• Journal of Embryo Transfer
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• v.20 no.2
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• pp.123-128
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• 2005

This study examines the effects of kinds and concentrations of cryoprotectants on the survival rate of vitrification-thawed porcine oocytes, together with the effects on survival, in vitro fertilization and development of immature oocytes. 1. The developmental rate of oocytes to MII and diploid stage when the vitrification-thawed of recovered immature oocytes cultured for 0, 15, 30 and 40h were cultured for 0, 15, 30 and 40h were $56.7\%,\;53.3\%,\;63.3\%,\;65.0\%\;and\;23.3\%,\;18.3\%,\;10.0\%,\; 3.3\%$, respectively. The in vitro development to MII stage were lower than the control group $(78.2\%)$, but higher fo. diploid stage $(5.5\%)$. 2. When the vitrification of immature oocytes after being culture for 0, 15, 30 and 40 hours, the survival rate were $34.0\%,\;26.0\%,\;18.0\%\;and\;10.0\%$ respectively. This result was lower than that of the control group $(60.0\%)$. 3. When the fertilization of the vitrified immature oocytes after being culture for 0, 15, 30 and 40 hours, the in vitro fertilization rate were $60.0\%,\;54.0\%,\;48.0\%,\;38.0\%$, and developmental rates were $26.0\%,\;18.0\%,\;8.0\%,\;4.0\%$, respectively. This results were lower than the control group $(78.0\%\;and\;38.0\%)$. 4. When the fertilization of the immature oocytes after being culture for $0\~15$ hours vitrified with EDS and ETS, the fertilization and developmental rates were $50.0\%,\; 22.0\%$ and $46.0\%,\;18.0\%$, respectively. This results were lower than the control group $(74.0\%\;and\;38.0\%)$.

• The Korean Journal of Air & Space Law and Policy
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• v.25 no.1
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• pp.3-26
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• 2010

The European Union(EU) has recently introduced its Directive 2008/101/EC to include aviation in the EU ETS(emissions trading system). As an amendment to Directive 2003/87/EC that regulates reduction of the green house gas(GHG) emissions in Europe in preparation for the Kyoto Protocol, 1997, it obliges both EU and non-EU airline operators to reduce the emission of the carbon dioxide(CO2) significantly in the year 2012 and thereafter from the level they made in 2004 to 2006. Emission allowances allowed free of charge for each airline operator is 97% in the first year 2012 and 95% from 2013 and thereafter from the average annual emissions during historical years 2004 to 2006. Taking into account the rapid growth of air traffic, i.e. 5% in recent years, airlines operating to EU have to reduce their emissions by about 30% in order to meet the requirements of the EU Directive, if not buy the emissions right in the emissions trading market. However, buying quantity is limited to 15% in the year 2012 subject to possible increase from the year 2013. Apart from the hard burden of the airline operators, in particular of those from non-European countries, which is not concern of this paper, the EU Directive has certain legal problems. First, while the Kyoto Protocol of universal application is binding on the Annex I countries of the Climate Change Convention, i.e. developed countries including all Member States of the European Union to reduce GHG at least by 5% in the implementation period from 2008 to 2012 over the 1990 level, non-Annex I countries which are not bound by the Kyoto Protocol see their airlines subjected to aircraft emissions reductions scheme of EU when operating to EU. This is against the provisions of the Kyoto Protocol dealing with the emissions of GHG including CO2, target of the EU Directive. While the Kyoto Protocol mandates ICAO to set up a worldwide scheme for aircraft emissions to contribute to stabilizing GHG concentrations in the atmosphere at a level that would prevent dangerous anthropogenic interference with the climate system, the EU ETS was drawn up outside the framework of the international Civil Aviation Organization(ICAO). Second, EU Directive 2008/101 defines 'aviation activities' as covering 'flights which depart from or arrive in the territory of a Member State to which the [EU] Treaty applies'. While the EU airlines are certainly subject to the EU regulations, obliging non-EU airlines to reduce their emissions even if the emissions are produced during the flight over the high seas and the airspace of the third countries is problematic. The point is whether the EU Directive can be legally applied to extra-territorial behavior of non-EU entities. Third, the EU Directive prescribes 2012 as the first year for implementation. However, the year 2012 is the last year of implementation of the Kyoto Protocol for Annex I countries including members of EU to reduce GHG including the emissions of CO2 coming out from domestic airlines operation. Consequently, EU airlines were already on the reduction scheme of CO2 emissions as long as their domestic operations are concerned from 2008 until the year 2012. But with the implementation of Directive 2008/101 from 2012 for all the airlines, regardless of the status of the country Annex I or not where they are registered, the EU airlines are no longer at the disadvantage compared with the airlines of non-Annex I countries. This unexpected premium for the EU airlines may result in a derogation of the Kyoto Protocol at least for the year 2012. Lastly, as a conclusion, the author shed light briefly on how the Korean aviation authorities are dealing with the EU restrictive measures.

