• Journal of Plant Biotechnology
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• v.44 no.1
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• pp.27-34
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• 2017

Our aim was to find the effective duration and culture conditions for microspore culture in a genetically variant cabbage (Brassica oleraceae L. var. capitata). We discovered that the '$2{\times}NLN$ medium containing $AgNO_3$ 1 mg/l, sucrose 13%' was most efficient in producing the cabbage embryos. The number of induced embryos was higher in $F_2$ or $F_3$ progeny generation than the $F_1$ hybrid cultivar used as plant materials. Thus, we recommend microspore culturing using progeny plants of failed $F_1$ cultivar. The acquisition ratio of embryos and plants derived from microspore was higher in the early flowering period as compared to the late flowering period. When transplanted in the soil, 71.2% plants developed from the early flowering period, compared to 27.0% from the middle period and 1.8% plants from the late period. Thus, we recommend microspore culture using buds collected during the early flowering period.

• Journal of Korean Society of Forest Science
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• v.45 no.1
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• pp.51-61
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• 1979

The chromosome behavior and it's synapsis in the meiosis of pollen mother cell were studied on Populus alba L. as a female parent tree, Populus glandulosa Uyeki as a male parent tree and their hybrid, Populus alba x glandulosa. 1. At metaphase I, the number of nuclear plates with early separation chromosome were observed with the lowest proportion of 11.0% in Populus glandulosa and with the highest proportion of 13.0% in Populus alba${\times}$glandulosa. 2. At metaphase II, early separation chromosomes appeared with the frequency of 11.0% in Populus alba${\times}$glandulosa. But the frequency was not different with those of the parental trees. 3. At anaphase I, lagging chromosomes appeared with some high rate of 11.6% in Populus alba${\times}$glandulosa and yet the number of chromosome bridges in populus alba x glandulosa almost were not different with the partental trees. 4. At anaphase II, lagging chromosomes appeared with some high frequency of 10.2% in Populus alba${\times}$glandulosa and the chromosome bridges in Populus glandulosa appeared with the highest frequency in all studied trees. 5. The frequency of abnormal pollen sporad was the highest value of 8.2% in Populus alba${\times}$glandulosa among the studied trees. With the results, it might be assured that the chromosome segregation and it's synapsis behaved normally in Populus alba, Populus glandulosa and Populus alba x glandulosa, and so all the studied trees could produced normal pollens.

• Journal of Forest and Environmental Science
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• v.11 no.1
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• pp.81-101
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• 1995

Random amplified polymorphic DNA (RAPD) technology, a recent approach in molecular genetics, is much usable to select the elite trees and to maximize the genetic gain in forest tree breeding program, providing a clue to determine the genetic marker-trait correlation. This review intorduces research on bark thickness and breeding strategy in Pinus elliottii, Pinus caribaea and their hybrid by Queensland Forest Service and ForBio Research Pty Ltd, University of Queensland, which employ RAPD technology. Genetic linkage map of $F_1$ hybrids includes 186 RAPD markers and 16 linkage groups (1641 cM long in total) and 6 quantitative trait loci are located putatively for bark thickness. Following recent research results and experiences in pine breeding programs, the forseeable stages in the application and development are proposed for marker assisted selectin; stage 1-determination of species specific markers for genes controlling traits of commercial interest, and stage 2-determination of marker-allele association for specific allelic variants within pure species. As pines inherit their megagametophytes from the seed parent and zygotic embryos from both male and female parents, the determination of marker-trait correlation is possible even in embryo stage, eventually making ways for the early selection of elite individuals.

• Journal of Animal Environmental Science
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• v.12 no.1
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• pp.35-40
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• 2006

This experiment was conducted to investigate the effect of application level of swine slurry on the growth characteristics and yield of sorghum$\times$sudangrass hybrid and $NO_3-N$ content in infiltration at experimental field of Grassland and Forage Crops Division, National Livestock Research Institute, RDA from 2000 to 2002. Treatments were consisted of non fertilizer(NF), chemical fertilizer(CF), 100% swine slurry(SS 100), 150% swine slurry(SS 150), 200% swine slurry(SS 200) and 100% swine slurry + CF 50%(SS100 + CF 50) with randomized complete block design and three replications. Growth of sorghum$\times$sudangrass hybrid was not nearly different among the treatments, but early growth of swine slurry treatments was better than that of CF, and regrowth after 1st cutting was shown better in CF and SS 100+CF 50 with adding application of chemical fertilizer. The sugar content(brix %) was tends to be increased with swine slurry application. Dry matter(DM) yields of SS 100 and SS 150 were lower 15 and 6% than that of CF, respectively, and SS 200 was similer to CF, but there was not found significant difference among all treatments. The content of crude protein(CP), acid detergent fiber(ADF), and neutral detergent fiber(NDF) did not show the difference. The content of $NO_3-N$ in infiltration water was not more than CF by the 55 150 application, but more than by SS 200 and 55 100+CF 50 treatment.

