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Determination of Nutrient Contents of Liquid Pig Manure and the Correlation of Components as Fertilizer in Western JeJu Area (제주 서부지역 양돈장에서 생산된 돈분액비의 비료성분과 그 성분간 상관관계)

  • Song Sang-Taek;Kim Mun-Chol;Hwang Kyoung-Jun
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.26 no.1
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    • pp.15-24
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    • 2006
  • This study was conducted to make a rapid and easy determination for the fertility of liquid pig manure as fertilizer by investigating the contents, and correlation coefficients of various nutrients. Samples were collected from 118 local pig farms in the western area of Jeju in Korea. Electrical conductivity(EC), dry matter(DM), $NH_4-N$ and minerals were determined and the relationships among them were examined. The collected liquid manure samples from 118 pig farms were classified according to the level of DM contents ;< 3% (92 farms), $3{\sim}6%$ (18 farms), $6{\sim}9%$ (5 farms) and>9% (3 farms), based on the collected data, most of the liquid manure coming from the local pig farms contain small amount of dry matter. The dry matter contents appeared highly correlated(p<0.01) with EC, $NH_4-N$, T-P, Ca, Mg and Na, except for K. In addition EC was proportional to $NH_4-N$, T-P, Ca, and Na except fer Mg. The fertilizer component ratio of $NH_4-N$, P and K in liquid pig manure were not constant, resulting in low efficiency for fertilizer. However, the toxic heavy metals of Cu etc. were below the criteria of organic fertilizer and soil contamination evaluation. Therefore, we concluded that both dry matter content and electrical conductivity could be used as an indicator for evaluating the fertility of liquid pig manure.

Seasonal Characteristics of Organic Carbon and Elemental Carbon in PM2.5 in Daejeon (대전지역 대기 중 PM2.5의 유기탄소와 원소탄소의 계절별 특성 연구)

  • Kim, Hyosun;Jung, Jinsang;Lee, Jinhong;Lee, Sangil
    • Journal of Korean Society for Atmospheric Environment
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    • v.31 no.1
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    • pp.28-40
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    • 2015
  • To investigate the seasonal variations of carbonaceous aerosol in Daejeon, OC (organic carbon), EC (elemental carbon) and WSOC (water soluble organic carbon) in $PM_{2.5}$ samples collected from March 2012 to February 2013 were analyzed. $PM_{2.5}$ concentrations were estimated by the sum of organic matter ($1.6{\times}OC$), EC, water-soluble ions ($Na^+$, $NH_4{^{+}}$, $K^+$, $Mg^{2+}$, $Ca^{2+}$, $Cl^-$, $SO_4{^{2-}}$, $NO_3{^{-}}$). The estimated $PM_{2.5}$ concentrations were relatively higher in winter ($29.50{\pm}12.04{\mu}g/m^3$) than those in summer ($13.72{\pm}6.92{\mu}g/m^3$). Carbonaceous aerosol ($1.6{\times}OC+EC$) was a significant portion (34~47%) of $PM_{2.5}$ in all season. The seasonally averaged OC and WSOC concentrations were relatively higher in winter ($6.57{\times}3.48{\mu}gC/m^3$ and $4.07{\pm}2.53{\mu}gC/m^3$ respectively), than those in summer ($3.07{\pm}0.8{\mu}gC/m^3$, $1.77{\pm}0.68{\mu}gC/m^3$, respectively). OC was correlated well with WSOC in all season, indicating that they have similar emission sources or formation processes. In summer, both OC and WSOC were weakly correlated with EC and also poorly correlated with a well-known biomass burning tracer, levoglucosan, while WSOC is highly correlated with SOC (secondary organic carbon) and $O_3$. The results suggest that carbonaceous aerosol in summer was highly influenced by secondary formation rather than primary emissions. In contrast, both OC and WSOC in winter were strongly correlated with EC and levoglucosan, indicating that carbonaceous aerosol in winter was closely related to primary source such as biomass burning. The contribution of biomass burning to $PM_{2.5}$ OC and EC, which was estimated using the levoglucosan to OC and EC ratios of potential biomass burning sources, was about $70{\pm}15%$ and $31{\pm}10%$, respectively, in winter. Results from this study clearly show that $PM_{2.5}$ OC has seasonally different chemical characteristics and origins.