• Korean Journal of Agricultural and Forest Meteorology
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• v.18 no.4
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• pp.307-319
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• 2016

A Land-Atmosphere Modeling Package (LAMP) for supporting agricultural and forest management was developed at the National Center for AgroMeteorology (NCAM). The package is comprised of two components; one is the Weather Research and Forecasting modeling system (WRF) coupled with Noah-Multiparameterization options (Noah-MP) Land Surface Model (LSM) and the other is an offline one-dimensional LSM. The objective of this paper is to briefly describe the two components of the NCAM-LAMP and to evaluate their initial performance. The coupled WRF/Noah-MP system is configured with a parent domain over East Asia and three nested domains with a finest horizontal grid size of 810 m. The innermost domain covers two Gwangneung deciduous and coniferous KoFlux sites (GDK and GCK). The model is integrated for about 8 days with the initial and boundary conditions taken from the National Centers for Environmental Prediction (NCEP) Final Analysis (FNL) data. The verification variables are 2-m air temperature, 10-m wind, 2-m humidity, and surface precipitation for the WRF/Noah-MP coupled system. Skill scores are calculated for each domain and two dynamic vegetation options using the difference between the observed data from the Korea Meteorological Administration (KMA) and the simulated data from the WRF/Noah-MP coupled system. The accuracy of precipitation simulation is examined using a contingency table that is made up of the Probability of Detection (POD) and the Equitable Threat Score (ETS). The standalone LSM simulation is conducted for one year with the original settings and is compared with the KoFlux site observation for net radiation, sensible heat flux, latent heat flux, and soil moisture variables. According to results, the innermost domain (810 m resolution) among all domains showed the minimum root mean square error for 2-m air temperature, 10-m wind, and 2-m humidity. Turning on the dynamic vegetation had a tendency of reducing 10-m wind simulation errors in all domains. The first nested domain (7,290 m resolution) showed the highest precipitation score, but showed little advantage compared with using the dynamic vegetation. On the other hand, the offline one-dimensional Noah-MP LSM simulation captured the site observed pattern and magnitude of radiative fluxes and soil moisture, and it left room for further improvement through supplementing the model input of leaf area index and finding a proper combination of model physics.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.47 no.3
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• pp.205-212
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• 2021

The skin fibroblasts of different origins showed different expression levels of MMP-1 in response to TNF-α treatment or UV irradiation. We hypothesized that this is caused by polymorphism in the MMP-1 promoter region. To elucidate it, first of all, we analyzed and classified the genotype of the -1607 site of the MMP-1 promoter in 23 commercially available primary fibroblasts, and then we examined the expression of MMP-1 by TNF-α or UVB stimulation for each classified genotype. As a result of the analysis, fibroblasts with 6 1G/1G genotypes, 10 1G/2G genotypes, and 7 2G/2G genotypes were identified. Hs68 and Detroit 551 cell lines were confirmed to have 1G/2G genotypes. In the 1G/1G genotype, MMP-1 was expressed twice as high as that of the control group by TNF-α treatment, and was hardly expressed by UV light. In the case of the 1G/2G genotype, MMP-1 was expressed 2.45 fold higher by TNF-α treatment, and 1.4 fold by UV light than the control. In the case of the 2G/2G genotype, MMP-1 was expressed 1.35 fold by TNF-α treatment, and was highly expressed by 2.5 fold by ultraviolet rays compared to control. It can be estimated that MMP-1 expression is better induced in the 1G genotype by TNF-α and in the 2G genotype by UV light. In addition, it can be presumed that MMP-1 expression is increased by creating a site where the Ets transcription factor can bind by another G inserted at the -1607 position. These studies have not been conducted at all in fibroblasts in relation to skin aging, so it is an area that needs to be further studied in the future. In conclusion, since the skin is an organ that is affected by both intrinsic aging and photoaging at the same time, when analyzing the expression of MMP-1 as a target for improving skin aging, it is necessary to select cells with a genotype suitable for the experimental conditions of the study.

