• 제목/요약/키워드: ER sensing

검색결과 20건 처리시간 0.025초

Endoplasmic Reticulum Ca2+ Store: Regulation of Ca2+ Release and Reuptake by Intracellular and Extracellular Ca2+ in Pancreatic Acinar Cells

  • Kang, Yun Kyung;Park, Myoung Kyu
    • Molecules and Cells
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    • 제19권2호
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    • pp.268-278
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    • 2005
  • We investigated the effect of cytosolic and extracellular $Ca^{2+}$ on $Ca^{2+}$ signals in pancreatic acinar cells by measuring $Ca^{2+}$ concentration in the cytosol($[Ca^{2+}]_c$) and in the lumen of the ER($[Ca^{2+}]_{Lu}$). To control buffers and dye in the cytosol, a patch-clamp microelectrode was employed. Acetylcholine released $Ca^{2+}$ mainly from the basolateral ER-rich part of the cell. The rate of $Ca^{2+}$ release from the ER was highly sensitive to the buffering of $[Ca^{2+}]_c$ whereas ER $Ca^{2+}$ refilling was enhanced by supplying free $Ca^{2+}$ to the cytosol with $[Ca^{2+}]_c$ clamped at resting levels with a patch pipette containing 10 mM BAPTA and 2 mM $Ca^{2+}$. Elevation of extracellular $Ca^{2+}$ to 10 mM from 1 mM raised resting $[Ca^{2+}]_c$ slightly and often generated $[Ca^{2+}]_c$ oscillations in single or clustered cells. Although pancreatic acinar cells are reported to have extracellular $Ca^{2+}$-sensing receptors linked to phospholipase C that mobilize $Ca^{2+}$ from the ER, exposure of cells to 10 mM $Ca^{2+}$ did not decrease $[Ca^{2+}]_{Lu}$ but rather raised it. From these findings we conclude that 1) ER $Ca^{2+}$ release is strictly regulated by feedback inhibition of $[Ca^{2+}]_c$, 2) ER $Ca^{2+}$ refilling is determined by the rate of $Ca^{2+}$ influx and occurs mainly in the tiny subplasmalemmal spaces, 3) extracellular $Ca^{2+}$-induced $[Ca^{2+}]_c$ oscillations appear to be triggered not by activation of extracellular $Ca^{2+}$-sensing receptors but by the ER sensitised by elevated $[Ca^{2+}]_c$ and $[Ca^{2+}]_{Lu}$.

Position estimation and control of SMA actuators based on electrical resistance measurement

  • Song, Gangbing;Ma, Ning;Lee, Ho-Jun
    • Smart Structures and Systems
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    • 제3권2호
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    • pp.189-200
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    • 2007
  • As a functional material, shape memory alloy (SMA) has attracted much attention and research effort to explore its unique properties and its applications in the past few decades. Some of its properties, in particular the electrical resistance (ER) based self-sensing property of SMA, have not been fully studied. Electrical resistance of an SMA wire varies during its phase transformation. This variation is an inherent property of the SMA wire, although it is highly nonlinear with hysteresis. The relationship between the displacement and the electrical resistance of an SMA wire is deterministic and repeatable to some degree, therefore enabling the self-sensing ability of the SMA. The potential of this self-sensing ability has not received sufficient exploration so far, and even the previous studies in literature lack generality. This paper concerns the utilization of the self-sensing property of a spring-biased Nickel-Titanium (Nitinol) SMA actuator for two applications: ER feedback position control of an SMA actuator without a position sensor, and estimation of the opening of a SMA actuated valve. The use of the self-sensing property eliminates the need for a position sensor, therefore reducing the cost and size of an SMA actuator assembly. Two experimental apparatuses are fabricated to facilitate the two proposed applications, respectively. Based on open-loop testing results, the curve fitting technique is used to represent the nonlinear relationships between the displacement and the electrical resistance of the two SMA wire actuators. Using the mathematical models of the two SMA actuators, respectively, a proportional plus derivative controller is designed for control of the SMA wire actuator using only electrical resistance feedback. Consequently, the opening of the SMA actuated valve can be estimated without using an extra sensor.

Electro-Micromechanical 시험법을 이용한 탄소섬유 강화 Epoxyacrylate 복합재료의 UV 및 열경화에 따른 비파괴적 손상 감지능 및 경화 Monitoring (Nondestructive Damage Sensing and Cure Monitoring of Carbon Fiber/Epoxyacrylate Composite with UV and Thermal Curing using Electro-Micromechanical Technique)

  • Kong, Jin-Woo;Kim, Dae-Sik;Park, Joung-Man;Lee, Jae-Rock
    • 한국복합재료학회:학술대회논문집
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    • 한국복합재료학회 2002년도 추계학술발표대회 논문집
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    • pp.261-264
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    • 2002
  • Interfacial evaluation, damage sensing and cure monitoring of single carbon fiber/thermosetting composite with different curing processes was investigated using electro-micromechanical test. After curing, residual stress was monitored by measurement of electrical resistance (ER) and then it was compared to correlate with various curing processes. In thermal curing, curing shrinkage appeared significantly by matrix shrinkage and residual stress due to the difference in thermal expansion coefficient (TEC). The change in electrical resistance (ΔR) on thermal curing was higher than that on ultraviolet (UV) curing. For thermal curing, apparent modulus was the highest and reaching time until same strain was faster. So far thermal curing shows strong durability on the IFSS after boiling test.