A Study on the Effect of Herbal-acupuncture with Eucomiae Cortex Solution at Joksamni$(ST_{36})$ on Collagen-induced Arthritis (족삼리(足三里) 두충약침(杜沖藥鍼)이 Collagen-induced Arthritis에 미치는 영향)

  • Kang, Jae-Hui;Lee, Hyun
    • Journal of Acupuncture Research
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    • v.23 no.3
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    • pp.129-142
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    • 2006
  • Objectives : The purpose of this study is to observe the effects of Eucomiae Cortex herbal-acupuncture solution(EC-HAS) at Joksamni(ST36) on arthritis of mice induced by Collagen II. Methods : The author performed several experimental items. First, it is the cell survival rate of mice lung fibroblasts. Second, it is the incidence rate of arthritis and arthritis index of CIA. Third, it is the levels of IL-6, $TNF-{\alpha}$, $IFN-{\gamma}$, $IL-{\beta}$, IgG, IgM and anti-collagen II in serum and the level of IFN-y,$IFN-{\gamma}$/IL -4 ratio in CIA mouse spleen cell culture. Fourth, it is histological analysis of the mice joint. Fifth, it is expression ratio of $CD3e^+$ to $CD19^+$+ cell, $CD4^+$ to $CD8^+$ cell, $CD69^+/CD3e^+$/cells, $CD11a^+/CD19^+$/cells, $CD11b^+/Gr-1^+$ cells and $CD4^+/CD25^+$ cells. Results & Conclusion : 1. In the EC-HA, the incidence of arthritis and arthritis index were significantly decreased. 2. In EC-HA, the levels of IL-6, $IFN-{\gamma}$, $TNF-{\alpha}$, $IL-1{\beta}$, IgG, IgM and anti-collagen II in serum of CIA mice and the level of $IFN-{\gamma}$, IL-4, $IFN-{\gamma}$/lL-4 ratio in CIA mouse spleen cell culture were significantly decreased. 3. In the histological study, the cartilage destruction and synovial cell proliferation were decreased in the EC-HA, and the collagen fiber expressions in the EC-HA were similar with that of the Normal group. 4. In the EC-HA, the expression ratio of $CD3e^+$ to $CD19^+$ cell and $CD4^+$ to $CD8^+$ cell were similarly maintained as Normal group in lymph nodes, and $CD69^+/CD3e^+$ cells and $CD11a^+/CD19^+$ cells were decreased in lymph nodes, and $CD11b^+/Gr-1^+$ cells and $CD4^+/CD25^+$ cells were decreased in synovium. These results suggest that EC-HA at ST36 has an effect to control synovial cell proliferation and cartilage destruction in rheumatoid arthritis, and to be put to practical use in the future rheumatoid arthritis clinic.

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Changes of Soil Chemical Properties and Uptake of Salts by the Plants according to the Application of the Food Waste Compost (음식물찌꺼기 퇴비의 시용에 따른 토양의 화학성 변화 밑 작물체내 염류의 흡수)