• Atmosphere
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• v.23 no.2
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• pp.187-204
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• 2013

This study analyzed the synoptic distribution and vertical structure about four cases of precipitation occurrences using NCEP/NCAR reanalysis data and upper level data of winter intensive observation to be performed by National Institute of Meteorological Research at Bukgangneung, Incheon, Boseong during 63days from 4 JAN to 6 MAR in 2012, and Observing System Experiment (OSE) using 3DVAR-WRF system was conducted to examine the precipitation predictability of upper level data at western and southern coastal regions. The synoptic characteristics of selected precipitation occurrences were investigated as causes for 1) rainfall events with effect of moisture convergence owing to low pressure passing through south sea on 19 JAN, 2) snowfall events due to moisture inflowing from yellow sea with propagation of Siberian high pressure after low pressure passage over middle northern region on 31 JAN, 3) rainfall event with effect of weak pressure trough in west low and east high pressure system on 25 FEB, 4) rainfall event due to moisture inflow according to low pressures over Bohai bay and south eastern sea on 5 MAR. However, it is identified that vertical structure of atmosphere had different characteristics with heavy rainfall system in summer. Firstly, depth of convection was narrow due to absence of moisture convergence and strong ascending air current in middle layer. Secondly, warm air advection by veering wind with height only existed in low layer. Thirdly, unstable layer was limited in the narrow depth due to low surface temperature although it formed, and also values of instability indices were not high. Fourthly, total water vapor amounts containing into atmosphere was small due to low temperature distribution so that precipitable water vapor could be little amounts. As result of OSE conducting with upper level data of Incheon and Boseong station, 12 hours accumulated precipitation distributions of control experiment and experiments with additional upper level data were similar with ones of observation data at 610 stations. Although Equitable Threat Scores (ETS) were different according to cases and thresholds, it was verified positive influence of upper level data for precipitation predictability as resulting with high improvement rates of 33.3% in experiment with upper level data of Incheon (INC_EXP), 85.7% in experiment with upper level data of Boseong (BOS_EXP), and 142.9% in experiment with upper level data of both Incheon and Boseong (INC_BOS_EXP) about accumulated precipitation more than 5 mm / 12 hours on 31 January 2012.

• Journal of Veterinary Clinics
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• v.30 no.6
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• pp.490-495
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• 2013

A 9-year old, castrated male Pekingese dog weighing 6.9 kg was referred with one week history of dyspnea, depression, yellow vomiting and abdominal distension. Refractory right lung field opacity was diagnosed as spontaneous right cranial lung lobe torsion (LLT) with collapsed middle lobe on the computed tomography. Four days after diagnosis, right fourth intercostal thoracotomy was performed under propofol-remifentanil based total intravenous anesthesia (TIVA). Right cranial and middle lung lobes were twisted together in a clockwise direction. ENDOPATH-ETS-FLEX-45, an articulating endoscopic linear cutting stapler was applied at the hilus and there were no complications after resection. The patient recovered favorably and has been doing well up to one year follow up. Lung lobe torsion is an uncommon condition in small breed dogs, this case report suggests serial lung lobe torsion of cranial lobe followed by middle lobe in small breed dog, and immediately after diagnosis surgical intervention is beneficial to prevent progression of LLT. Furthermore, using self cutting stapling device and TIVA technique will be helpful for lung lobectomy in dogs.

• Asian-Australasian Journal of Animal Sciences
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• v.33 no.2
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• pp.187-196
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• 2020

Objective: Porcine respiratory disease is one of the most important health problems causing significant economic losses. To understand the genetic basis for susceptibility to swine enzootic pneumonia (EP) in pigs, we detected 102,809 single nucleotide polymorphisms in a total of 249 individuals based on genome-wide sequencing data. Methods: Genome comparison of susceptibility to swine EP in three pig breeds (Jinhua, Erhualian, and Meishan) with two western lines that are considered more resistant (Duroc and Landrace) using cross-population extended haplotype homozygosity and F-statistic (F_ST) statistical approaches identified 691 positively selected genes. Based on quantitative trait loci, gene ontology terms and literature search, we selected 14 candidate genes that have convincible biological functions associated with swine EP or human asthma. Results: Most of these genes were tested by several methods including transcription analysis and candidate genes association study. Among these genes: cytochrome P450 1A1 and catenin beta 1 (CTNNB1) are involved in fertility; transforming growth factor beta receptor 3 plays a role in meat quality traits; Wnt family member 2, CTNNB1 and transcription factor 7 take part in adipogenesis and fat deposition simultaneously; plasminogen activator, urokinase receptor (completely linked to AXL receptor tyrosine kinase, r² = 1) plays an essential role in the successful ovulation of matured oocytes in pigs; colipase like 2 (strongly linked to SAM pointed domain containing ETS transcription factor, r² = 0.848) is involved in male fertility. Conclusion: These adverse genes susceptible to swine EP may be selected while selecting for economic traits (especially reproduction traits) due to pleiotropic and hitchhiking effect of linked genes. Our study provided a completely new point of view to understand the genetic basis for susceptibility or resistance to swine EP in pigs thereby, provides insight for designing sustainable breed selection programs. Finally, the candidate genes are crucial due to their potential roles in respiratory diseases in a large number of species, including human.

