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Comparison of residue patterns between foliar application and drenching in export strawberry (경엽 및 관주 처리에 따른 딸기 중 침투성 농약 잔류패턴 비교)

  • Lee, Hyo-Sub;Hong, Su-Myeong;Kwon, Hye-Young;Kim, Dan-bi;Moon, Byeong-Chul
    • Journal of Applied Biological Chemistry
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    • v.60 no.4
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    • pp.313-319
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    • 2017
  • Strawberries are one of the main commodities in Korea and have been exported over 30 million dollar. And the safety has recently become an important issue in the agro-trade, pesticide residue is most important matter of safe agricultural products. As strawberries can be cultivated using elevated production system, so it is possible to use the drenching. When spraying the pesticides, the use of drenching can be lower amount of residual than using foliar treatment. In this study, time sequential residual amount of pesticides were compared between drenching and foliar application in strawberries. Pesticides were treated with the standard and double dosage and samples were collected at 0, 3, 6, 9, 13, 16 and 21 day and analyzed by LC and LC-MS/MS after liquid-liquid extraction and QuEChERS. Two pesticides were detected less than 1 mg/kg in all samples for drenching treatment. Carbendazim residues in drenching treatment were 5 to 25 times lower than foliar application. And azoxystrobin was not detected in strawberries of drenching treatment.

Population density of chigger mites, the vector of tsutsugamushi disease in Chollanam-do, Korea (전라남도에서 쯔쯔가무시병 매개체인 털진드기의 개체군 밀도에 관한 조사)

  • 송현제;김개환
    • Parasites, Hosts and Diseases
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    • v.34 no.1
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    • pp.27-34
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    • 1996
  • The geographical distribution and population density of rodents and chigger mites at six localities of Chollanam-do were investigated from October to December in 1993. Among total 142 field rodents collected by the modified Chemin wooden traps, 131 rodents were Aponemw usrari,us (92.3%) and 11 were Crocidurc losiurc (7.7%). Out of 142 field rodents, 92 were parasitized by chiggers, showing 69.0% of the infestation rate and 74.2 of the chiggor index. Infestation rate and chigger index of A. omfernrius and C. lusiurc were 73.3%, 80.4 and 18.2%, 0.5, respectively. From the trapped field rodents, 10,532 chiggers were collected and identified with 11 species of 4 genera. Leptotrombidium pcLLinun. the vector 3pecies of tsutsugamushi disease, was the dominant species, showing 8,038 chi99ers (76.31%). L. scutellnre was the second dominant species showing 1,359 chiggers (12.9%). The distribution of chigger mites was clearly localized by the species, showing the different dominant species according to localities. The predominant species was 1. scutellare (100%) in Changhung-gun, 1. scutellcre (41.5%) in Posong-gun, 1. pnllidum (88.8%) in Hwasun- gun, 1. pnLlidunl (59.2%) in Koksong-gun, 1. zetum (77.3%) in Hampyong-gun, and 1. pclpcle (63.4%) in Tamyang-gun. Regarding to the geographical distribution of chigger population density, the infestation rate and chigger index was most high in Hwasun-gun as 62.4% and 216.2 respectively, and next high in Koksong-gun as 22.4% and 77.7%, respectively.

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Organotin Compounds Act as Inhibitor of Transcriptional Activation with Human Estrogen Receptor

