• Proceedings of the Korea Society of Environmental Biology Conference
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• 2002.11a
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• pp.127-131
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• 2002

The OJIP transients were investigated from leaves of Crinum asiaticum var. japonicum, Osmanthus insularis, Chloranthus glaber and Asplenium antiquum, in order to screen the indicators for quantification of environmental stresses and to apply them to the plant vitality test. The obvious diurnal changes or OJIP transients were found in Crinum asiaticum var. japonicum, Osmanthus insularis growing in sunny habitats, showing lower Fp and higher Fo in day time. The values of SF $I_{no}$ and P $I_{NO}$ increased dramatically in day time in leaves of Crinum asiaticum var. japonicum, Osmanthus insularis, and Asplenium antiquum. Although the value of RC/CS decreased slightly in day time in leaves of 4 tropica $l_tropical plants, the obvious diurnal changes or OJIP transients were not seen in winter, suggesting that tropica $l_tropical paints used in this research seemed to be irreversibly damaged in winter. However, the values of ETo/RC and Ψo/(1-Ψo) increased significantly in leaves of Crinum asiaticum var. japonicum, indicating that ETO/RC and Ψo/(1-Ψo) were useful as indicator parameters for scensing of low temperature.ature.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.10a
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• pp.57-57
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• 2019

실내 수준에서 조사되는 광량조건에 따른 자생 상록활엽 2종의 생육과 광화학반응을 조사하였다. 식물재료는 3년생 사철나무(Euonymus japonicus Thunb.) 및 2년생 돈나무[Pittosporum tobira (Thunb.) W. T. Aiton] 실생묘로 유리온실에서 재배하면서 실험에 사용하였다. 실내 광량은 10, 50, 100 및 200 PPFD(${\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$)로 달리하여 8주간 재배하였으며, 광주기(12/12 h), 온도($25{\pm}1^{\circ}C$), 습도($55{\pm}3%$) 및 관수(1회/3일)조건은 고정되었다. 생육특성 및 광화학반응의 요인들이 측정되었으며, 동일기간 동안 유리온실에서 재배된 식물을 대조구로 사용하였다. 실험의 결과, 사철나무는 100, 200 PPFD의 광량에서 대조구에 비해 초장, 줄기직경, 엽수 및 엽장의 생육반응이 우수하였으며, 엽록소함량은 100 PPFD 이상의 광량에서 감소하였다. 또한 100 PPFD 이상의 광량에서 스트레스지수(Fm/Fo), 최대양자수율(Fv/Fm) 및 전자전달효율(ETo/RC)이 감소하였으며, 이는 대조구와도 유사한 경향이었다. 돈나무는 모든 광량조건에서 줄기직경과 엽폭을 제외하고 대조구와 관계없이 생육반응이 일정하였다. 그러나 스트레스지수와 최대양자수율은 10 PPFD 처리구에서 가장 낮게 조사되었고, 비광학적 에너지의 손실(DIo/RC)은 2.53으로 가장 높았다.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2020.08a
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• pp.55-55
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• 2020

This study investigated the growth and chlorophyll fluorescence reactions of three Ardisia genus grown under various indoor light intensity conditions with the aim of evaluating their suitability as indoor plants. Young seedlings of A. crispa (Thunb.) A.DC., A. pusilla DC., and A. japonica (Thunb.) Blume were used in the experiment. The plants were cultivated indoors for 10 weeks under different light intensities: 10, 50, 100, and 200 PPFD (μmol·m^-2·s^-1), and their growth was compared with that of plants cultivated in a greenhouse during the same period (mean value 236.8±20.4 PPFD at noon). Also, chlorophyll fluorescence analysis was investigated with a portable PAM fluorometer. The indoor plants were maintained at 12/12 h photoperiod, temperature at 25±1℃, and humidity at 55±3%. Irrigation frequency (once every three days) was the same for the indoors and the greenhouse. The results of growth in three Ardisia plants showed that almost all parameters except leaf number and chlorophyll content had similar levels regardless of light intensity. A. crispa and A. pusilla plants grown in 200 PPFD were investigated to have low chlorophyll contents. Meanwhile, chlorophyll fluorescence parameters differed based on light levels. In A. crispa, the Fv/Fm (0.77), DIo/RC (0.47) and Fm/Fo (4.77) parameters tended to be poor at 200 PPFD compared to those at other light intensities. Similarly, the DIo/RC, Fm/Fo, and Pi_Abs parameters of A. pusilla plant (200 PPFD) are 0.45, 4.48 and 2.42, respectively, which can be considered stress. The analysis of fluorescence in A. japonica showed that all parameters except ETo/RC had similar levels regardless of light intensity. The ETo/RC parameter was 0.49 and 0.72 in the control plants and plants 200 PPFD, respectively, which was lower than those in plants at other light intensities. Therefore, it seems that the relatively high light intensity acted as a stressor for Ardisia plants.