  • Lee, Sang-Suk;Chang, Ki-Woon
    • Journal of the Korea Organic Resources Recycling Association
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    • v.6 no.2
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    • pp.59-67
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    • 1998
  • This study was conducted to investigate the changes of soil chemical properties and uptake of salts by the plants(Raphanus and Lactuca) according to the application of the food waste compost(FWC), which was produced on a large scale at a pilot plant. Generally, FWC has a high electrical conductivity(EC) and contains much salts, such as Na, K, Ca, and Mg, and because of these when FWC are applied to soils there is a possibility of salt ac-cumulation in soils and growth inhibition on plants. The FWC were applied at the rates of 0, 20, 40, 80, and 160 Mg/ha in the 1/5,000a pots on the basis of dry weight, and the plants were cultivated for 60 days. And then, changes of pH and EC in soils and uptake of salts by the plants were investigated. The results obtained are summarized as follows; 1. After the cultivation of the Raphanus and Lactuca, pH increased and EC decreased in the soils. 2. Uptake rates of Na and K were slightly increased with the application of the FWC. In uptake rate of Ca, Raphanus and Lactuca was decreased, increased, respectively. In Raphanus, uptake rate of Mg was highest with the application of the FWC at 40 Mg/ha, and in Lactuca, continuously increased with application of the FWC.

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Effect of EC and pH of Nutrient Solution on the Growth and Quality of Single-Stemmed Rose in Cutted Rose Production Factory (절화장미 수경재배시 배양액의 농도와 pH가 생육과 품질에 미치는 영향)

  • Lee Hye Jin;Yang Eun-Young;Park Keum-Soon;Lee Yong-Beom;Bae Jong Hyang;Jeon Kyung Soo
    • Journal of Bio-Environment Control
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    • v.13 no.4
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    • pp.258-265
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    • 2004
  • The experiment was conducted to investigate the effect of EC and pH of nutrient solution on the growth and quality of the single-stemmed rose grown in a rose factory installed with hydroponic system. The growth and quality of the single-stemmed rose were not significantly different from each other with the EC of the nutrient solutions $1.0\~3.5dS{\cdot}m^{-1}$, which resulted in concluding high concentration of the nutrient solution was not necessary. The optimum range of the EC for single-stemmed rose was $1.5\~2.0dS{\cdot}m^{-1}$ considering plant growth, photosynthetic and transpiration rates. The optimum range of the pH for good plant growth without any visible physiological disorder was $4.0\~6.0$. Therefore, to keep the pH of the nutrient solution for rose low compared to other plants was beneficial for plant growth and uptake of the mineral ions.

Detection for the Resistance of Fusarium spp. Isolated from Rice Seeds to Prochloraz and Cross-resistance to Other Fungicides Inhibiting Sterol Biosynthesis (벼 종자에서 분리한 Fusarium속 균주들의 prochloraz에 대한 저항성 검정 및 교차 저항성 조사)

  • Shin, Myeong-Uk;Kang, Hyo-Jung;Lee, Yong-Hwan;Kim, Heung-Tae
    • The Korean Journal of Pesticide Science
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    • v.12 no.3
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    • pp.277-282
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    • 2008
  • To assess the resistance to prochloraz, $EC_{50}$ values of Fusarium isolates obtained from rice seed were investigated through the agar dilution method. $EC_{50}$ value of 36 isolates of Fusarium spp. to prochloraz ranged from 0.020 to $1.78{\mu}g\;mL^{-1}$ with an average of $0.25{\mu}g\;mL^{-1}$. According to the species of Fusarium, the average $EC_{50}$ value was fluctuated; $0.091{\mu}g\;mL^{-1}$ for F. moniliformis, $0.11{\mu}g\;mL^{-1}$ for F. proliferatum and $0.31{\mu}g\;mL^{-1}$ for F. fujikuroi. The resistant baseline was decided at $0.5{\mu}g\;mL^{-1}$ to determine if the isolate was resistant to prochloraz or not. Based on the resistant baseline, the ratio of resistant isolates was 14%. There was no correlation between the resistance to prochloraz and the pathogenicity of Fusarium spp. on rice seedlings. The resistant isolates of F. fujikuroi did not show the cross-resistance to other sterol biosynthesis inhibiting fungicides, triflumizole, hexaconazole, difenoconazole and tebuconazole.

