• Title/Summary/Keyword: Ec

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Production of Lignocellulytic Enzymes from Spent Mushroom Compost of Pleurotus eryngii (큰느타리버섯 수확 후 배지로부터 리그닌섬유소분해효소 생산)

  • Lim, Sun-Hwa;Kim, Jong-Kun;Lee, Yun-Hae;Kang, Hee-Wan
    • The Korean Journal of Mycology
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    • v.40 no.3
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    • pp.152-158
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    • 2012
  • The lignocellulytic enzymes including a-amylase (EC 3.2.1.1), lignin peroxidase (EC 1.11.1.14), laccase (EC 1.10.3.2), xylanase (EC 3.2.1.8), ${\beta}$-xylosidase (EC 3.2.1.37), ${\beta}$-glucosidase (EC 3.2.1.21) and cellulase (EC 3.2.1.4) were extracted from spent mushroom compost (SMC) of Pleurotus eryngii. Different extraction buffers and conditions were tested for optimal recovery of the enzymes. The optimum extraction was shaking incubation (200 rpm) for 2 h at $4^{\circ}C$. ${\alpha}$-Amylase was extracted with the productivity range from 1.20 to 1.6 Unit/SMC g. Cellulase was recovered with the productivity range from 2.10 to 2.80 U/gf. ${\beta}$-glucosidase and ${\beta}$-xylosidase productivities showed lowest recovery producing 0.1 U/g and 0.02 U/g, respectively. The P. eryngii SMCs collected from three different mushroom farms showed different recovery on laccase and xylanse, cellulase. Furthermore, the water extracted SMC was compared to commercial enzymes for its industrial application in decolorization and cellulase activity.

Optimal Extraction and Characteristics of Lignocellulytic Enzymes from Various Spent Mushroom Composts (다양한 버섯 수확 후 배지로부터 목질섬유소 분해효소의 최적 추출 및 특성)

  • Lim, Sun Hwa;Lee, Yun Hae;Kang, Hee Wan
    • The Korean Journal of Mycology
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    • v.41 no.3
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    • pp.160-166
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    • 2013
  • Recovery of ${\alpha}$-amylase (EC 3.2.1.1), lignin peroxidase (EC 1.11.1.14), laccase (EC 1.10.3.2), xylanase (EC 3.2.1.8), ${\beta}$-xylosidase (EC 3.2.1.37), ${\beta}$-glucosidase (EC 3.2.1.21) and cellulase (EC 3.2.1.4) from spent mushroom composts (SMCs) of Pleurotus cornucopiae, Pleurotus ostreatus, Pleurotus eryngii, Hericium erinaceum, Lyophyllum ulmarium, Agrocybe cylindracea, Lentinus lepideus, and Flammulina velvtipes were investigated using different extraction buffers. The maximum recovery of the enzymes was mostly detected in SMC extracts with tap water and 0.25% Triton X-100 by shaking incubation (200 rpm) for 2 h at $4^{\circ}C$. The xylanase (152 U/g) and laccase (8.1 U/g) activities were the highest in SMC extracts from F. velvtipes and P. eryngii. In addition, high enzymatic activities of ${\alpha}$-amylase (3.6 U/g) and cellulase (3.4 U/g) was detected in SMC extract of A. cylindracea. Futhermore, cellulase and laccase activities of SMCE from P. eryngii were compared to commercial enzymes.

Sensitivity to Fungicides of Typhula incarnata Isolates Causing Gray Snow Mold (설부소립균핵병균 Typhula incarnata의 살균제 감수성)

  • Lee, Seong Jun;Lee, Dong Woon;Chang, Taehyun
    • Weed & Turfgrass Science
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    • v.1 no.4
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    • pp.69-75
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    • 2012
  • In response of fungicides for control of gray snow mold, isolates of Typhula incarnata were collected from three golf courses in 2012 Yongpyeong, Korea and tested for sensitivity to propiconazole, tebuconazole and azoxystrobin fungicides. Four discriminatory concentrations were used to detect in vitro sensitivity of 50 isolates. Mean 50% effective concentration inhibiting mycelial growth ($EC_{50}$) values for tebuconazole was the lowest among the three fungicides. The $EC_{50}$ value of tebuconazole ranged from 0.0005 ${\mu}g\;ml^{-1}$ to 0.014 ${\mu}g\;ml^{-1}$ with a mean of 0.0048 ${\mu}g\;ml^{-1}$. The mean $EC_{50}$ values of propiconazole in triazole family was 0.5825 (0.78-1.651) ${\mu}g\;ml^{-1}$. $EC_{50}$ value of azoxystrobin ranged from 0.0017 ${\mu}g\;ml^{-1}$ to 0.131 ${\mu}g\;ml^{-1}$ with a mean of 0.0278 ${\mu}g\;ml^{-1}$. There was no correlation among $EC_{50}$ values for propiconazole, azoxystrobin and tebuconazole indicating no cross-resistance relationships with each other. Results of this study were confirmed no resistance isolates in vitro sensitivity of T. incarnata of three fungicides in Yongpyeong.

