• Proceedings of the CALSEC Conference
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• 2001.08a
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• pp.411-427
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• 2001

EC/SCM은 개별기업이 아닌 Grocery Supply Chain 전체의 효율제고를 위해 제조업체와 유통업체가 공동으로 소비자의 만족과 비용절감 극대화를 모색하는 ECR관점의 Grocery 산업 접근 방법임.(중략)

• 한국전기화학회:학술대회논문집
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• 2000.10a
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• pp.35-35
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• 2000

• Journal of Microbiology and Biotechnology
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• v.18 no.10
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• pp.1648-1654
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• 2008

Superoxide dismutase (SOD) removes damaging reactive oxygen species from the cellular environment by catalyzing the dismutation of two superoxide radicals to hydrogen peroxide and oxygen. Extracellular superoxide dismutase (EC-SOD) is a tetramer and is present in the extracellular space and to a lesser extent in the extracellular fluids. Increasing therapeutic applications for recombinant human extracellular superoxide dismutase (rEC-SOD) has broadened interest in optimizing methods for its purification, with a native conformation of tetramer. We describe a solid phase refolding procedure that combines immobilized metal affinity chromatography (IMAC) and gel filtration chromatography in the purification of rEC-SOD from Escherichia coli. The purified rEC-SOD tetramer from the $Ni^{2+}$-column chromatography is refolded in Tris buffer. This method yields greater than 90% of the tetramer form. Greater than 99% purity is achieved with further purification over a Superose 12PC 3.2/30 column to obtain the tetramer and specific activities as determined via DCFHDA assay. The improved yield of rEC-SOD in a simple chromatographic purification procedure promises to enhance the development and therapeutic application of this biologically potent molecule.

• Microbiology and Biotechnology Letters
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• v.39 no.1
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• pp.43-48
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• 2011

Physicochemical and organoleptic characteristics of Campbell Early red wines made by traditional method were investigated. The pH values of all Campbell Early red wines were ranged 3.0~3.3 during fermentation. The acidity value of Campbell Early red wine made by traditional method was 0.4~0.9%. Sucrose and alcohol contents were 6~7 Brix and 13.2~14.4% at the end of fermentation, respectively. Campbell Early red wine had the high sensory scores for color, aroma, taste, sharpness, after taste, and overall balance. Because Campbell Early red grapes are well fermented due to the rich fermentative sugar content, Campbell Early red wines made by adding EC-1118 yeast were shown to be the most appropriate.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.28 no.12
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• pp.2004-2011
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• 2004

The applicability of the ultrasonic C-scan inspection is restricted due to the deterioration of mechanical properties of specimen during the test. Therefore, the aim of this research is applied to Eddy Current (EC) test substitute for the C-scan inspection in CFRP tube containing defects. This research is to evaluate the EC signals for the inspection of CFRP tube containing various circular hole defects (20% to 100% depth to the specimen thickness) using the unloading specimen and radial loading specimen. This study was considered the following points; 1) Analysis of EC signals for the inspection of saw-cut defect and circular hole defect, 2) The evaluation of defect depths and EC signals relationship. 3) Variation of EC signal owing to the radial load. In conclusions, the high frequency such as 300∼500 kHz made it possible to the inspection of 40% to 100% defects. Particularly, in case of 20% defect, the EC signal was not detected due to the noise of micro-crack and delamination. While the depth of the hole defects were decreasing, the difference of the phase angle between unloading specimen and radial loading specimen was gradually increasing.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.9
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• pp.4607-4611
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• 2012

Objective: Endometrial cancer (EC) is the most common gynecologic malignancy. Identification of potential biomarkers of EC would be helpful for the detection and monitoring of malignancy, improving clinical outcomes. Methods: The Weighted Gene Co-expression Network Analysis method was used to identify prognostic markers for EC in this study. Moreover, underlying molecular mechanisms were characterized by KEGG pathway enrichment and transcriptional regulation analyses. Results: Seven gene co-expression modules were obtained, but only the turquoise module was positively related with EC stage. Among the genes in the turquoise module, COL5A2 (collagen, type V, alpha 2) could be regulated by PBX (pre-B-cell leukemia homeobox 1)1/2 and HOXB1(homeobox B1) transcription factors to be involved in the focal adhesion pathway; CENP-E (centromere protein E, 312kDa) by E2F4 (E2F transcription factor 4, p107/p130-binding); MYCN (v-myc myelocytomatosis viral related oncogene, neuroblastoma derived [avian]) by PAX5 (paired box 5); and BCL-2 (B-cell CLL/lymphoma 2) and IGFBP-6 (insulin-like growth factor binding protein 6) by GLI1. They were predicted to be associated with EC progression via Hedgehog signaling and other cancer related-pathways. Conclusions: These data on transcriptional regulation may provide a better understanding of molecular mechanisms and clues to potential therapeutic targets in the treatment of EC.