Honeybee Acute and Residual Toxicity of Pesticides Registered for Strawberry (딸기용 농약의 꿀벌에 대한 급성독성 및 엽상잔류독성)

  • Kim, Byung-Seok;Park, Yoen-Ki;Lee, Yong-Hoon;Joeng, Mi-Hye;You, Are-Sun;Yang, Yu-Jung;Kim, Jin-Bae;Kwon, Oh-Kyung;Ahn, Young-Joon
    • The Korean Journal of Pesticide Science
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    • v.12 no.3
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    • pp.229-235
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    • 2008
  • Lack of honey bee toxicity data for most pesticide products used for strawberry restricts to predict the adverse effects to foraging honey bee after treatment of pesticide in plastic house. This study was conducted to evaluate the actual risk of worker honey bees (Apis mellifera L.) through acute contact toxicity test, acute oral toxicity test and toxicity of residues on foliage test with 21 pesticide products. The mortality of honeybee sprayed with 6 pesticides including dichlofluanid WP showed significantly different from control at recommended application rate in acute contact toxicity test at 24 hours after treatment. Fenpropathrin EC and milbemectin EC treatment groups showed more than 25% mortalities at recommended application rate in acute oral toxicity test. In toxicity of residues on foliage test, only fenpropathrin EC treatment group showed more than 25% mortalities at 10 days after treatment at recommended application rate. It was concluded that the most toxic route to exposure for honey bee is direct contact exposure to sprayed pesticides. Safety interval for honey bee was established by concerning the results of these tests.

Sperm-Associated Antigen 9 is a Promising marker for Early Diagnosis of Endometrial Cancer

  • Baser, Eralp;Togrul, Cihan;Ozgu, Emre;Ayhan, Sevgi;Caglar, Mete;Erkaya, Salim;Gungor, Tayfun
    • Asian Pacific Journal of Cancer Prevention
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    • v.14 no.12
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    • pp.7635-7638
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    • 2013
  • Background: Sperm-associated antigen 9 (SPAG9) has been recently proposed as a novel biomarker for early diagnosis of several human tumors, including ovarian, cervical and breast cancers. Its clinical value remains to be clarified for endometrial cancer (EC). In this study, we investigated the utility of serum SPAG9 levels in diagnosis of EC and its association with important clinicopathological parameters. Materials and Methods: This cross-sectional study was performed at a tertiary women's referral center in Ankara, Turkey. Preoperative serum samples were collected from patients surgically treated for endometrial cancer between June 2012-April 2013. Similar aged women with a biopsy proven benign endometrium were used as controls. Serum SPAG9 levels were measured with an enzyme-linked immunosorbent assay (ELISA) method and assessed for links with clinicopathological factors. Receiver operating characteristic (ROC) curve analysis was performed to assess power of SPAG9 levels for EC prediction. P values less than 0.05 were considered statistically significant. Results: A total of 63 women with EC and 27 with benign endometrium were included in the study. Mean age in the EC group was $58.7{\pm}1.1$. Median SPAG9 levels in the EC and control groups were 18.3 (range, 12.7-53.8) and 14.1 (range, 4.3-65.3), respectively (p<0.001). A cut-off value of 17 ng/ml for SPAG9 predicted presence of malignant endometrium with 74% sensitivity and 83% specificity [Area under curve (AUC)=0.82, p<0.001]. SPAG9 levels did not demonstrate any significant association with histological type, FIGO stage, tumor grade, size, myometrial invasion, lymphovascular space invasion, cervical involvement, adnexal involvement, peritoneal cytology or lymph node status (all p>0.05). Conclusions: Testing for SPAG9 may be useful for early detection of EC in asymptomatic high-risk women. Its role in post-treatment follow-up and early detection of recurrence should be assessed in future trials.