• Journal of Environmental Science International
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• v.14 no.4
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• pp.389-395
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• 2005

The changes of O-J-I-P transients were investigated using leaves of four subtropical plant species (Crinum asiaticum var. japonicum, Osmanthus insularis, Chloranthus glaber and Asplenium antiquum) under the natural conditions in winter, in order to select the stress indicators for diagnosing physiological states of plants under low temperature. In the O-J-I-P transients of these species, the fluorescence intensity was found to be higher in O-step and lower in P-step in winter than in summer. Particularly, the fluorescence intensity of the P-step in Crinum asiaticum var. japonicum was lower than those of other three plant species, indicating that Crinum asiaticum var. japonicum is the most sensitive to low temperature. Of the chlorophyll fluorescence parameters derived from O-J-I-P transients of four subtropical plants, Fm, Fv/Fo, ABS/CS, TRo/CS, $\phi_{po}$ and $\phi_{po}/(1-\phi_{po})$ decreased significantly with the increase of Fo, Sm, N, EToICS, ETo/RC and $\psi_o/(1-\psi_o)$ depending on temperature drop in winter. Therefore, these parameters could be used as indicators for estimating low temperature stress and diagnosing physiological states of plants under the natural conditions in winter.

• Horticultural Science & Technology
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• v.32 no.3
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• pp.318-329
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• 2014

In order to gain insight into the physiological responses of plants to high temperature stress, the effects of temperature on Chinese cabbage (Brassica campestris subsp. napus var. pekinensis cv. Detong) were investigated through analyses of photosynthesis and chlorophyll fluorescence under 3 different temperatures in the temperature gradient tunnel. Growth (leaf length and number of leaves) during the rosette stage was greater at ambient $+4^{\circ}C$ and ambient $+7^{\circ}C$ temperatures than at ambient temperature. Photosynthetic $CO_2$ fixation rates of Chinese cabbage grown under the different temperatures did not differ significantly. However, dark respiration rate was significantly higher in the cabbage that developed under ambient temperature relative to elevated temperature. Furthermore, elevated growth temperature increased transpiration rate and stomatal conductance resulting in an overall decrease of water use efficiency. The chlorophyll a fluorescence transient was also considerably affected by high temperature stress; the fluorescence yield $F_J$, $F_I$, and $F_P$ decreased considerably at ambient $+4^{\circ}C$ and ambient $+7^{\circ}C$ temperatures, with induction of $F_K$ and decrease of $F_V/F_O$. The values of RC/CS, ABS/CS, TRo/CS, and ETo/CS decreased considerably, while DIo/CS increased with increased growth temperature. The symptoms of soft-rot disease were observed in the inner part of the cabbage heads after 7, 9, and/or 10 weeks of cultivation at ambient $+4^{\circ}C$ and ambient $+7^{\circ}C$ temperatures, but not in the cabbage heads growing at ambient temperature. These results show that Chinese cabbage could be negatively affected by high temperature under a future climate change scenario. Therefore, to maintain the high productivity and quality of Chinese cabbage, it may be necessary to develop new high temperature tolerant cultivars or to markedly improve cropping systems. In addition, it would be possible to use the non-invasive fluorescence parameters $F_O$, $F_V/F_M$, and $F_V/F_O$, as well as $F_K$, $M_O$, $S_M$, RC/CS, ETo/CS, $PI_{abs}$, and $SFI_{abs}$ (which were selected in this study), to quantitatively determine the physiological status of plants in response to high temperature stresses.

