mutants generated by transposon insertion in triphenylmethane-decolorizing bacterium, Citrobacter sp. The resulting mutant bank yielded 14 mutants with complete defect in color removal capability of both crystal violet and malachite green. Southern hybridization with a Tn5 fragment as a probe showed a single hybridized band in 5 mutants and these mutants appeared to have insertions at different sites of the chromosome. Tn5-inserted genes were isolated and the DNA sequence flanking Tn5 was determined. From comparison with a sequence database, putative protein products encoded by cmg genes were identified as follows. cmg 2 is MaIC protein in maltose transport system; cmg 6 is transcriptional regulator (LysR-type): cmg 12 is a putative oxidoreductase. The sequences deduced from two cmg genes, cmg 8 and cmg 11, showed no significant similarity to any protein with a known function. Therefore, these results indicate that these two cmg genes encode unidentified proteins responsible for decolorization of both crystal violet and malachite green.

• Ecology and Resilient Infrastructure
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• v.2 no.2
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• pp.147-153
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• 2015

Various adverse effects can occur due to direct exposure from toxic substances when toxic materials are used to restore river ecosystems. Thus, this study performed analysis on the development of toxicity in terms of survival and abnormality rates using embryos of Bombina orientalis living in Korea to analyze the toxicity of materials used in the river projects. The results showed that the toxicity in cement (C group) was the strongest whereas the toxicity in plant-based polyurethane (P1 group) was the weakest. Survival rates of B. orientalis embryos were 100%, 94 - 95%, 66 - 89% and 0% in control, P1, polyurethane (P2) and C groups, respectively. Abnormalities of embryos were 10.5%, 5.3 - 10.5%, 26.3 - 27.8% and 35.7% in control, P1, P2 and C groups, respectively. Furthermore, we verified that having a sufficient curing time reduced toxic substances that were extracted. The above result suggest that cement and polyurethane hamper the early development of amphibians. In conclusion, it is highly important to review biological safety with respect to the selection of materials used to restore rivers. This study shows the importance of the selection of eco-friendly materials and processes.