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A Study of Potential of Diet Analysis in the Korean Water Deer(Hydropotes inermis argyropus) using Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis(PCR-DGGE)

Park, Ji-Eun (Division of EcoScience, Ewha Womans University)
Kim, Baek-Jun (Conservation Genome Resource Bank for Korean Wildlife(CGRB), College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University)
Lee, Sang-Don (Department of Environmental Science and Engineering, College of Engineering, Ewha Womans University)

Publication Information

Korean Journal of Environment and Ecology / v.24, no.3, 2010 , pp. 318-324 More about this Journal

Abstract

The aim of this study is to examine feeding habits of the Korean water deer(Hydropotes inermis argyropus) from its rumen contents using a PCR-DGGE method. For this study, rumen contents were collected from water deer causalities by natural death or road-kill in two different sites(Cheorwon, Gangwon province and the Eastern part of Jeonnam province). DNA was extracted from rumen contents of a total of 44 individuals. Two primers, rbcLZ1aF(GC) and rbcL19bR, were used for PCR amplifications of ribulose-1,5-bisphosphate carboxylase large subunit (rbcL) gene. Among 44 samples, twenty-nine samples were successfully amplified by PCRs. The 29 PCR products of partial rbcL gene were applied for PCR-DGGE. Totally, six families of plants were detected from the diet analyses. Five families of plants were found in Cheorwon, Gangwon province, but only three families of plants were found in the Eastern part of Jeonnam province. The PCR-DGGE method will provide us with a potential tool to study feeding habits of ungulates including water deer, even though our results failed to identify the prey plants at the level of species.

Keywords

WATER DEER; FOOD; KOREA; MOLECULAR METHOD; CHLOROPLAST GEN;

Citations & Related Records

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