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http://dx.doi.org/10.4162/nrp.2011.5.2.93

Effect of phlorotannins isolated from Ecklonia cava on angiotensin I-converting enzyme (ACE) inhibitory activity  

Wijesinghe, W.A.J.P. (School of Marine Biomedical Sciences, Jeju National University)
Ko, Seok-Chun (School of Marine Biomedical Sciences, Jeju National University)
Jeon, You-Jin (School of Marine Biomedical Sciences, Jeju National University)
Publication Information
Nutrition Research and Practice / v.5, no.2, 2011 , pp. 93-100 More about this Journal
Abstract
Inhibition of angiotensin I-converting enzyme (ACE) activity is the most common mechanism underlying the lowering of blood pressure. In the present study, five organic extracts of a marine brown seaweed Ecklonia cava were prepared by using ethanol, ethyl acetate, chloroform, hexane, and diethyl ether as solvents, which were then tested for their potential ACE inhibitory activities. Ethanol extract showed the strongest ACE inhibitory activity with an $IC_{50}$ value of 0.96 mg/ml. Five kinds of phlorotannins, phloroglucinol, triphlorethol-A, eckol, dieckol, and eckstolonol, were isolated from ethanol extract of E. cava, which exhibited potential ACE inhibition. Dieckol was the most potent ACE inhibitor and was found to be a non-competitive inhibitor against ACE according to Lineweaver-Burk plots. Dieckol had an inducible effect on the production of NO in EAhy926 cells without having cytotoxic effect. The results of this study indicate that E. cava could be a potential source of phlorotalnnins with ACE inhibitory activity for utilization in production of functional foods.
Keywords
Angiotensin I-converting enzyme; dieckol; Ecklonia cava; functional foods; phlorotannins;
Citations & Related Records
Times Cited By KSCI : 8  (Citation Analysis)
Times Cited By Web Of Science : 3  (Related Records In Web of Science)
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