• Molecules and Cells
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• v.39 no.11
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• pp.814-820
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• 2016

FtsZ, a tubulin homologue, is an essential protein of the Z-ring assembly in bacterial cell division. It consists of two domains, the N-terminal and C-terminal core domains, and has a conserved C-terminal tail region. Lateral interactions between FtsZ protofilaments and several Z-ring associated proteins (Zaps) are necessary for modulating Z-ring formation. ZapD, one of the positive regulators of Z-ring assembly, directly binds to the C-terminal tail of FtsZ and promotes stable Z-ring formation during cytokinesis. To gain structural and functional insights into how ZapD interacts with the C-terminal tail of FtsZ, we solved two crystal structures of ZapD proteins from Salmonella typhimurium (StZapD) and Escherichia coli (EcZapD) at a 2.6 and $3.1{\AA}$ resolution, respectively. Several conserved residues are clustered on the concave sides of the StZapD and EcZapD dimers, the suggested FtsZ binding site. Modeled structures of EcZapD-EcFtsZ and subsequent binding studies using bio-layer interferometry also identified the EcFtsZ binding site on EcZapD. The structural insights and the results of bio-layer interferometry assays suggest that the two FtsZ binding sites of ZapD dimer might be responsible for the binding of ZapD dimer to two protofilaments to hold them together.

• Korean Business Review
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• v.12
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• pp.29-66
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• 1999

Electronic Commerce is rapidly expanding with the development of internet and will be very useful for the consumers in the future. However, we could not find many studies on consumers' attitudes and behaviors in the cybermarket. The purpose of this study is to analyze perceptions of college students in Seoul and Chunchon on Electronic Commerce. The main findings are as follows. First, they prefer surfing on the net with search engines like Simmamai or Yahoo to directly looking over the homepages of department stores and advertisement sites. Students in Chunchon, especially, tend to check out banners at E-mail sites. Second, most of the students get information about shopping mall sites and products from their friends, newspapers or magazines. And they want to have information (advertisements) classified by brand names of the products. Third, students in Seoul rely on brand names more than those in Chunchon do. Students in Chunchon, compared with those in Seoul, believe more kinds of products are available in the internet shopping mall. They complain, however, deficiencies of information about the products and high prices. They want the price information compared with those in traditional markets. Fourth, they feel very uncomfortable about informing their credit card numbers, and thus prefer payments with Electronic Money. And finally, they are not satisfied with return and exchange policies and after-sale services. They also complain that the products are not delivered safely when they are not home. They want the delivery-date indicated.

• Proceedings of the Korean Institute Of Construction Engineering and Management
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• autumn
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• pp.593-597
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• 2003

As construction projects are complicated and large in scale, a collaboration between project participants is getting more emphasized over the last few years. The collaboration tools for participants are various. but traditionally drawings and specifications have played an important role. Especially, though storage form in drawings vary in entire construction lifecycle, drawings are still important as the core factor in latest information technology streams in regard to usefulness and reusability of information. As an attempt to standardize drawings in the public construction based on CALS/EC, this study analyzes user's requirements for drawings qualitatively, and looks into drawings standards in Korea, America and Japan, and each kind of ISO standard for drawings. And this study predicts future-oriented user's requirement for drawings based on Construction CALS/EC, and presents future-oriented direction of development applicable in domestic drawing standard in construction.

• Korean Journal of Environmental Agriculture
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• v.28 no.4
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• pp.397-402
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• 2009

In Korea, salt stress is one of the major problems limiting crop production and eco-environmental quality in greenhouse soil. The objective of this study was to evaluate the effectiveness of organic residues (Chinese milk vetch, maize stalk, rice straw, and rye straw) for reducing salt activity in greenhouse soil. Organic residues was incorporated with salt-accumulated soil (EC, 3.0 dS $m^{-1}$) at the rate of 5% (wt $wt^{-1}$) and the changes of electrical conductivity (EC) was determined weekly for 8 weeks under incubation condition at $30^{\circ}C$. The EC, microbial biomass carbon (MBC), and water soluble ions in soil was strongly affected by C/N ratio of organic residues. After 8 weeks incubation, the concentration of water soluble $NO_3{^-},\;Ca^{2+}$, and $Mg^{2+}$ was significantly decreased in organic residues having high C/N ratio (maize stalk, rice straw, and rye straw) incorporated soil compared to organic residues having lower C/N ratio (Chinese milk vetch) incorporated soil. The EC value in Chinese milk vetch incorporated soil was higher than control treatment. In contrast, maize stalk, rice straw, and rye straw amended soil was highly decreased the EC value compared to control and Chinese milk vetch applied soil after 4 weeks incubation. Our results indicated that incorporation of organic residues having high C/N ratio (>30) could reduce salt activity resulting from reducing concentration of water soluble ions.