Acquisition and Analysis of Environmental Data for Smart Farm (스마트팜 생육환경 데이터 획득 및 분석)

  • Seok-Ho Han;Hoon-Seok Jang
    • The Journal of Korea Institute of Information, Electronics, and Communication Technology
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    • v.16 no.3
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    • pp.130-137
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    • 2023
  • Smart farms, which have been receiving attention as a solution to recent rural problems, refer to technologies that optimize the growing environment of crops and increase the productivity and quality of crops through efficient management. If the relationships between environmental data in smart farms are analyzed, additional productivity enhancement and crop management will be possible. In this paper, we propose a method for acquiring and analyzing nine environmental data, including temperature, humidity, CO2, soil temperature, soil moisture, insolation, soil EC, EC, and pH. Data acquisition is done through RS-485 communication between the main board and the sensor board and stored in the database after acquisition. The stored data is downloaded in Excel sheet format and analyzed through histograms, data charts, and correlation heatmaps. First, we analyze the distribution of total, day, and night data through histogram analysis, and identifiy the average, median, minimum, and maximum values by month through data chart analysis separating day and night to see how the data changes by month. Finally, we analyze the correlation of the data through a correlation heatmap analysis separating day and night. The results show a very strong positive correlation between temperature and soil temperature and soil EC and EC during the day, and a very strong positive correlation between temperature and soil temperature and soil EC and EC at night, and a strong negative correlation between temperature and soil EC.

Toxicity of Fungicides in vitro to Cylindrocarpon destructans

  • A.Monique Ziezold;Robert Hall;Richard D.Reeleder;John T.A.Proctor
    • Journal of Ginseng Research
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    • v.22 no.4
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    • pp.223-228
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    • 1998
  • As part of a study on the ability of fungicides to control disappearing root rot of ginseng (Panax quinquvdius) caused by Cylindruarpn destmtans, 15 fungicides were screened for toxicity to the fungus in vitro. Highly toxic fungicides were Benlate (benomyl), Thiram (thiram), and Orbit (propiconazole). EC5O values (mg a.i./L) were less than 1 and EC95 values were less than 10. Crown (carbathiin and thiabendazole), ASC-66835 (fluazinam), and UBI-2584 (tebuconazole) were moderately toxic, with EC5O values in the range 1-10 and EC95 values in the range 32-45. Weakly toxic fungicides (EC5O in the range 20-80, EC95 in the range 35-140) included UBI-2643 (thiabendazole), UBI-2565 (cyproconazole), and Vitaflo-280 (carbathiin and thiram). Anvil (hexaconazole), Vitaflo-250 (carbathiin), UBI-2383 (triadimenol), Daconil (chlorothalonil), CGA-173506 (fludioxonil), and CGA-169374 (difeno- conazole) were considered nontoxic to C. destmtan (EC5O 1.29->600, EC95>500). Relations between proportional inhibition of growth and concentration of fungicide were linear on arithmetic plots (Benlate, UBI-2643, UBI-2565, Vitaflo-280) or logarithmic plots (all other fungicides). Based on toxicity in vitro and formulation, it is recommended that Benlate, Orbit, and ASC-66835 be tested as soil drenches, and Benlate, Thiram, UBI-2584, and Crown be tested as seed treatments for controlling disappearing root rot.

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Moir'e fringes generated by the superposition of elongated circular grating and Talbot image and their applications (Elongated Circular Grating의 Talbot 결상을 이용한 무아레 무늬 발생과 응용)

  • Lee, Sang-Il;Jo, jae-Heung;Chang, Soo;Rim, Cheon-Seog
    • Korean Journal of Optics and Photonics
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    • v.12 no.1
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    • pp.25-31
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    • 2001
  • The Talbot effect of an elongated circular (EC) grating composed of a line grating and two semicircular gratings with well matched pitches is experimentally presented. As the relative positions between the fixed EC grating and the moving Talbot image of another EC grating or a self Talbot image are one-dimensionally deformed, we can visually observe moire fringes generated by their superposition. Two examples of their applications, that is, the measurement of the wedged angle of a wedged prism and of the fine rotational angle of a mirror are described.cribed.