  • Cho, Eun-Min;Lee, Haeng-Seog;Moon, Jeong-Suk;Kim, Im-Soon;Sim, Sang-Hyo;Ohta, Akinori
    • Journal of Microbiology and Biotechnology
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    • v.22 no.3
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    • pp.378-384
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    • 2012
  • In aquatic invertebrates, particularly marine gastropods, organotin compounds induce irreversible sexual abnormality in females, which is termed imposex, at very low concentrations. Organotin compounds are agonists for nuclear receptors such as RXRs and $PPAR{\gamma}$. However, the imposex phenomenon has not been reported to act as an antagonist on estrogen receptors in other species, including vertebrates and invertebrates. In order to gain insights into the antagonistic activity of organotin compounds on estrogen receptors (ERs), we examined the inhibitive effect of these compounds on estradiol-dependent ${\beta}$-galactosidase activity using the yeast two-hybrid detection system consisting of a combination of the human estrogen receptor ($hER{\beta}$) ligand-binding domain and the co-activator steroid receptor co-activator-1 (SRC1). Tributyltin-hydroxide (TBT-OH) and triphenyltin-chlorine (TPT-Cl) exhibited an inhibitive effect on $E_2$-dependent transcriptional activity, similar to antagonistic chemicals such as 4-hydroxytamoxifen (OHT) or ICI 182,780, at a very low concentration of $10^{-14}$ M TBT or $10^{-10}$ M TPT, respectively. The yeast growth and transcriptional activity with transcriptional factor GAL4 did not exhibit any effect at the tested concentration of TBT or TPT. Moreover, the yeast two-hybrid system using the interaction between p53 and the T antigen of SV40 large did not describe any effect at the tested concentration of OHT or ICI 182,780. However, the interaction between p53 and T antigen was inhibited at a TBT or TPT concentration of $10^{-9}$ M, respectively. These results indicate that TBT and TPT act as inhibitors of ER-dependent reporter gene transcriptional activation and of the interaction between $hER{\beta}$ LBD and the co-activator SRC1 in the yeast two-hybrid system. Consequently, our data could partly explain the occurrence of organotin compound-induced imposex on the endocrine system of mammals, including humans.

Study on the Optimization of DMB Image Contents Production Method (DMB 영상콘텐츠 제작기법의 최적화에 대한 연구)

  • Lim, Pyung-Jong;Kim, Jong-Seo;Kwak, Hoon-Sung
    • Journal of Digital Contents Society
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    • v.9 no.3
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    • pp.399-412
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    • 2008
  • The 21st century has been changed into a knowledge-oriented society, which means our society is more dependent on information and moves toward information. The mixing of broadcasting and co mmunication prevails and it makes possible a new type of broadcasting service. Depending on that, a broadcasting is being changed into a consumer-oriented service to satisfy the demands of consum ers in a new media age. It makes us attain to the personal media age to be possible interactive com munication unlike existing one-way transmission. As a result, new complex media are commercialize d and the instance is DMB. DMB is said 'my own TV' or 'TV in my own hands'. But it has limit t o retransmit existing broadcast programs. So hereafter, DMB broadcasting must be produced with co ntents suited its properties out of retransmitting existing contents. It is necessary to analyze exactly properties and service fields of DMB media to make an establish production direction of contents for DMB. This paper intends to suggest overall optimized image-contents production direction including appropriate program developments, a proper running time, visual expressions such as a camera angle and walking etc... DMB contents suited with its properties will give more familiarities to DMB users. Besides, DMB is expected to be a new culture watching broadcasting.

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GMPLS based Functional Models and Connection Admission Control Algorithms for Optical Burst Switched Networks (광 버스트 교환 망을 위한 GMPLS 기반 기능 모델과 연결 수락 제어 알고리즘)

  • So, Won-Ho;Roh, Sun-Sik;Kim, Young-Chon
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.29 no.9B
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    • pp.778-790
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    • 2004
  • In this paper, we propose the functional models of optical burst switching (OBS) routers to apply GMPLS (Generalized Multiprotocol Label Switching) to optical networks based on OBS. In addition, we introduce a connection admission control (CAC) algorithms which are operated in this models and can accommodate the required QoS. Firstly, the characteristics of current GMPLS and OBS for the optical Internet are basically considered. With this consideration, the models are proposed to accept OBS features which include the recognition of data bounda η with control information and the statistical multiplexing in terms of bursts. Secondly, we use an offset time decision (OTD) algorithm on behalf of controlling the connection admission with taking QoS parameters such as burst loss rate(BLR) and service-differentiation ratio(SDR) into consideration. The proposed CAC algorithms use the offered load of LSP (Label Switched Path), wavelength information, and QoS parame ‘ ers as inputs of OTD algorithm. A call setup request will be accepted when the offset time decided by OTD algorithm is reasonable for guaranteeing its requested QoS. Simulation is used for performance evaluation Results show the proposed schemes can guarantee the required QoS and those are better than the previous one in terms of channel utilization.