• Journal of The Korean Society of Grassland and Forage Science
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• v.3 no.2
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• pp.67-76
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• 1983

This experiment was carried out to investigate the effects of the different nitrogen rates and cutting heights on the dead stubble after cutting, and the carbohydrate reserved in stubble of sorghum-sudangrass hybrid (Sorghum bicolor (L.) Moench) Pioneer 988. The experiment was undertaken in the Experimental Livestock Farm of Agriculture Coll., Seoul Nat'l Univ. In Suweon, 1981 and 1982. The results obtained are summarized as follows: 1. The dead stubble after cutting was found to be great with high rate of nitrogen fertilizer and low cutting height. It was also learned that the rainfall during forage cutting period made the dead stubble increased. 2. A significant higher (p<0.05) carbohydrate reserves in the stubble was observed in the high stubble height at the first cutting time and 6th through 7th day after the first cutting. The results indicate that the high stubble height reserves more carbohydrate for the early regrwoth stage after the first cutting when comparing with the low stubble. 3. The content of carbohydrate reserves was influenced by climete. Drought is caused to high content of carbohydrate, whereas, rain is caused to low content of that. The critical soluble carbohydrate content causing the death of stubble supposed to be 3 to 6% at least. 4. It is suggest that carbohydrate reserves in plant do not play a distinctive role for the regrowth in a summer annual forage like sorghum-sudangrass hybrid, but it might be rather influenced by the other factors, for example, environmental conditions at harvest and new bud.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.38 no.5
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• pp.413-417
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• 1993

This study was conducted to breed various cytoplasmic genetic male sterile (CGMS) and restorer lines with backgrounds of Korean japonica rice varieties. The CGMS line BT-CMS was crossed with the restorer line AR-3. The fertile $F_1 was emasculated and crossed with the Korean japonica rice varieties of the early maturing Sobaegbyeo, Odaebyeo, Gwanagbyeo and Daeseongbyeo, and of the medium maturing Hwajinbyeo, Paldal, Suwon 224 and Iri 386, and of the late maturing Nagdongbyeo, Palkweng, Hwacheongbyeo and Milyang 97. Each of the three way cross $F_{1S} was segregated into fertile and sterile individuals. The sterile individuals in each cross were discarded and the fertile individuals were emasculated and backcrossed with the Korean japonica rice varieties. The same process was applied from BC$_1$F$_1$ to BC$_4$F$_1$ generation. In the ${BC_4}{F_1}of each cross, the male sterile individual was crossed with the recurrent Korean japonica variety which was maintainer of male sterility. The male sterile lines of ${BC_6}{F_1}were named as Sobaegbyeo R, Odaebyeo R, Gwanagbyeo R, etc. The fertile individuals homozygous in pollen fertility were selected from the ${BC_4}{F_2}generation and named as Sobaegbyeo R, Odaebyeo R, Gwanagbyeo R, etc. Agronomic characteristics of the CGMS, restorer lines with backgrounds of Korean japonica, and the recurrent Korean japonica rice varieties grown in the field condition were compared. Culm length of the CGMS lines tended to be shorter than that of the recurrent parent, however no significant differences in heading date, panicle length and yield component were found among the CGMS, restorer and recurrent lines.

• Horticultural Science & Technology
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• v.30 no.3
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• pp.329-333
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• 2012

A new Asiatic hybrid lily 'Redsky' cultivar was selected from a cross between 'Vivaldi' cultivar with pale pink color flower as mother plant and 'Prato' with orange colored flower in 2000 year. The selected bulb line was propagated and cultivated for line nomenclature as 'Gonong-F-na-10'. The survey of the growth and flower characteristics of 'Gonong-F-na-10' were conducted from 2005 to 2007 on summer cropping system under structure in Highland Agriculture Research Center, NICS, RDA. After all 'Gonong-F-na-10' was named as 'Redsky'. The flowers of the 'Redsky' cultivar had not soft hair and fragrance, and had orange red (O-R N30D) colored petals with dark brown spots in center of flower. The stem color was dark pink and brown caused by anthocyanin pigment, the stigma and pollen colors were brown. Flowering direction was upward. The flowering date was July 7 so early flowering. The plant height was 97.2 cm so long, number of flowers was 4.9 ea, and length of flower bud was 9.3 cm. The bulb weight was about 34.8 g and its bulb size was about 14.8 cm in circumference. 'Redsky' cultivar was registered as a new variety with No. 3119 in Korea Seed and Variety Service on June of 2010.

