• Journal of Periodontal and Implant Science
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• 제34권1호
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• pp.163-175
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• 2004

This study was performed to evaluate bone formation in the calvaria of rabbit by the concept of guided bone regeneration with titanium mesh membrane and demineralized freeze-dried bone. The animal was sacrificed at 2 weeks, 4 weeks, 8 weeks, and 12 weeks after the surgery. Non-decalcified specimens were processed for histologic analysis. 1. The titanium mesh but the biocompatibility was excellent the cell-occlusiveness was feeble. 2. The cell-occlusiveness was feeble and also the soft tissue growth of the upper part of the newly-formed bone after operating was excellent in early stage. 3. The maintenance ability of the space for the GBR very was excellent. 4. The titanium mesh the tissue-integration was superior the wound fixation ability excellent. 5. The demineralized freeze-dried bone did not promote the bone regeneration. 6. With the lapse of time, formation quantity of the bone some it increased, it increased quantity very it was feeble. Within the above results, the titanium mesh for the guided bone regeneration was excellent, the dεmineralized freeze-dried bone confirmed does not promote bone regeneration.

• 한국동물위생학회지
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• 제33권1호
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• pp.1-6
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• 2010

Monoclonal antibody (mAb)-based enzyme immune-slide assay (EISA) was used for the detection of Peste des petits ruminants (PPR) virus from field samples collected from a natural outbreak. The clinicopathological study was undertaken to diagnose the case primarily of PPR. Antigen was detected from discharges and faeces of infected goats and swabs of postmortem lesions prepared on glass slide or glass plate using acetone fixation. Nasal discharge collected at the early stage of disease course or lung is an appropriate ante- or postmortem sample for this technique, respectively. Convalescent polyclonal sera collected from recovered animals which were diagnosed as PPR by EISA showed high antibody titer against PPR by C-ELISA, demonstrating the satisfactory specificity of the test. Therefore, EISA is a sensitive and specific assay to confirm PPR infection both in field and laboratory conditions and especially suitable for developing country.

• 대한족부족관절학회지
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• 제7권2호
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• pp.157-165
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• 2003

Purpose: The purpose of this study was to the evaluate the clinical and radiographic results and complication of the Scarf osteotomy which is one of the surgical treatment of Hallux valgus. Material and Method: Fifty feet (30 patients) of moderate to severe hallux valgus deformity were operated by Scarf osteotomy procedure from January 2002 to July 2002. Patients were asked about their satisfaction in terms of pain, appearance and motion. The AOFAS clinical ratio scales and radiographic results for hallux valgus were evaluated. Result: The mean Hallux valgus angle improved from an average of 30.6 degrees to 9.0 degrees and the first intermetatarsal angle improved from an average 13.3 degrees to 5.2 degrees respectively. The preoperative mean AOFAS score was 58 points and on last follow up AOFAS score improved to 88 points. Complication which including troughing, nonunion, malunion was not developed. Conclusion: We found that Scarf osteotomy is a reliable operation for mild to moderate Hallux valgus deformity as regards firm fixation and early postoperative ambulation and large contact area for union.

• Journal of Korean Neurosurgical Society
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• 제30권7호
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• pp.870-875
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• 2001

Objectives : Anterior tunnelling technique consist of anterior cervical fractional interspace decompression without fusion. This method provides sufficient space for adequate neuroforaminal decompression but avoids the need for fusion or fixation. We report early clinical results of 32 cases that underwent anterior tunnelling operation for treatment of cervical radiculopathy. Methods : This method is identical to conventional approach until the exposure of anterior cervical body and bilateral retraction of longus colli is made. A vertical window is then made at the vertebral bodies and disc space lateral to the insertion site of the longus colli. The window is deepened with drilling that follows a tunnelling fashion down to the compressive lesion. We analyzed clinical results from 32 patients who treated between December 1998 and August 2000. Results : Satisfactory results were obtained in 87% of the patients. Two patients required revision surgery. None revealed surgical spinal instability on last follow-up. Conclusion : Anterior tunnelling operation is an acceptable surgical option for the treatment of cervical radiculopathy. Its advantages are short hospitalization, minimal postoperative discomfort, and technical feasibility.

• 대한정형외과학회지
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• 제56권6호
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• pp.530-534
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• 2021

소아, 특히 유아의 치상돌기 골절은 매우 드물고, 따라서 그 진단 및 치료에 대하여 정립된 기술을 쉽게 볼 수 없다. 특히 전위가 심한 경우 보존적 혹은 수술적 치료 중 무엇을 적용해야 할지 결정하기가 어렵다. 이론적으로 수술적 고정을 통해 골절의 조기 정복과 견고한 유지가 가능할 것으로 보이나, 작고 연약한 소아에서 안전하게 수행되기가 쉽지 않고, 환축추 관절의 영구적 고정이라는 문제가 남는다. 저자들은 교통사고로 발생한 2세 여아의 고도 굴곡 전위된 치상돌기 골절 1예를 보존적으로 치료하여 양호한 결과를 얻었기에 이 체험을 문헌 고찰과 함께 보고하는 바이다.