Evaluation of Cyantraniliprole Residues Translocated by Lettuce, Spinach and Radish (상추, 시금치 및 알타리무에 의한 Cyantraniliprole의 흡수이행 잔류량 평가)

  • Yoon, Ji Hyun;Lee, Seung Won;Lim, Da Jung;Kim, Seon Wook;Kim, In Seon
    • Korean Journal of Environmental Agriculture
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    • v.40 no.4
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    • pp.335-344
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    • 2021
  • BACKGROUND: Cyantraniliprole is a systemic diamide insecticide that has been used to control lepidopteran pests in agriculture. Cyantraniliprole has become an issue due to its potentiality of unexpectable contamination in rotational crop cultivation. Thus, studies on the evaluation of cyantraniliprole translocated from soil into rotational crops are required. METHODS AND RESULTS: Cyantraniliprole was treated at a yearly maximum application level onto bare soil under greenhouse conditions in two geographically different regions. Lettuce was transplanted and spinach and radish were sown onto the soil 30 and 60 days-plant back intervals (PBIs) after cyantraniliprole treatment. The QuEChERS method was modified and coupled with LC/MS/MS analysis to determine the residues of cyantraniliprole in soil and plant samples. The methods for sample preparation and instrumental conditions were validated to meet the criteria of Codex guidelines and were successful to determine cyantraniliprole quantitatively and qualitatively in the samples. Cyantraniliprole residues in lettuce samples were 0.01 mg/kg for PBI 60 and 0.02 mg/kg for PBI 30, respectively. The residues in spinach samples were 0.01 mg/kg for PBI 60 and 0.01~0.02 mg/kg for PBI 30, respectively. Less than limit of the quantitation (LOQ) level (0.01 mg/kg) of cyantraniliprole was observed in radish samples. The residues in the plant samples were found as the levels less than maximum residue limit (MRL) for leafy and root vegetables. CONCLUSION(S): This study suggests PBI 30~60 days for rotational cultivation of lettuce, spinach and radish in greenhouse soil treated with cyantraniliprole at a yearly maximum application level.

Investigation of Residual Organochlorine Pesticides in Apple and Pear Orchard Soil and Fruit (사과 및 배 과수원의 토양 및 과실 중 잔류성유기염소계 농약류 잔류량 조사)

  • Lim, Sung-Jin;Park, Jeong-Hwon;Ro, Jin-Ho;Lee, Min-Ho;Yoon, Hyo-In;Choi, Geun-Hyoung;Ryu, Song-Hee;Yu, Hye-Jin;Park, Byung-Jun
    • Korean Journal of Environmental Agriculture
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    • v.38 no.2
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    • pp.110-118
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    • 2019
  • BACKGROUND: Residual organochlorine pesticides (OCPs) are designated as persistent organic pollutants (POPs) by Stockholm Convention because they bioaccumulate through the food web, and pose a risk of causing adverse effect to human health and the environment. Apple and pear is economic crop in agriculture, and its cultivation area and yield has been increased. Therefore, we tried to investigate the OCPs residue in apple and pear orchard soils and fruits. METHODS AND RESULTS: Extraction and clean-up method were developed using the modified QuEChERS method for residual organochlorine pesticides (ROCPs) in apple and pear orchard soil and fruits. Recovery and limit of quantitation (LOQ) of ROCPs in soil and fruits were 75.4-101.4 and 76.9-93.4%, 0.03-0.21 and $0.6-1.2{\mu}g/kg$, respectively. Detected ROCPs in apple and pear orchard soil was 2,4-DDT, 4,4-DDD, 4,4-DDE, 4,4-DDT, and endosulfan sulfate, the residues were 2.2, 1.9-48.0, 1.3-84.1, 90.6-863.1, and $11.3-239.0{\mu}g/kg$, respectively. But five pesticides in all fruit samples were not detected. CONCLUSION: These results showed that ROCPs residues in apple and pear orchard soil had no effect on safety of agricultural products.