• The Journal of Industrial Distribution & Business
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• v.10 no.6
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• pp.29-37
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• 2019

Purpose - This study intends to analyze Chinese consumers' purchase intention of Korean automobile. The aims of this study investigated to find out how product attributes and brand image affect the consumer's purchase intention of Korean automobiles in China. We consider the product attributes, brand image as evaluation criteria, also recognized as objectives for purchase intention. This study aims to analyze the positive effects on Chinese consumers' purchase intention with the variables by using the research model. Research design, data, and methodology - Based on theory of Purchase Intention, the concept of purchase intention was assessed by two factors: a) product attributes, and b) brand image to test the proposed hypotheses. The survey was conducted for 30 days, from October 17 to November 16 in 2018. With IBM SPSS Statistics 23.0 and IBM SPSS AMOS 23.0, factor analysis and regression, Cronbach's alpha and correlation were tested. 300 samples were used for this analysis. Results - As a main result, product attributes and brand image exerted significant effects on consumer's purchase intention. Thus,

and

were supported. The results showed that the product attributes, brand image had positive effects on purchase intention. The findings of this thesis are as follows: Firstly, automobiles' quality factors were divided into two categories like product properties (price, performance, design, economy, and safety), and brand image (awareness, favorability, reliability, and familiarity). Second, it showed that product properties and brand image had positive impacts on purchase intention of consumer. Conclusions - The impact of product attributes and brand image on consumer perceived value of Korean automobiles in China can be seen as a universal psychology of consumers who trust automobile products such as high quality, technology, ets. The results offer some insights into the extended model and have important managerial implications for Korean automobile enterprise. As the findings of this thesis show, all strategic orientations have significant and positive effects on the ability of innovation. At the same time, automobile company must pay attention to quality. As the relationship between the automobiles' performance and brand image were positive and significant, automobile companies should have comprehensive strategic orientations focused on strengthening these factors.

• KIPS Transactions on Computer and Communication Systems
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• v.6 no.4
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• pp.189-196
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• 2017

BIS(Bus Information System) provides the different information related to buses including predictions of arriving times at stations. BIS have been deployed almost all cities in our country and played active roles to improve the convenience of public transportation systems. Moving average filters, Kalman filter and regression models have been representative in forecasting the arriving times of buses in current BIS. The accuracy in prediction of arriving times depends largely on the forecasting algorithms and traffic conditions considered when forecasting in BIS. In present BIS, the simple prediction algorithms are used only considering the passage times and distances between stations. The forecasting of arrivals, however, have been influenced by the traffic conditions such as traffic signals, traffic accidents and pedestrians ets., and missing data. To improve the accuracy of bus arriving estimates, there are big troubles in building models including the above problems. Hidden Markov Models have been effective algorithms considering various restrictions above. So, we have built the HMM forecasting models for bus arriving times in the current BIS. When building models, the data collected from Sunchean City at 2015 have been utilized. There are about 2298 stations and 217 routes in Suncheon city. The models are developed differently week days and weekend. And then the models are conformed with the data from different districts and times. We find that our HMM models can provide more accurate forecasting than other existing methods like moving average filters, Kalmam filters, or regression models. In this paper, we propose Hidden Markov Model to obtain more precise and accurate model better than Moving Average Filter, Kalman Filter and regression model. With the help of Hidden Markov Model, two different sections were used to find the pattern and verified using Bootstrap process.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.31 no.1B
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• pp.29-36
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• 2011

Evapotranspiration (ET) from the various surfaces needs to be understood because it is a crucial hydrological factor to grasp interaction between the land surface and the atmosphere. A traditional way of estimating it, which is calculating it empirically using lysimeter and pan evaporation observations, has a limitation that the measurements represent only point values. However, these measurements cannot describe ET because it is easily affected by outer circumstances. Thus, remote sensing technology was applied to estimate spatial distribution of ET. In this study, we estimated major components of energy balance method (i.e. net radiation flux, soil heat flux, sensible heat flux, and latent heat flux) and ET as a map using Mapping Evapo-Transpiration with Internalized Calibration (METRIC) satellite-based image processing model. This model was run using Landsat imagery of Gyeongan watershed in Korea on Feb 1, 2003 and Sep 13, 2006. Basic statistical analyses were also conducted. The estimated mean daily ETs had respectively 22% and 11% of errors with pan evaporation data acquired from the Suwon Weather Station. This result represented similar distribution compared with previous studies and confirmed that the METRIC algorithm had high reliability in the watershed. In addition, ET distribution of each land use type was separately examined. As a result, it was identified that vegetation density had dominant impacts on distribution of ET. Seasonally, ET in a growing season represented significantly higher than in a dormant season due to more active transpiration. The ET maps will be useful to analyze how ET behaves along with the circumstantial conditions; land cover classification, vegetation density, elevation, topography.