• Journal of Science and Technology Studies
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• v.15 no.1
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• pp.145-180
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• 2015

Human Genome Project was a big science done by United States, U.K., France, China, Germany and Japan. But in Korea HGP was not constructed because of lack of governmental funding and failure to attract relevant actors' attention in spite of small voices from early genome researchers and some family members of patients with incurable diseases. This article does not argue that HGP in Korea was an undone science, a concept claimed by Scott Frickel, et al. Instead, it shows the historical fact that HGP was not constructed in Korea in 1990s and analyzes how genomic researches could become possible in Korea in the post-genomic age using the framework of triple-helix. In Korea, researchers have constructed hybrid networks and organizations that intermingles laboratories of university, industry, and government to conduct genomic researches which requires a lot of financial funding. This structure is different from the entrepreneurial university seen in developed countries such as the United States. Using two examples, this article shows that founding a start-up company by university researchers was not an option as in the United States, but a necessity in order to obtain enough funding to conduct genomic researches in Korea. Otherwise, researchers in Korean universities had to form hybrid networks with government to obtain small amount of funds to conduct researches. I argue that this phenomenon shows multifaceted characteristics of institutional structures regarding genomic researches in Korea.

• Journal of The Korean Society of Grassland and Forage Science
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• v.34 no.2
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• pp.81-86
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• 2014

This study was conducted to evaluate the effect of different seeding dates on agronomic characteristics, forage quality, dry matter (DM) and dry matter digestible (DDM) yields of sudangrass hybrid (Sorghum bicolor(L.) Moench) and oat (Avena sativaL.) at Seongju in Kyeongbuk from 2012 to 2013. The experiment was arranged in randomized complete block design with three replications. Sudangrass was seeded five times seeding dates of 10 days interval ranging from $1^{th}$ August to $10^{th}$ September in 2012 and 2013. Oat was seeded three times seeding dates of 10 days interval ranging from $20^{th}$ August to $10^{th}$ September in 2013. Sudangrass was harvested on $26^{th}$ October, 2012 and $2^{nd}$ November, 2013. Plant length and DM content decreased with delaying seeing date. The DM and DDM yields of sudangrass were higher (P<0.05) in first seeding date than all other seeding dates and decreased with delaying seeding dates (P<0.05). The DM and DDM yields of oat were higher (P<0.05) in first and second seeding dates than third seeding date. The results of this study indicated that early seeding of sudangrass would be a recommended than late seeding after $20^{th}$ of August, and late seeding of oat after $30^{th}$ August would be recommened for DM and DDM yields in the Southern Korea.

• Journal of The Korean Society of Grassland and Forage Science
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• v.37 no.2
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• pp.168-175
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• 2017

Single nucleotide polymorphisms (SNP) markers allow rapid screening of crop varieties in early growth stages. We developed a modified SNP PCR procedure for assaying SNPs in maize. For SNP marker development, we chosen 200 SNP sites from MaizeGDB database, and designed two base pair mismatch primers based on putative SNP site of B73 genome sequence. PCR products size was from 200 to 500 bp or was not shown in the case of SNP site existing in Korean silage corns. Using previously discovered 16 primer sets, we investigated distinctness of 50 silage F1 hybrid corns including 10 Korean silage corns developed by RDA such as Gangdaok, Kwangpyeongok, Dapyeongok, Andaok, Yanganok, Singwangok, Jangdaok, Cheongdaok, Pyeonggangok, and Pyeonganok as well as 40 foreign commercial silage corns. From cluster analysis, we confirmed that 10 Korean silage F1 hybrid corns were clearly distinguished except for Singwangok, P1395, and several foreign commercial corns, and selected minimum SNP primer combination for Gangdaok, Jangdaok, Pyeonggangok, and Pyeonganok. Therefore, development of SNP marker sets might be faster, cheaper, and feasible breed discrimination method through simple PCR and agarose gel electrophoresis.