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Exploratory Study and Empirical Study on Critical Website Success Factors of Chinese Publishing Enterprises

  • Huang Jinghua;Jiang Ximin;Lee Jingtin;Zhao Chunjun
    • Management Science and Financial Engineering
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    • v.11 no.3
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    • pp.109-124
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    • 2005
  • The study on the critical success factors (CSF) for electronic commerce systems has been a hot topic in both academe and industry. On the basis of reviewing papers on CSF and analyzing their problems, this paper designs the initial assessment indictors and website features and functions influencing EC success. Using Delphi survey and data analysis, we get the five important assessment indictors and seven important web features and functions. Furthermore, the hypothesis of CSF model is proposed. Finally, we conduct a survey on the Chinese Publishing Industry to test the hypothesis. The result shows that the hypothesis is partly supported, which means useful and understandable information, complete and timely information, credible and accurate information, all product-related information are the critical success factors for EC publishing industry. This research not only impels EC research in China, but also has instructional effect on the implementation of EC for enterprises to increase the success rate of EC.

A Study on the degradation mechanism of PAN-LiCLO$_4$ Polymer Electrolyte EC windows (PAN-LIClO$_4$ 계 고분자전해질 EC창의 열화 기구에 관한 연구)

  • 김용혁;김형선;조원일;조병원;윤경석;박인철
    • Journal of the Korean institute of surface engineering
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    • v.30 no.4
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    • pp.223-230
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    • 1997
  • Tungsten oxide and nickel oxide thin films were deposited onto ITO(Indium Tin Oxide) transparent glass by the E-beam evaporation and were used as a cathode and an anode for the EC(Electrochromic) smart window, respectively. Stoichiometric structures of the deposited films were investigated by the implementation of XPS(X-ray Photoelectron Spectroscopy) analysis and the results were $WO_{2.42}$ and $NiO_{0.44}$. This oxygen deficincy might affect affect the transparency of the thin films. The electrolyte for the EC smart windows was PAN-$LiCIO_4$ conducting polymer. EC(Ethylene Carbonate)and PC(Propylene Carbonate) were added as plasticizer to enhance ion conductivity. When the weight ratio of the EC : PC was 3 : 1, transmission difference and cycle life performance were tested. Polymer EC windows showed 40% $\Delta$T at 1.5V operating volage for 3,200 cycles. Structural degradation was observed by the SIMS(Secondary Ion Mass Spectroscopy) analysis and it was confirmed that structural degradation of polymer caused by the solvent evaporation was the main cause to degrade EC smart windows.

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Comparison between ACI 318-05 and Eurocode 2 (EC2-94) in flexural concrete design

  • Hawileh, Rami A.;Malhas, Faris A.;Rahman, Adeeb
    • Structural Engineering and Mechanics
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    • v.32 no.6
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    • pp.705-724
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    • 2009
  • The two major widely used building design code documents of reinforced concrete structures are the ACI 318-05 and Eurocode for the Design of Concrete Structures EC2. Therefore, a thorough comparative analysis of the provisions of these codes is required to confirm their validity and identify discrepancies in either code. In this context, provisions of flexural computations would be particularly attractive for detailed comparison. The provisions of safety concepts, design assumptions, cross-sectional moment capacity, ductility, minimum and maximum reinforcement ratios, and load safety factors of both the ACI 318-05 and EC2 is conducted with parametric analysis. In order to conduct the comparison successfully, the parameters and procedures of EC2 were reformatted and defined in terms of those of ACI 318-05. This paper concluded that although the adopted rationale and methodology of computing the design strength is significantly different between the two codes, the overall EC2 flexural provisions are slightly more conservative with a little of practical difference than those of ACI 318-05. In addition, for the limit of maximum reinforcement ratio, EC2 assures higher sectional ductility than ACI 318-05. Overall, EC2 provisions provide a higher safety factor than those of ACI 318-05 for low values of Live/Dead load ratios. As the ratio increases the difference between the two codes decreases and becomes almost negligible for ratios higher than 4.

A study on the degradation of the AC stressed MOV by using of the DLTS technique (DLTS기법에 의한 MOV소자의 교류과전경시 변화특성에 관한 연구)

  • 이동희
    • Electrical & Electronic Materials
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    • v.9 no.7
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    • pp.719-726
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    • 1996
  • DLTS measurements were performed to study the annealing induced changes of the trap centers in MOV and to shed more light on the stability mechanism of the MOV. Two electron traps, Ec-0.26[eV] and Ec-(O.2-0.3)[eV], were observed in the unannealed samples in large quantities(7-9 X 1014[CM 3]), whereas the three electron traps Ec-0.17 [eV], Ec-0.26[eV] and Ec-(O.2-0.3)[eV] were observed far less in the annealed samples. The minima in the Ec-0.26[eV] trap density, coupled with the presented results that unannealed devices are unstable whereas 600.deg. C annealed devices are most stable, suggests that the instability of the MOV under long term electrical stressing is related to the Ec-0.26[eV] trap. This results support that the ion migration model for the device instability where the Ec-0.26[eV] defects may be the interstitial zinc or the migrating ions. The interstitial zinc originated as a result of the nonstoichiometric nature of ZnO might cause the degradation of the I-V characteristics of the MOV with long term electrical stressing.

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