• Korean Journal of Environmental Biology
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• v.30 no.2
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• pp.98-106
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• 2012

The chlorophyll fluorescence and photosynthetic $CO_2$ fixation capacity of leaves from three major crop trees found on Jeju Island, Camellia sinensis L., Camellia japonica L., and Citrus unshiu M., were analyzed. The photosynthetic $CO_2$ fixation rate of C. sinensis was similar to that of C. unshiu, and much higher than that of C. japonica which belongs to the same genus. Stomatal conductance in the three species was high at dawn and low during daytime. The intercellular $CO_2$ concentration of the three species was also high at dawn and decreased at midday. The transpiration rate showed an opposite trend from the intercellular $CO_2$ concentration. The photochemical efficiencies of PSII (Fv/Fm) in C. sinensis were slightly lower at midday compared to the level at dawn and/or dusk. The decline in Fv/Fm of C. sinensis at midday was much smaller than that of C. japonica. These results indicate that C. sinensis is better acclimated to high levels of radiation under natural conditions in late summer, although its PSII reaction center was inhibited by strong radiation. Of the chlorophyll fluorescence parameters in the species, the RC/CS decreased significantly while the ABS/RC, TRo/RC, ETo/RC, and DIo/RC increased significantly at midday in late summer. However, C. unshiu did not show significant changes in these values depending on the time of day. Among the three species, the daily $CO_2$ fixation rate in C. sinensis ($320.1mmol\;m^{-2}d^{-1}$) was the highest, followed by that of C. unshiu ($292.5mmol\;m^{-2}d^{-1}$) and C. japonica ($244.8mmol\;m^{-2}d^{-1}$). Thus, C. sinensis may be a valuable crop tree in terms of the uptake of $CO_2$ under natural field conditions.

• Journal of Environmental Science International
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• v.22 no.6
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• pp.705-715
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• 2013

The response of the freshwater microalga Chlorella vulgaris to mercuric ion ($Hg^{2+}$) stress was examined using chlorophyll a fluorescence image analysis and O-J-I-P analysis as a way to monitor the toxic effects of mercury on water ecosystems. The levels of photosynthetic pigments, such as chlorophyll a and b and carotenoids, decreased with increasing $Hg^{2+}$ concentration. The maximum photochemical efficiency of photosystem II(Fv/Fm) changed remarkably with increasing $Hg^{2+}$ concentration and treatment time. In particular, above $200{\mu}M\;Hg^{2+}$, considerable mercury toxicity was seen within 2 h. The chlorophyll a fluorescence transient O-J-I-P was also remarkably affected by $Hg^{2+}$; the fluorescence emission decreased considerably in steps J, I, and P with an increase in $Hg^{2+}$ concentration when treated for 4 h. Subsequently, the JIP-test parameters (Fm, Fv/Fo, RC/CS, TRo/CS, ETo/CS, ${\Phi}_{PO}$, ${\Psi}_O$ and ${\Phi}_{EO}$) decreased with increasing $Hg^{2+}$ concentration, while N, Sm, ABS/RC, DIo/RC and DIo/CS increased. Therefore, a useful biomarker for investigating mercury stress in water ecosystems, and the parameters Fm, ${\Phi}_{PO}$, ${\Psi}_O$, and RC/CS can be used to monitor the environmental stress in water ecosystems quantitatively.