Residual Characteristics and Risk Assessments of Metalaxyl-M and Dinotefuran in Crown Daisy (Metalaxyl-M 및 dinotefuran 입제의 쑥갓 중 잔류 특성 및 위해성 평가)

  • Song, Min-Ho;Yu, Ji-Woo;Kim, Jinchan;Lee, Kwanghun;Ko, Rakdo;Keum, Young-Soo;Lee, Jiho
    • Korean Journal of Environmental Agriculture
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    • v.41 no.2
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    • pp.108-114
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    • 2022
  • BACKGROUND: This study was performed to determine residual characteristics of soil-treated metalaxyl-M and dinotefuran in crown daisy and to evaluate the risks from intake of the residual pesticides in the crop. METHODS AND RESULTS: The pesticide granules were treated in soil on two levels, and the plants samples were collected 51 days after seeding. The analytes were extracted and partitioned using the QuEChERS extraction packet (MgSO4 4 g, NaCl 1 g). The quantitative methods for metalaxyl-M and dinotefuran were validated in linearity, accuracy, and precision. Risk assessments of the pesticides were performed using Korea national nutrition statistics 2019. CONCLUSION(S): The residual concentrations of metalaxyl-M in crown daisy were 0.09-0.10 mg/kg (for the treatment at 6 kg/10 a) and 0.17-0.19 mg/kg (12 kg/10 a), respectively. The residual concentrations of dinotefuran in the crop were 0.53-0.75 mg/kg (3 kg/10 a) and 1.17-1.26 mg/kg (6 kg/10 a). The amounts of pesticides were less than MRL (Maximum Residue Limits) according to the Korean MFDS (Ministry of Food and Drug Safety). The HI (Hazard Index) of metalaxyl-M and dinotefuran for consumers was 0.0075% and 0.2250%, respectively. For females in the age between 50-64, the major consumer group, the HIs of the pesticides were <3%. Considering the consumption of crown daisy, they are not considered to be of toxicological concern.

Development of the Simultaneous Analysis of 36 Sulfonylurea Herbicide Residues in Agricultural Products Using LC-MS/MS (LC-MS/MS를 이용한 농산물 중 제초제 Sulfonylurea계 36종 동시 시험법 개발)

  • Su Jung Lee;Jung Mi Lee;Gui Hyun Jang;Hyun-Kyung Kim;Ji Young Kim
    • Korean Journal of Environmental Agriculture
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    • v.42 no.2
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    • pp.139-151
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    • 2023
  • Sulfonylurea herbicides are widely used in agriculture because they have a long residual period and high selectivity. An analytical method was developed using QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) technique for simultaneous determination of sulfonylurea herbicide residues in agricultural products by liquid chromatography tandem mass spectrometry and for establishment MRL (Maximum Residue Limit) of those herbicides in Korea. Extraction was performed using acetonitrile containing 0.1% formic acid with MgSO4 (anhydrous magnesium sulfate) and NaCl (sodium chloride) and the extract was cleaned up using MgSO4 and C18 (octadecyl). The matrix-matched calibration curves were composed of 7 concentration levels from 0.001 to 0.25 mg/kg and their coefficients of determination (R2) exceeded 0.99. The recoveries of three spiking levels (LOQ, 10LOQ, 50LOQ, n=5) were in the range of 71.7-114.9% with relative standard deviations of less than 20.0% for all the five agriculture products. All validation values met criteria of the European Union SANTE/11312/2021 guidelines and Food and Drug Safety Evaluation guidelines. Therefore, the proposed analytical method was accurate, effective, and sensitive for sulfonylurea herbicide residues determination in agricultural commodities.

A Study on the Structural Relationship between Employee Services and Store Loyalty (종업원 서비스와 점포충성도간의 구조적 관계에 관한 연구)

  • Yoon, Sung-Wook;Suh, Geun-Ha
    • Asia Marketing Journal
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    • v.6 no.3
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    • pp.59-81
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    • 2004
  • Store loyalty is increasingly being recognized as a path to long-term business profitability. Customer contact employees deliver a service firm's promises and create an important image for the firm. A major purpose of this study is to investigate the effects of customer service and product value on store loyalty. In order to test research hypotheses, data were collected through surveys administered to 300 apparel store customers. Two hundred thirty nine usable data were used for the analysis. The findings of this research are as follows: First, a employee's voluntary service(EVS) has a positive impact on interpersonal r elationship, which then affects switching barrier and store loyalty. Second, a employee's regular service(ERS) has an influence on store satisfaction, which in turn affect store loyalty. Third, product value is shown to be a significant antecedent to store satisfaction, which have a direct effect on store loyalty. The study concludes with implications, contributions, and limitations of the research and the empirical findings of this research should be beneficial to marketing practitioners and retailing businessmen in developing effective marketing strategies.

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