• Hip & pelvis
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• 제34권2호
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• pp.79-86
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• 2022

Purpose: The purpose of this study was to determine whether the direct anterior approach (DAA) can be applied to hemiarthroplasty for treatment of unstable femoral intertrochanteric fractures in elderly patients. Materials and Methods: We conducted a retrospective review of 34 cases (34 patients) who underwent bipolar hemiarthroplasty using a DAA from February 2019 to April 2020. The mean age of the patients was 82.68 years (range, 67-95 years). A cementless distal fixation hip system was used in all cases. Operation time, total amount of blood loss, intraoperative and postoperative fractures, infections, and reoperations were measured. The patients performed weight-bearing walking as tolerated immediately after surgery. Measurement of migration of the greater trochanter (GT) and progressive subsidence of the femoral stem was performed using follow-up radiographs. Results: The mean operative time was 83.50 minutes (range, 60-120 minutes). The mean amount of bleeding was 263.53 mL (range, 112-464 mL). Walking started a mean of 3.03 days (range, 3-4 days) after surgery. There was no case of progressive migration of the GT more than 5 mm even though it was not fixed. There were no cases of infection, dislocation, or reoperation. Conclusion: Bipolar hemiarthroplasty using the DAA for treatment of unstable femoral intertrochanteric fractures could be considered a useful option that provides many advantages in elderly patients.

• Journal of Plant Biology
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• 제37권3호
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• pp.387-394
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• 1994

보리수나무(Elaeagus umbellata Thunb.) 유식물의 생육과정에서 근류 형성 및 질소고정 활성에 대한 중요 요인으로서 광도, 온도, 질소화합물, 수분 스트레스 등의 영향을 정량적으로 분석하였다. 유식물의 상대생장률과 T/R비는 각각 초기의 $1.60%{\cdot}d^{-1}$와 1.12에서 근류 형성이 촉진된 후기에는 $3.75%{\cdot}d^{-1}$와 2.31로 증가하였다. 질소고정의 비활성은 근류 형성 초기에 $72.5\;\mu\textrm{M}\;C_2H_4{\cdot}g\;fr\;wt\;nodule^{-1}{\cdot}h^{-1}$로서 최대치를 나타낸 후 점차 감소하였다. 상대조도 20-25. 10-15 및 4-6% 처리구의 질소고정 활성은 대조구에 대해 각각 41, 54 및 71%의 감소를 보였다. 근류를 15, 20, 25 및 $30^{\circ}C$에서 배양할 때 활성은 각각 5.4, 24.7, 51.6 및 $58.5\;\mu\textrm{M}\;C_2H_4{\cdot}g\;fr\;wt\;nodule^{-1}{\cdot}h^{-1}$로 변하였다. 저온($15^{\circ}C$)의 전처리 후 $30^{\circ}C$로 옮겼을 때 근류 활성($66.5\;\mu\textrm{M}\;C_2H_4{\cdot}g\;fr\;wt\;nodule^{-1}{\cdot}h^{-1}$)은 고온($35^{\circ}C$) 전처리에 비해 촉진되었다. 근류의 활성은 산소분압에 비례하여 16 kPa 이상에서 포화되었고, 근류를 뿌리에서 떼어내거나 절단하면 92%와 43%로 활성이 억제되었다. pH 5.5, 6.5 및 8.0에서 생육된 초기 근류의 상대활성은 각각 89, 100 및 40%였으며, 1 및 3 mM의 $NO_3\;-N와\;{NH_4}^{+}-N$에서 생육된 초기 근류는 각각 6.1%와 68.50%의 상대활성을 보였다. 수분 공급량 20, 50 및 100mL에 의한 근류의 형성과 비활성은 각각 35, 120 및 8 mg.plant-1와 33.6, 58.4 및 $8.4\;\mu\textrm{M}\;C_2H_4{\cdot}g\;fr\;wt\;nodule^{-1}{\cdot}h^{-1}$였다. 식물체에 수분공급을 중단하였을 때 5일 후에 일시 위조가 나타났으며, 식물체의 함수량 및 증산량은 각각 90 및 53% 감소되었으나, 근류 활성은 완전히 소실되었다.

• Journal of Yeungnam Medical Science
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• 제20권1호
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• pp.28-35
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• 2003

대퇴골두 골단분리증으로 진단받고 수술적 치료를 받았던 14례(17 고관절)을 대상으로 이들을 1개의 나사못을 이용한 정위치고정술을 시행한 환자군(1군)과 대퇴경부 절골술 및 다발성 핀 삽입술을 함께 시행한 환자군(2군)으로 나누어 골단-간부각을 측정하여 수치를 술전, 술 후 및 마지막 추시를 비교하여 금속 내 고정술 시행 후 골단판 재형성을 확인하여 다음과 같은 결과를 얻었다. 총 14명(17 고관절) 중 6명(7 고관절)에서 골단판 재형성을 확인 할 수 있었다. 정위치고정술을 시행한 환자 11명(13례) 중, 4명(4 고관절, 평균 $7.75^{\circ}$)에서, 대퇴경부 절골술 및 다발성 핀 삽입술을 함께 시행한 환자 3명(4례) 중 2명(3 고관절, 평균 $10.7^{\circ}$)에서 골단판 재형성을 확인할 수 있었다. 골단판 재형성이 확인된 7례 중 경도의 분리가 3례(1군 3례), 중등도의 분리가 4례(1군 1례, 2군 3례)로 골단판 재형성의 빈도는 분리의 정도가 심할수록 증가되는 소견을 보였고 발병 당시 나이와는 연관성이 없었다. 골단판 재형성이 확인된 7례 중 6례에서 골단판 조기 폐쇄를 확인하였다.