Effects of Salt-induced Stress on the Fluctuation and Rhizosphere Colonization of Soil Microorganisms (염류(鹽類)의 스트레스가 주요(主要) 토양미생물(土壤微生物)의 변동(變動) 및 근권정착성(根圈定着性)에 미치는 영향(影響))

  • Kwon, Jang-Sik;Suh, Jang-Sun;Weon, Hang-Yeon
    • Korean Journal of Soil Science and Fertilizer
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    • v.31 no.3
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    • pp.291-300
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    • 1998
  • A study was carried out to evaluate the effect of different salts and their Quantities on the fluctuation and rhizosphere colonization of soil microorganisms. The results obtained are as follows. The electrical conductivities(ECs) of $KNO_3$, $K_2HPO_4$, KCl and $K_2SO_4$ showed negative correlations to the number of gram positive bacteria and gram negative bacteria : the number of bacteria was significantly decreased in the KCl or $KNO_3$ treated group compared to the $K_2HPO_4$ or $K_2SO_4$ treated group. The highest microorganism density of gram negative bacteria, gram positive bacteria and Fusarium sp. in balanced salts-treated soil was observed at $0.5dS\;m^{-1}$, $2.1dS\;m^{-1}$ and $8.0dS\;m^{-1}$ of EC, respectively. The ratio of bacteria to fungi ratio in balanced salts-treated soil substantially decreased as the EC of soil increased. Ten and thirty days after soil treatment with balanced salts, the ratio of bacteria to fungi decreased to 757-1571 and 89-215, respectively. Root colonization density of Fluorescent Pseudomonas in cucumber and tomato significantly decreased as the EC of soil increased, whereas that of Fusarium sp. increased.

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Radiosensitivity Enhancement by Arsenic Trioxide in Conjunction with Hyperthermia in the EC-1 Esophageal Carcinoma Cell Line

  • Cui, Yan-Hui;Liang, Hai-Jun;Zhang, Qing-Qin;Li, Si-Qing;Li, Xiao-Rui;Huo, Xiao-Qing;Yang, Qing-Hui;Li, Wei-Wei;Gu, Jian-Fa;Hua, Qin-Liang;Lu, Ping;Miao, Zhan-Hui
    • Asian Pacific Journal of Cancer Prevention
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    • v.13 no.4
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    • pp.1693-1697
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    • 2012
  • Objective: To explore the effect on radiosensitivity of arsenic trioxide ($As_20_3$) in conjunction with hyperthermia on the esophageal carcinoma EC-1 cell line. Method: Inhibition of EC-1 cell proliferation at different concentrations of $As_20_3$ was assessed using the methyl thiazolyl blue colorimetric method (MTT method), with calculation of $IC_{50}$ value and choice of 20% of the $IC_{50}$ as the experimental drug concentration. Blank control, $As_20_3$, hyperthermia, radiotherapy group, $As_20_3$ + hyperthermia, $As_20_3$ + radiotherapy, hyperthermia + radiotherapy and $As_20_3$ + hyperthermia + radiotherapy groups were established, and the cell survival fraction (SF) was calculated from flat panel colony forming analysis, and fitted by the 'multitarget click mathematical model'. Flow cytometry (FCM) was used to detect changes in cell apoptosis and the cell cycle. Results: $As_20_3$ exerted inhibitory effects on proliferation of esophageal carcinoma EC-1 cells, with an $IC_{50}$ of 18.7 ${\mu}mol/L$. After joint therapy of $As_20_3$ + hyperthermia + radiotherapy, the results of FCM showed that cells could be arrested in the $G_2$/M phase, and as the ratio of cells in $G_0/G_1$ and S phases decreased, cell death became more pronounced. Conclusion: $As_20_3$ and hyperthermia exert radiosensitivity effects on esophageal carcinoma EC-1 cells, with synergy in combination. Mechanistically, $As_20_3$ and hyperthermia mainly influence the cell cycle distribution of EC-1 esophageal carcinoma cells, decreasing the repair of sublethal damage and inducing apoptosis, thereby enhancing the killing effects of radioactive rays.