• Journal of Environmental Science International
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• v.18 no.12
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• pp.1361-1368
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• 2009

The effects of $Cd^{2+}$ ions on the Chl a fluorescence of 5 hydrophytes (e.g. Lemna, Salvinia, Ricciocarp, Nymph, Typha plants) were investigated in order to select $Cd^{2+}$-sensitive plant species and to get informations on physiological responses of plants to $Cd^{2+}$ stress. Lemna plants were most sensitive to cadmium stress, while Nymph plants were tolerant. However, in all $Cd^{2+}$-treated plants, Fv/Fm, the maximum photochemical efficiency of PS II, decreased in proportion to the increase of $Cd^{2+}$ concentration and treatment time. The Chl a fluorescence transient O-J-I-P was also considerably affected by $Cd^{2+}$ ions; the fluorescence yield decreased consid- erably in steps J, I and P in $Cd^{2+}$ treated plants, although it followed a typical polyphasic rise in non-treated plants. In Lemna plants, the functional parameters, ABS/CS, TRo/CS와 ETo/CS and RC/CS, decreased in proportion to the increase of $Cd^{2+}$ concentration, while N, Mo and Kn increased. The structural parameters, ${\Phi}po$, ${\Phi}po$/($1-{\Phi}po$), Plabs, SFlabs, Kp and RC/ABS, also decreased according to the increase of $Cd^{2+}$ concentration. Consequently, Lemna plants will be useful as a experimental model system to investigate responses of plants. And several functional or structural parameters could be applied to determine quantitatively the physiological states of plants under stresses.

• The Plant Pathology Journal
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• v.35 no.2
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• pp.112-124
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• 2019

Barley worldwide is affected seriously by Fusarium seedling blight (FSB) and Fusarium head blight (FHB) diseases caused by the Fusarium species. The objective of this study was to facilitate the resistance of hulled and hull-less barley at different growth stages to F. culmorum according to direct parameters: disease rating (DR), fresh weight of leaves and roots, kernel weight per spike, kernel number per spike, plump kernels, and indirect parameters - chlorophyll a fluorescence (CF). Plate assay, greenhouse and field tests were performed on 30 spring barley doubled haploid (DH) lines and their parents infected with Fusarium culmorum. Direct parameters proved that hulled genotypes show less symptoms. Most studied chlorophyll a fluorescence (CF) parameters (apart from DIo/CS - amount of energy dissipated from PSII for laboratory test, TRo/CS - amount of excitation energy trapped in PSII reaction centers, ETo/CS - amount of energy used for electron transport and RC/CS - number of active reaction centres in the state of fully reduced PSII reaction center in field experiment) were significantly affected by F. culmorum infection. In all experiments, hulled genotypes had higher values of CF parameters compared to hull-less ones. Significant correlations were detected between direct and indirect parameters and also between various environments. It was revealed that ABS/CS, TRo/CS, and RC/CS have significant positive correlation in greenhouse test and field experiment. Significant correlations suggest the possibility of applying the CF parameters in selection of barley DH lines resistant to F. culmorum infection.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2020.08a
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• pp.67-67
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• 2020

Though there is an on-going need for the diversity of ornamental plants for in-door environment, their growth and stress adaptability in comparatively low light intensity condition require further studies for implementation. Here investigates the growth and chlorophyll fluorescence parameters of Farfugium japonicum (L.) Kitam, Liriope muscari (Decne.) L.H.Bailey and Acorus gramineus Aiton under several light intensities which were based on common in-door environment. The growth measurement of the plants included the quantity, length, width and SPAD value of the leaves. Calculated values of Fm/Fo, Fv/Fm, Pi_Abs, ETo/RC and DIo/RC were used as the parameters of the chlorophyll fluorescence under 10, 50, 100 and 200 PPFD (μmol·m^-2·s^-1) light intensities. In-door plants group was put in a closed room allowing no other light sources for 10 weeks and the control group was put in glass-greenhouse for the same period. The overall in-door growth of L. muscari was not significantly different in all light intensities compared to the control group and even showed the higher SPAD values. Also, an increasing tendency of Pi_Abs value under 10 to 100 PPFD was observed implying that L.muscari could adapt well to in-door environment. Measurement of A. gramineus growth mostly showed the highest values in the control group especially in the number of the leaves. Nevertheless, chlorophyll fluorescence parameters showed no significant value difference between in-door and the control groups and thus, A. gramineus might have possibility of successful adaptation to in-door environment. F. japonicum showed deficient growth in plant height and leaf length compared to the control but, it seemed to be able to sustain ornamental value under in-door light intensities. Furthermore, Pi_Abs and DIo/RC values were increased under in-door light conditions suggesting potential adaptability of F. japonicum.