• 대한치과보철학회지
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• 제43권6호
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• pp.751-763
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• 2005

Statement of problem. To improve a direct implant fixation to the bone, various strategies have been developed focusing on the surface of materials. The surface quality of the implant depends on the chemical, physical, mechanical and topographical properties of the surface. The different properties will interact with each other and a change in thickness of the oxide layer may also result in a change in surface energy, the surface topography and surface, chemical composition. However, there is limited the comprehensive study with regard to changed surface and biologic behavior of osteoblast by anodization. Purpose of study. The aim of this study was to analyze the characteristics of an oxide layer formed and to evaluate the cellular biologic behaviors on titanium by anodic oxidation (anodization) by cellular proliferation, differentiation, ECM formation and gene expression. And the phospholipase activity was measured on the anodized surface as preliminary study to understand how surface properties of Ti implant are transduced into downstream cellular events. Methods and Materials. The surface of a commercially pure titanium(Grade 2) was modified by anodic oxidation. The group 1 samples had a machined surface and other three experimental specimens were anodized under a constant voltage of 270 V(Group 2), 350 V(Group 3), and 450 V(Group 4). The specimen characteristics were inspected using the following five categories; the surface morphology, the surface roughness, the thickness of oxide layer, the crystallinity, and the chemical composition of the oxide layer. Cell numbers were taken as a marker for cell proliferation. While the expression of alkaline phosphatase and Runx2 (Cbfa1) was used as early differentiation marker for osteoblast. The type I collagen production was determined, which constitutes the main structural protein of the extracellular matrix. Phospholipase $A_2$ and D activity were detected. Results. (1) The anodized titanium had a porous oxide layer, and there was increase in both the size and number of pores with increasing anodizing voltage. (2) With increasing voltage, the surface roughness and thickness of the oxide film increased significantly (p<0.01), the $TiO_2$phase changed from anatase to rutile. During the anodic oxidization, Ca and P ions were more incorporated into the oxide layer. (3) The in vitro cell responses of the specimen were also dependant on the oxidation conditions. With increasing voltage, the ALP activity, type I collagen production, and Cbfa 1 gene expression increased significantly (p<0.01), while the cell proliferation decreased. (4) In preliminary study on the relation of surface property and phospholipase, PLD activity was increased but $PLA_2$ activity did not changed according to applied voltage. Conclusion. The anodized titanium shows improved surface characteristics than the machined titanium. The surface properties acquired by anodization appear to give rise more mature osteoblast characteristics and might result in increased bone growth, and contribute to the achievement of a tight fixation. The precise mechanism of surface property signaling is not known, may be related to phospholipase D.

• 한국수정란이식학회지
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• 제17권3호
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• pp.211-218
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• 2002

본 연구는 체외에서 소 난포란유래의 배반포 생산에 있어서 배지 내에 첨가하는 외인성 고정질소 원으로써 아미노산과 FBS의 첨가효과를 검토하였다. 소 난포란의 체외성숙은 TCM-l99용액, 체외 수정은 Fer-TALP용액으로 행하였으며, 체외수정후 24시간째(day 1)의 수정난자를 체외배양에 제공하였다. 체외배양용 기초배지는 YS용액, 기초 배양법은 25개 난자/10 ${\mu}\ell$ 배지의 단순.미소적배양법을 이용하였다. 본 연구의 결과를 요약하면 다음과 같다. 1. 체외 수정란의 배양에 있어서 비필수 아미노산(MEM 유래) 첨가가 무첨가군보다 높은 배반포 발달율을 나타냈다. 2. 체외 수정란의 배양에 있어서 필수 아미노산(RPMI 1640 유래) 첨가가 무첨가군보다 유의하게 높은 배반포 발달율을 나타냈다. (P<0.05) 3. Day 1에 비 필수 아미노산, day 5에 필수 아미노산을 첨가하였을 경우 부화 배반포로의 발생율 및 배반포로의 부화율을 향상시켰다. 4. Day 1에 비필수.필수 아미노산을 첨가한 후 day 3, day 4, day 5에 각각 필수 아미노산만을 배지로부터 제거 시 배반포의 부화율은 현저하게 낮았다. 5. FBS의 첨가시기는 배양 후기(day 5)에 첨가할 수록 배반포율 또는 부화 배반포율이 유의하게 상승하였다(p<0.05). 이상의 결과에서 소 난포란 유래 배반포의 체외생산에 있어서 배지에 첨가하는 외인성 고정 질소 원들의 첨가에 있어서 첨가시기 및 농도를 조절함으로써 양질의 배반포 생산을 향상시킬 수 있는 것으로 사료된다.