• 한국의류산업학회지
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• 제21권6호
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• pp.708-716
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• 2019

This study explored Jeju natural resources with healing function and tried mixed dyeing of Ecklonia cava and onion shell to improve dyeing. The results showed that silk dyed with a mixture of Ecklonia cava powder and onion shells rather than Jeju's individually dyed silk had better color power to develop fashion products. In addition, all friction lightning bolts, dry cleaning and sweat mining levels were excellent as well as excellent UV protection rates. The three baenaet jeogori worn during a birth anniversary and four rest suits that infants and children can wear comfortably maintained the dyeing and functioning of Ecklonia cava and onion shell mixture that was proven through abnormal experiments. For the three baenaet jeogori and two rest suits, the company has developed a fashion product recognized for high value-added merchandise using a variegation dyeing method made in a handmade style for expressing a person's natural sensibility through dyeing fabrics. Based on the results of the above research, it is expected that various fashion product developments will be possible by dyeing a mixture of functional and colorful extracts of the drug and colorful dye.

• 한국수산과학회지
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• 제42권5호
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• pp.523-526
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• 2009

Biodegradable polybutylene succinate (BPS) multifilament were designed to degrade upon disposal by the action of afforestration of Ecklonia cava. Matured thalli of E. cava were collected at Jeju for zoospore collection and the substrate for zoospores were BPS multifilament (12 mm, 500 Td/96). The materials were made as nets of $1\;m^2$ with a cross stripes of 10 cm. The unit biodegradable nets bearing germlings of E. cava was moved into Wando where the place is conducting intensive seaweed cultivation in Korea for 5 months of nursery culture until they grew to 10 cm in length after which the nets were transplanted into the sea bed at Jeju at a depth of 12 m and the algal growth was monitored from May 2007 to December 2008. This is the first instance of using the BPS materials for seaweed afforestation to avoid any environmental problems.

• Fisheries and Aquatic Sciences
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• 제14권3호
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• pp.198-204
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• 2011

The brown seaweed Ecklonia cava is known to be a rich source of phlorotannins that have diverse biological activities. Among the phlorotannins in E. cava, concentrations of dieckol and phlorofucofuroeckol-A, which were identified as major active components, were determined in different parts of the tissue. We compared the efficacy of different pretreatments for their extraction. A high-performance liquid chromatography (HPLC) method to determine phlorotannin concentrations showed good accuracy (92.64 and 94.02%, respectively), precision (3.92 and 3.94%, respectively), and linearity (r>0.996). Mature thalli contained 1.5-fold more dieckol (1.82 mg/g-dry tissue) than young thalli. In the tissues of E. cava, blade tissue contained more phlorotannins than the stipe or holdfast. Among differently dried thalli, approximately 90% or more dieckol and phlorofucofuroeckol-A were extracted from shadow-dried tissue as compared with lyophilized tissue. In sun-dried and oven-dried thalli, approximately 60% of the phlorotannins were extracted. Thalli washed with fresh water, boiled thalli, and steamed thalli showed reduced extraction of the compounds.

• Nutrition Research and Practice
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• 제5권2호
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• pp.93-100
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• 2011

Inhibition of angiotensin I-converting enzyme (ACE) activity is the most common mechanism underlying the lowering of blood pressure. In the present study, five organic extracts of a marine brown seaweed Ecklonia cava were prepared by using ethanol, ethyl acetate, chloroform, hexane, and diethyl ether as solvents, which were then tested for their potential ACE inhibitory activities. Ethanol extract showed the strongest ACE inhibitory activity with an $IC_{50}$ value of 0.96 mg/ml. Five kinds of phlorotannins, phloroglucinol, triphlorethol-A, eckol, dieckol, and eckstolonol, were isolated from ethanol extract of E. cava, which exhibited potential ACE inhibition. Dieckol was the most potent ACE inhibitor and was found to be a non-competitive inhibitor against ACE according to Lineweaver-Burk plots. Dieckol had an inducible effect on the production of NO in EAhy926 cells without having cytotoxic effect. The results of this study indicate that E. cava could be a potential source of phlorotalnnins with ACE inhibitory activity for utilization in production of functional foods.

• Preventive Nutrition and Food Science
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• 제10권2호
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• pp.134-139
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• 2005

Seven brown algal species (Ecklonia cava, Ishige okamurae, Sargassum fulvellum, Sargassum horneri, Sargassum coreanum, Sargassum thunbergii and Scytosiphon lomentaria) were hydrolyzed using five proteases (Protamex, Kojizyme, Neutrase, Flavourzyme and Alcalase) and screened for angiotensin 1-converting enzyme (ACE) inhibitory activities. Most algal species examined showed good ACE inhibitory activities after the enzymatic hydrolysis. However, E. cava was the most potent ACE inhibitor of the seven species. Flavourzyme digest of E. cava exhibited an $IC_{50}$ of around $0.3\;{\mu}g/mL$ for ACE; captopril has an $IC_{50}$ of $\~0.05\;{\mu}g/mL$. The Flavourzyme digest was separated to three fractions by an ultrafiltration membrane (5, 10, 30 kDa MWCO) system according to the molecular weights. The active components were mainly concentrated in >30 kD fraction which are composed of the highest protein content $(27\%)$ and phenolic content (261 mg/100 mL) compared to the other two smaller molecular weight fractions. Therefore, the active compounds appear to be relatively high molecular weight complex molecules associated with protein (glycoprotein) and polyphenols. Therefore, E. cave is a potential source of antihypertensive compound.