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Wireless 센서 네트워크를 위한 MAC 프로토콜에 관한 연구 (The Study of MAC protocol for efficient Wireless Sensor Network)

  • 이우철;김동일
    • 한국정보통신학회:학술대회논문집
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    • 한국해양정보통신학회 2005년도 추계종합학술대회
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    • pp.517-520
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    • 2005
  • 무선 센서망은 망의 사용목적에 따라 빛, 온도, 진동, 자기 영역, 바람 등을 감지 할 수 있는 센서와 컴퓨팅 기술로 통합된다. 무선 노드들은 센서에서 감지된 정보를 처리하는 신호 처리 기술과 측정자에게 정보가 도달하게 하는 통신 제어 기술과, 무선으로 구동되고 배터리를 사용하기 때문에 발생하는 제한적 에너지 관리 기술이 구현된다. 그 중 신뢰성 있는 측정 및 감지를 하고자 한다면 망의 지속시간의 비중이 크기 때문에 효율적인 에너지 관리 기술이 필요하다. 따라서 본 논문에서는 하드웨어에서의 전원관리가 아닌 OSI 7계층 중 데이터 링크 계층에 해당하는 MAC(Medium Access Control)에서 기존의 무선 MAC 프로토콜인 CSMA/CA(Carrier Sense Multiple Access/Collision Avoidance)과 무선 센서 MAC 프로토콜인 ER-MAC(Energy Rate Medium Access Control)에 대해 설명후 각각 프로토콜에 따른 망의 지속시간을 비교하여 효율성을 분석한다.

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Thermomechanical and electrical resistance characteristics of superfine NiTi shape memory alloy wires

  • Qian, Hui;Yang, Boheng;Ren, Yonglin;Wang, Rende
    • Smart Structures and Systems
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    • 제30권2호
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    • pp.183-193
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    • 2022
  • Structural health monitoring and structural vibration control are multidisciplinary and frontier research directions of civil engineering. As intelligent materials that integrate sensing and actuation capabilities, shape memory alloys (SMAs) exhibit multiple excellent characteristics, such as shape memory effect, superelasticity, corrosion resistance, fatigue resistance, and high energy density. Moreover, SMAs possess excellent resistance sensing properties and large deformation ability. Superfine NiTi SMA wires have potential applications in structural health monitoring and micro-drive system. In this study, the mechanical properties and electrical resistance sensing characteristics of superfine NiTi SMA wires were experimentally investigated. The mechanical parameters such as residual strain, hysteretic energy, secant stiffness, and equivalent damping ratio were analyzed at different training strain amplitudes and numbers of loading-unloading cycles. The results demonstrate that the detwinning process shortened with increasing training amplitude, while austenitic mechanical properties were not affected. In addition, superfine SMA wires showed good strain-resistance linear correlation, and the loading rate had little effect on their mechanical properties and electrical resistance sensing characteristics. This study aims to provide an experimental basis for the application of superfine SMA wires in engineering.

체지방 센서 노드(BSN) 설계 및 구현 (Design and Implementation of Body-fat sensing Node(BSN))

  • 김윤서;홍성웅;김평중
    • 한국정보처리학회:학술대회논문집
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    • 한국정보처리학회 2010년도 추계학술발표대회
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    • pp.1564-1566
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    • 2010
  • 본 논문에서는 유헬스 시스템에 가장 기본이 되는 생체정보 측정에 대하여 하나의 휴대용 인터페이스만을 가지고 다양한 생체정보 측정기와 연계하여 다양한 생체정보를 취득할 수 있도록 하는 이식성 있는 임베디드 미들웨어로써 특히 체지방측정을 위한 체지방 센서 노드(BSN)의 설계 및 구현에 관한 것이다. BSN은 U-health 서비스를 제공하기 위해 측정기 자동인식, 데이터 취득, 위험요소 표시 및 데이터 수집장치(DAS)와의 통신을 담당하는 기능을 갖는다.

ASIC2a-dependent increase of ASIC3 surface expression enhances the sustained component of the currents

  • Kweon, Hae-Jin;Cho, Jin-Hwa;Jang, Il-Sung;Suh, Byung-Chang
    • BMB Reports
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    • 제49권10호
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    • pp.542-547
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    • 2016
  • Acid-sensing ion channels (ASICs) are proton-gated cation channels widely expressed in the nervous system. Proton sensing by ASICs has been known to mediate pain, mechanosensation, taste transduction, learning and memory, and fear. In this study, we investigated the differential subcellular localization of ASIC2a and ASIC3 in heterologous expression systems. While ASIC2a targeted the cell surface itself, ASIC3 was mostly accumulated in the ER with partial expression in the plasma membrane. However, when ASIC3 was co-expressed with ASIC2a, its surface expression was markedly increased. By using bimolecular fluorescence complementation (BiFC) assay, we confirmed the heteromeric association between ASIC2a and ASIC3 subunits. In addition, we observed that the ASIC2a-dependent surface trafficking of ASIC3 remarkably enhanced the sustained component of the currents. Our study demonstrates that ASIC2a can increase the membrane conductance sensitivity to protons by facilitating the surface expression of ASIC3 through herteromeric assembly.

