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http://dx.doi.org/10.5352/JLS.2004.14.1.021

Identification of Genes Involved in Decolorization of Crystal Violet and Malachite Green in Citrobacter sp.  

Lee, Young-Mi (College of Natural Resources and Life Science, Dong-A University)
Jang, Moon-Sun (College of Natural Resources and Life Science, Dong-A University)
Kim, Seok-Jo (College of Natural Resources and Life Science, Dong-A University)
Park, Yong-Lark (College of Natural Resources and Life Science, Dong-A University)
Cho, Young-Su (College of Natural Resources and Life Science, Dong-A University)
Lee, Young-Choon (College of Natural Resources and Life Science, Dong-A University)
Publication Information
Journal of Life Science / v.14, no.1, 2004 , pp. 21-25 More about this Journal
Abstract
To identify genes involved in the decolorization of both crystal violet and malachite green, we isolated random mutants generated by transposon insertion in triphenylmethane-decolorizing bacterium, Citrobacter sp. The resulting mutant bank yielded 14 mutants with complete defect in color removal capability of both crystal violet and malachite green. Southern hybridization with a Tn5 fragment as a probe showed a single hybridized band in 5 mutants and these mutants appeared to have insertions at different sites of the chromosome. Tn5-inserted genes were isolated and the DNA sequence flanking Tn5 was determined. From comparison with a sequence database, putative protein products encoded by cmg genes were identified as follows. cmg 2 is MaIC protein in maltose transport system; cmg 6 is transcriptional regulator (LysR-type): cmg 12 is a putative oxidoreductase. The sequences deduced from two cmg genes, cmg 8 and cmg 11, showed no significant similarity to any protein with a known function. Therefore, these results indicate that these two cmg genes encode unidentified proteins responsible for decolorization of both crystal violet and malachite green.
Keywords
Transposon mutagenesis; Decolorization; Triphenylmethane dye; Citrobacter sp.;
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