• 한국수산과학회지
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• 제47권4호
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• pp.363-369
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• 2014

Obesity is a worldwide problem that contributes to serious diseases, including diabetes, hypertension, and atherosclerosis. Recently, much research has examined functional natural materials and their anti-obesity activity. This study investigated the effect of enzyme-treated Ecklonia cava extracts on mice fed a high fat diet. To test the anti-obesity effects of a diet containing the enzyme-treated E. cava extracts (EEc), C57BL/6NTacSam mice were divided into six groups : normal diet (ND), high-fat diet (HFD), high-fat with Garcinia extract diet (GHD), and three high-fat with EEc diet (EHD250, EHD500, and EHD1000) groups. After 9 weeks, body weight was increased significantly in the HFD group compared to all of the EHD groups, and the weights of the liver, perirenal fat and epididymal fat paralleled the increase in body weight. The serum GOT, GPT, triglyceride, total cholesterol, and LDL-cholesterol levels were lower in the EHD1000 group than in the HFD group. The glucose and leptin concentrations were lowest in the EHD1000 group and C/EBP family expression was decreased in a dose-dependent manner. These results indicate that E. cava extracts not only have anti-oxidation functions but also anti-obesity effects.

• 한국수산과학회지
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• 제48권3호
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• pp.301-307
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• 2015

Phlorotannins and marine algal polyphenols, including dieckol, 6,6-bieckol, phloroglucinol, phlorofucofuroeckol-A, and eckol, were isolated from brown seaweeds. These compounds have beneficial bioactivities, and Ecklonia cava has become widely used for the extraction and isolation of phlorotannins. Eckol, in particular, has been to shown to have antioxidant, anti-inflammatory, anticoagulatory, and photoprotective properties. However, due to its low abundance in weaweed, the isolation and purification of eckol are difficult. Its limited availability renders the isolation and purification of eckol labor-intensive processes. Centrifugal partition chromatography (CPC) is an efficient technique for the isolation and purification of eckol. In this study, eckol was isolated from the ethyl acetate fraction of the 70% ethanol extract of E. cava using CPC with a two-phase solvent system of a n-hexane:EtOAc:methanol:water (2:8:3:7, v/v) solution. The purity and anti-inflammatory activity of the isolated eckol were verified by high-performance liquid chromatography and by assaying lipopolysaccharide-induced inflammatory responses in an immortalized murine BV2 microglial cell line, respectively. In conclusion, CPC is a useful technique for simple and efficient isolation of eckol from E. cava.

• ALGAE
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• 제34권2호
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• pp.177-185
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• 2019

Ecklonia cava is popular in Korea as a marine functional materials. E. cava is generally collected and used on the coast of Jeju Island. However, the continuous use of collected natural E. cava may be limited because difficult to secure throughout the year and may be exposed to environmental pollution. Jeju magma seawater (MSW) was known to be significant advantages such as safety, cleanness, stability, and functional improvement. Attempts have been reported on application of MSW to the culturing of macro- and microalgae and showed improved results. Thus, the objective of the present study was to explore the anti-melanogenesis activity of brown seaweed E. cava (E. cava cultured with MSW [MSWE]) extract cultured in tanks with MSW of Jeju Island to evaluate the possibility of cosmeceutical industrial application. MSWE extract showed the higher polyphenolic and dieckol contents than natural E. cava (NE) extract. Anti-melanogenesis activity of MSWE extract and NE extract are tested and compared using tyrosinase and dihydroxyphenylalanine (DOPA) oxidation inhibition assay. MSWE extracts evidenced more effective tyrosinase and DOPA oxidation inhibition activity than that of the NE extracts and the commercial whitening agent, arbutin. MSWE extracts also markedly inhibited melanin synthesis and decreased the expression of melanogenesis-related protein in ${\alpha}$-melanocyte stimulating hormone-stimulated B16F10 melanoma cells without cytotoxicity. These results suggest that MSW cultivation process would be more effective in releasing bioactive compounds with whitening effect from seaweed such as E. cava at an industrial scale.

• 한국식품과학회지
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• 제51권5호
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• pp.503-508
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• 2019

감태(Ecklonia cava) 추출물의 in vitro 항산화효과 및 초미세먼지로 유도된 세포 독성에 대한 비강(RPMI-2650), 폐(A549) 및 뇌신경(MC-IXC) 세포에서의 세포보호효과를 검증하였다. 감태는 총 페놀성화합물(물 추출물: 189.32, 80% 에탄올 추출물: 418.08 mg GAE/g of dried weight) 및 총 플라보노이드(물 추출물: 44.93, 80% 에탄올추출물 111.22 mg RE/g) 함유량을 각각 나타내었으며, ABTS/DPPH 라디칼 소거활성 및 지방질과산화 억제효과에서도 우수한 효과를 나타냈다. 또한, 감태 추출물은 초미세먼지로 유도될 수 있는 세포 내 산화적스트레스를 저해함으로써 세포 사멸을 효과적으로 억제시킬 수 있는 천연 소재로 판단되며, 80% 에탄올 추출물뿐만 아니라 물 추출물 또한 산업적 활용가능성이 기대된다.

• Journal of Evidence-Based Herbal Medicine
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• 제2권1호
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• pp.1-5
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• 2009

Objectives : The purpose of this study was to investigate the anti-inflammatory effects of Ecklonia cava butanol extract (BFEC) on RAW 264.7 cells. Method : To evaluate of anti-inflammatory of BFEC, We examined cytokine and Nitric oxide(NO) production in lipopolysacchride (LPS)-induced RAW 264.7 cell. Result : Extract of BFEC inhibit LPS-induced interleukin (IL)-6, NO production in human monocyte RAW 264.7 cells. Conclusion : BFEC down-regulated LPS-induced IL-6, NO production, which may be provide a clinical basis for anti-inflammatory properities of BFEC.