근전도와 안전도 기반의 인간-기계 인터페이스기술 (Development of Human-machine Interface based on EMG and EOG)

  • 강경우;김태선
    • 전자공학회논문지
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    • 제50권12호
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    • pp.129-137
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    • 2013
  • 최근 다양한 컴퓨터기반 장치들의 사용 확대에 따라 장애인에게도 정보접근의 용이성 확보가 요구되고 있다. 특히 사지를 움직일 수 없는 중증 장애인들을 위한 새로운 입력 인터페이스 기술의 개발이 절실히 요구된다. 이를 위해 본 논문에서는 중증 장애인이 활용 가능한 눈동자 움직임 및 깨물기를 통한 안전도(EOG)와 근전도(EMG)신호를 이용하여 아홉 가지의 명령이 가능한 인터페이스 시스템을 개발하였다. 구현된 시스템은 하드웨어로 구성된 센서모듈과 특징추출 및 패턴분류를 포함하는 소프트웨어부로 구성된다. 개발된 기술은 사용자의 편의성을 극대화하기 위해 두 개의 전극만을 이마부위에 부착하여 안전도와 근전도를 동시에 측정하고, 이를 통해 아홉 가지의 명령전달이 가능하도록 하였다. 성능평가를 위한 java 기반 실시간 모니터링 프로그램의 실험결과 92.52%의 인식률을 보였다. 또한, 구현된 시스템의 실용성을 입증하기 위해 다섯 종류의 명령을 이용하여 ER1 로봇의 조종에 성공적으로 적용 가능함을 보임으로써 장애인을 위한 새로운 인터페이스로서의 가능성을 보였다.

어븀 첨가 광섬유형 링 레이저와 광섬유 격자 기반 센서를 이용한 원거리의 온도 및 전류 측정 (Remote Measurement of a Distant Temperature and Current using Fiber Bragg Grating Sensors and Erbium-doped Fiber Ring Laser)

  • 손경락;심준환;양규식
    • Journal of Advanced Marine Engineering and Technology
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    • 제32권8호
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    • pp.1257-1262
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    • 2008
  • A long-distance remote sensing of temperature and current based on a fiber Bragg grating (FBG) is proposed and demonstrated. The thermal expanding effect of the epoxy and the Er-doped fiber ring laser (EFRL) are applied to the sensor system to enhance the temperature and current sensitivity. An EFRL with a 5 km-single-mode fiber and a FBG shows a high extinction ratio of more than 60 dB and a low power fluctuation of less than 1 dB. The metal wires are used to supply the current to the sensors. When the NOA65 puts on the FBG as a thermal expanding material, the temperature and current sensitivity of the lasing wavelength shift are about $30\;pm/^{\circ}C$ and 3pm/mA, respectively. The proposed sensing scheme is useful for measurement of current or temperature at a distant object of more than several km.

연필심을 이용한 종이센서에 의한 단일 랩 전단변형률 감지능 (Strain Sensing of Single Lap Shear using Pencil Lead Drawn Paper Sensor (PLDPS))

  • 유지훈;신평수;김종현;이상일;박종만
    • Composites Research
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    • 제33권4호
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    • pp.228-233
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    • 2020
  • 본 논문은 유리섬유강화 복합재료(GFRC)를 사용하여 단일 랩 전단 시험을 진행하고, 단일 랩 전단 시험을 진행하면서 연필심을 이용한 종이센서(PLDPS)를 적용하였다. 복합재료의 접착은 비스페놀-A계 에폭시와 아민계 경화제를 혼합하여 접착제로 사용하였다. 접착물성의 차이를 주기 위해 각기 다른 조건에서 경화시켰고 물성이 달라진 것을 확인하였다. 연필심을 이용한 종이센서는 A4용지에 4B연필을 사용하여 제작하였다. 연필에 있는 흑연은 전기가 통하는 물질이므로 전기저항을 측정할 수 있다. 연필심을 이용한 종이센서에 단일 랩 전단 시편에 붙은 위치에 따라 전기저항의 변화를 관찰하였고, 붙이는 두 위치에 따라 전기저항의 변화가 다르게 나오는 것을 확인하였다. 또한, 랩 전단변형률에 따라서 전기 저항의 변화도 관찰하였다. 랩 전단변형률이 높으면 전기 저항 변화도 큰 것을 확인했다. 접착부분의 변형이 클수록 시편이 휘는 정도도 커져 전극 사이의 거리변화도 크게 만들기 때문이다.